{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T13:55:37Z","timestamp":1786456537908,"version":"build-2736575974"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>This study aimed to determine the contribution of titanium prepared platelet-rich fibrin (T-PRF) with open flap debridement (OFD) on clinical, biochemical and radiographic measurements of periodontal regeneration. Twenty periodontitis patients with bilateral intrabony defects and stage III grade A periodontitis were included in the study. A total of 40 defects were randomly selected for OFD alone (control group, n = 20) or combined OFD+ T-PRF (test group, n = 20). Clinical and radiographic parameters (at baseline and nine months after surgery), and growth factor levels in gingival crevicular fluid (at baseline and at two, four, six, and twelve weeks after surgical treatment) were also evaluated. Considering the clinical parameters, alterations in probing pocket depth, gingival marginal level and clinical endpoint in the test regions treated with T-PRF significantly improved (<jats:italic>P<\/jats:italic>&lt;0.05). Fibroblast growth factor-2 and platelet-derived growth factor-BB levels between the two groups in the second and fourth weeks were also significantly different (P&lt;0.05). Furthermore, the receptor activator of nuclear factor \u03baB ligand\/osteoprotegerin ratio between the groups was significantly different in the second, fourth, sixth, and twelfth weeks (P&lt;0.05). The bone-filling rate was also significantly greater in the test group than in the control group (P &lt;0.001). Compared with OFD alone, combining T-PRF with the procedure was more successful with regards to clinical, radiographic, and biochemical measurements of periodontal regeneration.<\/jats:p>","DOI":"10.1371\/journal.pone.0304970","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T17:46:20Z","timestamp":1717695980000},"page":"e0304970","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["The effect of titanium-platelet rich fibrin on periodontal intrabony defects: A randomized controlled split-mouth clinical study"],"prefix":"10.1371","volume":"19","author":[{"given":"Didem","family":"Ozkal Eminoglu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taner","family":"Arabaci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0565-6194","authenticated-orcid":true,"given":"Gurbet Alev","family":"Oztas Sahiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,6,6]]},"reference":[{"issue":"2","key":"pone.0304970.ref001","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1089\/ten.teb.2009.0562","article-title":"Periodontal tissue engineering and regeneration: current approaches and expanding opportunities","volume":"16","author":"F.M. 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A novel surgical approach for the management of soft tissues in regenerative procedures. 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Arabaci","year":"2018","journal-title":"Journal of periodontology"},{"issue":"8","key":"pone.0304970.ref047","article-title":"Third-generation platelet concentrates in periodontal regeneration: gaining ground in the field of regeneration","volume":"14","author":"U. Shirbhate","year":"2022","journal-title":"Cureus"},{"issue":"1","key":"pone.0304970.ref048","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s40824-020-00193-4","article-title":"Mechanical, chemical, structural analysis and comparative release of PDGF-AA from L-PRF, A-PRF and T-PRF-an in vitro study","volume":"24","author":"S. Ravi","year":"2020","journal-title":"Biomaterials Research"},{"key":"pone.0304970.ref049","unstructured":"Cutinha, V. (2018). Efficacy of Autologous Titanium-Prepared Platelet Rich Fibrin (T-PRF) with and Without Demineralized Freeze Dried Bone Allograft (DFDBA) in Treatment of Intrabony Defects:\u201cA Comparative Clinical and Radiographic Study\u201d (Doctoral dissertation, Rajiv Gandhi University of Health Sciences (India))."},{"key":"pone.0304970.ref050","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.tripleo.2009.04.020","article-title":"In vitro effects of Choukroun\u2019s PRF (platelet-rich fibrin) on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures","volume":"108","author":"DM Dohan Ehrenfest","year":"2009","journal-title":"Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology"},{"issue":"1","key":"pone.0304970.ref051","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/jre.12291","article-title":"Downregulation of RANKL and RANKL\/osteoprotegerin ratio in human periodontal ligament cells during their osteogenic differentiation","volume":"51","author":"X. Tang","year":"2016","journal-title":"Journal of Periodontal Research"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0304970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T17:46:50Z","timestamp":1717696010000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0304970"}},"subtitle":[],"editor":[{"given":"Gaetano","family":"Isola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,6,6]]},"references-count":51,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0304970","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,6]]}}}