{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:10:10Z","timestamp":1791940210221,"version":"4.3.4"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T00:00:00Z","timestamp":1720742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Nile Tilapia (<jats:italic>Oreochromis niloticus<\/jats:italic>) management procedures are directly linked to the final quality of the product. The aim of this study was to evaluate the effect of pre-slaughter density and different stunning methods on biochemical, respiratory and muscle injury parameters associated with quality and sensory characteristics of Nile tilapia fillets. Fish with an average weight of 762\u00b1105 g were used, first collected called the control group. The experiment was conducted in a 2 \u00d7 2 factorial scheme, with two densities (50 and 300 kg of live weight m<jats:sup>\u22123<\/jats:sup>) and two stunning methods thus totaling four treatments, with 15 repetitions per treatment totaling 75 fish sampled. Blood gas analysis, evaluation of biochemical parameters, analysis of meat quality and sensory analysis were carried out. For blood gas, biochemical and enzymatic parameters, the highest values were obtained for the density of 300 kg m<jats:sup>\u22123<\/jats:sup> and asphyxia method: partial pressures of CO2; glucose and lactate, the highest values presented were 268.98 and 11.33 mg dL<jats:sup>\u22121<\/jats:sup> respectively. As well as enzymatic activities, Creatinine kinase (CPK); Creatinine kinase isoenzyme (CKMB) showed higher values (768.93 and 1078.98 mg dL<jats:sup>\u22121<\/jats:sup> respectively) in the higher density and asphyxia method. Conversely, when evaluating the quality parameters, the highest values were observed for lower density and thermonarcosis. High depuration density (300 kg m<jats:sup>\u22123<\/jats:sup>), combined with the asphyxiation stunning method, promotes changes in respiratory dynamics and provides greater stress, less firm fillet texture and greater weight loss due to cooking, as well as changes in creatine kinase (CK) and its CK-MB isoenzyme, demonstrating greater muscle damage. On the other hand, the density of 50 kg m<jats:sup>\u22123<\/jats:sup> during pre-slaughter, combined with the method of stunning by thermonarcosis, provide a longer period of permanence in pre rigor mortis, which will result in fillets with a better sensory profile.<\/jats:p>","DOI":"10.1371\/journal.pone.0306880","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T17:26:06Z","timestamp":1720805166000},"page":"e0306880","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Respiratory and muscular effort during pre-slaughter stress affect Nile tilapia fillet quality"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1026-8273","authenticated-orcid":true,"given":"Silvia","family":"Prestes dos Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ildilene","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Cesar","family":"Godoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Geovanni","family":"De Almeida Banhara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio Douglas","family":"Goes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elenice","family":"Souza dos Reis Goes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claucia A.","family":"Honorato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,7,12]]},"reference":[{"issue":"January","key":"pone.0306880.ref001","doi-asserted-by":"crossref","first-page":"100611","DOI":"10.1016\/j.aqrep.2021.100611","article-title":"Aquaculture in Brazil: past, present and future","volume":"19","author":"WC Valenti","year":"2021","journal-title":"Aquac Reports"},{"key":"pone.0306880.ref002","first-page":"196","volume":"8","author":"V Lewandowski","year":"2015","journal-title":"Revista Agrarian. Agrarian"},{"issue":"1","key":"pone.0306880.ref003","doi-asserted-by":"crossref","first-page":"101","DOI":"10.4025\/actascianimsci.v32i1.6909","article-title":"Elabora\u00e7\u00e3o de croquete de til\u00e1pia do Nilo (Oreochromis niloticus) a partir de CMS e aparas do corte em \u201cV\u201d do fil\u00e9 