{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T08:26:49Z","timestamp":1780734409675,"version":"3.54.1"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006021","name":"Direction G\u00e9n\u00e9rale de l\u2019Armement","doi-asserted-by":"publisher","award":["Grant no PDH-2-NRBC-2-B-2201"],"award-info":[{"award-number":["Grant no PDH-2-NRBC-2-B-2201"]}],"id":[{"id":"10.13039\/501100006021","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Insecticide resistance in mosquitoes is spreading worldwide and represents a growing threat to vector control. Insecticide resistance is caused by different mechanisms including higher metabolic detoxication, target-site modification, reduced penetration and behavioral changes that are not easily detectable with simple diagnostic methods. Indeed, most molecular resistance diagnostic tools are costly and labor intensive and then difficult to use for routine monitoring of insecticide resistance. The present study aims to determine whether mosquito susceptibility status against the pyrethroid insecticides (mostly used for mosquito control) could be established by the protein signatures of legs and\/or thoraxes submitted to MALDI-TOF Mass Spectrometry (MS). The quality of MS spectra for both body parts was controlled to avoid any bias due to unconformity protein profiling. The comparison of MS profiles from three inbreeds <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> lines from French Guiana (IRF, IR03, IR13), with distinct deltamethrin resistance genotype \/ phenotype and the susceptible reference laboratory line BORA (French Polynesia), showed different protein signatures. On both body parts, the analysis of whole protein profiles revealed a singularity of BORA line compared to the three inbreeding lines from French Guiana origin, suggesting that the first criteria of differentiation is the geographical origin and\/or the breeding history rather than the insecticide susceptibility profile. However, a deeper analysis of the protein profiles allowed to identify 10 and 11 discriminating peaks from leg and thorax spectra, respectively. Among them, a specific peak around 4870 Da was detected in legs and thoraxes of pyrethroid resistant lines compared to the susceptible counterparts hence suggesting that MS profiling may be promising to rapidly distinguish resistant and susceptible phenotypes. Further work is needed to confirm the nature of this peak as a deltamethrin resistant marker and to validate the routine use of MS profiling to track insecticide resistance in <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> field populations.<\/jats:p>","DOI":"10.1371\/journal.pone.0303027","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T17:35:22Z","timestamp":1715362522000},"page":"e0303027","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Potential of MALDI-TOF MS biotyping to detect deltamethrin resistance in the dengue vector Aedes aegypti"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0490-5774","authenticated-orcid":true,"given":"Lionel","family":"Almeras","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique 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the vectors of human infectious diseases.","author":"J Sevestre","year":"2021","journal-title":"Future Microbiol."},{"issue":"2","key":"pone.0303027.ref019","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1128\/JCM.01855-14","article-title":"Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry and molecular biology techniques for identification of Culicoides (Diptera: ceratopogonidae) biting midges in senegal.","volume":"53","author":"M Sambou","year":"2015","journal-title":"J Clin Microbiol"},{"issue":"7","key":"pone.0303027.ref020","doi-asserted-by":"crossref","first-page":"e0005762","DOI":"10.1371\/journal.pntd.0005762","article-title":"Molecular and MALDI-TOF identification of ticks and tick-associated bacteria in Mali.","volume":"11","author":"AZ Diarra","year":"2017","journal-title":"PLoS Negl Trop 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biochemical studies.","volume":"16","author":"Y Epelboin","year":"2021","journal-title":"PloS One."},{"key":"pone.0303027.ref030","author":"World Health Organization","year":"2022","journal-title":"Manual for monitoring insecticide resistance in mosquito vectors and selecting appropriate interventions [Internet]."},{"key":"pone.0303027.ref031","volume-title":"A large genomic duplication spanning multiple P450s contributes to pyrethroid resistance in the dengue mosquito Aedes aegypti [Internet]","author":"T Bacot","year":"2022"},{"issue":"9","key":"pone.0303027.ref032","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1101\/gr.189225.115","article-title":"Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing","volume":"25","author":"F Faucon","year":"2015","journal-title":"Genome Res"},{"issue":"4","key":"pone.0303027.ref033","doi-asserted-by":"crossref","first-page":"e0005526","DOI":"10.1371\/journal.pntd.0005526","article-title":"In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach.","volume":"11","author":"F Faucon","year":"2017","journal-title":"PLoS Negl Trop Dis."