{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T05:28:14Z","timestamp":1792301294757,"version":"4.4.0"},"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>In our ongoing project, which focuses on the introgression of <jats:italic>Booroola<\/jats:italic>\/<jats:italic>FecB<\/jats:italic> gene and the <jats:italic>myostatin<\/jats:italic> (<jats:italic>MSTN<\/jats:italic>) gene into purebred Moghani sheep, we assessed the performance of second-generation Moghani crossbreds such as second crossbreds (F2) and initial backcross generation (BC1). These crossbreds were generated through different mating systems, including in-breeding, outcrossing, first paternal backcrossing (PBC1), and first maternal backcrossing (MBC1). Notably, F2 strains exhibited lean tail, woolly fleece and a higher percentage of white coat color compared to BC1. The impact of mating systems and birth types on pre-weaning survival rates was found to be statistically significant (<jats:italic>P<\/jats:italic> &lt; 0.0001), with singleton offspring resulting from paternal backcross showing a particularly substantial effect. The F2 crossbred lambs carrying the <jats:italic>Booroola<\/jats:italic> gene did not show a statistically significant difference in survivability compared to those carrying the <jats:italic>MSTN<\/jats:italic> gene, implying the <jats:italic>Booroola<\/jats:italic> prolificacy gene had no significant impact on survival outcomes. However, the occurrence of multiple births had a significant negative impact on lamb survival (<jats:italic>P<\/jats:italic> &lt; 0.0001). The PBC1 sheep strains, specifically Texel Tamlet ram strains carrying the <jats:italic>MSTN<\/jats:italic> mutation, exhibited superior growth rates compared to others (<jats:italic>P<\/jats:italic> &lt; 0.05). Interestingly, the <jats:italic>MSTN<\/jats:italic> mutation in the homozygous variant genotype significantly impacts growth rate before weaning compared to other genotypes and pure Moghani sheep (<jats:italic>P<\/jats:italic> &lt; 0.05). In conclusion, this study objectively underscores the pivotal role of genetic factors, specifically through strategic mating systems like paternal backcrossing, in enhancing desired traits and growth rates in Moghani sheep, thereby contributing valuable insights to the field of sheep breeding programs.<\/jats:p>","DOI":"10.1371\/journal.pone.0301629","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T17:39:18Z","timestamp":1712252358000},"page":"e0301629","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Assessing the performance of Moghani crossbred lambs derived from different mating systems with Texel and Booroola sheep"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5555-8453","authenticated-orcid":true,"given":"Reza","family":"Talebi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Mardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrshad","family":"Zeinalabedini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrbano","family":"Kazemi Alamouti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Fabre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Reza","family":"Ghaffari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,4,4]]},"reference":[{"key":"pone.0301629.ref001","doi-asserted-by":"crossref","first-page":"859","DOI":"10.2527\/1993.714859x","article-title":"Comparison of Texel- and Suffolk-sired crossbred lambs for survival, growth, and compositional traits","volume":"71","author":"KA Leymaster","year":"1993","journal-title":"J Anim Sci"},{"key":"pone.0301629.ref002","doi-asserted-by":"crossref","first-page":"61","DOI":"10.2527\/1999.77161x","article-title":"The importance of genetic diversity in livestock populations of the future","volume":"77","author":"DR Notter","year":"1999","journal-title":"J Anim Sci"},{"key":"pone.0301629.ref003","first-page":"50","article-title":"Fundamental aspects of crossbreeding of sheep: use of breed diversity to improve efficiency of meat production.","volume":"17","author":"KA Leymaster","year":"2002","journal-title":"Sheep Goat Res