{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T09:45:32Z","timestamp":1756460732943,"version":"3.38.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The present study is the first from Pakistan being reported with an objective to assess performance of Rayto RT-7600Vet hematology analyzer (HA) for Cholistani cattle blood (n = 134), in comparison to the manual hematological methods. The four hematological attributes <jats:italic>viz<\/jats:italic>. total erythrocyte count (TEC), hemoglobin (Hb), packed cell volume (PCV) and platelet count (PLT) were deduced through HA (A) and manual (M) methods. Various statistical tests were implied to ascertain level of interrelationship, accuracy and level of agreement between the two methods. All attributes attained through manual methods had high positive, leptokurtic distribution (having many outliers) except for PLT-M and PCV-M. The coefficient of variation for attributes attained through HA and manual methods ranged from 16\u201324% and from 16\u201359%, respectively. Comparison between the overall results revealed that all the studied attributes, except TEC, were significantly (P\u22640.05) different for both methods. A weak relationship was noticed between the attributes attained through two methods as indicated by weak r-values and adjusted r-square values. The reliability level of estimating Hb and PCV had highest intraclass correlation coefficient value of 0.722 and 0.555 for average measures, respectively. However, accuracy level, as determined through Lin\u2019s concordance correlation coefficient was highest for TEC (0.9504) for both analytical methods. Poor level of agreement, in general, was shown for the two methods of analysis regarding all four hematological attributes through Bland and Altman test. In conclusion, the Rayto RT-7600Vet) may present data having higher skewness, kurtosis, and CV%, however, they are valid for multi-species hematological analysis. Caution must however, be taken in interpreting their results with corrected reference intervals and CV% for each machine and for each tested attribute.<\/jats:p>","DOI":"10.1371\/journal.pone.0302617","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T17:20:53Z","timestamp":1741713653000},"page":"e0302617","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Performance evaluation of Rayto RT-7600Vet hematology analyzer in side-by-side comparison with manual hematological methods for apparently healthy Cholistani cattle blood"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2708-5599","authenticated-orcid":true,"given":"Umer","family":"Farooq","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mushtaq Hussain","family":"Lashari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zia Ur","family":"Rehman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musadiq","family":"Idris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haroon","family":"Rashid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shagufta","family":"Nasreen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farah","family":"Laraib","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rubaisha","family":"Ameer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Chauhdary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iram","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"pone.0302617.ref001","doi-asserted-by":"crossref","DOI":"10.21037\/jlpm-23-32","article-title":"Modern hematology analyzers: beyond the simple blood cells count (with focus on the red blood cells)","volume":"9","author":"M Daves","year":"2024","journal-title":"J Lab Precision Med"},{"issue":"55","key":"pone.0302617.ref002","first-page":"99","article-title":"Disruptive technologies in haematology for POCT market","author":"D Isebe","year":"2021","journal-title":"Readout: Horiba technical reports"},{"issue":"5","key":"pone.0302617.ref003","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s11250-023-03744-8","article-title":"Assessing total erythrocyte count as a potential attribute for estimating hemoglobin in Cholistani cattle","volume":"55","author":"U Farooq","year":"2023","journal-title":"Trop Anim Health Prod"},{"issue":"4","key":"pone.0302617.ref004","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/j.1939-165X.2000.tb00243.x","article-title":"Validation of the Coulter AcT Diff hematology analyzer for analysis of blood of common domestic animals","volume":"29","author":"H Dawson","year":"2000","journal-title":"Vet Clin Pathol"},{"issue":"4","key":"pone.0302617.ref005","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1111\/vcp.12398","article-title":"Evaluation of performance of veterinary in\u2010clinic hematology