{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:08:56Z","timestamp":1740146936691,"version":"3.37.3"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H02936"],"award-info":[{"award-number":["21H02936"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"Dach1 is highly expressed in normal podocytes, but this expression rapidly disappears after podocyte injury. To investigate the role of Dach1 in podocytes in vivo<\/jats:italic>, we analyzed global, podocyte-specific, and inducible Dach1<\/jats:italic> knockout mice. Global Dach1 knockout (Dach1<\/jats:italic>-\/-<\/jats:sup>) mice were assessed immediately after birth because they die within a day. The kidneys of Dach1<\/jats:italic>-\/-<\/jats:sup> mice were slightly smaller than those of control mice but maintained a normal structure and normal podocyte phenotypes, including ultrastructure. To study the role of Dach1 in mature podocytes, we generated Dach1<\/jats:italic> knockout mice by mating Dach1<\/jats:italic>fl\/fl<\/jats:italic><\/jats:sup> mice with Nphs1<\/jats:italic>-Cre or ROSA<\/jats:italic>-CreERT2 mice. Due to inefficient Cre recombination, only a small number of podocytes lacked Dach1 staining in these mice. However, all eleven Nphs1<\/jats:italic>-Cre\/Dach1<\/jats:italic>fl\/fl<\/jats:italic><\/jats:sup> mice displayed abnormal albuminuria, and seven (63%) of them developed focal segmental glomerulosclerosis. Among 13 ROSA<\/jats:italic>-CreERT2\/Dach1<\/jats:italic>fl\/fl<\/jats:italic><\/jats:sup> mice, eight (61%) exhibited abnormal albuminuria after treatment with tamoxifen, and five (38%) developed early sclerotic lesions. These results indicate that while Dach1 does not determine the fate of differentiation into podocytes, it is indispensable for maintaining the normal integrity of mature podocytes.<\/jats:p>","DOI":"10.1371\/journal.pone.0303910","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T17:55:52Z","timestamp":1716918952000},"page":"e0303910","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dach1 is essential for maintaining normal mature podocytes"],"prefix":"10.1371","volume":"19","author":[{"given":"Keiko","family":"Tanaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1694-9248","authenticated-orcid":true,"given":"Haruko","family":"Hayasaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9466-4585","authenticated-orcid":true,"given":"Taiji","family":"Matsusaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"issue":"3","key":"pone.0303910.ref001","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1681\/ASN.2017091036","article-title":"Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning","volume":"29","author":"NO Lindstrom","year":"2018","journal-title":"J Am Soc 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