J"},{"key":"pone.0301629.ref004","first-page":"3495","article-title":"Comparison of the growth performance between pure Moghani sheep and crosses with Texel or Booroola sheep carrying major genes contributing to muscularity and prolificacy","volume":"34","author":"R Talebi","year":"2023","journal-title":"Anim Biotechnol"},{"key":"pone.0301629.ref005","first-page":"100644","article-title":"Effect of genotype and environment on the productive and survivability traits of lambs under a community-based management system","volume":"13","author":"A Abebe","year":"2023","journal-title":"J Agric Food Res"},{"key":"pone.0301629.ref006","first-page":"1","article-title":"Comparison of the growth performance traits in F1 crossbred lambs between two strains Booroola Merino \u00d7 Moghani and Booroola Romney \u00d7 Moghani. 12th National and 4th","author":"R Talebi","year":"2021","journal-title":"International Biotechnology Congress of Islamic Republic of Iran"},{"key":"pone.0301629.ref007","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.smallrumres.2011.09.036","article-title":"Genetic related effects on sheep meat quality.","volume":"101","author":"DL Hopkins","year":"2011","journal-title":"Small Rumin Res"},{"key":"pone.0301629.ref008","doi-asserted-by":"crossref","first-page":"106090","DOI":"10.1016\/j.smallrumres.2020.106090","article-title":"Invited review: Opportunities for genetic improvement toward higher prolificacy in sheep","volume":"186","author":"E. Gootwine","year":"2020","journal-title":"Small Rumin Res"},{"key":"pone.0301629.ref009","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/age.13016","article-title":"All sheeps and sizes: a genetic investigation of mature body size across sheep breeds reveals a polygenic nature","volume":"52","author":"CJ Posbergh","year":"2021","journal-title":"Anim Genet"},{"key":"pone.0301629.ref010","doi-asserted-by":"crossref","first-page":"107020","DOI":"10.1016\/j.smallrumres.2023.107020","article-title":"Association of ovine follistatin gene polymorphisms with body measurements, fat-tail traits and morphometric of head in Iranian Mehraban sheep.","volume":"225","author":"R Talebi","year":"2023","journal-title":"Small Rumin Res."},{"key":"pone.0301629.ref011","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1111\/age.13266","article-title":"Genetic basis of muscle-related traits in sheep: A review","volume":"53","author":"R Talebi","year":"2022","journal-title":"Anim Genet"},{"key":"pone.0301629.ref012","first-page":"110","article-title":"Search for a locus near to myostatin that increases muscling in Texel sheep in New Zealand.","volume":"60","author":"TE Broad","year":"2000","journal-title":"Proc New Zeal Soc Anim Prod"},{"key":"pone.0301629.ref013","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.2527\/jas.2008-1508","article-title":"Investigations into the GDF8 g+6723G-A polymorphism in New Zealand Texel sheep","volume":"87","author":"PL Johnson","year":"2009","journal-title":"J Anim Sci"},{"key":"pone.0301629.ref014","first-page":"86","article-title":"Finding major gene effects in Australian meat sheep: feasibility study for a Texel dataset","volume":"13","author":"K Marshall","year":"1999","journal-title":"Proc Assoc Adv Anim Breed Genet"},{"key":"pone.0301629.ref015","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/ng1810","article-title":"A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep","volume":"38","author":"A Clop","year":"2006","journal-title":"Nat Genet"},{"issue":"75","key":"pone.0301629.ref016","article-title":"Investigating the role of myostatin in the determinism of double muscling characterizing Belgian Texel sheep","volume":"29","author":"F Marcq","year":"1998","journal-title":"Anim Genet"},{"key":"pone.0301629.ref017","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1111\/j.1365-2052.2009.01855.x","article-title":"A frameshift mutation in the coding region of the myostatin gene (MSTN) affects carcass conformation and fatness in Norwegian White Sheep (Ovis aries).","volume":"40","author":"IA Boman","year":"2009","journal-title":"Anim