{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:12:33Z","timestamp":1785931953914,"version":"3.56.0"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Norwegian Research Fund for General Practice"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Objective<\/jats:title>\n<jats:p>People regularly contact emergency medicine services concerned that they have been exposed to drink spiking, i.e., exposure to drugs without their knowledge or permission. We identified drugs in blood and urine samples from patients suspecting exposure to drink spiking, with special consideration for drugs not reported taken by the patient (unreported drugs).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>From September 2018 to May 2019, we collected blood and urine samples from patients 16 years or older presenting at an emergency clinic in Oslo, Norway, within 48 hours of suspected exposure to drink spiking. We also collected information on ethanol ingestion and drugs taken. Blood samples were analyzed for 20 classical recreational drugs using ultra-high performance liquid chromatography\u2013tandem mass spectrometry (UHPLC-MS\/MS) and an automated enzymatic method for ethanol. Urine samples were analyzed using immunoassay methods and a specific gas chromatography mass spectrometry (GCMS) method for gammahydroxybutyrate (GHB).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>From 100 included patients (median age 24 years, 62 females), we collected 100 blood samples and 72 urine samples. Median time since exposure was 5 hours. Unreported drugs were found in 15 patients. Unreported drugs in the blood samples were clonazepam in 3, methylenedioxymethamphetamine (MDMA) in 3, amphetamine in 2, tetrahydrocannabinol (THC) in 2, tramadol in 1, cocaine in 1, and methamphetamine in 1. Unreported drugs in the urine samples were cocaine in 5, amphetamine in 4, ecstasy in 3, and cannabis in 2. Ethanol was found in 69 patients, all reporting ethanol ingestion. Median blood ethanol concentration was higher in patients with no unreported drugs detected, 1.00\u2030 (interquartile range (IQR) 0\u20131.52) vs. 0\u2030 (IQR 0\u20130.46) (p&lt;0.001). GHB was not detected.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Unreported drugs, possibly used for drink spiking, were found in 15% of patients. Blood ethanol concentration was higher when no unreported drugs were found. GHB was not detected in any patient.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0306191","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T17:21:59Z","timestamp":1720632119000},"page":"e0306191","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Drugs in blood and urine samples from victims of suspected exposure to drink spiking: A prospective observational study from Oslo, Norway"],"prefix":"10.1371","volume":"19","author":[{"given":"Vivian M.","family":"Dalaker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e5vard","family":"Furuhaugen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Brekke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari Asphjell","family":"Bj\u00f8rnaas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Krpo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0781-6865","authenticated-orcid":true,"given":"Elisabeth Leere","family":"\u00d8iestad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1213-392X","authenticated-orcid":true,"given":"Odd Martin","family":"Vallersnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,7,10]]},"reference":[{"key":"pone.0306191.ref001","article-title":"I collapsed in the street after drink spiking","author":"B. Robinson","year":"2019","journal-title":"BBC"},{"key":"pone.0306191.ref002","article-title":"Youtuber Agnetesh (26):\u2013Jeg ble dopet ned.","author":"SO Berntsen","year":"2022","journal-title":"NRK"},{"key":"pone.0306191.ref003","article-title":"Drink or food spiking reported to NSW Police has increased by 86% from 2018 to 2022.","author":"M. Even","year":"2023","journal-title":"Canberra Times"},{"key":"pone.0306191.ref004","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1136\/pgmj.2007.059048","article-title":"What\u2019s being used to spike your drink? Alleged spiked drink cases in inner city London.","volume":"83","author":"SL Greene","year":"2007","journal-title":"Postgrad Med J"},{"key":"pone.0306191.ref005","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1136\/emj.2006.040360","article-title":"A study of patients presenting to an emergency department having had a \"spiked drink\".","volume":"24","author":"H Hughes","year":"2007","journal-title":"Emerg Med J"},{"key":"pone.0306191.ref006","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S1355-0306(05)71646-4","article-title":"Clinical urinalysis of drugs and alcohol in instances of suspected surreptitious administration (\"spiked drinks\").","volume":"45","author":"SP Elliott","year":"2005","journal-title":"Sci Justice."