{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T05:01:11Z","timestamp":1748926871209,"version":"3.40.5"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T00:00:00Z","timestamp":1713312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH National Eye Institute","award":["EY021747"],"award-info":[{"award-number":["EY021747"]}]},{"name":"NIH National Eye Institute","award":["P30 EY031631"],"award-info":[{"award-number":["P30 EY031631"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The purpose of this study was to examine transient plasma membrane disruptions (TPMDs) and TPMD-induced Ca<jats:sup>++<\/jats:sup> waves (TPMD Ca<jats:sup>++<\/jats:sup> Wvs) in human and mouse corneal epithelium (HCEC and MCEC). A multi-photon microscope was used to create laser\u2013induced TPMDs in single cultured cells and in intact <jats:italic>ex vivo<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> MCECs and <jats:italic>ex vivo<\/jats:italic> human cornea rim HCECs. Eye rubbing-induced TPMDs were studied by gentle rubbing with a cotton tipped applicator over a closed eyelid in <jats:italic>ex vivo<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> MCECs. Ca<jats:sup>++<\/jats:sup> sources for TPMD-induced Ca<jats:sup>++<\/jats:sup> waves were explored using Ca<jats:sup>++<\/jats:sup> channel inhibitors and Ca<jats:sup>++<\/jats:sup>-free media. TPMDs and TPMD Ca<jats:sup>++<\/jats:sup> Wvs were observed in all cornea epithelial models examined, often times showing oscillating Ca<jats:sup>++<\/jats:sup> levels. The sarcoplasmic reticulum Ca<jats:sup>++<\/jats:sup> ATPase inhibitors thapsigargin and CPA reduced TPMD Ca<jats:sup>++<\/jats:sup> Wvs. TRP V1 antagonists reduced TPMD Ca<jats:sup>++<\/jats:sup> Wvs in MCECs but not HCECs. Ca<jats:sup>++<\/jats:sup>-free medium, 18\u03b1-GA (gap junction inhibitor), apyrase (hydrolyzes ATP), and AMTB (TRPM8 inhibitor) did not affect TPMD Ca<jats:sup>++<\/jats:sup> Wvs. These results provide a direct demonstration of corneal epithelial cell TPMDs and TPMDs in <jats:italic>in vivo<\/jats:italic> cells from a live animal. TPMDs were observed following gentle eye rubbing, a routine corneal epithelial cell mechanical stress, indicating TPMDs and TPMD Ca<jats:sup>++<\/jats:sup> Wvs are common features in corneal epithelial cells that likely play a role in corneal homeostasis and possibly pathophysiological conditions. Intracellular Ca<jats:sup>++<\/jats:sup> stores are the primary Ca<jats:sup>++<\/jats:sup> source for corneal epithelial cell TPMD Ca<jats:sup>++<\/jats:sup> Wvs, with TRPV1 Ca<jats:sup>++<\/jats:sup> channels providing Ca<jats:sup>++<\/jats:sup> in MCECs but not HCECs. Corneal epithelial cell TPMD Ca<jats:sup>++<\/jats:sup> Wv propagation is not influenced by gap junctions or ATP.<\/jats:p>","DOI":"10.1371\/journal.pone.0301495","type":"journal-article","created":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T17:50:30Z","timestamp":1713376230000},"page":"e0301495","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transient plasma membrane disruption induced calcium waves in mouse and human corneal epithelial cells"],"prefix":"10.1371","volume":"19","author":[{"given":"Zhong","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2079-9887","authenticated-orcid":true,"given":"Mitchell 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