Genet"},{"key":"pone.0301629.ref018","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1111\/j.1365-2052.2008.01734.x","article-title":"Two single nucleotide polymorphisms in the myostatin (GDF8) gene have significant association with muscle depth of commercial Charollais sheep.","volume":"39","author":"G Hadjipavlou","year":"2008","journal-title":"Anim Genet"},{"key":"pone.0301629.ref019","first-page":"25","article-title":"Marker-assisted introgression of myostatin from Texel to Raml\u0131\u00e7 sheep: Growth and real-time ultrasound carcass traits in F1 and BC1 lambs.","author":"K \u00c7elikeloglu","year":"2021","journal-title":"Ankara \u00dcniversitesi Vet Fak\u00fcltesi Derg."},{"key":"pone.0301629.ref020","doi-asserted-by":"crossref","first-page":"231","DOI":"10.5194\/aab-65-231-2022","article-title":"The comparative results of myostatin introgression from donor Texel to recipient Raml\u0131\u00e7 sheep with the aspects of growth, pre-, and post-slaughter carcass traits in the second backcross generation.","volume":"65","author":"M Tekerli","year":"2022","journal-title":"Arch Anim Breed"},{"key":"pone.0301629.ref021","first-page":"39","article-title":"Eskandari Nasab morad pasha. Marker Assisted Introgression of FecB gene into Afshari Sheep","volume":"39","author":"S Qanbari","year":"2009","journal-title":"Iran J Anim Sci"},{"key":"pone.0301629.ref022","doi-asserted-by":"crossref","first-page":"513","DOI":"10.3923\/biotech.2007.513.519","article-title":"A preliminary study of marker data applicability in gene introgression program for Afshari sheep breed","volume":"6","author":"S Qanbari","year":"2007","journal-title":"Biotechnology"},{"key":"pone.0301629.ref023","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1071\/RD05134","article-title":"Carrying the FecB (Booroola) mutation is associated with lower birth weight and slower post-weaning growth rate for lambs, as well as a lighter mature bodyweight for ewes.","volume":"18","author":"E Gootwine","year":"2006","journal-title":"Reprod Fertil Dev"},{"key":"pone.0301629.ref024","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/S0301-6226(01)00240-8","article-title":"Genetic and economic analysis of introgression the B allele of the FecB (Booroola) gene into the Awassi and Assaf dairy breeds.","volume":"71","author":"E Gootwine","year":"2001","journal-title":"Livest Prod Sci"},{"key":"pone.0301629.ref025","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.anireprosci.2007.09.009","article-title":"Prolificacy and lamb survival at birth in Awassi and Assaf sheep carrying the FecB (Booroola) mutation.","volume":"108","author":"E Gootwine","year":"2008","journal-title":"Anim Reprod Sci"},{"key":"pone.0301629.ref026","first-page":"90","article-title":"Biological and economic consequences of introgression of the FecB (booroola) gene into deccani sheep.","author":"C Nimbkar","year":"2009","journal-title":"Proceedings of the International Booroola Workshop ACIAR Proceedings No 133 Australian Centre for International Agricultural Research, Pune, Maharashtra, India."},{"key":"pone.0301629.ref027","unstructured":"Teyssier J, Bodin L, Maton C, Bouquet PM, Elsen JM. Biological and economic consequences of introgression of the FecB gene into the French M\u00e9rinos d\u2019Arles sheep. In: Walkden-Brown, S.W., van der Werf, J.H.J., Nimbkar, C., Gupta VS, (Eds.) U of the F (Booroola) G in S-BP, editors. Proceedings of ACIAR Proceedinga No 133 Australian Centre for International Agricultural Research, Puna Maharashtra India. Helen Newton Turner Memorial International Workshop.; 2009. pp. 128\u2013134."