{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:41:15Z","timestamp":1790638875511,"version":"4.1.0"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T00:00:00Z","timestamp":1712275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP52155"],"award-info":[{"award-number":["OPP52155"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"\n Introduction<\/jats:title>\n \n Extended-spectrum beta-lactamase (ESBL)-producing\n Escherichia coli<\/jats:italic>\n and\n Klebsiella pneumoniae<\/jats:italic>\n are pathogens of significant public health interest for which new antibiotics are urgently needed.\n <\/jats:p>\n <\/jats:sec>\n \n Aim<\/jats:title>\n \n To determine the prevalence of ESBLs in\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n and\n K<\/jats:italic>\n .\n pneumoniae<\/jats:italic>\n isolates from patients attending the Tamale Teaching Hospital (TTH) in Ghana.\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n The study was a cross-sectional study involving convenience sampling of\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n and\n K<\/jats:italic>\n .\n pneumoniae<\/jats:italic>\n isolates from consenting patients\u2019 clinical specimens, between April and June 2015. Antimicrobial susceptibility test was performed, and ESBL-producer phenotypes were further screened for\n Bla<\/jats:italic>\n TEM<\/jats:sub>\n ,\n Bla<\/jats:italic>\n SHV<\/jats:sub>\n , and\n Bla<\/jats:italic>\n CTX-M<\/jats:sub>\n genes. Patients\u2019 clinical data were additionally collected using a structured questionnaire.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Of the 150 non-duplicate\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n and\n K<\/jats:italic>\n .\n pneumoniae<\/jats:italic>\n isolates identified, 140 were confirmed as\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n (84%,\n n<\/jats:italic>\n = 117) and\n K<\/jats:italic>\n .\n pneumoniae<\/jats:italic>\n (16%,\n n<\/jats:italic>\n = 23). Of these, sixty-two (44%) [\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n (84%;\n n<\/jats:italic>\n = 52);\n K<\/jats:italic>\n .\n pneumoniae<\/jats:italic>\n (16%;\n n<\/jats:italic>\n = 10)] phenotypically expressed ESBLs. The proportion of ESBL-producing isolates was higher in adults (15\u201365 years) than in neonates (< 28 days) (\n p =<\/jats:italic>\n 0.14). Most of the isolates showed a high percentage resistance to ampicillin (96%) and tetracycline (89%), but a relatively lower resistance to amikacin (36%). No isolate was resistant to meropenem. More ESBL producers were multidrug resistant compared to non-ESBL-producers [23% (14\/62) versus 18% (14\/78);\n p<\/jats:italic>\n = 0.573]. Overall, 74% (\n n<\/jats:italic>\n = 46) of the ESBL genotypes expressed\n Bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n genes, followed by 63% (\n n<\/jats:italic>\n = 39)\n Bla<\/jats:italic>\n TEM<\/jats:sub>\n , and 16% (\n n<\/jats:italic>\n = 10)\n Bla<\/jats:italic>\n SHV<\/jats:sub>\n . The study showed a high prevalence of ESBL-positive\n E<\/jats:italic>\n .\n coli<\/jats:italic>\n and\n K<\/jats:italic>\n .\n pneumoniae<\/jats:italic>\n , mostly CTX-M-1 producers at TTH.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Routine laboratory ESBL screening is warranted to inform patient management.<\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0300596","type":"journal-article","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T12:21:21Z","timestamp":1712319681000},"page":"e0300596","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae recovered from patients at the Tamale Teaching Hospital, Ghana"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-8320","authenticated-orcid":true,"given":"Francis Kwame 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