{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T13:33:28Z","timestamp":1793194408278,"version":"4.5.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T00:00:00Z","timestamp":1714003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["SA097P20"],"award-info":[{"award-number":["SA097P20"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["FPU21\/00446"],"award-info":[{"award-number":["FPU21\/00446"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>One of the most common forms of cancer in fair skinned populations is Non-Melanoma Skin Cancer (NMSC), which primarily consists of Basal Cell Carcinoma (BCC), and cutaneous Squamous Cell Carcinoma (SCC). Detecting NMSC early can significantly improve treatment outcomes and reduce medical costs. Similarly, Actinic Keratosis (AK) is a common skin condition that, if left untreated, can develop into more serious conditions, such as SCC. Hyperspectral imagery is at the forefront of research to develop non-invasive techniques for the study and characterisation of skin lesions. This study aims to investigate the potential of near-infrared hyperspectral imagery in the study and identification of BCC, SCC and AK samples in comparison with healthy skin. Here we use a pushbroom hyperspectral camera with a spectral range of \u2248 900 to 1600 nm for the study of these lesions. For this purpose, an ad hoc platform was developed to facilitate image acquisition. This study employed robust statistical methods for the identification of an optimal spectral window where the different samples could be differentiated. To examine these datasets, we first tested for the homogeneity of sample distributions. Depending on these results, either traditional or robust descriptive metrics were used. This was then followed by tests concerning the homoscedasticity, and finally multivariate comparisons of sample variance. The analysis revealed that the spectral regions between 900.66\u20131085.38 nm, 1109.06\u20131208.53 nm, 1236.95\u20131322.21 nm, and 1383.79\u20131454.83 nm showed the highest differences in this regard, with &lt;1% probability of these observations being a Type I statistical error. Our findings demonstrate that hyperspectral imagery in the near-infrared spectrum is a valuable tool for analyzing, diagnosing, and evaluating non-melanoma skin lesions, contributing significantly to skin cancer research.<\/jats:p>","DOI":"10.1371\/journal.pone.0300400","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T13:46:18Z","timestamp":1714052778000},"page":"e0300400","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Near-infrared hyperspectral imaging and robust statistics for in vivo non-melanoma skin cancer and actinic keratosis characterisation"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4810-2001","authenticated-orcid":true,"given":"Lloyd A.","family":"Courtenay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1049-7586","authenticated-orcid":true,"given":"Innes","family":"Barbero-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saray","family":"Mart\u00ednez-Lastras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Del Pozo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Corral de la Calle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alonso","family":"Garrido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Guerrero-Sevilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9874-5243","authenticated-orcid":true,"given":"David","family":"Hernandez-Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Gonz\u00e1lez-Aguilera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"issue":"1","key":"pone.0300400.ref001","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jaad.2016.07.045","article-title":"Impact of skin cancer screening and secondary prevention campaigns on skin cancer incidence and mortality: A systematic review","volume":"76","author":"A. Brunssen","year":"2017","journal-title":"Journal of the American Academy of Dermatology"},{"issue":"7","key":"pone.0300400.ref002","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph17072321","article-title":"Non-Melanoma Skin Cancer in Outdoor Workers: A Study on Actinic Keratosis in Italian Navy Personnel.","volume":"17","author":"L. Vimercati","year":"2020","journal-title":"International Journal of Environmental Research and Public Health"},{"issue":"2","key":"pone.0300400.ref003","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.jaad.2021.03.109","article-title":"United States burden of melanoma and non-melanoma skin cancer from 1990 to 2019.","volume":"85","author":"P. Aggarwal","year":"2021","journal-title":"Journal of the American Academy of Dermatology"},{"issue":"1","key":"pone.0300400.ref004","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s12325-019-01130-1","article-title":"Recognising Skin Cancer in Primary Care.","volume":"37","author":"O. T. Jones","year":"2020","journal-title":"Advances