e sua avalia\u00e7\u00e3o f\u00edsico-qu\u00edmica, microbiol\u00f3gica e sensorial","volume":"32","author":"AC Bordignon","year":"2010","journal-title":"Acta Sci \u2010 Anim Sci"},{"key":"pone.0306880.ref004","article-title":"Protozoan and helminths infestation of Nile tilapia Oreochromis niloticus and its correlation with certain water quality variables along river Nile in the area of Greater Cairo","volume":"345","author":"HMM El-Khayat","year":"2024","journal-title":"Environ Pollut"},{"issue":"S1","key":"pone.0306880.ref005","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/raq.12738","article-title":"From Africa to the world-The journey of Nile tilapia","volume":"15","author":"AFM El-Sayed","year":"2023","journal-title":"Rev Aquac"},{"key":"pone.0306880.ref006","unstructured":"FAO. World Fisheries and Aquaculture, FAO:Rome,2024 [Internet]. 2024. 1\u201311 p. Available from: https:\/\/www.fao.org\/3\/ca9229en\/online\/ca9229en.html#chapter-1_1."},{"issue":"1","key":"pone.0306880.ref007","first-page":"1","article-title":"Imbalance of the redox system and quality of tilapia fillets subjected to pre-slaughter stress","volume":"14","author":"ESR Goes","year":"2019","journal-title":"PLoS One"},{"issue":"3","key":"pone.0306880.ref008","first-page":"270","article-title":"Effect of different stocking densities on pre-slaughter stress based on respiratory parameters in Nile tilapia (Oreochromis niloticus)","volume":"16","author":"DGDA Banhara","year":"2021","journal-title":"Panam J Aquat Sci"},{"issue":"4","key":"pone.0306880.ref009","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1080\/1828051X.2018.1426396","article-title":"Effects of different stunning methods on blood markers and enzymatic activity of stress responses of tilapia (Oreochromis niloticus)","volume":"17","author":"FP Venturini","year":"2018","journal-title":"Ital J Anim Sci"},{"issue":"1","key":"pone.0306880.ref010","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1590\/fst.00518","article-title":"Goes ESDR, de Almeida FLA, de Oliveira CAL, Ribeiro RP. Anoxia stress and effect on flesh quality and gene expression of tilapia","volume":"39","author":"JSG Zuanazzi","year":"2019","journal-title":"Food Sci Technol"},{"issue":"1\u20134","key":"pone.0306880.ref011","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.aquaculture.2006.07.049","article-title":"Pre-slaughter crowding stress and killing procedures affecting quality and welfare in sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata)","volume":"263","author":"M Bagni","year":"2007","journal-title":"Aquaculture"},{"issue":"4","key":"pone.0306880.ref012","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1590\/1678-992x-2016-0387","article-title":"Effects of transportation stress on quality and sensory profiles of nile tilapia fillets","volume":"75","author":"R Goes ES dos","year":"2018","journal-title":"Sci Agric"},{"issue":"5","key":"pone.0306880.ref013","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0233636","article-title":"Resting time before slaughter restores homeostasis, increases rigor mortis time and fillet quality of surubim Pseudoplatystoma spp","volume":"15","author":"LE Fantini","year":"2020","journal-title":"PLoS One"},{"issue":"10","key":"pone.0306880.ref014","doi-asserted-by":"crossref","first-page":"5034","DOI":"10.1111\/are.15375","article-title":"Physiological stress and meat quality of pacu (Piaractus mesopotamicus) submitted to CO2 narcosis, hypothermia and electrical stunning","volume":"52","author":"PRC Oliveira Filho","year":"2021","journal-title":"Aquac Res"},{"issue":"2","key":"pone.0306880.ref015","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1590\/1981-6723.1115","article-title":"Influ\u00eancia do estresse causado pelo transporte e m\u00e9todo de abate sobre o rigor mortis do tambaqui (colossoma macropomum) influence of transport stress and slaughter method on rigor mortis of tambaqui (colossoma macropomum)","volume":"18","author":"JM Mendes","year":"2015","journal-title":"Brazilian J Food