analyzers","volume":"45","author":"M Rishniw","year":"2016","journal-title":"Vet Clin Pathol"},{"key":"pone.0302617.ref006","article-title":"Manual, semiautomated, and point-of-care testing in hematology","volume":"154","author":"KS Clark","year":"2019","journal-title":"Rodak\u2019s Hematology-E-Book: Clinical principles and applications"},{"issue":"2","key":"pone.0302617.ref007","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1053\/j.tcam.2011.02.001","article-title":"Advances in hematology analyzers","volume":"26","author":"DB DeNicola","year":"2011","journal-title":"Top Comp Anim Med"},{"key":"pone.0302617.ref008","unstructured":"Research DM. Global hematology analyzers and reagents market- Industry trends and forecast to 2029. India: Databridge Market Research, India, 2022; Contract No.: SKU-25130."},{"issue":"1","key":"pone.0302617.ref009","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.1002\/viw2.5","article-title":"The in vitro diagnostics industry in China","volume":"1","author":"H Song","year":"2020","journal-title":"View"},{"issue":"2","key":"pone.0302617.ref010","doi-asserted-by":"crossref","first-page":"75","DOI":"10.17582\/journal.jahp\/2020\/8.2.75.79","article-title":"Erythrocyte, hemoglobin and hematocrit profile of Bali cattle during the various periods of parturition","volume":"8","author":"I Merdana","year":"2020","journal-title":"J Anim Health Prod"},{"issue":"2","key":"pone.0302617.ref011","first-page":"168","volume":"11","author":"MT Sitohang","year":"2022","journal-title":"Total and differential leukocytes of dermatitis dog after supplemented with Trigona honey for 35 days"},{"issue":"3","key":"pone.0302617.ref012","first-page":"501","article-title":"Serum cytokine and haematological profiles of anaemic children aged 6 to 60 Months old in Port-Harcourt, Nigeria","volume":"5","author":"EJ Nwankwo","year":"2020","journal-title":"Int J Sci Healthcare Res"},{"issue":"10","key":"pone.0302617.ref013","first-page":"1830","article-title":"Haematological outcomes in progression of malaria: A cohort study from district Dera Ismail Khan, Pakistan","volume":"70","author":"Z Ullah","year":"2020","journal-title":"J Pak Med Assoc"},{"key":"pone.0302617.ref014","unstructured":"Ahmad S. Hematology analyzers in Pakistan. In: Farooq U, editor.: Scientific Technical Corporation (Pvt) Ltd; 2023."},{"issue":"5","key":"pone.0302617.ref015","doi-asserted-by":"crossref","first-page":"515","DOI":"10.3343\/alm.2022.42.5.515","article-title":"Unreliable automated complete blood count results: causes, recognition, and resolution","volume":"42","author":"G Gulati","year":"2022","journal-title":"Annals Lab Med"},{"issue":"4","key":"pone.0302617.ref016","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1111\/j.1939-165X.2011.00372.x","article-title":"Performance evaluation of the Sysmex poc H\u2010100i VD iff hematology analyzer for analysis of canine, feline, equine, and bovine blood","volume":"40","author":"B Riond","year":"2011","journal-title":"Vet Clin Pathol"},{"issue":"1","key":"pone.0302617.ref017","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1111\/j.1751-553X.2012.01464.x","article-title":"Evaluation of Mindray BC\u20103600 hematology analyzer in a university hospital","volume":"35","author":"G Shu","year":"2013","journal-title":"Int J Lab Hematol"},{"issue":"2","key":"pone.0302617.ref018","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1939-165X.2007.tb00202.x","article-title":"Clinical evaluation of the CA530\u2010VET hematology analyzer for use in veterinary practice","volume":"36","author":"S Roleff","year":"2007","journal-title":"Vet Clin Pathol"},{"issue":"2","key":"pone.0302617.ref019","first-page":"2074","article-title":"Cholistan and Cholistani breed of cattle","volume":"30","author":"U Farooq","year":"2010","journal-title":"Pak Vet J"},{"issue":"5","key":"pone.0302617.ref020","doi-asserted-by":"crossref","first-page":"e0280659","DOI":"10.1371\/journal.pone.0280659","article-title":"Investigating the potential of packed cell volume for deducing hemoglobin: Cholistani camels in perspective","volume":"18","author":"U Farooq","year":"2023","journal-title":"PloS One"},{"issue":"6","key":"pone.0302617.ref021","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1590\/1678-4162-12597","article-title":"Hematochemical profile of Cholistani cattle being reared in Cholistan desert of Pakistan under pastoralism","volume":"74","author":"Z Saeed","year":"2022","journal-title":"Arq Brasil Med Vet Zoo"},{"issue":"2","key":"pone.0302617.ref022","first-page":"85","article-title":"Camel rearing in Cholistan desert of Pakistan","volume":"29","author":"I Ali","year":"2009","journal-title":"Pak Vet J"},{"key":"pone.0302617.ref023","unstructured":"Dokumen. Rayto RT-7600 Auto-hematology analyzer service manual online: Dokumen Tips; 2020 [cited 2024 27\u20133]. Available from: https:\/\/dokumen.tips\/documents\/rt-7600-service-manualpdf.html?page=1."