},{"key":"pone.0301629.ref028","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1007\/s11250-011-9862-9","article-title":"Wool characteristics in the third generation of Arkharmerino \u00d7 Ghezel and Arkharmerino \u00d7 Moghani crossbreed sheep.","volume":"43","author":"H Esfandyari","year":"2011","journal-title":"Trop Anim Health Prod"},{"key":"pone.0301629.ref029","first-page":"149","article-title":"Evaluation of Fleece Characterstics of First and Second Generations of ArkharMerinos\u00d7Ghezel and ArkharMerinos \u00d7 Moghani Crossbred sheep","volume":"15","author":"M Mokhber Yousefabad","year":"2008","journal-title":"J Agric Sci Nat Resour"},{"key":"pone.0301629.ref030","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0301-6226(01)00301-3","article-title":"Ewe productivity of Booroola Merino-Rambouillet crossbred sheep during early stages of the introgression of the Fecb allele into a rambouillet population.","volume":"75","author":"BR Southey","year":"2002","journal-title":"Livest Prod Sci"},{"key":"pone.0301629.ref031","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.smallrumres.2015.12.030","article-title":"Estimation of genetic parameters and genetic trends for biometric traits in Moghani sheep breed.","volume":"134","author":"S Bakhshalizadeh","year":"2016","journal-title":"Small Rumin Res"},{"key":"pone.0301629.ref032","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1017\/S0021859617000326","article-title":"Modelling growth curve in Moghani sheep: Comparison of non-linear mixed growth models and estimation of genetic relationship between growth curve parameters","volume":"155","author":"NG Hossein-Zadeh","year":"2017","journal-title":"J Agric Sci"},{"key":"pone.0301629.ref033","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.smallrumres.2010.03.010","article-title":"(Co)Variance components and genetic parameter estimates for growth traits in Moghani sheep.","volume":"91","author":"M Jafaroghli","year":"2010","journal-title":"Small Rumin Res"},{"key":"pone.0301629.ref034","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.smallrumres.2010.11.001","article-title":"Genetic analysis of ewe productivity traits in Moghani sheep","volume":"96","author":"A Rashidi","year":"2011","journal-title":"Small Rumin Res"},{"key":"pone.0301629.ref035","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1080\/10495398.2019.1684933","article-title":"A handmade DNA extraction kit using laundry powder; insights on simplicity, cost-efficiency, rapidity, safety and the quality of purified DNA","volume":"32","author":"R Talebi","year":"2021","journal-title":"Anim Biotechnol"},{"key":"pone.0301629.ref036","author":"Statistical Analysis System","year":"2001","journal-title":"SAS Institute, NC, USA"},{"key":"pone.0301629.ref037","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/j.1439-0388.2007.00693.x","article-title":"Inbreeding in genome-wide selection","volume":"124","author":"HD Daetwyler","year":"2007","journal-title":"J Anim Breed Genet"},{"key":"pone.0301629.ref038","doi-asserted-by":"crossref","first-page":"106088","DOI":"10.1016\/j.smallrumres.2020.106088","article-title":"The size of fat tail does not have an effect on growth performance and carcass characteristics in Lori-Bakhtiari lambs.","volume":"187","author":"M Khaldari","year":"2020","journal-title":"Small Rumin Res."},{"key":"pone.0301629.ref039","first-page":"51","article-title":"Growth and carcass characteristics of crossbred progeny from lean-tailed and fat-tailed sheep breeds","volume":"37","author":"M Khaldari","year":"2007","journal-title":"S Afr J Anim Sci"},{"key":"pone.0301629.ref040","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1111\/age.13133","article-title":"Trends towards revealing the genetic architecture of sheep tail patterning: Promising genes and investigatory pathways","volume":"52","author":"P Kalds","year":"2021","journal-title":"Anim Genet"},{"key":"pone.0301629.ref041","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12711-022-00753-3","article-title":"Genetics of the phenotypic evolution in sheep: a molecular look at diversity-driving genes","volume":"54","author":"P Kalds","year":"2022","journal-title":"Genet Sel Evol"},{"key":"pone.0301629.ref042","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/BF02236619","article-title":"Factors affecting lamb survival in crossbred sheep.","volume":"12","author":"RC Malik","year":"1980","journal-title":"Trop Anim Health Prod"},{"key":"pone.0301629.ref043","first-page":"100","article-title":"Biological and economic consequences of introgression of the FecB mutation into merino sheep in Australia.","author":"SW Walkden-Brown","year":"2009","journal-title":"Proceedings of Helen Newton Turner Memorial International Workshop ACIAR Procedings No 133."