{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:23:35Z","timestamp":1747196615655,"version":"3.40.5"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PAPIIT","award":["IN204421"],"award-info":[{"award-number":["IN204421"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The <jats:italic>bla<\/jats:italic>NDM-1 gene and its variants encode metallo-<jats:italic>beta<\/jats:italic>-lactamases that confer resistance to almost all beta-lactam antibiotics. Genes encoding <jats:italic>bla<\/jats:italic>NDM-1 and its variants can be found in several <jats:italic>Acinetobacter<\/jats:italic> species, and they are usually linked to two different plasmid clades. The plasmids in one of these clades contain a gene encoding a Rep protein of the Rep_3 superfamily. The other clade consists of medium-sized plasmids in which the gene (s) involved in plasmid replication initiation (<jats:italic>rep<\/jats:italic>)have not yet been identified. In the present study, we identified the minimal replication region of a <jats:italic>bla<\/jats:italic>NDM-1-carrying plasmid of <jats:italic>Acinetobacter haemolyticus<\/jats:italic> AN54 (pAhaeAN54e), a member of this second clade. This region of 834 paired bases encodes three small peptides, all of which have roles in plasmid maintenance. The plasmids containing this minimal replication region are closely related; almost all contain <jats:italic>bla<\/jats:italic>NDM genes, and they are found in multiple <jats:italic>Acinetobacter<\/jats:italic> species, including <jats:italic>A<\/jats:italic>. <jats:italic>baumannii<\/jats:italic>. None of these plasmids contain an annotated Rep gene, suggesting that their replication relies on the minimal replication region that they share with the plasmid pAhaeAN54e. These observations suggest that this plasmid lineage plays a crucial role in the dissemination of <jats:italic>the bla<\/jats:italic>NDM-1 gene and its variants.<\/jats:p>","DOI":"10.1371\/journal.pone.0303976","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T20:13:19Z","timestamp":1717186399000},"page":"e0303976","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["A novel replication initiation region encoded in a widespread Acinetobacter plasmid lineage carrying a blaNDM-1 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