{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T19:23:39Z","timestamp":1772220219956,"version":"3.50.1"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T00:00:00Z","timestamp":1730332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1828164"],"award-info":[{"award-number":["1828164"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007656","name":"Loyola University Chicago","doi-asserted-by":"publisher","award":["RSG 1207"],"award-info":[{"award-number":["RSG 1207"]}],"id":[{"id":"10.13039\/100007656","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007656","name":"Loyola University Chicago","doi-asserted-by":"publisher","award":["IPRS1171"],"award-info":[{"award-number":["IPRS1171"]}],"id":[{"id":"10.13039\/100007656","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Phosducin-like proteins (PhLP) are thioredoxin domain-containing proteins that are highly conserved across unicellular and multicellular organisms. PhLP family proteins are hypothesized to function as co-chaperones in the folding of cytoskeletal proteins. Here, we present the initial molecular, biochemical, and functional characterization of\n                    <jats:italic>CG4511<\/jats:italic>\n                    as\n                    <jats:italic>Drosophila melanogaster PhLP3<\/jats:italic>\n                    . We cloned the gene into a bacterial expression vector and produced enzymatically active recombinant PhLP3, which showed similar kinetics to previously characterized orthologues. A fly strain homozygous for a P-element insertion in the 5\u2019 UTR of the\n                    <jats:italic>PhLP3<\/jats:italic>\n                    gene exhibited significant downregulation of\n                    <jats:italic>PhLP3<\/jats:italic>\n                    expression. We found these male flies to be sterile. Microscopic analysis revealed altered testes morphology and impairment of spermiogenesis, leading to a lack of mature sperm. Among the most significant observations was the lack of actin cones during sperm maturation. Excision of the P-element insertion in\n                    <jats:italic>PhLP3<\/jats:italic>\n                    restored male fertility, spermiogenesis, and seminal vesicle size. Given the high level of conservation of PhLP3, our data suggests PhLP3 may be an important regulator of sperm development across species.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0306676","type":"journal-article","created":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T13:42:53Z","timestamp":1730382173000},"page":"e0306676","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["The evolutionarily conserved PhLP3 is essential for sperm development in Drosophila melanogaster"],"prefix":"10.1371","volume":"19","author":[{"given":"Christopher","family":"Petit","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Kojak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3701-7064","authenticated-orcid":true,"given":"Samantha","family":"Webster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Marra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9196-1240","authenticated-orcid":true,"given":"Brendan","family":"Sweeney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9478-4728","authenticated-orcid":true,"given":"Claire","family":"Chaikin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer C.","family":"Jemc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0447-9197","authenticated-orcid":true,"given":"Stefan M.","family":"Kanzok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,10,31]]},"reference":[{"key":"pone.0306676.ref001","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/S0092-8674(00)81376-8","article-title":"Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin","volume":"87","author":"R Gaudet","year":"1996","journal-title":"Cell"},{"key":"pone.0306676.ref002","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1356-9597.2004.00708.x","article-title":"An evolutionarily conserved gene required for proper microtubule architecture in Caenorhabditis elegans","volume":"9","author":"S 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