in Therapy"},{"issue":"1","key":"pone.0300400.ref005","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1080\/07853890.2022.2154382","article-title":"Trends of non-melanoma skin cancer incidence in Hong Kong and projection up to 2030 based on changing demographics","volume":"55","author":"Q. Xu","year":"2023","journal-title":"Annals of Medicine"},{"issue":"1","key":"pone.0300400.ref006","article-title":"The incidence and clinical analysis of non-melanoma skin cancer","volume":"11","author":"M. Ci\u0105\u017cy\u0144ska","year":"2021","journal-title":"Scientific Reports"},{"issue":"6","key":"pone.0300400.ref007","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1111\/bjd.14477","article-title":"Is early detection of basal cell carcinoma worthwhile? Systematic review based on the WHO criteria for screening","volume":"174","author":"I. Hoorens","year":"2016","journal-title":"The British Journal of Dermatology"},{"issue":"9715","key":"pone.0300400.ref008","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S0140-6736(09)61196-X","article-title":"Non-melanoma skin cancer","volume":"375","author":"V. Madan","year":"2010","journal-title":"The Lancet"},{"issue":"7","key":"pone.0300400.ref009","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1002\/jso.23731","article-title":"The incidence and risk factors of metastasis for cutaneous squamous cell carcinoma\u2014Implications on the T-classification system","volume":"110","author":"N. D. L. Brougham","year":"2014","journal-title":"Journal of Surgical Oncology"},{"issue":"5","key":"pone.0300400.ref010","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1111\/jdv.12848","article-title":"is the most common lesion associated with invasive squamous cell carcinoma of the skin","volume":"29","author":"M. T. 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Glogau","year":"2000","journal-title":"Journal of the American Academy of Dermatology"},{"issue":"8589","key":"pone.0300400.ref014","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S0140-6736(88)91658-3","article-title":"Malignant transformation of solar keratoses to squamous cell carcinoma","volume":"1","author":"R. Marks","year":"1998","journal-title":"Lancet (London, England)."},{"issue":"8","key":"pone.0300400.ref015","doi-asserted-by":"crossref","DOI":"10.2340\/00015555-3486","article-title":"Actinic Keratosis Diagnosis and Increased Risk of Developing Skin Cancer: A 10-year Cohort Study of 17,651 Patients in Sweden.","volume":"100","author":"G. Guorgis","year":"2020","journal-title":"Acta Dermato-Venereologica."},{"issue":"4","key":"pone.0300400.ref016","first-page":"404","article-title":"The state of melanoma: Challenges and opportunities. Pigment Cell &","volume":"29","author":"G. Merlino","year":"2016","journal-title":"Melanoma Research"},{"key":"pone.0300400.ref017","article-title":"Dermoscopy in the diagnosis and management of non-melanoma skin cancers","author":"M. C. Fargnoli","year":"2012","journal-title":"Article in European Journal of Dermatology"},{"key":"pone.0300400.ref018","doi-asserted-by":"crossref","DOI":"10.3389\/fmed.2019.00180","article-title":"Dermoscopy of Melanoma and Non-melanoma Skin Cancers.","volume":"6","author":"J. Kato","year":"2019","journal-title":"Frontiers in Medicine"},{"issue":"3","key":"pone.0300400.ref019","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S1470-2045(02)00679-4","article-title":"Diagnostic accuracy of dermoscopy","volume":"3","author":"H. Kittler","year":"2002","journal-title":"The Lancet Oncology"},{"issue":"11","key":"pone.0300400.ref020","doi-asserted-by":"crossref","DOI":"10.1038\/nnano.2009.326","article-title":"Second window for in vivo imaging","volume":"4","author":"A. M. Smith","year":"2009","journal-title":"Nature Nanotechnology"},{"issue":"6","key":"pone.0300400.ref021","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1046\/j.1523-1747.1999.00612.x","article-title":"Infrared Spectra of Basal Cell Carcinomas are Distinct from Non-Tumor-Bearing Skin Components","volume":"112","author":"L. M. McIntosh","year":"1999","journal-title":"Journal of Investigative Dermatology"},{"issue":"1","key":"pone.0300400.ref022","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1046\/j.1523-1747.2001.00212.x","article-title":"Towards non-invasive screening of skin lesions by near-infrared spectroscopy","volume":"116","author":"L. M. McIntosh","year":"2001","journal-title":"Journal of Investigative Dermatology"},{"issue":"3","key":"pone.0300400.ref023","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1054\/bjps.1997.0088","article-title":"Assessment of tissue viability using near-infrared spectroscopy","volume":"51","author":"M. F. Stranc","year":"1998","journal-title":"British Journal of Plastic Surgery"},{"issue":"6","key":"pone.0300400.ref024","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.3390\/jcm9061662","article-title":"Non-Invasive