},{"issue":"2","key":"pone.0302617.ref024","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1751-553X.2008.01050.x","article-title":"ICSH guideline for worldwide point\u2010of\u2010care testing in haematology with special reference to the complete blood count","volume":"30","author":"C Briggs","year":"2008","journal-title":"Int J Lab Hematol"},{"issue":"5","key":"pone.0302617.ref025","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1097\/01.scs.0000180007.30115.fa","article-title":"Producing accurate platelet counts for platelet rich plasma: validation of a hematology analyzer and preparation techniques for counting","volume":"16","author":"JE Woodell-May","year":"2005","journal-title":"J Craniofac Surg"},{"issue":"1","key":"pone.0302617.ref026","first-page":"8","article-title":"Comparative study of hemoglobin estimated by Drabkin\u2019s and Sahli\u2019s methods","volume":"38","author":"P Balasubramaniam","year":"1992","journal-title":"J Postgrad Medicine"},{"key":"pone.0302617.ref027","article-title":"Recommended method for the determination of packed cell volume by centrifugation","author":"WH Organization","year":"2000","journal-title":"World Health Organization"},{"key":"pone.0302617.ref028","author":"NC Jain","year":"1986","journal-title":"Schalm\u2019s veterinary hematology: Lea & Febiger"},{"issue":"4","key":"pone.0302617.ref029","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/vcp.12006","article-title":"ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics","volume":"41","author":"KR Friedrichs","year":"2012","journal-title":"Vet Clin Pathol"},{"key":"pone.0302617.ref030","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1590\/S1676-24442014000100004","article-title":"Performance evaluation of the Sysmex\u00ae XE-2100D automated hematology analyzer","volume":"50","author":"TES Maciel","year":"2014","journal-title":"J Bras Patol Med Lab"},{"issue":"3","key":"pone.0302617.ref031","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1111\/vcp.12383","article-title":"Quality requirements for veterinary hematology analyzers in small animals\u2014a survey about veterinary experts\u2032 requirements and objective evaluation of analyzer performance based on a meta\u2010analysis of method validation studies: bench top hematology analyzer","volume":"45","author":"AM Cook","year":"2016","journal-title":"Vet Clin Pathol"},{"key":"pone.0302617.ref032","first-page":"1","article-title":"International council for standardization in haematology (ICSH) recommendations for\" surrogate reference\" method for the packed cell volume","volume":"9","author":"B Bull","year":"2003","journal-title":"Lab Hematol"},{"issue":"9","key":"pone.0302617.ref033","doi-asserted-by":"crossref","first-page":"5580","DOI":"10.3168\/jds.2013-7736","article-title":"Evaluation of an automated in-house hematology analyzer for bovine blood","volume":"97","author":"L Roland","year":"2014","journal-title":"J Dairy Sci"},{"issue":"2","key":"pone.0302617.ref034","first-page":"105","article-title":"Evaluation of the pocH-100iV DIFF hematology analyzer for use in horses and cattle","volume":"78","author":"P Deprez","year":"2009","journal-title":"Vlaams Diergenees Tijds"},{"issue":"4","key":"pone.0302617.ref035","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1111\/ijlh.13042","article-title":"Digital morphology analyzers in hematology: ICSH review and recommendations","volume":"41","author":"A Kratz","year":"2019","journal-title":"Int J Lab Hematol"},{"key":"pone.0302617.ref036","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/ijlh.12503","article-title":"Verification and quality control of routine hematology analyzers","volume":"38","author":"J Vis","year":"2016","journal-title":"Int J Lab Hematol"},{"key":"pone.0302617.ref037","doi-asserted-by":"crossref","unstructured":"International Council for Standardization in Haematology WG, Briggs C, Culp N, Davis B, d\u2019Onofrio G, Zini G, et al. ICSH guidelines for the evaluation of blood cell analysers including those used for differential leucocyte and reticulocyte counting. Int J Lab Hematol. 