},{"key":"pone.0301629.ref044","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.anireprosci.2012.01.008","article-title":"Genetic improvement of reproductive efficiency of sheep and goats","volume":"130","author":"DR Notter","year":"2012","journal-title":"Anim Reprod Sci"},{"key":"pone.0301629.ref045","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.resp.2011.04.017","article-title":"Humans at high altitude: Hypoxia and fetal growth.","volume":"178","author":"LG Moore","year":"2011","journal-title":"Respir Physiol Neurobiol."},{"key":"pone.0301629.ref046","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2527\/jas1985.6016","article-title":"Effects of Number of Lambs Suckled and Supplemental Protein Source on Lactating Ewe Performance","volume":"60","author":"SC Loerch","year":"1985","journal-title":"J Anim Sci"},{"key":"pone.0301629.ref047","doi-asserted-by":"crossref","first-page":"137","DOI":"10.2527\/tas2017.0015","article-title":"Impact of birth and rearing type, as well as inaccuracy of recording, on pre-weaning lamb phenotypic and genetic merit for live weight","volume":"1","author":"N McHugh","year":"2017","journal-title":"Transl Anim Sci"},{"key":"pone.0301629.ref048","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.anireprosci.2007.03.012","article-title":"Effects of the Booroola (FecB) genotypes on growth performance, ewe\u2019s productivity efficiency and litter size in Garole \u00d7 Malpura sheep.","volume":"105","author":"S Kumar","year":"2008","journal-title":"Anim Reprod Sci"},{"key":"pone.0301629.ref049","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.2527\/2005.8391988x","article-title":"A directed search in the region of GDF8 for quantitative trait loci affecting carcass traits in Texel sheep","volume":"83","author":"PL Johnson","year":"2005","journal-title":"J Anim Sci"},{"key":"pone.0301629.ref050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1297-9686-42-4","article-title":"Impact of two myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian White Sheep (Ovis aries).","volume":"42","author":"IA Boman","year":"2010","journal-title":"Genet Sel Evol"},{"key":"pone.0301629.ref051","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1111\/j.1365-2052.2009.01965.x","article-title":"Polymorphisms in the ovine myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand Romney sheep.","volume":"41","author":"JGH Hickford","year":"2010","journal-title":"Anim Genet"},{"key":"pone.0301629.ref052","doi-asserted-by":"crossref","first-page":"100604","DOI":"10.1016\/j.animal.2022.100604","article-title":"Inbreeding trends and genetic diversity in purebred sheep populations.","volume":"16","author":"P Rafter","year":"2022","journal-title":"Animal."},{"key":"pone.0301629.ref053","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1111\/age.12178","article-title":"Inbreeding depression in livestock species: Review and meta-analysis","volume":"45","author":"G. Leroy","year":"2014","journal-title":"Anim Genet"},{"key":"pone.0301629.ref054","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.smallrumres.2015.03.015","article-title":"gene haplotypes and their association with growth and carcass traits in New Zealand Romney lambs","volume":"127","author":"J Han","year":"2015","journal-title":"Small Rumin Res"},{"key":"pone.0301629.ref055","first-page":"1","article-title":"Effects of intronic SNPs in the myostatin gene on growth and carcass traits in colored polish merino sheep","volume":"11","author":"E Grochowska","year":"2020","journal-title":"Genes (Basel)."},{"key":"pone.0301629.ref056","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1186\/1297-9686-37-S1-S11","article-title":"Major genes affecting ovulation rate in sheep","volume":"37","author":"G. 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