Technol"},{"issue":"July","key":"pone.0306880.ref016","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.aquaculture.2018.07.035","article-title":"Electrical stunning in pacu (Piaractus mesopotamicus) using direct current waveform","volume":"497","author":"DS Rucinque","year":"2018","journal-title":"Aquaculture"},{"key":"pone.0306880.ref017","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.aquaculture.2009.11.007","article-title":"Effect of stocking density and feeding level on energy expenditure and stress responsiveness in European sea bass Dicentrarchus labrax","volume":"298","author":"I Lupatsch","year":"2010","journal-title":"Aquaculture"},{"key":"pone.0306880.ref018","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.aquaculture.2014.05.040","article-title":"Effect of carbon monoxide for Atlantic salmon (Salmo salar L.) slaughtering on stress response and fillet shelf life","volume":"433","author":"A Concollato","year":"2014","journal-title":"Aquaculture"},{"key":"pone.0306880.ref019","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.foodchem.2014.11.111","article-title":"Pre-mortem stress and the subsequent effect on flesh quality of pre-rigor filleted Atlantic salmon (Salmo salar L.) during ice storage","volume":"175","author":"J Lerfall","year":"2015","journal-title":"Food Chem"},{"issue":"3\u20134","key":"pone.0306880.ref020","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0044-8486(99)00273-2","article-title":"Muscle activity at slaughter: I. Changes in flesh colour and gaping in rainbow trout","volume":"182","author":"DHF Robb","year":"2000","journal-title":"Aquaculture"},{"issue":"1","key":"pone.0306880.ref021","first-page":"141","article-title":"Effects of clove oil on Behavior and Flesh quality of common carp (Cyprinus carpio L.) in comparison with Pre-slaughter CO2 stunning, chilling and asphyxia","volume":"11","author":"K Rahmanifarah","year":"2011","journal-title":"Turkish J Fish Aquat Sci"},{"issue":"1","key":"pone.0306880.ref022","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.aquaculture.2005.05.016","article-title":"Relevance of dietary oil source for contraction and quality of pre-rigor filleted Atlantic cod, Gadus morhua","volume":"251","author":"T. M\u00f8rk\u00f8re","year":"2006","journal-title":"Aquaculture"},{"key":"pone.0306880.ref023","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1017\/S0962728600024854","article-title":"Methods used to kill fish: Field observations and literature reviewed","volume":"11","author":"KS Robb DHF","year":"2002","journal-title":"Anim Welf"},{"issue":"6","key":"pone.0306880.ref024","first-page":"1","article-title":"Pre-slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the Nile tilapia","volume":"10","author":"ESR Goes","year":"2015","journal-title":"PLoS One"},{"issue":"2\u20134","key":"pone.0306880.ref025","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.aquaculture.2006.10.035","article-title":"Effects of rested-harvest using the anesthetic AQUI-STM on channel catfish, Ictalurus punctatus, physiology and fillet quality","volume":"262","author":"BG Bosworth","year":"2007","journal-title":"Aquaculture"},{"issue":"2","key":"pone.0306880.ref026","first-page":"204","article-title":"O modelo dos \u201cCinco Dom\u00ednios\u201d do bem-estar animal aplicado em sistemas intensivos de produ\u00e7\u00e3o de bovinos, su\u00ednos e aves","volume":"19","author":"S Braga J da","year":"2018","journal-title":"Rev Bras Zooci\u00eancias"},{"issue":"5","key":"pone.0306880.ref027","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1111\/anu.12466","article-title":"A comprehensive evaluation of replacing fishmeal with housefly (Musca domestica) maggot meal in the diet of Nile tilapia (Oreochromis niloticus): growth performance, flesh quality, innate immunity and water environment","volume":"23","author":"L Wang","year":"2017","journal-title":"Aquac Nutr"},{"issue":"4","key":"pone.0306880.ref028","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1007\/s11259-023-10136-8","article-title":"Effect of different slaughter\/stunning methods on stress response, quality indicators and susceptibility