{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:13:24Z","timestamp":1780568004859,"version":"3.54.1"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022230","name":"Deanship of Scientific Research, Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100023691","name":"Women University Multan","doi-asserted-by":"publisher","award":["17\/2019-20"],"award-info":[{"award-number":["17\/2019-20"]}],"id":[{"id":"10.13039\/501100023691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Microbes maneuver strategies to become incessant and biofilms perfectly play a role in scaling up virulence to cause long-lasting infections. The present study was designed to assess the use of an eco-friendly formulation of functionalized silver nanoparticles generated from <jats:italic>Mentha longifolia<\/jats:italic> leaf extract (M\u2113E) for the treatment of biofilm-producing microbes. Nanoparticles synthesized using M\u2113E as a reducing agent were optimized at different strengths of AgNO<jats:sub>3<\/jats:sub> (1 mM, 2 mM, 3 mM, and 4 mM). Synthesis <jats:italic>of M<\/jats:italic>. <jats:italic>longifolia<\/jats:italic> silver nanoparticles (M\u2113AgNPs) was observed spectrophotometrically (450 nm) showing that M\u2113AgNPs (4 mM) had the highest absorbance. Various techniques e.g., Fourier transforms Infrared spectroscopy (FTIR), Dynamic light scattering (DLS), zeta potential (ZP), X-ray Diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM) were used to characterize M\u2113AgNPs. In the present study, the Kirby\u2014Bauer method revealed 4mM was the most detrimental conc. of M\u2113AgNPs with MIC and MBC values of 0.62 \u03bcg\/mL and 1.25 \u03bcg\/mL, 0.03 \u03bcg\/mL and 0.078 \u03bcg\/mL, and 0.07 \u03bcg\/mL and 0.15 \u03bcg\/mL against previously isolated and identified clinical strains of <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, and <jats:italic>Staphylococcus aureus<\/jats:italic>, respectively. Moreover, the M\u2113AgNP antibiofilm activity was examined via tissue culture plate (TCP) assay that revealed biofilm inhibition of up to 87.09%, 85.6%, 83.11%, and 75.09% against <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>, <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic>, <jats:italic>K<\/jats:italic>. <jats:italic>pneumonia<\/jats:italic>, and <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic>, respectively. Herbal synthesized silver nanoparticles (M\u2113AgNPs) tend to have excellent antibacterial and antibiofilm properties and are promising for other biomedical applications involving the extrication of irksome biofilms. For our best knowledge, it is the first study on the use of the green-synthesized silver nanoparticle M\u2113AgNP as an antibiofilm agent, suggesting that this material has antibiotic, therapeutic, and industrial applications.<\/jats:p>","DOI":"10.1371\/journal.pone.0303521","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T17:36:17Z","timestamp":1720632977000},"page":"e0303521","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mentha longifolia assisted nanostructures: An approach to obliterate microbial biofilms"],"prefix":"10.1371","volume":"19","author":[{"given":"Mahwish Batool","family":"Kazmi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4284-2436","authenticated-orcid":true,"given":"Hayfa Habes","family":"Almutairi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Andleeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raheela","family":"Jabeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-6496","authenticated-orcid":true,"given":"Ghulam","family":"Mustafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umm-e-","family":"Habiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safdar Abbas","family":"Kazmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah","family":"Naz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najma","family":"Qammar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,7,10]]},"reference":[{"key":"pone.0303521.ref001","doi-asserted-by":"crossref","first-page":"124830","DOI":"10.1016\/j.colsurfa.2020.124830","article-title":"Anti-oxidant, anti-bacterial and anti-biofilm activity of biosynthesized silver nanoparticles using Gracilaria corticata against biofilm producing K. pneumoniae","volume":"600","author":"G. 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