{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T21:54:45Z","timestamp":1788126885749,"version":"build-2803163510"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"\nBackground<\/jats:title>\nWounds significantly affect people\u2019s quality of life and the clinical and financial burden of healthcare systems around the world. Many of the current drugs used to treat wounds have problems such as; allergies and drug resistance. Hence, the exploration of new therapeutic agents from natural origin may avert this problem. Clerodendrum myricoides<\/jats:italic> have long been used to treat wounds in Ethiopia. Despite this, nothing has so far been reported about the wound healing and anti-inflammatory activity of C<\/jats:italic>. myricoides<\/jats:italic>. This study aimed to evaluate the wound healing and anti-inflammatory activity of 80% methanol extract and solvent fractions of C<\/jats:italic>. myricoides<\/jats:italic> leaves in mice.<\/jats:p>\n<\/jats:sec>\n\nMethods<\/jats:title>\nLeaves of C<\/jats:italic>. myricoides<\/jats:italic> were extracted using the maceration technique. The extract was formulated as 5% and 10% w\/w ointments. The wound healing activity of the extract was evaluated using excision, incision, and burn wound models whereas the healing activities of solvent fractions were evaluated using the excision wound model. A carrageenan-induced paw edema model was used for the anti-inflammatory test.<\/jats:p>\n<\/jats:sec>\n\nResults<\/jats:title>\nIn the dermal toxicity test, 2000 mg\/kg of 10% extract was found to be safe. In excision and burn wound models, treatment with 10% and 5% extract showed a significant (p<0.001) wound contraction. Solvent fractions of the extract significantly reduced wound contraction. A significant reduction in periods of epithelialization and favorable histopathology changes were shown by extract ointments. In incision wounds, 10% (p<0.001) and 5% (p<0.01) extracts significantly increase skin-breaking strength. After one hour of treatment, 400 mg\/kg (p<0.001) and 200 mg\/kg (p<0.05) showed significant reduction in paw edema.<\/jats:p>\n<\/jats:sec>\n\nConclusion<\/jats:title>\nResults of this study indicate that 80% methanol extract and the solvent fraction of the leaves of C<\/jats:italic>. myricoides<\/jats:italic> possess wound-healing and anti-inflammatory activity and support traditional claims.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0306766","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T17:33:31Z","timestamp":1720632811000},"page":"e0306766","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of wound healing and anti-inflammatory activity of hydro-alcoholic extract and solvent fractions of the leaves of Clerodendrum myricoides (Lamiaceae) in mice"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9867-5443","authenticated-orcid":true,"given":"Alemante Tafese","family":"Beyna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assefa Kebad","family":"Mengesha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0111-407X","authenticated-orcid":true,"given":"Ermias Teklehaimanot","family":"Yefter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wubayehu","family":"Kahaliw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,7,10]]},"reference":[{"key":"pone.0306766.ref001","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/8852759","article-title":"The role of oxidative stress and antioxidants in diabetic wound healing","volume":"2021","author":"L. 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