{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T03:33:49Z","timestamp":1784345629271,"version":"3.55.0"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The incorporation of phytoactive compounds in the management of malarial vectors holds promise for the development of innovative and efficient alternatives. Nevertheless, the molecular and physiological responses that these bioactive substances induce remain underexplored. This present study investigated the toxicity of different concentrations of aqueous and methanol extracts of <jats:italic>Ocimum tenuiflorum<\/jats:italic> against larvae of <jats:italic>Anopheles gambiae<\/jats:italic> (<jats:italic>sensu stricto<\/jats:italic>) and unraveled the possible underlying molecular pathways responsible for the observed physiological effects. FTIR and GCMS analyses of phytoactive compounds in aqueous and methanol crude extracts of <jats:italic>O<\/jats:italic>. <jats:italic>tenuiflorum<\/jats:italic> showed the presence of OH stretching vibration, C = C stretching modes of aromatics and methylene rocking vibration; ring deformation mode with high levels of trans-\u03b2-ocimene, 3,7-dimethyl-1,3,6-octatriene in aqueous extract and 4-methoxy-benzaldehyde, 1,3,5-trimethyl-cyclohexane and o-cymene in methanol extract. The percentage mortality upon exposure to methanol and aqueous extracts of <jats:italic>O<\/jats:italic>. <jats:italic>tenuiflorum<\/jats:italic> were 21.1% and 26.1% at 24 h, 27.8% and 36.1% at 48 h and 36.1% and 45% at 72 h respectively. Using reverse transcription quantitative polymerase chain reaction (RT-qPCR), down-regulation of <jats:italic>ABC transporter<\/jats:italic>, overexpression of <jats:italic>CYP6M2<\/jats:italic>, <jats:italic>Hsp70<\/jats:italic>, and <jats:italic>\u03b1-esterase<\/jats:italic>, coupled with significantly increased levels of SOD, CAT, and GSH, were observed in <jats:italic>An<\/jats:italic>. <jats:italic>gambiae<\/jats:italic> (<jats:italic>s<\/jats:italic>.<jats:italic>s<\/jats:italic>.) exposed to aqueous and methanol extracts of <jats:italic>O<\/jats:italic>. <jats:italic>tenuiflorum<\/jats:italic> as compared to the control. Findings from this study have significant implications for our understanding of how <jats:italic>An<\/jats:italic>. <jats:italic>gambiae<\/jats:italic> (<jats:italic>s<\/jats:italic>.<jats:italic>s<\/jats:italic>.) larvae detoxify phytoactive compounds.<\/jats:p>","DOI":"10.1371\/journal.pone.0302677","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T17:37:35Z","timestamp":1714671455000},"page":"e0302677","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Phytomediated stress modulates antioxidant status, induces overexpression of CYP6M2, Hsp70, \u03b1-esterase, and suppresses the ABC transporter in Anopheles gambiae (sensu stricto) exposed to Ocimum tenuiflorum extracts"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7316-5979","authenticated-orcid":true,"given":"Harun K.","family":"Aremu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8587-9883","authenticated-orcid":true,"given":"Christianah A.","family":"Dare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2857-0745","authenticated-orcid":true,"given":"Idris A.","family":"Adekale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bukunmi D.","family":"Adetunji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dickson A.","family":"Musa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luqmon A.","family":"Azeez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olu I.","family":"Oyewole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"issue":"6217","key":"pone.0302677.ref001","doi-asserted-by":"crossref","first-page":"1258522","DOI":"10.1126\/science.1258522","article-title":"Highly evolvable malaria vectors: The genomes of 16 Anopheles mosquitoes","volume":"347","author":"DE Neafsey","year":"2015","journal-title":"Science"},{"key":"pone.0302677.ref002","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1186\/s12936-023-04623-1","article-title":"Anopheles vector distribution and malaria transmission dynamics in Gb\u00eak\u00ea region, central C\u00f4te d\u2019Ivoire","volume":"22","author":"AA Koffi","year":"2023","journal-title":"Malaria Journal"},{"key":"pone.0302677.ref003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12936-019-2705-2","article-title":"UDP-glycosyltransferase