{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T08:16:17Z","timestamp":1787904977382,"version":"build-2784847793"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100021054","name":"Military Operational Medicine Research Program","doi-asserted-by":"publisher","award":["Proposal 19480"],"award-info":[{"award-number":["Proposal 19480"]}],"id":[{"id":"10.13039\/100021054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The role that inhaled particulate matter plays in the development of post-deployment lung disease among US service members deployed to Southwest Asia during the Global War on Terrorism has been difficult to define. There is a persistent gap in data addressing the relationship between relatively short-term (months to a few years) exposures to high levels of particulate matter during deployment and the subsequent development of adverse pulmonary outcomes. Surgical lung biopsies from deployed service members and veterans (DSMs) and non-deployed service members and veterans (NDSMs) who develop lung diseases can be analyzed to potentially identify residual deployment-specific particles and develop associations with pulmonary pathological diagnoses. We examined 52 surgical lung biopsies from 25 DSMs and 27 NDSMs using field emission scanning electron microscopy (FE-SEM) with energy dispersive x-ray spectroscopy (EDS) to identify any between-group differences in the number and composition of retained inorganic particles, then compared the particle analysis results with the original histopathologic diagnoses. We recorded a higher number of total particles in biopsies from DSMs than from NDSMs, and this difference was mainly accounted for by geologic clays (illite, kaolinite), feldspars, quartz\/silica, and titanium-rich silicate mixtures. Biopsies from DSMs deployed to other Southwest Asia regions (SWA-Other) had higher particle counts than those from DSMs primarily deployed to Iraq or Afghanistan, due mainly to illite. Distinct deployment-specific particles were not identified. Particles did not qualitatively associate with country of deployment. The individual diagnoses of the DSMs and NDSMs were not associated with elevated levels of total particles, metals, cerium oxide, or titanium dioxide particles. These results support the examination of particle-related lung disease in DSMs in the context of comparison groups, such as NDSMs, to assist in determining the strength of associations between specific pulmonary pathology diagnoses and deployment-specific inorganic particulate matter exposure.<\/jats:p>","DOI":"10.1371\/journal.pone.0301868","type":"journal-article","created":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T20:13:20Z","timestamp":1712866400000},"page":"e0301868","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Particle analysis of surgical lung biopsies from deployed and non-deployed US service members during the Global War on Terrorism"],"prefix":"10.1371","volume":"19","author":[{"given":"Leslie","family":"Hayden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Lightner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5358-5557","authenticated-orcid":true,"given":"Stacy","family":"Strausborger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teri J.","family":"Franks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora L.","family":"Watson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Lewin-Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,4,11]]},"reference":[{"key":"pone.0301868.ref001","article-title":"Lewin-Smith M.","author":"Defense Manpower Data Center, Monterey CA","year":"2022","journal-title":"Personal communication"},{"key":"pone.0301868.ref002","doi-asserted-by":"crossref","DOI":"10.7249\/RR1928","volume-title":"Examination of recent deployment experience across the services and components.","author":"JW Wenger","year":"2018"},{"key":"pone.0301868.ref003","volume-title":"Long-term health consequences of exposure to burn pits in Iraq and Afghanistan.","author":"Institute of Medicine","year":"2011"},{"issue":"4","key":"pone.0301868.ref004","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.ccm.2012.09.001","article-title":"Military service and lung disease","volume":"33","author":"CR Rose","year":"2012","journal-title":"Clin Chest Med"},{"key":"pone.0301868.ref005","article-title":"Is deployment an \u201cexposure\u201d in military personnel?","author":"MJ Falvo","year":"2014","journal-title":"Occup Med Forum, JOEM."},{"issue":"8","key":"pone.0301868.ref006","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1513\/AnnalsATS.201904-344WS","article-title":"Respiratory health after military service in Southwest Asia and Afghanistan. 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Bates","year":"2015","journal-title":"J Stat Softw."},{"issue":"13","key":"pone.0301868.ref013","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v082.i13","article-title":"lmerTest package: Tests in linear mixed effects models.","volume":"82","author":"A Kuznetsova","year":"2017","journal-title":"J Stat Softw"},{"key":"pone.0301868.ref014","doi-asserted-by":"crossref","first-page":"11073","DOI":"10.1007\/s12517-015-1970-9","article-title":"Sedimentological, mineralogical, and geochemical characterization of sand dunes in Saudi Arabia.","volume":"8","author":"M Benaafi","year":"2015","journal-title":"Arabian J Geosci"},{"key":"pone.0301868.ref015","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1180\/0009855043930133","article-title":"Weathering of the primary rock-forming minerals: processes, products and rates.","volume":"39","author":"MJ Wilson","year":"2004","journal-title":"Clay Minerals."