{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:58:52Z","timestamp":1778903932645,"version":"3.51.4"},"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2022M721329"],"award-info":[{"award-number":["2022M721329"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Type II crustacean hyperglycemic hormone (CHH) neuropeptides play diverse roles in crustaceans. In the hermaphrodite shrimp <jats:italic>Lysmata vittata<\/jats:italic>, two transcripts of type II CHHs (molt-inhibiting hormone\/gonad-inhibiting hormone, MIH\/GIH1 and MIH\/GIH2) were identified by transcriptome sequencing, and MIH\/GIH1 was later named <jats:italic>Lvit<\/jats:italic>-GIH1 for its inhibitory effect on ovarian development. Based on the high similarity of MIH\/GIH2 to <jats:italic>Lvit<\/jats:italic>-GIH1, we named tentatively MIH\/GIH2 as <jats:italic>Lvit-<\/jats:italic>GIH2 and explored the role of <jats:italic>Lvit<\/jats:italic>-GIH2 in ovarian development. The open reading frame (ORF) of <jats:italic>Lvit<\/jats:italic>-<jats:italic>GIH2<\/jats:italic> was 333 bp in length, encoding a precursor consisted of a 32-aa signal peptide and a 78-aa mature peptide, which shared high sequence similarity with the type II subfamily peptides in crustaceans. Notably, <jats:italic>Lvit<\/jats:italic>-<jats:italic>GIH2<\/jats:italic> was widely expressed in multiple tissues. The qRT-PCR findings indicated a rising trend in the expression of <jats:italic>Lvit<\/jats:italic>-<jats:italic>GIH2<\/jats:italic> from the male phase to the euhermaphrodite phase. Both RNA interference and addition of GIH2 recombinant proteins (rGIH2) experiments showed that <jats:italic>Lvit<\/jats:italic>-GIH2 suppressed <jats:italic>Lvit<\/jats:italic>-<jats:italic>Vg<\/jats:italic> expression in hepatopancreas and <jats:italic>Lvit<\/jats:italic>-<jats:italic>VgR<\/jats:italic> expression in ovary. To further investigate the role of <jats:italic>Lvit<\/jats:italic>-GIH2 in ovarian development, the RNA-sequence analysis was performed to examine the changes in ovary after addition of rGIH2. The results showed that the pathways (Cysteine and methionine metabolism, Apoptosis-multiple species, etc.) and the genes (<jats:italic>17bHSD8<\/jats:italic>, <jats:italic>IGFR<\/jats:italic>, <jats:italic>CHH<\/jats:italic>, etc.) related to ovarian development were negatively regulated by rGIH2. In brief, <jats:italic>Lvit<\/jats:italic>-GIH2 might inhibit the ovarian development in <jats:italic>L<\/jats:italic>. <jats:italic>vittata<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0305127","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T17:41:04Z","timestamp":1722534064000},"page":"e0305127","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["A new type II CHH neuropeptide involves ovarian development in the peppermint shrimp, Lysmata vittata"],"prefix":"10.1371","volume":"19","author":[{"given":"Huiling","family":"Lan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3898-5867","authenticated-orcid":true,"given":"Haihui","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,8,1]]},"reference":[{"issue":"5","key":"pone.0305127.ref001","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/BF01928162","article-title":"Crustacean neuropeptides: structures, functions and comparative aspects","volume":"48","author":"R. 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