to oxidation of large yellow croaker (Larimichthys crocea)","volume":"47","author":"Y Dong","year":"2023","journal-title":"Vet Res Commun"},{"issue":"10","key":"pone.0306880.ref029","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.3390\/foods13101477","article-title":"Effects of Stocking Density and Pre-Slaughter Handling on the Fillet Quality of Largemouth Bass (Micropterus salmoides): Implications for Fish Welfare","volume":"13","author":"N Hematyar","year":"2024","journal-title":"Foods"},{"key":"pone.0306880.ref030","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.740541","article-title":"Stunning\/slaughtering by cold shock in saline water: Effects on fish stress, post-mortem changes, and product quality in rainbow trout","volume":"582","author":"F Bordignon","year":"2024","journal-title":"Aquaculture"},{"issue":"july","key":"pone.0306880.ref031","first-page":"10791080","article-title":"Effect of different stunning methods on the shape of salmo carpio (l. 1758) fillets during rigor mortis and ice storage","volume":"12","author":"D Pulcini","year":"2022","journal-title":"Aquac eur"},{"issue":"11","key":"pone.0306880.ref032","first-page":"1877","volume":"29","author":"ER Ashwood","year":"1983","journal-title":"Temperature Correctionof Blood-Gasand pH Measurements"},{"issue":"3","key":"pone.0306880.ref033","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s00360-008-0306-4","article-title":"Differential sensitivity to capture stress assessed by blood acid-base status in five carcharhinid sharks","volume":"179","author":"JW Mandelman","year":"2009","journal-title":"J Comp Physiol B Biochem Syst Environ Physiol"},{"issue":"3\u20134","key":"pone.0306880.ref034","first-page":"307","article-title":"Clinical evaluation of a new creatine kinase MB activity reagent abrogating the effect of mitochondrial creatine kinase","volume":"59","author":"T. Hoshino","year":"2013","journal-title":"Clin Lab"},{"key":"pone.0306880.ref035","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"pone.0306880.ref036","unstructured":"Beutler, E. Reduced Glutathione (GSH). In: Bergmeyen, H.V. E. Red cell metabolism. A manual of biochemical methods. Grune Strat. 1975;2nd Editio:Nova York."},{"key":"pone.0306880.ref037","doi-asserted-by":"crossref","unstructured":"Mccords JM, Fridovich I. Superoxide Dismutase an enzymic function for erythrocuprein (hemocuprein)*. Vol. 244, The journal of biolooicai, ciiemistry. 1969.","DOI":"10.1016\/S0021-9258(18)63504-5"},{"key":"pone.0306880.ref038","first-page":"89","article-title":"Studies on rigor-mortis of fish-1. Difference in the mode of rigor-mortis among some varieties of fish by modified cutting\u2019s method","volume":"109","author":"M. Bito","year":"1983","journal-title":"Bull Tokai Reg Rab"},{"issue":"3","key":"pone.0306880.ref039","doi-asserted-by":"crossref","first-page":"455","DOI":"10.4141\/cjas96-066","article-title":"Estimates and detection of the PSE problem in young turkey breast meat","volume":"76","author":"S Barbut","year":"1996","journal-title":"Can J Anim Sci"},{"issue":"5","key":"pone.0306880.ref040","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1093\/ps\/76.5.785","article-title":"Effect of Muscle Opposition during Rigor on Development of Broiler Breast Meat Tenderness","volume":"76","author":"JA Cason","year":"1997","journal-title":"Poult Sci"},{"issue":"2","key":"pone.0306880.ref041","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1745-459X.1989.tb00463.x","article-title":"Designs To Balance the Effect of Order of Presentation and First\u2010Order Carry\u2010Over Effects in Hall Tests","volume":"4","author":"HJ Macfie","year":"1989","journal-title":"J Sens Stud"},{"key":"pone.0306880.ref042","unstructured":"R Core Team. R: R Project for Statistical Computing. R Found Stat Comput [Internet]. 2019;2. Available from: https:\/\/www.r-project.org\/."},{"key":"pone.0306880.ref043","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1093\/biomet\/52.3-4.591","article-title":"An analysis of variance test for normality (complete samples)","volume":"52","author":"WM Shapiro SS","year":"1965","journal-title":"Biometrika"},{"key":"pone.0306880.ref044","first-page":"278","article-title":"Robust Tests for Equality of Variances.