},{"key":"pone.0301868.ref016","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.taap.2018.09.024","article-title":"Elongate mineral particles in the natural environment","volume":"361","author":"ME Gunter","year":"2018","journal-title":"Toxicol Appl Pharmacol"},{"key":"pone.0301868.ref017","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1080\/15376516.2018.1449042","article-title":"Method to characterize inorganic particulates in lung tissue biopsies using field emission scanning electron microscopy","volume":"28","author":"HA Lowers","year":"2018","journal-title":"Toxicol Mech Methods"},{"issue":"D8","key":"pone.0301868.ref018","doi-asserted-by":"crossref","first-page":"9423","DOI":"10.1029\/1998JD200048","article-title":"Incorporation of mineralogical composition into models of the radiative properties of mineral aerosol from UV to IR wavelength","volume":"104","author":"IN Sokolik","year":"1999","journal-title":"J Geophys Res"},{"issue":"1","key":"pone.0301868.ref019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/JOM.0b013e31824327ca","article-title":"Titanium and iron in lung of a soldier with nonspecific interstitial pneumonitis and bronchiolitis after returning from Iraq","volume":"54","author":"AM Szema","year":"2012","journal-title":"J Occup Environ Med"},{"key":"pone.0301868.ref020","first-page":"85","article-title":"Lung particulate burdens of subjects from the Cincinnati, Ohio urban area.","volume":"5","author":"LE Stettler","year":"1991","journal-title":"Scanning Microsc."},{"key":"pone.0301868.ref021","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1097\/JOM.0000000000000119","article-title":"Iraq dust is respirable, sharp, and metal-laden and induces lung inflammation with fibrosis in mice via IL-2 upregulation and depletion of regulatory T cells.","volume":"56","author":"AM Szema","year":"2014","journal-title":"J Occup Environ Med."},{"key":"pone.0301868.ref022","doi-asserted-by":"crossref","first-page":"702","DOI":"10.2110\/jsr.2011.55","article-title":"Constructing sandstone provenance and classification ternary diagrams using an electronic spreadsheet.","volume":"81","author":"K Zahid","year":"2011","journal-title":"J Sed Res"},{"key":"pone.0301868.ref023","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1183\/09031936.05.00002105","article-title":"Long-term clearance from small airways decreases with age","volume":"26","author":"M Svartengren","year":"2005","journal-title":"Eur Respir J"},{"key":"pone.0301868.ref024","doi-asserted-by":"crossref","first-page":"43","DOI":"10.18332\/tpc\/135516","article-title":"E-cigarette and dual product use as an emerging health threat to the US military.","volume":"7","author":"CH Keltner","year":"2021","journal-title":"Tob Prev Cessat."},{"issue":"3\/4","key":"pone.0301868.ref025","doi-asserted-by":"crossref","first-page":"e183","DOI":"10.1093\/milmed\/usy186","article-title":"Deployment and smokeless tobacco use among active-duty service members in the U.S. military.","volume":"184","author":"J Lin","year":"2019","journal-title":"Mil Med"},{"issue":"6","key":"pone.0301868.ref026","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.amepre.2008.07.009","article-title":"Cigarette smoking and military deployment: A prospective evaluation.","volume":"35","author":"B Smith","year":"2008","journal-title":"Am J Prev Med"},{"key":"pone.0301868.ref027","first-page":"149","article-title":"Comparative pathology of silicate pneumoconiosis","volume":"96","author":"C Brambilla","year":"1979","journal-title":"Am J Pathol"},{"key":"pone.0301868.ref028","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1056\/NEJMoa1101388","article-title":"Constrictive bronchiolitis in soldiers returning from Iraq and Afghanistan","volume":"365","author":"MS King","year":"2011","journal-title":"N Engl J Med"},{"key":"pone.0301868.ref029","first-page":"213","volume-title":"Airborne Hazards Related to Deployment.","author":"CH Welsh","year":"2015"},{"key":"pone.0301868.ref030","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1111\/his.14135","article-title":"Non-necrotizing granulomatous pneumonitis and chronic pleuritis in soldiers deployed to Southwest Asia.","volume":"77","author":"J Gordetsky","year":"2020","journal-title":"Histopathology.Available from"},{"key":"pone.0301868.ref031","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.humpath.2022.02.014","article-title":"Small airways and airspace inflammation and injury distinguish lung histopathology in deployed military personnel from healthy and diseased lungs","volume":"124","author":"CR Rose","year":"2022","journal-title":"Human Pathology"},{"key":"pone.0301868.ref032","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1289\/ehp.6042","article-title":"Chronic exposure to high levels of particulate air pollution and small airway remodeling","volume":"111","author":"A Churg","year":"2003","journal-title":"Environ Health Perspect"},{"key":"pone.0301868.ref033","doi-asserted-by":"crossref","first-page":"e209","DOI":"10.1016\/S2542-5196(23)00008-6","article-title":"Global estimates of daily ambient fine particulate matter concentrations and unequal spatiotemporal distribution of population exposure: a machine learning modelling study","volume":"7","author":"W Yu","year":"2023","journal-title":"Lancet Planet Health"},{"issue":"6","key":"pone.0301868.ref034","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1136\/thx.2003.011734","article-title":"Inter-observer variation between pathologists in diffuse parenchymal lung disease","volume":"59","author":"AG Nicholson","year":"2004","journal-title":"Thorax"},{"issue":"3","key":"pone.0301868.ref035","first-page":"405","article-title":"Current hypotheses on the mechanisms of toxicity of ultrafine particles.","volume":"39","author":"K Donaldson","year":"2003","journal-title":"Ann Ist Super Sanit\u00e0"},{"issue":"Pt 2","key":"pone.0301868.ref036","first-page":"681","article-title":"Quantitative analysis of inorganic particulate burden in situ in tissue sections.","author":"JL Abraham","year":"1983","journal-title":"Scan Electron Microsc."}],"container-title":["PLOS 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