Skin Cancer Diagnosis Using Hyperspectral Imaging for In-Situ Clinical Support.","volume":"9","author":"R. Leon","year":"2020","journal-title":"Journal of Clinical Medicine"},{"issue":"8","key":"pone.0300400.ref025","doi-asserted-by":"crossref","first-page":"5107","DOI":"10.1364\/BOE.428143","article-title":"Hyperspectral imaging and robust statistics in non-melanoma skin cancer analysis","volume":"12","author":"L. A. Courtenay","year":"2021","journal-title":"Biomedical Optics Express"},{"key":"pone.0300400.ref026","first-page":"100244G","author":"L. A. Zherdeva","year":"2016","journal-title":"In vivo hyperspectral imaging and differentiation of skin cancer"},{"issue":"1","key":"pone.0300400.ref027","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jbio.201960080","article-title":"Multispectral near infrared absorption imaging for histology of skin cancer","volume":"13","author":"A. Spreinat","year":"2020","journal-title":"Journal of Biophotonics"},{"issue":"6","key":"pone.0300400.ref028","doi-asserted-by":"crossref","first-page":"064026","DOI":"10.1117\/1.2398928","article-title":"Optical properties of normal and cancerous human skin in the visible and near-infrared spectral range","volume":"11","author":"E. Salomatina","year":"2006","journal-title":"Journal of Biomedical Optics"},{"key":"pone.0300400.ref029","doi-asserted-by":"crossref","unstructured":"Truong, B. C. Q., Tuan, H. D., & Nguyen, H. T. Near-infrared parameters extraction: A potential method to detect skin cancer. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013; 33\u201336. https:\/\/doi.org\/10.1109\/EMBC.2013.6609430.","DOI":"10.1109\/EMBC.2013.6609430"},{"issue":"2","key":"pone.0300400.ref030","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.chemolab.2004.01.023","article-title":"Hyperspectral imaging: Calibration problems and solutions.","volume":"72","author":"P. Geladi","year":"2004","journal-title":"Chemometrics and Intelligent Laboratory Systems"},{"key":"pone.0300400.ref031","volume-title":"Statistical power analysis for behavioural sciences","author":"J. Cohen","year":"1988"},{"key":"pone.0300400.ref032","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1070\/SM1967v001n04ABEH001994","article-title":"Distribution of eigenvalues for some sets of random matrices.","volume":"1","author":"V. A. Mar\u010denko","year":"1967","journal-title":"Mathematics of the USSR Sbornik"},{"issue":"4","key":"pone.0300400.ref033","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1002\/j.1538-7305.1948.tb00917.x","article-title":"A mathematical theory of communication.","volume":"27","author":"C. E. Shannon","year":"1948","journal-title":"The Bell System Technical Journal."},{"issue":"3","key":"pone.0300400.ref034","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/j.1538-7305.1948.tb01338.x","article-title":"A mathematical theory of communication.","volume":"27","author":"C. E. Shannon","year":"1948","journal-title":"The Bell System Technical Journal"},{"issue":"1","key":"pone.0300400.ref035","first-page":"21","article-title":"Power Comparisons of Shapiro-Wilk, Kolmogorov-Smirnov, Lilliefors and Anderson-Darling Tests","volume":"2","author":"N. M. Razali","year":"2011","journal-title":"Journal of Statistical Modeling and Analytics"},{"issue":"4","key":"pone.0300400.ref036","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.isprsjprs.2009.02.003","article-title":"Accuracy assessment of digital elevation models by means of robust statistical methods","volume":"64","author":"J. H\u00f6hle","year":"2009","journal-title":"ISPRS Journal of Photogrammetry and Remote Sensing"},{"issue":"18","key":"pone.0300400.ref037","article-title":"A New Method for Positional Accuracy Control for Non-Normal Errors Applied to Airborne Laser Scanner Data.","volume":"9","author":"F. J. Ariza-L\u00f3pez","year":"2019","journal-title":"Applied Sciences."},{"issue":"10","key":"pone.0300400.ref038","doi-asserted-by":"crossref","first-page":"e0240328","DOI":"10.1371\/journal.pone.0240328","article-title":"Obtaining new resolutions in carnivore tooth pit morphological analyses: A methodological update for digital taphonomy.","volume":"15","author":"L. A. Courtenay","year":"2020","journal-title":"PLOS ONE."},{"issue":"3","key":"pone.0300400.ref039","first-page":"5497","article-title":"The use of LIDAR as a data source for digital elevation models\u2013a study of the relationship between the accuracy of digital elevation models and topographical attributes in northern peatlands.","volume":"8","author":"A. Hasan","year":"2011","journal-title":"Hydrology and Earth System Sciences Discussions."},{"issue":"4","key":"pone.0300400.ref040","doi-asserted-by":"crossref","first-page":"e0196004","DOI":"10.1371\/journal.pone.0196004","article-title":"Automatic tree parameter extraction by a Mobile LiDAR System in an urban context.","volume":"13","author":"M. Herrero-Huerta","year":"2018","journal-title":"PLOS ONE."