{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:56:04Z","timestamp":1789563364395,"version":"build-2803163510"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-00802-20"],"award-info":[{"award-number":["APQ-00802-20"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Conselho Nacional de Desenvolvimento Cientifico e Tecnol\u00f3gico, Brasil","award":["408146\/2021-4"],"award-info":[{"award-number":["408146\/2021-4"]}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["1324553\/PAPG 2017-20"],"award-info":[{"award-number":["1324553\/PAPG 2017-20"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Cutaneous leishmaniasis (CL) is a global public health problem caused by species on the genus <jats:italic>Leishmania<\/jats:italic> and is the most prevalent clinical form of leishmaniasis. The aim of this study was to develop a new LAMP assay for <jats:italic>Leishmania<\/jats:italic> sp. based on HSP70 gene and evaluate it clinically for molecular diagnosis of CL. The study was carried out in the following stages: i) design of primers based on HSP70 gene of <jats:italic>Leishmania<\/jats:italic> sp.; ii) evaluation of detection limit and analytical specificity; iii) estimation of the accuracy of LAMP-Leish\/HSP70 assay for diagnosing CL. A total of 100 skin biopsy samples from patients, comprising 60 CL cases and 40 non-cases, were analyzed in this study. One LAMP assay using HSP70 gene as molecular target were standardized, and the observed detection limit was 100fg of <jats:italic>L<\/jats:italic>. <jats:italic>braziliensis<\/jats:italic> purified DNA. The LAMP-Leish\/HSP70 assay was specific for <jats:italic>Leishmania<\/jats:italic> spp. The LAMP-Leish\/HSP70 assay showed an accuracy of 92%, and positivity rates were not affected by lesion onset time or parasite load. This novel LAMP assay targeting the HSP70 gene of <jats:italic>Leishmania<\/jats:italic> sp. has the potential to be a useful tool to integrate into routine diagnosis for suspected cases of CL.<\/jats:p>","DOI":"10.1371\/journal.pone.0306967","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T18:22:18Z","timestamp":1724350938000},"page":"e0306967","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development and accuracy evaluation of a new loop-mediated isothermal amplification assay targeting the HSP70 gene for the diagnosis of cutaneous leishmaniasis"],"prefix":"10.1371","volume":"19","author":[{"given":"Arthur Ribeiro Cheloni","family":"Soares","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f4nica Cardoso Santos de","family":"Faria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7907-7913","authenticated-orcid":true,"given":"Daniel Moreira de","family":"Avelar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,8,22]]},"reference":[{"key":"pone.0306967.ref001","article-title":"Status of endemicity of cutaneous leishmaniasis [Internet].","author":"World Health Organization","year":"2022","journal-title":"apps.who.int"},{"key":"pone.0306967.ref002","article-title":"Informe epidemiol\u00f3gico das Am\u00e9ricas [Internet].","author":"Pan-American Health Organization","year":"2022"},{"key":"pone.0306967.ref003","unstructured":"Expert Committee On The Control Of The Leishmaniases. Meeting, World Health Organization. Control of the leishmaniases: report of a meeting of the WHO Expert Committee on the Control of Leishmaniases, Geneva, 22\u201326 March 2010. Vol. 949. Geneva: World Health Organization; 2010."},{"issue":"106903","key":"pone.0306967.ref004","first-page":"106903","article-title":"New immunodiagnostic methods for human tegumentary leishmaniasis in the last 10 years","volume":"242","author":"W Nunes","year":"2023","journal-title":"Technological prospecting. Acta tropica"},{"issue":"82","key":"pone.0306967.ref005","first-page":"21","article-title":"Neglected tropical diseases","volume":"26","author":"D. Molyneux","year":"2013","journal-title":"Community Eye Health [Internet]."},{"issue":"4","key":"pone.0306967.ref006","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1586\/eri.10.19","article-title":"Current diagnosis and treatment of cutaneous and mucocutaneous leishmaniasis.","volume":"8","author":"H Goto","year":"2010","journal-title":"Expert Review of Anti-infective Therapy."