{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:03Z","timestamp":1789430403385,"version":"build-2803163510"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Applied Research Project of Public Welfare of Huzhou Bureau of Science and Technology","award":["2022GYB10"],"award-info":[{"award-number":["2022GYB10"]}]},{"name":"Huzhou Center for Disease Control and Prevention key discipline: acute Infectious Diseases","award":["ZDXK202202"],"award-info":[{"award-number":["ZDXK202202"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Purpose<\/jats:title>\n<jats:p>The resurgence of pertussis has occurred around the world. However, the epidemiological profiles of pertussis cannot be well understood by current diseases surveillance. This study was designed to understand the seroepidemiological characteristics of pertussis infection in the general population of Huzhou City, evaluate the prevalence infection of pertussis in the population, and offer insights to inform adjustments in pertussis prevention and control strategies.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>From September to October 2023, a cross-sectional serosurvey was conducted in Huzhou City, involving 1015 permanent residents. Serum samples were collected from the study subjects, and pertussis toxin IgG antibodies (Anti-PT-IgG) were quantitatively measured using enzyme-linked immunosorbent assay (ELISA). The analysis included the geometric mean concentration (GMC) of Anti-PT-IgG, rates of GMC\u226540IU\/mL, \u2265100IU\/mL, and &lt;5IU\/mL. Stratified comparisons were made based on age, vaccination history, and human categories.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Among the 1015 surveyed individuals, the geometric mean concentration (GMC) of Anti-PT-IgG was 10.52 (95% CI: 9.96\u201311.11) IU\/mL, with a recent infection rate of 1.58%, a serum positivity rate of 11.43%, and a proportion with &lt;5IU\/mL of 40.49%. Among 357 children with clear vaccination history, susceptibility decreased with an increasing number of vaccine doses (Z = -6.793, <jats:italic>P<\/jats:italic> &lt; 0.001). The concentration of Anti-PT-IgG exhibited a significant post-vaccination decline over time (Z = -5.143, <jats:italic>P<\/jats:italic> &lt; 0.001). In women of childbearing age, the GMC of Anti-PT-IgG was 7.71 (95% CI: 6.90\u20138.62) IU\/mL, with no significant difference in susceptibility among different age groups (\u03c7<jats:sup>2<\/jats:sup> = 0.545, <jats:italic>P<\/jats:italic> = 0.909). The annual pertussis infection rate in individuals aged \u22653 years was 9321 (95%CI: 3336\u201316039) per 100,000, with peak infection rates in the 20\u201329, 40\u201349, and 5\u20139 age groups at 34363 (95%CI: 6327\u201366918) per 100,000, 22307.72 (95%CI: 1380\u201347442) per 100,000, and 18020(95%CI: 1093\u201337266) per 100,000, respectively.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>In 2023, the actual pertussis infection rate in the population of Huzhou City was relatively high. Vaccine-induced antibodies exhibit a rapid decay, and the estimated serum infection rate increases rapidly from post-school age, peaking in the 20\u201329 age group. It is recommended to enhance pertussis monitoring in adolescents and adults and refine vaccine immunization strategies.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0303508","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T17:37:56Z","timestamp":1716226676000},"page":"e0303508","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Seroepidemiology of pertussis in Huzhou: A population-based, cross-sectional study"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8767-9760","authenticated-orcid":true,"given":"Yan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuda","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofu","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangtao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zizhe","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,5,20]]},"reference":[{"issue":"11","key":"pone.0303508.ref001","doi-asserted-by":"crossref","first-page":"2609","DOI":"10.1080\/21645515.2020.1738168","article-title":"Pertussis in high-risk groups: an overview of the past quarter-century.","volume":"16","author":"VA Jenkins","year":"2020","journal-title":"Hum Vaccin Immunother."},{"issue":"5","key":"pone.0303508.ref002","doi-asserted-by":"crossref","first-page":"e0155965","DOI":"10.1371\/journal.pone.0155965","article-title":"Is Pertussis Infection Neglected in China? Evidence from a Seroepidemiology Survey in Zhejiang, an Eastern Province of China.","volume":"11","author":"H He","year":"2016","journal-title":"PLoS One."},{"issue":"11","key":"pone.0303508.ref003","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.1080\/21645515.2019.1608130","article-title":"Seroepidemiology of pertussis in Hangzhou, China, during 2009\u20132017.","volume":"15","author":"Y Xu","year":"2019","journal-title":"Hum Vaccin Immunother"},{"issue":"2","key":"pone.0303508.ref004","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jinf.2005.10.020","article-title":"The incidence of Bordetella pertussis infections estimated in the population from a combination of serological surveys.","volume":"53","author":"HE de Melker","year":"2006","journal-title":"J Infect"},{"issue":"116\u201329","key":"pone.0303508.ref005","article-title":"What is the true burden of diphtheria, tetanus, pertussis and poliovirus in children aged 3\u201318 years in Asia? A systematic literature review","volume":"117","author":"L Nicholson","year":"2022","journal-title":"Int J Infect Dis"},{"issue":"3","key":"pone.0303508.ref006","first-page":"264","article-title":"Epidemiology of pertussis in China, 2011\u20132017","volume":"24","author":"G Ning","year":"2018","journal-title":"Chinese Journal of Vaccines and Immunization"},{"issue":"4","key":"pone.0303508.ref007","first-page":"638","article-title":"Seroepidemiology of pertussis in healthy population in Ningbo, 2019.","volume":"42","author":"XF Zhao","year":"2021","journal-title":"Zhonghua Liu Xing Bing Xue Za Zhi"},{"issue":"52","key":"pone.0303508.ref008","doi-asserted-by":"crossref","first-page":"7950","DOI":"10.1016\/j.vaccine.2018.11.009","article-title":"A big pertussis outbreak in a primary school with high vaccination coverage in northern China: An evidence of the emerging of the disease in China","volume":"36","author":"H 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study","volume":"39","author":"Z Zhang","year":"2021","journal-title":"Vaccine"},{"issue":"1","key":"pone.0303508.ref024","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jinf.2016.04.004","article-title":"Seroprevalence of pertussis among adults in China where whole cell vaccines have been used for 50 years.","volume":"73","author":"Z Chen","year":"2016","journal-title":"J Infect."},{"issue":"1","key":"pone.0303508.ref025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2334-12-138","article-title":"The seroepidemiology of immunoglobulin G antibodies against pertussis toxin in China: a cross sectional study.","volume":"12","author":"Q Zhang","year":"2012","journal-title":"BMC infectious diseases."},{"issue":"48","key":"pone.0303508.ref026","doi-asserted-by":"crossref","first-page":"6956","DOI":"10.1016\/j.vaccine.2022.10.020","article-title":"The decline in immunity and circulation of pertussis among Chinese population during the COVID-19 pandemic: A 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