{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:22:02Z","timestamp":1747196522424,"version":"3.40.5"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T00:00:00Z","timestamp":1722816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Roux-en-Y Gastric Bypass may be associated with an alteration of protein bioavailability in relation to intestinal remodeling. Our study aimed to test this hypothesis by Roux-en-Y Gastric Bypass. Diet-induced obese rats underwent Roux-en-Y Gastric Bypass surgery (RYGB rats) while a Sham-operated control group was used. All rats received a <jats:sup>15<\/jats:sup>N-labeled protein meal 1 or 3 months after surgery and were euthanized 6h later. Protein digestibility, <jats:sup>15<\/jats:sup>N recovered in organs and urea pool, fractional protein synthesis rate, and intestinal morphometry were assessed. Protein digestibility was similar in all groups (94.2\u00b10.3%). The small intestine was hypertrophied in RYGB rats 1 month after surgery, weighing 9.1\u00b10.2g vs. 7.0\u00b10.3g in Sham rats (P = 0.003). Villus height and crypt depth were increased in the alimentary limb and ileum of RYGB rats. However, Roux-en-Y Gastric Bypass had no impact on the fractional synthesis rate. In the gastrointestinal tract, <jats:sup>15<\/jats:sup>N retention only differed in the ileal mucosa and was higher in RYGB rats at 1 month (0.48\u00b10.2% vs. 0.3\u00b10.09%, P = 0.03). <jats:sup>15<\/jats:sup>N recovery from the liver, muscle, and skin was lower in RYGB rats at 1 month. <jats:sup>15<\/jats:sup>N recovery from urinary and plasma urea was higher in RYGB rats at both times, resulting in increased total deamination (13.2\u00b10.9% vs. 10.1\u00b10.5%, P&lt;0.01). This study showed that Roux-en-Y Gastric Bypass did not affect protein digestibility. Dietary nitrogen sequestration was transitorily and moderately diminished in several organs. This was associated with a sustained elevation of postprandial deamination after Roux-en-Y Gastric Bypass, whose mechanisms merit further studies.<\/jats:p>","DOI":"10.1371\/journal.pone.0307075","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T17:39:40Z","timestamp":1722879580000},"page":"e0307075","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of gastric bypass on the digestibility and postprandial metabolic fate of 15N dietary protein in 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