genes and their association and mutations associated with pyrethroid resistance in Anopheles sinensis (Diptera: Culicidae)","volume":"18","author":"Y Zhou","year":"2019","journal-title":"Malaria Journal"},{"issue":"3","key":"pone.0302677.ref004","doi-asserted-by":"crossref","first-page":"94","DOI":"10.3390\/jox11030007","article-title":"The Central Role of Cytochrome P450 in Xenobiotic Metabolism-A Brief Review on a Fascinating Enzyme Family","volume":"11","author":"F Esteves","year":"2021","journal-title":"Journal of Xenobiotics"},{"key":"pone.0302677.ref005","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1007\/s42690-021-00434-6","article-title":"Resistance to temephos in Anopheles stephensi larvae is associated with increased cytochrome P450 and \u03b1-esterase genes overexpression","volume":"41","author":"P Vivekanandhan","year":"2021","journal-title":"International Journal of Tropical Insect Science"},{"key":"pone.0302677.ref006","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.ibmb.2006.02.004","article-title":"Involvement of cytochrome P450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics","volume":"36","author":"JP David","year":"2006","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"pone.0302677.ref007","doi-asserted-by":"crossref","first-page":"104822","DOI":"10.1016\/j.pestbp.2021.104822","article-title":"Transcriptional regulation of xenobiotic detoxification genes in insects\u2014An overview","volume":"174","author":"D Amezian","year":"2021","journal-title":"Pesticide Biochemistry and Physiology"},{"key":"pone.0302677.ref008","doi-asserted-by":"crossref","first-page":"e0005313","DOI":"10.1371\/journal.pntd.0005313","article-title":"Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families","volume":"11","author":"L Traverso","year":"2017","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"pone.0302677.ref009","article-title":"The ABC gene family in arthropods: Comparative genomics and role in insecticide transport and resistance","author":"W Dermauw","year":"2014","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"pone.0302677.ref010","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1186\/1756-3305-7-349","article-title":"ABC transporters are involved in defense against permethrin insecticide in the malaria vector Anopheles stephensi","volume":"7","author":"S Epis","year":"2014","journal-title":"Parasites & Vectors"},{"issue":"2","key":"pone.0302677.ref011","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jinsphys.2009.09.012","article-title":"Heat shock proteins contribute to mosquito dehydration tolerance","volume":"56","author":"JB Benoit","year":"2010","journal-title":"Journal of Insect Physiology"},{"key":"pone.0302677.ref012","article-title":"Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms","author":"VA Ingham","year":"2018","journal-title":"Nature Communications"},{"issue":"7","key":"pone.0302677.ref013","doi-asserted-by":"crossref","first-page":"e0009587","DOI":"10.1371\/journal.pntd.0009587","article-title":"Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus","volume":"15","author":"I Sierra","year":"2021","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"pone.0302677.ref014","doi-asserted-by":"crossref","first-page":"106384","DOI":"10.1016\/j.actatropica.2022.106384","article-title":"Assessment of larvicidal and genotoxic potentials of extracts of Hyptis suaveolens against Culex quinquefasciatus based on enzyme profile and RAPD-PCR assay","volume":"229","author":"HK Aremu","year":"2022","journal-title":"Acta Tropica"},{"issue":"5","key":"pone.0302677.ref015","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.3390\/ijms23052690","article-title":"Plant Secondary Metabolites as Defense Tools against Herbivores for Sustainable Crop Protection","volume":"23","author":"PA Divekar","year":"2022","journal-title":"International Journal of Molecular Sciences"},{"key":"pone.0302677.ref016","doi-asserted-by":"crossref","first-page":"108709","DOI":"10.1016\/j.exppara.2024.108709","article-title":"Chemical composition and mosquitocidal properties of essential oil from Indian indigenous plants Ocimum tenuiflorum L. and Ocimum americanum L. against three vector mosquitoes","volume":"258","author":"K