{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:35:11Z","timestamp":1787726111556,"version":"build-2784847793"},"reference-count":66,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T00:00:00Z","timestamp":1714089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Centre of Postgraduate Medical Education","award":["501-1-009-12-21\/MG2"],"award-info":[{"award-number":["501-1-009-12-21\/MG2"]}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2017\/27\/B\/NZ5\/01504"],"award-info":[{"award-number":["2017\/27\/B\/NZ5\/01504"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Maria Sklodowska-Curie National Research Institute of Oncology","award":["SN\/GW\/22\/2020"],"award-info":[{"award-number":["SN\/GW\/22\/2020"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>High-risk Human Papillomavirus (HR-HPV) genotypes, specifically HPV16 and HPV18, pose a significant risk for the development of cervical intraepithelial neoplasia and cervical cancer. In the multifaceted cervical microenvironment, consisting of immune cells and diverse microbiota, <jats:italic>Lactobacillus<\/jats:italic> emerges as a pivotal factor, wielding significant influence in both stabilizing and disrupting the microbiome of the reproductive tract. To analyze the distinction between the cervical microbiota and <jats:italic>Lactobacillus<\/jats:italic>-dominant\/non-dominant status of HR-HPV and non-infected healthy women, sixty-nine cervical swab samples were analyzed, included 44 with HR-HPV infection and healthy controls. All samples were recruited from Human Papillomavirus-based cervical cancer screening program and subjected to 16s rRNA sequencing analysis. Alpha and beta diversity analyses reveal no significant differences in the cervical microbiota of HR-HPV-infected women, including 16 and 18 HPV genotypes, and those with squamous intraepithelial lesion (SIL), compared to a control group. In this study we identified significantly lower abundance of <jats:italic>Lactobacillus mucosae<\/jats:italic> in women with HR-HPV infection compared to the control group. Furthermore, changes in bacterial diversity were noted in <jats:italic>Lactobacillus<\/jats:italic> non-dominant (LND) samples compared to <jats:italic>Lactobacillus<\/jats:italic>-dominant (LD) in both HR-HPV-infected and control groups. LND samples in HR-HPV-infected women exhibited a cervical dysbiotic state, characterized by <jats:italic>Lactobacillus<\/jats:italic> deficiency. In turn, the LD HR-HPV group showed an overrepresentation of <jats:italic>Lactobacillus helveticus<\/jats:italic>. In summary, our study highlighted the distinctive roles of <jats:italic>L<\/jats:italic>. <jats:italic>mucosae<\/jats:italic> and <jats:italic>L<\/jats:italic>. <jats:italic>helveticus<\/jats:italic> in HR-HPV infections, signaling a need for further research to demonstrate potential clinical implications of cervical microbiota dysbiosis.<\/jats:p>","DOI":"10.1371\/journal.pone.0302270","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T17:29:23Z","timestamp":1714152563000},"page":"e0302270","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cervical microbiota dysbiosis associated with high-risk Human Papillomavirus infection"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4036-3191","authenticated-orcid":true,"given":"Natalia","family":"Zeber-Lubecka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kulecka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michalina","family":"Dabrowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Baginska-Drabiuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Glowienka-Stodolak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5997-7516","authenticated-orcid":true,"given":"Andrzej","family":"Nowakowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7146-6295","authenticated-orcid":true,"given":"Aneta","family":"Slabuszewska-Jozwiak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u017cena","family":"Bednorz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilona","family":"J\u0119drzejewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Piasecka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Pawelec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elzbieta","family":"Wojciechowska-Lampka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerzy","family":"Ostrowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,4,26]]},"reference":[{"key":"pone.0302270.ref001","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1042\/CS20160786","article-title":"The human papillomavirus replication cycle, and its links to cancer progression: a comprehensive review","volume":"131","author":"SV Graham","year":"2017","journal-title":"Clinical Science"},{"key":"pone.0302270.ref002","doi-asserted-by":"crossref","first-page":"552028","DOI":"10.3389\/fpubh.2020.552028","article-title":"Epidemiology and Burden of Human Papillomavirus and Related Diseases, Molecular Pathogenesis, and Vaccine Evaluation","volume":"8","author":"AJ Kombe Kombe","year":"2021","journal-title":"Front Public Health"},{"key":"pone.0302270.ref003","doi-asserted-by":"crossref","DOI":"10.1177\/17455065211020702","article-title":"Human papillomavirus persistence or clearance after infection in reproductive age. 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