{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T21:21:18Z","timestamp":1774473678061,"version":"3.50.1"},"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>The shortage of donor corneas limits Bowman layer transplantation for keratoconus. In this study, we evaluate the clinical outcome of porcine anterior lens capsule (xenograft) transplantation in the corneal stroma of a rabbit model as substitute for Bowman layer graft that is used in stromal transplantation for advanced keratoconus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Transplantation of porcine anterior lens capsule in the corneal stroma was performed in four New Zealand white rabbits through the creation of a stromal pocket. Corneal transparency, central corneal thickness, and topographic characteristics of corneas were evaluated at different time points: pre- (0) and post- (7, 14, 21, and 28) operative days. Additionally, at the end of the study histopathological findings were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In comparison to pre-operative day, transplantation of an anterior lens capsule preserved corneal transparency, central corneal thickness, and topographic characteristics remained constant throughout the study period. Histopathological analysis revealed the presence of the anterior lens capsule as a fully integrated lamellar graft without adverse effects in host stroma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The anterior lens capsule may be useful as a graft for intrastromal corneal trasplantation. Similarly to Bowman layer, anterior lens capsule has mechanical characteristics that facilitate corneal transplantation. In post-transplanted corneas the preservation of transparency, as well as the effect on corneal thickness, and topographic characteristics support the possibility of using anterior lens capsule as a substitute for Bowman layer graft.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0306015","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T13:32:24Z","timestamp":1732023144000},"page":"e0306015","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Intrastromal graft of anterior lens capsule. A substitute for Bowman layer graft transplantation for keratoconus"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7355-9402","authenticated-orcid":true,"given":"Carlos A.","family":"Rodr\u00edguez-Barrientos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1417-2981","authenticated-orcid":true,"given":"Amir","family":"Translateur-Grynspan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Zavala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge E.","family":"Valdez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisella","family":"Santaella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Barraquer-Coll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"issue":"4","key":"pone.0306015.ref001","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0039-6257(97)00119-7","article-title":"Keratoconus","volume":"42","author":"YS Rabinowitz","year":"1998","journal-title":"Surv Ophthalmol"},{"issue":"2","key":"pone.0306015.ref002","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s40123-017-0099-1","article-title":"Keratoconus Treatment Algorithm.","volume":"6","author":"KD Andreanos","year":"2017","journal-title":"Ophthalmol Ther"},{"issue":"5","key":"pone.0306015.ref003","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.survophthal.2015.02.004","article-title":"Treatment options for advanced keratoconus: A review.","volume":"60","author":"JS Parker","year":"2015","journal-title":"Surv Ophthalmol"},{"issue":"4","key":"pone.0306015.ref004","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1001\/jamaophthalmol.2013.5841","article-title":"Midstromal isolated Bowman layer graft for reduction of advanced keratoconus. A technique to postpone penetrating or deep anterior lamellar keratoplasty","volume":"132","author":"K Van Dijk","year":"2014","journal-title":"JAMA Ophthalmol"},{"issue":"2","key":"pone.0306015.ref005","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1442-9071.2004.00805.x","article-title":"Morphological changes in keratoconus: pathology or pathogenesis.","volume":"32","author":"T Sherwin","year":"2004","journal-title":"Clin Exp Ophthalmol"},{"key":"pone.0306015.ref006","doi-asserted-by":"crossref","first-page":"433","DOI":"10.2147\/OPTH.S141127","article-title":"Bowman\u2019s layer transplantation: Evidence to date.","volume":"12","author":"B Sharma","year":"2018","journal-title":"Clin Ophthalmol"},{"issue":"12","key":"pone.0306015.ref007","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1097\/ICO.0000000000000905","article-title":"Donor tissue preparation for Bowman layer transplantation","volume":"35","author":"EA Groeneveld-Van Beek","year":"2016","journal-title":"Cornea"},{"issue":"2","key":"pone.0306015.ref008","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1098\/rsob.200360","article-title":"To form and function: On the role of basement membrane mechanics in tissue development, homeostasis and disease.","volume":"11","author":"N Khalilgharibi","year":"2021","journal-title":"Open Biol."