2014;36(6): 613\u201327.","DOI":"10.1111\/ijlh.12201"},{"issue":"3","key":"pone.0302617.ref038","doi-asserted-by":"crossref","first-page":"290","DOI":"10.17582\/journal.aavs\/2020\/8.3.290.296","article-title":"Clinical picture and haemogram profile associated withTheileria annulata infection in cattle before and after therapeutic intervention","volume":"8","author":"S Yousef","year":"2020","journal-title":"Adv Anim Vet Sci"},{"issue":"1","key":"pone.0302617.ref039","first-page":"31","article-title":"Comparison of performance of digital Hemoglobinometer over automated hematology analyzer for hemoglobin estimation and its user-friendliness among the pregnant women in selected district hospitals of Madhya Pradesh. Indian journal of community medicine","volume":"44","author":"M Toppo","year":"2019","journal-title":"Indian Assoc Prevent Soc Med"},{"issue":"3","key":"pone.0302617.ref040","first-page":"1028","article-title":"Methods for hemoglobin estimation: A review of\" what works","volume":"2","author":"T Srivastava","year":"2014","journal-title":"J Hematol Transfus"},{"key":"pone.0302617.ref041","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1590\/1089-6891v15i41755","article-title":"Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer","volume":"15","author":"AS Borges","year":"2014","journal-title":"Ci\u00eanc Anim Brasil"},{"key":"pone.0302617.ref042","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1007\/s00580-012-1489-3","article-title":"Comparison of the ProCyte Dx analyzer with the ADVIA 2120 and the manual differential for validation of equine and bovine hemograms","volume":"22","author":"F Goldmann","year":"2013","journal-title":"Comp Clin Pathol"},{"issue":"11","key":"pone.0302617.ref043","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1111\/aor.12703","article-title":"Evaluation of four veterinary hematology analyzers for bovine and ovine blood counts for in vitro testing of medical devices","volume":"40","author":"IL Pieper","year":"2016","journal-title":"Artif Organs"},{"issue":"1","key":"pone.0302617.ref044","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/j.1365-2257.2006.00870.x","article-title":"Spurious counts and spurious results on haematology analysers: a review. Part I: platelets","volume":"29","author":"M Zandecki","year":"2007","journal-title":"Int J Lab Hematol"},{"issue":"8","key":"pone.0302617.ref045","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.5858\/arpa.2013-0439-OA","article-title":"Performance evaluation of Samsung LABGEOHC10 hematology analyzer","volume":"138","author":"IJ Park","year":"2014","journal-title":"Arch Pathol Lab Med"},{"issue":"8","key":"pone.0302617.ref046","first-page":"2395","article-title":"Performance Evaluation of NS-hema21t automated hematology analyzer and comparison of the hematological parameters with Sysmex XT1800i","volume":"3","author":"NBH Mostafa","year":"2017","journal-title":"Int J Sci Res"},{"issue":"2","key":"pone.0302617.ref047","doi-asserted-by":"crossref","first-page":"241","DOI":"10.36326\/kjvs\/2014\/v5i24177","article-title":"Cow, sheep and goat hematological parameters: Comparative studies between automated analyzer and manual methods","volume":"5","author":"IE Ibrhim","year":"2014","journal-title":"Kufa J Vet Med Sci"},{"key":"pone.0302617.ref048","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/vcp.13337","article-title":"A comparison study between the Siemens ADVIA 120 and manual method for the differential white blood cell count in goats","volume":"53","author":"IL Oikonomidis","year":"2024","journal-title":"Vet Clin Pathol"},{"issue":"6","key":"pone.0302617.ref049","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1309\/AJCPOJN03GOXZRZY","article-title":"Absolute neutrophil counts from automated hematology instruments are accurate and precise even at very low levels","volume":"137","author":"EK Amundsen","year":"2012","journal-title":"Am Journal Clin Pathol"},{"issue":"4","key":"pone.0302617.ref050","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1939-165X.2008.00085.x","article-title":"Comparative clinical study of canine and feline total blood cell count results with seven in\u2010clinic and two commercial laboratory hematology analyzers","volume":"37","author":"M Becker","year":"2008","journal-title":"Vet Clin Pathol"},{"issue":"3","key":"pone.0302617.ref051","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1097\/POC.0000000000000139","article-title":"Evaluation of 3 point-of-care testing hematology analyzers for white blood count","volume":"16","author":"N Brouwer","year":"2017","journal-title":"Point Care"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0302617","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T17:21:13Z","timestamp":1741713673000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0302617"}},"subtitle":[],"editor":[{"given":"Jeffrey","family":"Chalmers","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,11]]},"references-count":51,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0302617","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,11]]}}}