{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:03:45Z","timestamp":1788807825592,"version":"build-2803163510"},"reference-count":87,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG040288"],"award-info":[{"award-number":["AG040288"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG052732"],"award-info":[{"award-number":["AG052732"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM135767"],"award-info":[{"award-number":["GM135767"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008623","name":"Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008623","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Metabolism participates in the control of stem cell function and subsequent maintenance of tissue homeostasis. How this is achieved in the context of adult stem cell niches in coordination with other local and intrinsic signaling cues is not completely understood. The Target of Rapamycin (TOR) pathway is a master regulator of metabolism and plays essential roles in stem cell maintenance and differentiation. In the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    male germline, mTORC1 is active in germline stem cells (GSCs) and early germ cells. Targeted RNAi-mediated downregulation of mTor in early germ cells causes a block and\/or a delay in differentiation, resulting in an accumulation of germ cells with GSC-like features. These early germ cells also contain unusually large and dysfunctional autolysosomes. In addition, downregulation of mTor in adult male GSCs and early germ cells causes non-autonomous activation of mTORC1 in neighboring cyst cells, which correlates with a disruption in the coordination of germline and somatic differentiation. Our study identifies a previously uncharacterized role of the TOR pathway in regulating male germline differentiation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0300337","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T13:33:26Z","timestamp":1711028006000},"page":"e0300337","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["mTORC1 is required for differentiation of germline stem cells in the Drosophila melanogaster testis"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4992-1524","authenticated-orcid":true,"given":"Marie","family":"Cl\u00e9mot","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"D\u2019Alterio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3733-586X","authenticated-orcid":true,"given":"Alexa C.","family":"Kwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5721-6310","authenticated-orcid":true,"given":"D. Leanne","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"key":"pone.0300337.ref001","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.stem.2010.01.011","article-title":"Stem cells and the niche: a dynamic duo.","volume":"6","author":"J Voog","year":"2010","journal-title":"Cell Stem Cell"},{"key":"pone.0300337.ref002","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.cell.2014.02.013","article-title":"Nice neighborhood: emerging concepts of the stem cell niche","volume":"157","author":"DT Scadden","year":"2014","journal-title":"Cell"},{"key":"pone.0300337.ref003","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.cell.2008.01.038","article-title":"Stem Cells and Niches: Mechanisms That Promote Stem Cell Maintenance throughout Life.","author":"SJ Morrison","year":"2008","journal-title":"Cell. 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