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Sand flies were collected during four periods over the course of a single year, encompassing both dry and rainy seasons, using CDC light traps placed in peridomiciles where dogs were seropositive for visceral leishmaniasis (VL). A total of 762 sand fly specimens, representing 12 species across seven genera, were collected. <jats:italic>Lutzomyia longipalpis<\/jats:italic> was the most abundant species, comprising 57.6% of the collected specimens, followed by <jats:italic>Nyssomyia neivai<\/jats:italic> (19.6%) and <jats:italic>Nyssomyia whitmani<\/jats:italic> (10.5%). Species richness and diversity varied among collection periods, with the highest diversity observed in January 2019. Molecular analysis detected <jats:italic>Leishmania<\/jats:italic> DNA in 12.5% of the sand fly specimens, with <jats:italic>Le<\/jats:italic>. <jats:italic>infantum<\/jats:italic> being the predominant species. Blood meal analysis revealed feeding on multiple vertebrate species, including humans, rats, dogs, and chickens. The presence of <jats:italic>Leishmania<\/jats:italic> DNA in sand flies, and the identification of human blood meals, highlight the potential role of these species in VL transmission. These findings underscore the importance of continued surveillance and control measures to prevent the spread of VL and reduce transmission risk in the region.<\/jats:p>","DOI":"10.1371\/journal.pone.0302567","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T17:40:09Z","timestamp":1716486009000},"page":"e0302567","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Diversity, Leishmania detection, and blood meal sources of sand flies from Iguatama, Minas Gerais, Brazil"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2799-8267","authenticated-orcid":true,"given":"Felipe","family":"Dutra-R\u00eago","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila","family":"Binder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora Cristina","family":"Capucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talita Pereira","family":"Vaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Dilermando","family":"Andrade Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3930-547X","authenticated-orcid":true,"given":"Gilberto","family":"Fontes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9lia Maria Ferreira","family":"Gontijo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,5,23]]},"reference":[{"key":"pone.0302567.ref001","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-72386-0","volume-title":"The Leishmaniases: Old Neglected Tropical Diseases","author":"F Bruschi","year":"2018","edition":"1"},{"key":"pone.0302567.ref002","unstructured":"PAHO. 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