},{"issue":"19","key":"pone.0300400.ref041","doi-asserted-by":"crossref","DOI":"10.3390\/rs11192205","article-title":"Validation of Portable Mobile Mapping System for Inspection Tasks in Thermal and Fluid\u2013Mechanical Facilities.","volume":"11","author":"M. Rodr\u00edguez-Mart\u00edn","year":"2019","journal-title":"Remote Sensing"},{"issue":"901","key":"pone.0300400.ref042","first-page":"268","article-title":"Properties of sufficiency and statistical testsProceedings of the Royal Society of London.","volume":"160","author":"M. S. Bartlett","year":"1997","journal-title":"Series A\u2014Mathematical and Physical Sciences."},{"key":"pone.0300400.ref043","unstructured":"Levene, H. Robust testes for equality of variances in Contributions to Probability and Statistics.1960; 278\u2013292. Stanford Univ. Press, Palo Alto, CA. MR0120709."},{"key":"pone.0300400.ref044","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1525\/9780520411586-004","article-title":"A Generalized T Test and Measure of Multivariate Dispersion","volume":"2","author":"H. Hotelling","year":"1951","journal-title":"Proceedings of the Second Berkeley Symposium on Mathematical Statistics and Probability"},{"key":"pone.0300400.ref045","volume-title":"Nonparametric Statistical Methods.","author":"M. Hollander","year":"2013"},{"key":"pone.0300400.ref046","unstructured":"Gunn, S. Support vector machines for classification and regression. ISIS Technical. 1998. Report. https:\/\/www.svms.org\/tutorials\/Gunn1998.pdf."},{"issue":"2","key":"pone.0300400.ref047","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1080\/00031305.2016.1154108","article-title":"The ASA Statement on p-Values: Context, Process, and Purpose","volume":"70","author":"R. L. Wasserstein","year":"2016","journal-title":"The American Statistician"},{"issue":"sup1","key":"pone.0300400.ref048","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00031305.2019.1583913","article-title":"Moving to a World Beyond \u201cp < 0.05\u201d.","volume":"73","author":"R. L. Wasserstein","year":"2019","journal-title":"The American Statistician"},{"issue":"12","key":"pone.0300400.ref049","doi-asserted-by":"crossref","first-page":"171085","DOI":"10.1098\/rsos.171085","article-title":"The reproducibility of research and the misinterpretation of p-values","volume":"4","author":"D. Colquhoun","year":"2017","journal-title":"Royal Society Open Science"},{"issue":"sup1","key":"pone.0300400.ref050","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1080\/00031305.2018.1543135","article-title":"Three Recommendations for Improving the Use of p-Values","volume":"73","author":"D. J. Benjamin","year":"2019","journal-title":"The American Statistician"},{"issue":"11","key":"pone.0300400.ref051","doi-asserted-by":"crossref","first-page":"117003","DOI":"10.1117\/1.JBO.19.11.117003","article-title":"Clinical study of noninvasive in vivo melanoma and nonmelanoma skin cancers using multimodal spectral diagnosis","volume":"19","author":"L. Lim","year":"2014","journal-title":"Journal of Biomedical Optics"},{"issue":"1","key":"pone.0300400.ref052","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/1523-1747.ep12479191","article-title":"The Optics of Human Skin","volume":"77","author":"R. R. Anderson","year":"1981","journal-title":"Journal of Investigative Dermatology"},{"issue":"9","key":"pone.0300400.ref053","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.3390\/cancers11091367","article-title":"Hyperspectral Imaging of Head and Neck Squamous Cell Carcinoma for Cancer Margin Detection in Surgical Specimens from 102 Patients Using Deep Learning.","volume":"11","author":"M. Halicek","year":"2019","journal-title":"Cancers"},{"issue":"1","key":"pone.0300400.ref054","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3390\/biomedicines6010008","article-title":"Actinic Keratosis and Non-Invasive Diagnostic Techniques: An Update.","volume":"6","author":"A. Casari","year":"2018","journal-title":"Biomedicines."},{"issue":"3","key":"pone.0300400.ref055","doi-asserted-by":"crossref","first-page":"130","DOI":"10.3322\/canjclin.35.3.130","article-title":"Early Detection of Malignant Melanoma: The Role of Physician Examination and Self-Examination of the Skin. CA","volume":"35","author":"R. J. Friedman","year":"1985","journal-title":"A Cancer Journal for Clinicians"},{"key":"pone.0300400.ref056","volume-title":"An illustrated guide to the recognition of ealy malignant melanoma","author":"R. M. MacKie","year":"1986"},{"issue":"15","key":"pone.0300400.ref057","doi-asserted-by":"crossref","DOI":"10.3390\/jcm11154392","article-title":"A Novel Approach for the Shape Characterisation of Non-Melanoma Skin Lesions Using Elliptic Fourier Analyses and Clinical Images","volume":"11","author":"L. A. 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