{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T05:04:48Z","timestamp":1784610288283,"version":"3.55.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:00:00Z","timestamp":1717027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Aims<\/jats:title>\n<jats:p>To explore the causal relationship between ulcerative colitis (UC) and male infertility using Mendelian randomization method with single nucleotide polymorphism (SNP) as the instrumental variables.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Genetic loci closely associated with UC were extracted as instrumental variables and male infertility was the outcome variable in pooled data from the gene-wide association study (GWAS),which was derived from European ethnic groups. The UC data(ebi-a-GCST003045) contained a total sample size of 27432 individuals and 110944 SNPs, and the male infertility data(finn-b-N14_MALEINFERT) contained a total sample size of 73479 individuals and 16377329 SNPs. The SNPs highly correlated with UC were screened from ebi-a-GCST003045(P&lt;5\u00d710<jats:sup>\u22128<\/jats:sup> as the screening condition, the linkage disequilibrium coefficient was 0.001,and the width of the linkage disequilibrium area was 10000 kb).SNPs related to male infertility from finn-b-N14_MALEINFERT (the minimum r<jats:sup>2<\/jats:sup>&gt;0.8,replacing the missing SNPs with SNPs with high linkage, and deleting SNPs without substitution sites) were extracted. MR analysis was performed using MR-Egger regression, the weighted median and the inverse-variance weighted (IVW) respectively, and the causal relationship between UC and male infertility was evaluated by OR and 95% CI, and the Egger-intercept method was used to test for horizontal multiplicity, and the sensitivity analysis was performed using \"leave-one-out method\". Finally, we used Bayesian Weighted Mendelian Randomization (BWMR) approach to test the results of MR study.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>A total of 86 SNPs were included as IVs, with OR and 95% CI of 1.095(0.820~1.462)\u30011.059(0.899~1.248)\u30011.125(1.002~1.264) for MR-Egger, the weighted median and IVW results respectively, and P value of less than 0.05 for IVW, indicating that a causal relationship between UC and male infertility was causally related. The results of MR analysis combined with BWMR analysis also showed positive genetic causal relationship between UC and male infertility.MR-Egger regression showed an intercept of -2.21\u00d710<jats:sup>\u22123<\/jats:sup> with a standard error of 0.006 and P = 0.751, there was no horizontal pleiotropy for the IVs of exposure factors. Heterogeneity tests showed no heterogeneity and the results of the \"leave-one-out\" sensitivity analysis were stable.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>There is a causal association between UC and male infertility, which increases the risk of developing male infertility.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0303827","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T17:26:44Z","timestamp":1717090004000},"page":"e0303827","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Causal relationship between ulcerative colitis and male infertility: A two-sample Mendelian randomization study"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3933-8346","authenticated-orcid":true,"given":"Xia","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1089-4194","authenticated-orcid":true,"given":"Qiu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,5,30]]},"reference":[{"issue":"1","key":"pone.0303827.ref001","article-title":"Diagnosis and Treatment of Infertility in Men: AUA\/ASRM Guideline Part I","volume":"205","author":"S N P","year":"2021","journal-title":"The Journal of urology"},{"key":"pone.0303827.ref002","article-title":"Recent advances in understanding and managing male infertility","volume":"8","year":"2019","journal-title":"F1000Research"},{"issue":"01","key":"pone.0303827.ref003","first-page":"133","article-title":"Endocrine and metabolic diseases and male infertility","volume":"32","author":"LUO Lingbo","year":"2023","journal-title":"J ournal of Reproductive Medicine"},{"key":"pone.0303827.ref004","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1097\/MIB.0000000000000259","article-title":"Sexual Dysfunction in Inflammatory Bowel Disease: \u201cDon\u2019t Ask, Don\u2019t Tell\u201d Doesn\u2019t Work","volume":"21","author":"S Friedman","year":"2015","journal-title":"Inflamm Bowel Dis"},{"issue":"9","key":"pone.0303827.ref005","doi-asserted-by":"crossref","first-page":"e344","DOI":"10.1016\/j.crohns.2013.01.018","article-title":"Illness Perceptions in IBD Influence Psychological Status, Sexual Health and Satisfaction, Body Image and Relational Functioning: A Preliminary Exploration Using Structural Equation Modeling","volume":"7","author":"SR Knowles","year":"2013","journal-title":"J Crohns Colitis"},{"key":"pone.0303827.ref006","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/ajg.2015.53","article-title":"Assessment Of Sexual Function Among Inflammatory Bowel Disease Patients","volume":"110","author":"S Mahmood","year":"2015","journal-title":"Am J Gastroenterol"},{"key":"pone.0303827.ref007","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1093\/ecco-jcc\/jjv140","article-title":"Inflammatory Bowel Disease and Sexual Function in Male and Female Patients: An Update on Evidence in the Past Ten Years","volume":"9","author":"G Mantzouranis","year":"2015","journal-title":"J Crohns Colitis"},{"key":"pone.0303827.ref008","article-title":"Prevalence and Associated Factors of Sexual Dysfunction in Patients With Inflammatory Bowel Disease","volume":"13","author":"Z Jinzhi","year":"2022","journal-title":"Frontiers in Endocrinology"},{"issue":"1","key":"pone.0303827.ref009","article-title":"\u2019Mendelian randomization\u2019: can genetic epidemiology contribute to understanding environmental determinants of disease?","volume":"32","author":"G S Davey","year":"2003","journal-title":"International journal of epidemiology"},{"issue":"15","key":"pone.0303827.ref010","article-title":"Mendelian randomization: using genes as instruments for making causal inferences in epidemiology","volume":"27","author":"Ziegler Andreas","year":"2008","journal-title":"Statistics in Medicine"},{"issue":"01","key":"pone.0303827.ref011","first-page":"133","article-title":"Endocrine and metabolic diseases and male infertility","volume":"32","author":"LUO Lingbo","year":"2023","journal-title":"Journal of Reproductive Medicine"},{"key":"pone.0303827.ref012","unstructured":"LUO Yangyang. 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