Ragavendran","year":"2024","journal-title":"Exp Parasitol"},{"key":"pone.0302677.ref017","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.indcrop.2018.10.043","article-title":"Ocimum tenuiflorum oil, a potential insecticide against rice weevil with anti-acetylcholinesterase activity","volume":"126","author":"ML Bhavya","year":"2018","journal-title":"Industrial Crops and Products"},{"issue":"3","key":"pone.0302677.ref018","doi-asserted-by":"crossref","first-page":"e0173564","DOI":"10.1371\/journal.pone.0173564","article-title":"Green tea proanthocyanidins cause impairment of hormone-regulated larval development and reproductive fitness via repression of juvenile hormone acid methyltransferase, insulin-like peptide, and cytochrome P450 genes in Anopheles gambiae sensu stricto","volume":"12","author":"JM Muema","year":"2017","journal-title":"PLoS One"},{"issue":"10","key":"pone.0302677.ref019","doi-asserted-by":"crossref","first-page":"3170","DOI":"10.1016\/S0021-9258(19)45228-9","article-title":"The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase","volume":"247","author":"HP Mistral","year":"1972","journal-title":"Journal of Biological Chemistry"},{"issue":"4073","key":"pone.0302677.ref020","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1126\/science.179.4073.588","article-title":"Selenium: Biochemical role as a component of glutathione peroxidase","volume":"179","author":"JT Rotruck","year":"1973","journal-title":"Science"},{"key":"pone.0302677.ref021","first-page":"283","volume-title":"Handbook of methods for oxygen radical research","author":"A Claiborne","year":"1985"},{"issue":"3","key":"pone.0302677.ref022","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1159\/000136485","article-title":"Bromobenzene induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite","volume":"11","author":"DJ Jollow","year":"1974","journal-title":"Pharmacology"},{"key":"pone.0302677.ref023","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212 \u0394\u0394CT method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"pone.0302677.ref024","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.sajb.2023.01.021","article-title":"Biotoxicity of Azadirachta indica-synthesized silver nanoparticles against larvae of Culex quinquefasciatus","volume":"153","author":"HK Aremu","year":"2023","journal-title":"South African Journal of Botany"},{"key":"pone.0302677.ref025","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.exppara.2019.01.013","article-title":"Tissue damage and cytotoxic effects of Tagetes minuta essential oil against Lucilia cuprina","volume":"198","author":"A Chaaban","year":"2019","journal-title":"Experimental Parasitology"},{"issue":"17","key":"pone.0302677.ref026","doi-asserted-by":"crossref","first-page":"3844","DOI":"10.3390\/molecules25173844","article-title":"Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules against Three Medically Challenging Arthropod Vectors","volume":"25","author":"S Karthi","year":"2020","journal-title":"Molecules"},{"key":"pone.0302677.ref027","doi-asserted-by":"crossref","first-page":"84","DOI":"10.4001\/003.026.0084","article-title":"Insecticidal activity of garlic (Allium sativum) and ginger (Zingiber officinale) oils on the cotton leafworm, Spodoptera littoralis (Boisd) (Lepidoptera: Noctuidae)","volume":"26","author":"HM Hamada","year":"2018","journal-title":"African Entomology"},{"key":"pone.0302677.ref028","doi-asserted-by":"crossref","first-page":"41568","DOI":"10.1007\/s11356-020-10137-z","article-title":"Chemical profile, mosquitocidal, and biochemical effects of essential oil and major components of Dysphania ambrosioides against Culex quinquefasciatus Say","volume":"27","author":"AA Almadiy","year":"2020","journal-title":"Environmental Science and Pollution Research"},{"key":"pone.0302677.ref029","doi-asserted-by":"crossref","first-page":"109743","DOI":"10.1016\/j.vetpar.2022.109743","article-title":"Acaricidal and anthelmintic efficacy of Ocimum basilicum essential oil and its major constituents estragole and linalool, with insights on acetylcholinesterase inhibition","volume":"309","author":"D Alimi","year":"2022","journal-title":"Veterinary Parasitology"},{"issue":"1","key":"pone.0302677.ref030","doi-asserted-by":"crossref","first-page":"466","DOI":"10.53550\/EEC.2023.v29i01.069","article-title":"Larvicidal, Ovicidal, Ovipositional