\u201d In Contributions to Probability and Statistics, Edited","author":"H Levene","year":"1960","journal-title":"Ed by Olkin"},{"key":"pone.0306880.ref045","first-page":"3","volume":"1","author":"F. Mediburu","year":"2023","journal-title":"agricolae: Statistical Procedures for Agricultural Research"},{"issue":"April","key":"pone.0306880.ref046","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.aquaculture.2019.04.040","article-title":"Impact of fish density on water quality and physiological response of golden pompano (Trachinotus ovatus) flingerlings during transportation","volume":"507","author":"J Hong","year":"2019","journal-title":"Aquaculture"},{"issue":"2","key":"pone.0306880.ref047","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0228454","article-title":"Physiological and flesh quality consequences of pre-mortem crowding stress in Atlantic mackerel (Scomber scombrus)","volume":"15","author":"N Anders","year":"2020","journal-title":"PLoS One"},{"issue":"1\u20134","key":"pone.0306880.ref048","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.aquaculture.2004.03.018","article-title":"Physiological responses in Eurasian perch (Perca fluviatilis, L.) subjected to stress by transport and handling","volume":"237","author":"L Acerete","year":"2004","journal-title":"Aquaculture"},{"issue":"4","key":"pone.0306880.ref049","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1111\/faf.12158","article-title":"An overview of stress physiology of fish transport: changes in water quality as a function of transport duration","volume":"17","author":"FDF Sampaio","year":"2016","journal-title":"Fish Fish"},{"issue":"3\u20134","key":"pone.0306880.ref050","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.aquaculture.2008.09.014","article-title":"Dietary supplementation of l-tryptophan mitigates crowding stress and augments the growth in Cirrhinus mrigala fingerlings","volume":"293","author":"CS Tejpal","year":"2009","journal-title":"Aquaculture"},{"issue":"3","key":"pone.0306880.ref051","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/s10695-008-9259-5","article-title":"Nutritional regulation of hepatic glucose metabolism in fish","volume":"35","author":"P Enes","year":"2009","journal-title":"Fish Physiol Biochem"},{"issue":"January","key":"pone.0306880.ref052","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.cbpa.2019.01.019","article-title":"Modulation of stress and innate immune response by corticosteroids in pacu (Piaractus mesopotamicus)","volume":"231","author":"FS Zanuzzo","year":"2019","journal-title":"Comp Biochem Physiol -Part A Mol Integr Physiol"},{"key":"pone.0306880.ref053","first-page":"24","article-title":"Senci\u00eancia e bem-estar de peixes: uma vis\u00e3o de futuro do mercado consumidor","volume":"102","author":"AAS Pedrazzani","year":"2007","journal-title":"Panor da Aq\u00fcicultura"},{"issue":"1","key":"pone.0306880.ref054","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s10695-011-9561-5","article-title":"Safeguarding the welfare of farmed fish at harvest","volume":"38","author":"JA Lines","year":"2012","journal-title":"Fish Physiol Biochem"},{"issue":"3","key":"pone.0306880.ref055","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1046\/j.1365-2109.2003.00804.x","article-title":"Is humane slaughter of fish possible for industry?","volume":"34","author":"H Van De Vis","year":"2003","journal-title":"Aquac Res"},{"key":"pone.0306880.ref056","doi-asserted-by":"crossref","DOI":"10.1017\/S096272860000004X","article-title":"Live chilling of turbot and subsequent effect on behaviour, muscle stiffness, muscle quality, blood gases and chemistry","volume":"18","author":"B Roth","year":"2009","journal-title":"Anim Welf"},{"issue":"5","key":"pone.0306880.ref057","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1007\/s10695-016-0229-z","article-title":"Serum biochemical and non-specific immune responses of rainbow trout (Oncorhynchus mykiss) to dietary nucleotide and chronic stress","volume":"42","author":"M Yousefi","year":"2016","journal-title":"Fish Physiol Biochem"},{"key":"pone.0306880.ref058","doi-asserted-by":"crossref","unstructured":"Inoue LAKA, Hackbarth A, Arberl\u00e1ez-Rojas G, Moraes G. Growth performance and metabolism of the Neotropical fish Piaractus mesopotamicus under sustained swimming. Aquaculture [Internet]. 