{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T23:46:10Z","timestamp":1774655170436,"version":"3.50.1"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Birling Laboratories","award":["The funder provided support in the form of salaries for authors SW, AP and TH, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the \u2018author contributions\u2019 section."],"award-info":[{"award-number":["The funder provided support in the form of salaries for authors SW, AP and TH, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the \u2018author contributions\u2019 section."]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Campylobacter hepaticus<\/jats:italic>, the causative agent of Spotty Liver Disease (SLD) is an important disease in cage-free egg producing chickens causing mortality and production drops. <jats:italic>C<\/jats:italic>. <jats:italic>hepaticus<\/jats:italic> is a slow growing <jats:italic>Campylobacter<\/jats:italic> easily overgrown by fecal bacteria. It is currently only reliably isolatable from bile samples. A selective media for isolation from feces or environment would assist diagnosis and impact assessment. Growth of five Australian <jats:italic>C<\/jats:italic>. <jats:italic>hepaticus<\/jats:italic> isolates was studied using Horse blood agar (HBA), sheep blood agar (SBA), Bolton, Preston and Brain Heart Infusion (BHI) base media. Blood and\/or bile were added to Bolton, Preston and BHI medias. <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> was used as a positive control. Plates were incubated in duplicate under microaerophilic conditions at 42\u00b0C for 10 days and examined at days 3\u20135 and 7\u201310 of incubation. Each isolate was examined for sensitivity to 14 antimicrobials using HBA sensitivity plates. Growth was inhibited by BHI and by added bile, while blood improved growth. Further replicates using SBA, HBA, Bolton and Preston media showed best growth on Bolton agar with blood. All five <jats:italic>C<\/jats:italic>. <jats:italic>hepaticus<\/jats:italic> isolates were resistant to trimethoprim and vancomycin, while four were also resistant to rifampicin and bacitracin. Media based upon Bolton plus blood supplemented with vancomycin and trimethoprim might be used as the most appropriate media for selective growth of <jats:italic>C<\/jats:italic>. <jats:italic>hepaticus<\/jats:italic>. The addition of bile to media for <jats:italic>C<\/jats:italic>. <jats:italic>hepaticus<\/jats:italic> isolation and growth will inhibit growth and is not advised.<\/jats:p>","DOI":"10.1371\/journal.pone.0302861","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T18:46:42Z","timestamp":1717181202000},"page":"e0302861","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Developing a selective culturing approach for Campylobacter hepaticus"],"prefix":"10.1371","volume":"19","author":[{"given":"Sheaaz G. J.","family":"Sakur","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah L.","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8830-1806","authenticated-orcid":true,"given":"Anthony","family":"Pavic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanshuo K.","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taha","family":"Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Kotiw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy Isabelle","family":"Muir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8984-3156","authenticated-orcid":true,"given":"Peter John","family":"Groves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"pone.0302861.ref001","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.vetmic.2015.06.008","article-title":"Isolation of a novel thermophilic Campylobacter from cases of spotty liver disease in laying hens and experimental reproduction of infection and microscopic pathology","volume":"179","author":"T. 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