},{"key":"pone.0306191.ref007","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1111\/j.1742-6723.2009.01185.x","article-title":"Prospective study of 101 patients with suspected drink spiking.","volume":"21","author":"P Quigley","year":"2009","journal-title":"Emerg Med Australas"},{"key":"pone.0306191.ref008","first-page":"183","article-title":"A review on the forensic toxicology of global drug-facilitated sexual assaults","volume":"26","author":"K Skov","year":"2022","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"pone.0306191.ref009","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jflm.2017.02.005","article-title":"A global epidemiological perspective on the toxicology of drug-facilitated sexual assault: a systematic review","volume":"47","author":"LJ Anderson","year":"2017","journal-title":"J Forensic Leg Med"},{"key":"pone.0306191.ref010","first-page":"10577","article-title":"Drug-facilitated sexual assaults (DFSA): a serious underestimated issue.","volume":"23","author":"FP Busardo","year":"2019","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"pone.0306191.ref011","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1177\/1524838008320221","article-title":"The involvement of drugs and alcohol in drug-facilitated sexual assault: a systematic review of the evidence","volume":"9","author":"CM Beynon","year":"2008","journal-title":"Trauma Violence Abuse"},{"key":"pone.0306191.ref012","first-page":"47","article-title":"Toxicological screening of drug facilated crime among travelers in Dhaka, Bangladesh.","volume":"6","author":"A Basher","year":"2017","journal-title":"Asia Pac J Med Toxicol"},{"key":"pone.0306191.ref013","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/0278-2626(91)90028-7","article-title":"Scopolamine intoxication as a model of transient global amnesia","volume":"15","author":"A Ardila","year":"1991","journal-title":"Brain Cogn"},{"key":"pone.0306191.ref014","doi-asserted-by":"crossref","first-page":"458","DOI":"10.3109\/15563650.2012.702218","article-title":"The clinical toxicology of gamma-hydroxybutyrate, gamma-butyrolactone and 1,4-butanediol.","volume":"50","author":"LJ Schep","year":"2012","journal-title":"Clin Toxicol."},{"key":"pone.0306191.ref015","doi-asserted-by":"crossref","first-page":"47","DOI":"10.2174\/1570159X13666141210215423","article-title":"GHB pharmacology and toxicology: acute intoxication, concentrations in blood and urine in forensic cases and treatment of the withdrawal syndrome.","volume":"13","author":"FP Busardo","year":"2015","journal-title":"Curr Neuropharmacol."},{"key":"pone.0306191.ref016","doi-asserted-by":"crossref","first-page":"137191","DOI":"10.1016\/j.foodchem.2023.137191","article-title":"Exploiting the potential of fabric phase sorptive extraction for forensic food safety: analysis of food samples in cases of drug facilitated crimes","volume":"432","author":"B Jain","year":"2024","journal-title":"Food Chem"},{"key":"pone.0306191.ref017","article-title":"Statistikkbanken: Population Tsable 01222: Population and changes during the quarter.","author":"Statistics Norway"},{"key":"pone.0306191.ref018","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s13049-016-0268-6","article-title":"Outpatient treatment of acute poisoning by substances of abuse: a prospective observational cohort study","volume":"24","author":"OM Vallersnes","year":"2016","journal-title":"Scand J Trauma Resusc Emerg Med"},{"key":"pone.0306191.ref019","doi-asserted-by":"crossref","first-page":"106939","DOI":"10.1016\/j.vascn.2020.106939","article-title":"Determination of the alcohol biomarker phosphatidylethanol 16:0\/18:1 and 33 compounds from eight different drug classes in whole blood by LC-MS\/MS","volume":"107","author":"B Jorgenrud","year":"2021","journal-title":"J Pharmacol Toxicol Methods"},{"key":"pone.0306191.ref020","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/jat\/29.5.387","article-title":"An automated alcohol dehydrogenase method for ethanol quantification in urine and whole blood","volume":"29","author":"L Kristoffersen","year":"2005","journal-title":"J Anal Toxicol"},{"key":"pone.0306191.ref021","first-page":"885","article-title":"Determination of gamma-hydroxybutyrate (GHB), beta-hydroxybutyrate (BHB), pregabalin, 1,4-butane-diol (1,4BD) and gamma-butyrolactone (GBL) in whole blood and urine samples by UPLC-MSMS.","author":"SR Dahl","year":"2012","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"pone.0306191.ref022","unstructured":"European monitoring centre for drugs and drug addiction (EMCDDA). European drug report 2020: trends and developments. Lisbon, Portugal: EMCDDA; 2020."