{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:11:26Z","timestamp":1792181486617,"version":"4.4.0"},"reference-count":15,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T00:00:00Z","timestamp":1718064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312381\/2021\u20102"],"award-info":[{"award-number":["312381\/2021\u20102"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["PROEX 1294\/2023"],"award-info":[{"award-number":["PROEX 1294\/2023"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Canine Alopecia X is a non-inflammatory hair loss disorder of unknown etiology that predominantly affects German Spitz dogs. Treatment modalities include hormone and\/or melatonin supplementation and low trauma microneedling. Melatonin influences hair growth and pigmentation in several species and presents a low risk of adverse effects when used in dogs with Alopecia X. Photobiomodulation (PBM) is frequently used in human androgenetic alopecia and alopecia areata; despite this, PBM remains unexplored in canine Alopecia X. To address this knowledge gap, sixty dogs of both sexes will be randomly assigned to three groups: (i) melatonin only group (3 mg\/Kg, n = 20); (ii) PBM only group (diode laser, wavelength 660nm, 100mw power, with 3 J\/point, 2 sessions\/week for 3 months, n = 20); (ii) PBM + melatonin group (n = 20). The objective is to determine the potential of PBM alone or in conjunction with melatonin supplementation in promoting hair regrowth (hair density and diameter) by means of dermatoscopy and planimetry over a period of 90 days.<\/jats:p>","DOI":"10.1371\/journal.pone.0304605","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T18:20:10Z","timestamp":1718130010000},"page":"e0304605","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison between melatonin versus melatonin and photobiomodulation versus photobiomodulation in the treatment of Alopecia X in German Spitz dogs: Clinical, randomized, double-blind, parallel, non-inferiority protocol"],"prefix":"10.1371","volume":"19","author":[{"given":"Flaviana","family":"Amado Martins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Almeida da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Ligeiro de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinthya Cosme","family":"Gutierrez Duran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9732-6039","authenticated-orcid":true,"given":"Ovidiu","family":"Constantin Baltatu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Labat Marcos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Carolina","family":"Ratto Tempestini Horliana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4694-7617","authenticated-orcid":true,"given":"Stella","family":"Regina Zamuner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6904-9185","authenticated-orcid":true,"given":"Jose","family":"Ant\u00f4nio Silva J\u00fanior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,6,11]]},"reference":[{"issue":"6","key":"pone.0304605.ref001","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1177\/03009858231170295","article-title":"Canine noninflammatory alopecia: An approach to its classification and a diagnostic aid","volume":"60","author":"MM Welle","year":"2023","journal-title":"Vet Pathol"},{"issue":"3","key":"pone.0304605.ref002","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/j.1365-3164.2012.01049.x","article-title":"Canine noninflammatory alopecia: a comprehensive evaluation of common and distinguishing histological characteristics.","volume":"23","author":"T M\u00fcntener","year":"2012","journal-title":"Vet Dermatol"},{"key":"pone.0304605.ref003","volume-title":"Muller & Kirk\u2019s small animal dermatology","author":"L.A. 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Cerundolo","year":"1999","journal-title":"Practice"},{"issue":"9","key":"pone.0304605.ref012","doi-asserted-by":"crossref","first-page":"505","DOI":"10.3390\/vetsci9090505","article-title":"Critically Appraised Topic on Low-Level Laser Therapy (LLLT) in Dogs: An Advisable Treatment for Skin Diseases?","volume":"9","author":"R Perego","year":"2022","journal-title":"Vet Sci"},{"issue":"4","key":"pone.0304605.ref013","doi-asserted-by":"crossref","first-page":"667","DOI":"10.3390\/ani13040667","article-title":"A Systematic Literature Review of Complementary and Alternative Veterinary Medicine: Laser Therapy.","volume":"13","author":"DL Millis","year":"2023","journal-title":"Animals (Basel)."},{"issue":"1","key":"pone.0304605.ref014","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/vde.12170","article-title":"Efficacy of low-level laser therapy on hair regrowth in dogs with noninflammatory alopecia: a pilot study.","volume":"26","author":"L Olivieri","year":"2015","journal-title":"Vet Dermatol."},{"key":"pone.0304605.ref015","unstructured":"Baptista AB. Evaluation of Microneedling in the Treatment of Alopecia X in German Spitz Dogs. M.Sc. Thesis, Santo Amaro University. 2018. Available from: https:\/\/sucupira.capes.gov.br\/sucupira\/public\/consultas\/coleta\/trabalhoConclusao\/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6601787."}],"updated-by":[{"DOI":"10.1371\/journal.pone.0320388","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000}}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0304605","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T18:20:40Z","timestamp":1718130040000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0304605"}},"subtitle":[],"editor":[{"given":"Michael R.","family":"Hamblin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,6,11]]},"references-count":15,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0304605","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,11]]}}}