2019;511(April):734219. Available from: https:\/\/doi.org\/10.1016\/j.aquaculture.2019.734219.","DOI":"10.1016\/j.aquaculture.2019.734219"},{"issue":"April","key":"pone.0306880.ref059","doi-asserted-by":"crossref","first-page":"100908","DOI":"10.1016\/j.cbd.2021.100908","article-title":"Physiological responses and molecular strategies in heart of silver carp (Hypophthalmichthys molitrix) under hypoxia and reoxygenation","volume":"40","author":"X Li","year":"2021","journal-title":"Comp Biochem Physiol \u2010 Part D Genomics Proteomics"},{"issue":"5","key":"pone.0306880.ref060","article-title":"The Effect of Hot Water Extract of Tilapia on Exercise Capacity in Mice","volume":"12","author":"HY Tsai","year":"2022","journal-title":"Appl Sci"},{"key":"pone.0306880.ref061","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.meatsci.2016.05.009","article-title":"Blood parameters and electroencephalographic responses of goats to slaughter without stunning","volume":"121","author":"AB Sabow","year":"2016","journal-title":"Meat Sci"},{"key":"pone.0306880.ref062","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.aquaculture.2008.10.012","article-title":"Comparison of two stunning\/slaughtering methods on stress response and quality indicators of European sea bass (Dicentrarchus labrax)","volume":"287","author":"L Acerete","year":"2009","journal-title":"Aquaculture"},{"issue":"1\u20132","key":"pone.0306880.ref063","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s10499-004-9035-1","article-title":"Fish welfare and quality as affected by pre-slaughter and slaughter management","volume":"13","author":"BM Poli","year":"2005","journal-title":"Aquac Int"},{"issue":"1\u20134","key":"pone.0306880.ref064","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0044-8486(01)00874-2","article-title":"Freezing of pre-rigor fillets of Atlantic salmon","volume":"212","author":"O Einen","year":"2002","journal-title":"Aquaculture"},{"issue":"1","key":"pone.0306880.ref065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2109.2003.00754.x","article-title":"Effect of the slaughter method on the quality of raw and smoked eels (Anguilla anguilla L.)","volume":"34","author":"M Morzel","year":"2003","journal-title":"Aquac Res"},{"issue":"1\u20134","key":"pone.0306880.ref066","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.aquaculture.2010.04.020","article-title":"Effect of harvesting stress and slaughter conditions on selected flesh quality criteria of gilthead seabream (Sparus aurata)","volume":"305","author":"E Matos","year":"2010","journal-title":"Aquaculture"},{"key":"pone.0306880.ref067","doi-asserted-by":"crossref","unstructured":"Feiner G. Colour in fresh meat and in cured meat products. In: Meat Products Handbook: Practical Science and Technology. CambridgeWoodhead Publ Ltd. 2006;142\u201356.","DOI":"10.1533\/9781845691721.1.142"},{"issue":"2","key":"pone.0306880.ref068","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1590\/S1519-99402014000200023","article-title":"Fil\u00e9s de tambacu submetidos a salga seca e salga \u00famida","volume":"15","author":"V. A. Nates","year":"2014","journal-title":"Rev Bras Saude e Prod Anim"},{"issue":"7","key":"pone.0306880.ref069","doi-asserted-by":"crossref","first-page":"1","DOI":"10.36560\/14720211326","article-title":"Caracter\u00edsticas da carne de novilhos Nelore e F1 Rubia Gallega x Nelore suplementados com cromo","volume":"14","author":"FF Faria","year":"2021","journal-title":"Sci Electron Arch"},{"issue":"3","key":"pone.0306880.ref070","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1016\/j.foodchem.2012.03.038","article-title":"Effects of pre-slaughter stress on proteolytic