},{"key":"pone.0306967.ref007","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ebiom.2018.11.011","article-title":"The paradox of tegumentary leishmaniasis diagnosis: How the need for accurate and modern techniques is being addressed in low income areas.","volume":"38","author":"PS Kurizky","year":"2018","journal-title":"eBioMedicineDec"},{"issue":"4","key":"pone.0306967.ref008","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1590\/S0074-02761999000400014","article-title":"Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission.","volume":"94","author":"G Adolfo","year":"1999","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz.Jul 1"},{"issue":"9","key":"pone.0306967.ref009","doi-asserted-by":"crossref","first-page":"2246","DOI":"10.1128\/jcm.32.9.2246-2252.1994","article-title":"Diagnosis of cutaneous leishmaniasis and species discrimination of parasites by PCR and hybridization","volume":"32","author":"N Rodr\u00edguez","year":"1994","journal-title":"Journal of Clinical Microbiology"},{"issue":"4","key":"pone.0306967.ref010","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.diagmicrobio.2014.05.002","article-title":"Mucocutaneous leishmaniasis: accuracy and molecular validation of noninvasive procedures in a L. 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studies","volume":"58","author":"A Flahault","year":"2005","journal-title":"Journal of Clinical Epidemiology"},{"key":"pone.0306967.ref034","doi-asserted-by":"crossref","first-page":"109368","DOI":"10.1016\/j.vetpar.2021.109368","article-title":"Correlations between tissue parasite load and common clinical signs in dogs naturally infected by Leishmania infantum","volume":"291","author":"\u00daMR Chagas","year":"2021","journal-title":"Veterinary Parasitology"},{"issue":"4","key":"pone.0306967.ref035","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/S0035-9203(03)90066-6","article-title":"Detection of circulating Leishmania chagasi DNA for the non-invasive diagnosis of human infection","volume":"97","author":"J Disch","year":"2003","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"issue":"3","key":"pone.0306967.ref036","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1590\/S0074-02761994000300013","article-title":"Detection of Trypanosoma cruzi and Leishmania using the polymerase chain reaction.","volume":"89","author":"W Degrave","year":"1994","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz.Sep 1"},{"issue":"6","key":"pone.0306967.ref037","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1128\/JCM.00208-13","article-title":"Real-Time PCR Assay for Detection and Quantification of Leishmania (Viannia) Organisms in Skin and Mucosal Lesions: Exploratory Study of Parasite Load and Clinical Parameters.","volume":"51","author":"M Jara","year":"2013","journal-title":"Journal of Clinical Microbiology"},{"issue":"2","key":"pone.0306967.ref038","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.cmi.2018.04.025","article-title":"Accuracy of qPCR for quantifying Leishmania kDNA in different skin layers of patients with American tegumentary leishmaniasis","volume":"25","author":"L. Sevilha-Santos","year":"2019","journal-title":"Clinical microbiology and infection"},{"key":"pone.0306967.ref039","doi-asserted-by":"crossref","first-page":"790906","DOI":"10.3389\/fmicb.2021.790906","article-title":"Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis","volume":"12","author":"ML Freire","year":"2021","journal-title":"Frontiers in Microbiology"},{"issue":"1","key":"pone.0306967.ref040","doi-asserted-by":"crossref","first-page":"e0007981","DOI":"10.1371\/journal.pntd.0007981","article-title":"Test accuracy of polymerase chain reaction methods against conventional diagnostic techniques for Cutaneous Leishmaniasis (CL) in patients with clinical or epidemiological suspicion of CL: Systematic review and meta-analysis","volume":"14","author":"LE Mesa","year":"2020","journal-title":"PLOS Neglected Tropical Diseases"},{"issue":"1","key":"pone.0306967.ref041","first-page":"1","article-title":"Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects.","volume":"53","author":"T Notomi","year":"2015","journal-title":"Journal of Microbiology (Seoul, Korea)"},{"issue":"12","key":"pone.0306967.ref042","doi-asserted-by":"crossref","first-page":"63e63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"T Notomi","year":"2000","journal-title":"Nucleic Acids Research"},{"key":"pone.0306967.ref043","doi-asserted-by":"crossref","first-page":"105612","DOI":"10.1016\/j.micpath.2022.105612","article-title":"A