{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T05:28:48Z","timestamp":1782624528830,"version":"3.54.5"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T00:00:00Z","timestamp":1719187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jilin Province Health Talent Special Project","award":["JLSWSRCZX2023-64"],"award-info":[{"award-number":["JLSWSRCZX2023-64"]}]},{"name":"Technology Capacity Enhancement Program of Health Commission of Jilin Province","award":["2021JC067"],"award-info":[{"award-number":["2021JC067"]}]},{"DOI":"10.13039\/501100013254","name":"National College Students Innovation and Entrepreneurship Training Program","doi-asserted-by":"publisher","award":["S202210199051"],"award-info":[{"award-number":["S202210199051"]}],"id":[{"id":"10.13039\/501100013254","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Objective<\/jats:title>\n<jats:p>This study aimed to evaluate the effects of trilobatin (TLB) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice and further explore the underlying mechanisms from the perspectives of signaling pathway and gut microbiota.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>A mouse model of UC was established using DSS. Trilobatin was administered via oral gavage. Disease severity was assessed based on body weight, disease activity index (DAI), colon length, histological detection, inflammation markers, and colonic mucosal barrier damage. Alternations in the NF-\u03baB and PI3K\/Akt pathways were detected by marker proteins. High-throughput 16S rRNA sequencing was performed to investigate the gut microbiota of mice.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>In the DSS-induced UC mice, TLB (30 \u03bcg\/g) treatment significantly increased the body weight, reduced the DAI score, alleviated colon length shortening, improved histopathological changes in colon tissue, inhibited the secretion and expression of inflammation factors (TNF-\u03b1, IL-1\u03b2, and IL-6), and increased the expression of tight-junction proteins (ZO-1 and occludin). Furthermore, TLB (30 \u03bcg\/g) treatment significantly suppressed the activation of NF-\u03baB pathway and altered the composition and diversity of the gut microbiota, as observed in the variations of the relative abundances of Proteobacteria, Actinobacteriota, and Bacteroidota, in UC mice.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>TLB effectively alleviates DSS-induced UC in mice. Regulation of the NF-\u03baB pathway and gut microbiota contributes to TLB-mediated therapeutic effects. Our study not only identified a novel drug candidate for the treatment of UC, but also enhanced our understanding of the biological functions of TLB.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0305926","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T17:22:21Z","timestamp":1719249741000},"page":"e0305926","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Trilobatin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice via the NF-\u03baB pathway and alterations in gut microbiota"],"prefix":"10.1371","volume":"19","author":[{"given":"Nanbo","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2011-3577","authenticated-orcid":true,"given":"Zhihua","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,6,24]]},"reference":[{"issue":"06","key":"pone.0305926.ref001","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1055\/s-0042-1758047","article-title":"Diagnosis and Monitoring of Ulcerative Colitis","volume":"35","author":"ME Murphy","year":"2022","journal-title":"Clinics In Colon And Rectal Surgery"},{"issue":"10080","key":"pone.0305926.ref002","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1016\/S0140-6736(16)32126-2","article-title":"Ulcerative colitis","volume":"389","author":"R Ungaro","year":"2017","journal-title":"Lancet"},{"issue":"4","key":"pone.0305926.ref003","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.pop.2017.07.010","article-title":"An Update on Inflammatory Bowel Disease","volume":"44","author":"T Sairenji","year":"2017","journal-title":"Primary Care"},{"issue":"06","key":"pone.0305926.ref004","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1055\/s-0042-1758048","article-title":"Medical Treatment Options for Ulcerative Colitis","volume":"35","author":"J Sleiman","year":"2022","journal-title":"Clinics In Colon And Rectal Surgery"},{"issue":"1","key":"pone.0305926.ref005","doi-asserted-by":"crossref","DOI":"10.1038\/s41572-020-0205-x","article-title":"Ulcerative colitis","volume":"6","author":"T Kobayashi","year":"2020","journal-title":"Nature Reviews Disease Primers"},{"issue":"7","key":"pone.0305926.ref006","doi-asserted-by":"crossref","DOI":"10.3390\/nu12071973","article-title":"The Effectiveness of Probiotics in the Treatment of Inflammatory Bowel Disease (IBD)-A Critical Review","volume":"12","author":"D Jakubczyk","year":"2020","journal-title":"Nutrients"},{"issue":"2","key":"pone.0305926.ref007","article-title":"Ulcerative Colitis-Induced Colorectal Carcinoma: A Deleterious Concatenation","volume":"14","author":"AP Rivera","year":"2022","journal-title":"Cureus Journal Of Medical Science"},{"issue":"1","key":"pone.0305926.ref008","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10151-019-1926-2","article-title":"Colorectal cancer in inflammatory bowel disease: review of the evidence","volume":"23","author":"DS Keller","year":"2019","journal-title":"Techniques In Coloproctology"},{"issue":"5","key":"pone.0305926.ref009","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1053\/j.gastro.2020.12.036","article-title":"Prognostic Factors for Advanced Colorectal Neoplasia in Inflammatory Bowel Disease: Systematic Review and Meta-analysis","volume":"160","author":"AM Wijnands","year":"2021","journal-title":"Gastroenterology"},{"issue":"2","key":"pone.0305926.ref010","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2174\/187221310791163071","article-title":"Signaling pathways associated with inflammatory bowel disease","volume":"4","author":"J Wei","year":"2010","journal-title":"Recent patents on inflammation & allergy drug discovery"},{"issue":"5","key":"pone.0305926.ref011","doi-asserted-by":"crossref","first-page":"375","DOI":"10.2174\/138945008784221206","article-title":"Cytokines: From gut inflammation to colorectal cancer","volume":"9","author":"MC Fantini","year":"2008","journal-title":"Current Drug Targets"},{"issue":"10","key":"pone.0305926.ref012","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1016\/j.biocel.2006.03.023","article-title":"NF kappa B pathway: A good signaling paradigm and therapeutic target","volume":"38","author":"V. 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