},{"issue":"4","key":"pone.0306015.ref009","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1097\/00003226-200007000-00001","article-title":"Bowman\u2019s layer structure and function: critical or dispensable to corneal function? A hypothesis.","volume":"19","author":"HJ Wilson SE","year":"2000","journal-title":"Cornea"},{"issue":"5","key":"pone.0306015.ref010","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1097\/OPX.0b013e3182504346","article-title":"Precise thickness measurements of Bowman\u2019s layer, epithelium, and tear film.","volume":"89","author":"T Schmoll","year":"2012","journal-title":"Optom Vis Sci"},{"issue":"2","key":"pone.0306015.ref011","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.exer.2008.08.002","article-title":"The lens capsule","volume":"88","author":"BP Danysh","year":"2009","journal-title":"Exp Eye Res"},{"issue":"3","key":"pone.0306015.ref012","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0174-173X(85)80013-3","article-title":"Immunochemical Characterization of Type IV Procollagen from Anterior Lens Capsule.","volume":"5","author":"JM Brinker","year":"1985","journal-title":"Collagen Rel Res."},{"issue":"3","key":"pone.0306015.ref013","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1088\/1475-4878\/30\/3\/301","article-title":"The function of the lens capsule in the accommodation of the eye.","volume":"30","author":"EF Fincham","year":"1929","journal-title":"Trans Opt Soc"},{"issue":"7","key":"pone.0306015.ref014","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1097\/j.jcrs.0000000000000543","article-title":"Contribution of Bowman layer to corneal biomechanics.","volume":"47","author":"EA Torres-Netto","year":"2021","journal-title":"J Cataract Refract Surg"},{"issue":"5","key":"pone.0306015.ref015","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.ophtha.2014.12.005","article-title":"Bowman layer transplantation to reduce and stabilize progressive, advanced keratoconus","volume":"122","author":"K Van Dijk","year":"2015","journal-title":"Ophthalmology"},{"issue":"22","key":"pone.0306015.ref016","doi-asserted-by":"crossref","first-page":"3037","DOI":"10.1002\/(SICI)1097-0258(19991130)18:22<3037::AID-SIM243>3.0.CO;2-7","article-title":"The use of MIXED models in the analysis of animal experiments with repeated measures data.","volume":"18","author":"Z Wang","year":"1999","journal-title":"Stat Med."},{"issue":"6","key":"pone.0306015.ref017","doi-asserted-by":"crossref","first-page":"675","DOI":"10.5301\/ejo.5000990","article-title":"Technical feasibility of isolated Bowman layer graft preparation by femtosecond laser: a pilot study.","volume":"27","author":"JS Parker","year":"2017","journal-title":"Eur J Ophthalmol"},{"issue":"6","key":"pone.0306015.ref018","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1007\/s00417-018-3927-7","article-title":"Bowman layer transplantation: 5-year results.","volume":"256","author":"K Van Dijk","year":"2018","journal-title":"Graefe\u2019s Arch Clin Exp Ophthalmol."},{"issue":"40","key":"pone.0306015.ref019","first-page":"1","article-title":"Flattening effect of corneal cross-linking depends on the preoperative severity of keratoconus.","volume":"96","author":"K Kasai","year":"2017","journal-title":"Med (United States)."},{"issue":"8","key":"pone.0306015.ref020","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1016\/j.jcrs.2013.01.045","article-title":"Effective corneal collagen crosslinking in advanced cases of progressive keratoconus.","volume":"39","author":"F Sloot","year":"2013","journal-title":"J Cataract Refract Surg"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0306015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T13:32:33Z","timestamp":1732023153000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0306015"}},"subtitle":[],"editor":[{"given":"Georgios","family":"Labiris","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,11,19]]},"references-count":20,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0306015","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.06.11.598436","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,19]]}}}