{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T03:49:19Z","timestamp":1784260159479,"version":"3.55.0"},"reference-count":125,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018948","name":"Saudi Arabian Cultural Bureau","doi-asserted-by":"publisher","award":["IR12012\/2 and IR1610"],"award-info":[{"award-number":["IR12012\/2 and IR1610"]}],"id":[{"id":"10.13039\/100018948","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017564","name":"Ci\u00eancia sem Fronteiras","doi-asserted-by":"publisher","award":["BEX-13070-13-4"],"award-info":[{"award-number":["BEX-13070-13-4"]}],"id":[{"id":"10.13039\/501100017564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>During the oestrous cycle, the bovine endometrium undergoes morphological and functional changes, which are regulated by alterations in the levels of oestrogen and progesterone and consequent changes in gene expression. To clarify these changes before and after oestrus, RNA-seq was used to profile the transcriptome of oestrus-synchronized beef heifers. Endometrial samples were collected from 29 animals, which were slaughtered in six groups beginning 12 h after the withdrawal of intravaginal progesterone releasing devices until seven days post-oestrus onset (luteal phase). The groups represented proestrus, early oestrus, metoestrus and early dioestrus (luteal phase). Changes in gene expression were estimated relative to gene expression at oestrus. Ingenuity Pathway Analysis (IPA) was used to identify canonical pathways and functional processes of biological importance. A total of 5,845 differentially expressed genes (DEGs) were identified. The lowest number of DEGs was observed at the 12 h post-oestrus time point, whereas the greatest number was observed at Day 7 post-oestrus onset (luteal phase). A total of 2,748 DEGs at this time point did not overlap with any other time points. Prior to oestrus,<jats:italic>Neurological disease<\/jats:italic>and<jats:italic>Organismal injury and abnormalities<\/jats:italic>appeared among the top IPA diseases and functions categories, with upregulation of genes involved in neurogenesis. Lipid metabolism was upregulated before oestrus and downregulated at 48h post-oestrus, at which point an upregulation of immune-related pathways was observed. In contrast, in the luteal phase the<jats:italic>Lipid metabolism<\/jats:italic>and<jats:italic>Small molecule biochemistry pathways<\/jats:italic>were upregulated.<\/jats:p>","DOI":"10.1371\/journal.pone.0301005","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T17:34:52Z","timestamp":1711647292000},"page":"e0301005","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Transcriptomics analysis of the bovine endometrium during the perioestrus period"],"prefix":"10.1371","volume":"19","author":[{"given":"Mohammed A.","family":"Alfattah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0821-8245","authenticated-orcid":true,"given":"Carolina N.","family":"Correia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John 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