},{"key":"pone.0306191.ref023","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1080\/15563650.2017.1312002","article-title":"Underestimated impact of novel psychoactive substances: laboratory confirmation of recreational drug toxicity in Oslo, Norway.","volume":"55","author":"OM Vallersnes","year":"2017","journal-title":"Clin Toxicol"},{"key":"pone.0306191.ref024","first-page":"e2021546","article-title":"Fourth generation of synthetic cannabinoid receptor agonists: a summary on the latest insights","volume":"92","author":"S Malaca","year":"2022","journal-title":"Acta Biomed"},{"key":"pone.0306191.ref025","doi-asserted-by":"crossref","first-page":"e2727","DOI":"10.1002\/hup.2727","article-title":"The rise of new psychoactive substances and psychiatric implications: a wide-ranging, multifaceted challenge that needs far-reaching common legislative strategies.","volume":"35","author":"R Rinaldi","year":"2020","journal-title":"Hum Psychopharmacol."},{"key":"pone.0306191.ref026","doi-asserted-by":"crossref","first-page":"247","DOI":"10.2165\/00002018-199106040-00003","article-title":"Benzodiazepine poisoning: clinical and pharmacological considerations and treatment.","volume":"6","author":"P Gaudreault","year":"1991","journal-title":"Drug Saf"},{"key":"pone.0306191.ref027","first-page":"1027","article-title":"Acute poisoning from substance abuse of benzodiazepines.","volume":"140","author":"JA Andersson","year":"2020","journal-title":"Tidsskr Nor Legeforen"},{"key":"pone.0306191.ref028","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.forsciint.2017.09.010","article-title":"Toxicological findings in suspected drug-impaired drivers in Norway: trends during 1990\u20132015.","volume":"280","author":"A Valen","year":"2017","journal-title":"Forensic Sci Int"},{"key":"pone.0306191.ref029","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1080\/15563650.2020.1784914","article-title":"MDMA-related presentations to the emergency departments of the European Drug Emergencies Network plus (Euro-DEN Plus) over the four-year period 2014\u20132017.","volume":"59","author":"R Noseda","year":"2021","journal-title":"Clin Toxicol"},{"key":"pone.0306191.ref030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/02791072.2006.10399822","article-title":"Ecstasy (MDMA), methamphetamine, and date rape (drug-facilitated sexual assault): a consideration of the issues.","volume":"38","author":"KL Jansen","year":"2006","journal-title":"J Psychoactive Drugs"},{"key":"pone.0306191.ref031","doi-asserted-by":"crossref","first-page":"327","DOI":"10.2165\/00003088-200342040-00003","article-title":"Pharmacokinetics and pharmacodynamics of cannabinoids.","volume":"42","author":"F. Grotenhermen","year":"2003","journal-title":"Clin Pharmacokinet"},{"key":"pone.0306191.ref032","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.jflm.2013.05.005","article-title":"Ethanol and drug findings in women consulting a Sexual Assault Center: associations with clinical characteristics and suspicions of drug-facilitated sexual assault.","volume":"20","author":"CT Hagemann","year":"2013","journal-title":"J Forensic Leg Med"},{"key":"pone.0306191.ref033","first-page":"9681","article-title":"New psychoactive substances: concerted efforts and common legislative answers for stemming a growing health hazard.","volume":"23","author":"S. Zaami","year":"2019","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"pone.0306191.ref034","doi-asserted-by":"crossref","first-page":"122740","DOI":"10.1016\/j.talanta.2021.122740","article-title":"In silico prediction, LC-HRMS\/MS analysis, and targeted\/untargeted data-mining workflow for the profiling of phenylfentanyl in vitro metabolites.","volume":"235","author":"A Di Trana","year":"2021","journal-title":"Talanta."},{"key":"pone.0306191.ref035","first-page":"4187","article-title":"Assessment of the stability of exogenous gamma hydroxybutyric acid (GHB) in stored blood and urine specimens.","volume":"19","author":"FP Busardo","year":"2015","journal-title":"Eur Rev Med Pharmacol Sci"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0306191","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T17:22:20Z","timestamp":1720632140000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0306191"}},"subtitle":[],"editor":[{"given":"Heather M.","family":"Barkholtz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,7,10]]},"references-count":35,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0306191","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,10]]}}}