deterrent and adulticidal activity of Tagetes erecta and Cymbopogon nardus against Culex quinquefasciatus and their GCMS analysis","volume":"29","author":"N Srivastava","year":"2023","journal-title":"Ecology, Environment & Conservation"},{"issue":"20","key":"pone.0302677.ref031","doi-asserted-by":"crossref","first-page":"18708","DOI":"10.1007\/s11356-020-08391-2","article-title":"Larvicidal and ovicidal activity of carvacrol, p-cymene, and \u03b3-terpinene from Origanum vulgare essential oil against the cotton bollworm, Helicoverpa armigera (H\u00fcbner)","volume":"27","author":"X Gong","year":"2020","journal-title":"Environmental Science and Pollution Research"},{"key":"pone.0302677.ref032","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.pmpp.2017.03.008","article-title":"Larvicidal and acaricidal efficacy of different solvent extracts of Andrographis echioides against blood-sucking parasites","volume":"101","author":"D Mathivanan","year":"2018","journal-title":"Physiological and Molecular Plant Pathology"},{"key":"pone.0302677.ref033","doi-asserted-by":"crossref","first-page":"102688","DOI":"10.1016\/j.parint.2022.102688","article-title":"Antifeedant and larvicidal activity of bioactive compounds isolated from entomopathogenic fungi Penicillium sp. for the control of agricultural and medically important insect pest (Spodoptera litura and Culex quinquefasciatus)","volume":"92","author":"M Arunthirumeni","year":"2023","journal-title":"Parasitology International"},{"issue":"1","key":"pone.0302677.ref034","doi-asserted-by":"crossref","first-page":"289","DOI":"10.22159\/ajpcr.2019.v12i1.27854","article-title":"Analysis of biological activity and gas chromatography-mass spectrometry study of conventional extraction of Vitex negundo Linn. leaves","volume":"12","author":"S Fatema","year":"2019","journal-title":"Asian Journal of Pharmaceutical and Clinical Research"},{"key":"pone.0302677.ref035","first-page":"2172919","article-title":"Larvicidal Evaluation against Aedes aegypti and Antioxidant and Cytotoxic Potential of the Essential Oil of Tridax procumbens L. Leaves","author":"LB Brand\u00e3o","year":"2021","journal-title":"ScientificWorldJournal"},{"issue":"2","key":"pone.0302677.ref036","doi-asserted-by":"crossref","first-page":"e0150008","DOI":"10.1371\/journal.pone.0150008","article-title":"Evaluation of the Activity of the Essential Oil from an Ornamental Flower against Aedes aegypti: Electrophysiology, Molecular Dynamics, and Behavioral Assays","volume":"11","author":"PC Bezerra-Silva","year":"2016","journal-title":"PLoS ONE"},{"issue":"10","key":"pone.0302677.ref037","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1080\/14786419.2010.534991","article-title":"Insecticidal activity of the root bark essential oil of Periploca sepium Bunge and its main component","volume":"26","author":"SS Chu","year":"2012","journal-title":"Natural Product Research"},{"issue":"2","key":"pone.0302677.ref038","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1080\/0972060X.2015.1137240","article-title":"Chemical Composition and Larvicidal Activity of Bunium persicum Essential Oil Against Two Important Mosquitoes Vectors","volume":"19","author":"A Sanei-Dehkordi","year":"2016","journal-title":"Journal of Essential Oil Bearing Plants"},{"issue":"3","key":"pone.0302677.ref039","doi-asserted-by":"crossref","first-page":"291","DOI":"10.16970\/ted.19938","article-title":"Insecticidal activity of weed plants, Euphorbia prostrata and Chenopodiastrum murale against stored grain insect pest Trogoderma granarium Everts, 1898 (Coleoptera: Dermestidae)","volume":"40","author":"K Sultana","year":"2016","journal-title":"Turkish Journal of Entomology"},{"key":"pone.0302677.ref040","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1439-0418.2001.00503.x","article-title":"Effects of plant extracts on fecundity and fertility of mosquitoes","volume":"125","author":"J Muthukrishnan","year":"2001","journal-title":"Journal of Applied Entomology"},{"key":"pone.0302677.ref041","doi-asserted-by":"crossref","first-page":"3485","DOI":"10.1021\/acs.jafc.6b01157","article-title":"Exposure of a dengue vector to tea and its waste: survival, developmental consequences, and