novel rapid LAMP test for identification of cutaneous leishmaniasis: An evaluation and comparative analysis of three molecular methods","volume":"169","author":"S Salari","year":"2022","journal-title":"Microbial Pathogenesis"},{"issue":"11","key":"pone.0306967.ref044","doi-asserted-by":"crossref","first-page":"e21397","DOI":"10.1016\/j.heliyon.2023.e21397","article-title":"Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica.","volume":"9","author":"Y Taslimi","year":"2023","journal-title":"Heliyon"},{"key":"pone.0306967.ref045","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.exppara.2019.05.006","article-title":"evaluation of a loop-mediated isothermal amplification assay for rapid detection of Leishmania amazonensis in skin samples","volume":"203","author":"Roberta Lima Caldeira Celeste","year":"2019","journal-title":"Experimental parasitology"},{"issue":"3","key":"pone.0306967.ref046","doi-asserted-by":"crossref","first-page":"610","DOI":"10.3390\/microorganisms9030610","article-title":"Loop-Mediated Isothermal Amplification Allows Rapid, Simple and Accurate Molecular Diagnosis of Human Cutaneous and Visceral Leishmaniasis Caused by Leishmania infantum When Compared to PCR.","volume":"9","author":"AV Ibarra-Meneses","year":"2021","journal-title":"Microorganisms"},{"issue":"153","key":"pone.0306967.ref047","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.actatropica.2015.10.013","article-title":"A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool.","volume":"153","author":"CO Nzelu","year":"2016","journal-title":"Acta Tropica"},{"issue":"3","key":"pone.0306967.ref048","doi-asserted-by":"crossref","first-page":"167","DOI":"10.2144\/000113072","article-title":"Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue","volume":"46","author":"M Goto","year":"2009","journal-title":"BioTechniques"},{"issue":"1","key":"pone.0306967.ref049","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-017-2318-8","article-title":"Development of a rapid loop-mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection","volume":"17","author":"S Verma","year":"2017","journal-title":"BMC Infectious Diseases"},{"issue":"03","key":"pone.0306967.ref050","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1017\/S0031182006001570","article-title":"M. Requena J. Genomic organization and expression of the HSP70 locus in New and Old World Leishmania species","volume":"134","author":"C Folgueira","year":"2006","journal-title":"Parasitology"},{"issue":"3","key":"pone.0306967.ref051","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0001-706X(98)00100-4","article-title":"Leishmania (Viannia) braziliensis is the predominant species infecting patients with American cutaneous leishmaniasis in the State of Minas Gerais, Southeast Brazil.","volume":"72","author":"M Azeredo","year":"1999","journal-title":"Acta Tropica"},{"issue":"12","key":"pone.0306967.ref052","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1089\/vbz.2021.0038","article-title":"Diagnostic Efficacy of Recombinase-Polymerase-Amplification Coupled with Lateral Flow Strip Reading in Patients with Cutaneous Leishmaniasis from the Amazonas Rainforest of Per\u00fa.","volume":"21","author":"BL Travi","year":"2021","journal-title":"Vector borne and zoonotic diseases."},{"issue":"3","key":"pone.0306967.ref053","doi-asserted-by":"crossref","first-page":"489","DOI":"10.4269\/ajtmh.1987.36.489","article-title":"Diagnosis of Cutaneous and Mucocutaneous Leishmaniasis in Colombia: A Comparison of Seven Methods","volume":"36","author":"KA Weigle","year":"1987","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"issue":"10","key":"pone.0306967.ref054","doi-asserted-by":"crossref","first-page":"3768","DOI":"10.1128\/JCM.38.10.3768-3773.2000","article-title":"Diagnosis of Cutaneous Leishmaniasis in Colombia: the Sampling Site within Lesions Influences the Sensitivity of Parasitologic Diagnosis","volume":"38","author":"JR Ram\u00edrez","year":"2000","journal-title":"Journal of Clinical Microbiology"},{"issue":"7","key":"pone.0306967.ref055","first-page":"e0004865","article-title":"Prevalence and Hospital Management of Amphotericin B Deoxycholate-Related Toxicities during Treatment of HIV-Associated Cryptococcal Meningitis in South Africa. 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