enzyme activities and muscle quality of farmed Atlantic cod (Gadus morhua)","volume":"134","author":"L Hultmann","year":"2012","journal-title":"Food Chem"},{"key":"pone.0306880.ref071","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.aquaculture.2010.10.008","article-title":"Fillet texture and protease activities in different families of farmed atlantic Salmon (Salmo salar L.)","volume":"310","author":"D Bahuaud","year":"2010","journal-title":"Aquaculture"},{"issue":"8","key":"pone.0306880.ref072","first-page":"1190","article-title":"Electrical stunning of farmed Atlantic cod Gadus morhua L.: A comparison of an industrial and experimental method","volume":"41","author":"H Digre","year":"2010","journal-title":"Aquac Res"},{"issue":"3","key":"pone.0306880.ref073","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.foodchem.2009.05.028","article-title":"Muscle structure responses and lysosomal cathepsins B and L in farmed Atlantic salmon (Salmo salar L.) pre- and post-rigor fillets exposed to short and long-term crowding stress","volume":"118","author":"D Bahuaud","year":"2010","journal-title":"Food Chem"},{"issue":"4","key":"pone.0306880.ref074","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.meatsci.2009.11.007","article-title":"Oxidative status, in vitro iron-induced lipid oxidation and superoxide dismutase, catalase and glutathione peroxidase activities in rhea meat","volume":"84","author":"A Terevinto","year":"2010","journal-title":"Meat Sci"},{"issue":"1\u20134","key":"pone.0306880.ref075","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.aquaculture.2004.02.030","article-title":"Texture, gaping and colour of fresh and frozen Atlantic salmon flesh as affected by pre-slaughter iso-eugenol or CO2 anaesthesia","volume":"236","author":"A Kiessling","year":"2004","journal-title":"Aquaculture"},{"issue":"1","key":"pone.0306880.ref076","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/mnfr.201000453","article-title":"Protein oxidation in muscle foods: A review","volume":"55","author":"MN Lund","year":"2011","journal-title":"Mol Nutr Food Res"},{"key":"pone.0306880.ref077","first-page":"150","article-title":"Evaluation of water retention capacity in chicken breasts duet final pH","volume":"17","author":"VF Roque-Specht","year":"2009","journal-title":"Curr Agric Sci Technol"},{"key":"pone.0306880.ref078","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2016.08.004","article-title":"The impact of stunning methods on stress conditions and quality of silver carp (Hypophthalmichthys molitrix) fillets stored at 4\u00b0C during 72h postmortem","volume":"216","author":"L Zhang","year":"2017","journal-title":"Food Chemistry"},{"issue":"2","key":"pone.0306880.ref079","first-page":"120","article-title":"Sensory characteristics of different cod products related to consumer preferences and attitudes. Food Qual Prefer","volume":"20","author":"K Sveinsd\u00f3ttir","year":"2009","journal-title":"Internet]"},{"issue":"1","key":"pone.0306880.ref080","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.meatsci.2006.04.014","article-title":"Reducing salt: A challenge for the meat industry","volume":"74","author":"E. Desmond","year":"2006","journal-title":"Meat Sci"},{"key":"pone.0306880.ref081","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.foodchem.2014.01.118","article-title":"Rapid and non-destructive determination of drip loss and pH distribution in farmed Atlantic salmon (Salmo salar) fillets using visible and near-infrared (Vis-NIR) hyperspectral imaging","volume":"156","author":"HJ He","year":"2014","journal-title":"Food Chem"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0306880","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T17:26:59Z","timestamp":1720805219000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0306880"}},"subtitle":[],"editor":[{"given":"Sanjay Kumar","family":"Gupta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,7,12]]},"references-count":81,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0306880","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,12]]}}}