significance for pest management","volume":"64","author":"H Dieng","year":"2016","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"pone.0302677.ref042","first-page":"1","volume-title":"Advances in Insect Physiology: Target Receptors in the Control of the Insect Pests: Part II","author":"H. Merzendorfer","year":"2014"},{"issue":"16","key":"pone.0302677.ref043","doi-asserted-by":"crossref","first-page":"6147","DOI":"10.1073\/pnas.1203452109","article-title":"Identification and validation of a gene causing cross-resistance between insecticide classes in Anopheles gambiae from Ghana","volume":"109","author":"SN Mitchell","year":"2012","journal-title":"Proc Natl Acad Sci"},{"key":"pone.0302677.ref044","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1111\/j.1365-313X.2011.04596.x","article-title":"Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation","volume":"67","author":"Y Zhang","year":"2011","journal-title":"The Plant Journal"},{"key":"pone.0302677.ref045","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s00436-011-2280-0","article-title":"Enzymes-based resistant mechanism in pyrethroid resistant and susceptible Aedes aegypti strains from northern Thailand","volume":"109","author":"P Somwang","year":"2011","journal-title":"Parasitology Research"},{"key":"pone.0302677.ref046","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1186\/1756-3305-7-274","article-title":"Genetic basis of pyrethroid resistance in a population of Anopheles arabiensis, the primary malaria vector in Lower Moshi, north-eastern Tanzania","volume":"7","author":"J Matowo","year":"2014","journal-title":"Parasites & Vectors"},{"key":"pone.0302677.ref047","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1186\/s12864-015-2240-7","article-title":"Transcriptional cellular responses in midgut tissue of Aedes aegypti larvae following intoxication with Cry11Aa toxin from Bacillus thuringiensis","volume":"16","author":"PE Canton","year":"2015","journal-title":"BMC Genomics"},{"key":"pone.0302677.ref048","volume-title":"Heat Shock Proteins in Veterinary Medicine and Sciences. Heat Shock Proteins","author":"MH Pereira"},{"key":"pone.0302677.ref049","article-title":"Interaction of pesticides with p-glycoprotein and other ABC proteins: A survey of the possible importance to insecticide, herbicide, and fungicide resistance","author":"DS Buss","year":"2008","journal-title":"Pesticide Biochemistry and Physiology"},{"key":"pone.0302677.ref050","article-title":"ABC Transporters are involved in defense against permethrin insecticide in the malaria vector Anopheles stephensi","author":"V Mastrantonio","year":"2021","journal-title":"Acta Tropica"},{"issue":"2","key":"pone.0302677.ref051","doi-asserted-by":"crossref","first-page":"e0228407","DOI":"10.1371\/journal.pone.0228407","article-title":"Conidiobolus coronatus induces oxidative stress and autophagy response in Galleria mellonella larvae","volume":"15","author":"M Kazek","year":"2020","journal-title":"PLoS ONE"},{"key":"pone.0302677.ref052","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1080\/02757540.2019.1579199","article-title":"The usability of oxidative stress and detoxification biomarkers in Gammarus pulex for ecological risk assessment of textile dye methyl orange","volume":"34","author":"NC Yildirim","year":"2019","journal-title":"Chemical Ecology"},{"issue":"4","key":"pone.0302677.ref053","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1007\/s00128-021-03403-4","article-title":"Bioaccumulation of Silver and Impairment of Vital Organs in Clarias gariepinus from Co-Exposure to Silver Nanoparticles and Cow Dung Contamination","volume":"108","author":"LA Azeez","year":"2022","journal-title":"Bulletin of Environmental Contamination and Toxicology"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0302677","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T17:38:10Z","timestamp":1714671490000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0302677"}},"subtitle":[],"editor":[{"given":"Mozaniel Santana","family":"de Oliveira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,5,2]]},"references-count":53,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,5,2]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0302677","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,2]]}}}