},{"issue":"12","key":"pone.0303027.ref034","doi-asserted-by":"crossref","first-page":"e0006093","DOI":"10.1371\/journal.pntd.0006093","article-title":"Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes.","volume":"11","author":"D Tahir","year":"2017","journal-title":"PLoS Negl Trop Dis."},{"issue":"1","key":"pone.0303027.ref035","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s13071-019-3309-y","article-title":"Presence of the point mutations Val1016Gly in the voltage-gated sodium channel detected in a single mosquito from Panama.","volume":"12","author":"O Murcia","year":"2019","journal-title":"Parasit Vectors."},{"issue":"1","key":"pone.0303027.ref036","doi-asserted-by":"crossref","first-page":"e0004351","DOI":"10.1371\/journal.pntd.0004351","article-title":"Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS.","volume":"10","author":"I Lafri","year":"2016","journal-title":"PLoS Negl Trop Dis."},{"issue":"3","key":"pone.0303027.ref037","doi-asserted-by":"crossref","first-page":"168","DOI":"10.3390\/tropicalmed8030168","article-title":"Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling","volume":"8","author":"MM Costa","year":"2023","journal-title":"Trop Med Infect Dis"},{"issue":"1","key":"pone.0303027.ref038","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"JR Landis","year":"1977","journal-title":"Biometrics"},{"key":"pone.0303027.ref039","doi-asserted-by":"crossref","first-page":"113991","DOI":"10.1016\/j.jviromet.2020.113991","article-title":"A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs","volume":"286","author":"MF Rocca","year":"2020","journal-title":"J Virol Methods"},{"issue":"7","key":"pone.0303027.ref040","doi-asserted-by":"crossref","first-page":"e68318","DOI":"10.1371\/journal.pone.0068318","article-title":"Altered protein networks and cellular pathways in severe west nile disease in mice.","volume":"8","author":"C Fraisier","year":"2013","journal-title":"PloS One"},{"issue":"1","key":"pone.0303027.ref041","doi-asserted-by":"crossref","first-page":"e0011033","DOI":"10.1371\/journal.pntd.0011033","article-title":"Impact of the V410L kdr mutation and co-occurring genotypes at kdr sites 1016 and 1534 in the VGSC on the probability of survival of the mosquito Aedes aegypti (L.) to Permanone in Harris County, TX, USA.","volume":"17","author":"JR Hernandez","year":"2023","journal-title":"PLoS Negl Trop Dis."},{"key":"pone.0303027.ref042","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/1756-3305-7-25","article-title":"Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations.","volume":"7","author":"JGB Linss","year":"2014","journal-title":"Parasit Vectors."},{"issue":"2","key":"pone.0303027.ref043","doi-asserted-by":"crossref","first-page":"e30989","DOI":"10.1371\/journal.pone.0030989","article-title":"Insecticide resistance in the dengue vector Aedes aegypti from Martinique: distribution, mechanisms and relations with environmental factors.","volume":"7","author":"S Marcombe","year":"2012","journal-title":"PloS 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Laroche","year":"2017","journal-title":"Malar J."},{"key":"pone.0303027.ref047","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1051\/parasite\/2018041","article-title":"Blood meal identification in the cryptic species Anopheles gambiae and Anopheles coluzzii using MALDI-TOF MS","volume":"25","author":"F Tandina","year":"2018","journal-title":"Parasite Paris Fr"},{"key":"pone.0303027.ref048","first-page":"100735","article-title":"Identification of Algerian field-caught mosquito vectors by MALDI-TOF MS","volume":"31","author":"Z Abdellahoum","year":"2022","journal-title":"Vet Parasitol Reg Stud Rep"},{"issue":"1","key":"pone.0303027.ref049","doi-asserted-by":"crossref","first-page":"11379","DOI":"10.1038\/s41598-020-68272-z","article-title":"Prediction of malaria transmission drivers in Anopheles mosquitoes using artificial intelligence coupled to MALDI-TOF mass spectrometry","volume":"10","author":"C Nabet","year":"2020","journal-title":"Sci Rep"},{"issue":"9","key":"pone.0303027.ref050","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1074\/mcp.R112.018556","article-title":"Peptide identification by tandem mass spectrometry with alternate fragmentation modes","volume":"11","author":"A Guthals","year":"2012","journal-title":"Mol Cell Proteomics MCP"},{"issue":"4","key":"pone.0303027.ref051","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.jasms.2005.12.007","article-title":"Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS\/MS mass spectrometry","volume":"17","author":"X Zhang","year":"2006","journal-title":"J Am Soc Mass Spectrom"},{"issue":"2","key":"pone.0303027.ref052","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1006\/bbrc.2001.4814","article-title":"Molecular analysis of endogenous retrovirus HRES-1: identification of frameshift mutations in region encoding putative 28-kDa autoantigen","volume":"283","author":"D Lefranc","year":"2001","journal-title":"Biochem 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