{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:00:37Z","timestamp":1767178837991,"version":"build-2238731810"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006071","name":"Facultad de Ciencias, Universidad de los Andes","doi-asserted-by":"publisher","award":["INV-2018-34-1286"],"award-info":[{"award-number":["INV-2018-34-1286"]}],"id":[{"id":"10.13039\/501100006071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006071","name":"Facultad de Ciencias, Universidad de los Andes","doi-asserted-by":"publisher","award":["INV-2019-85-1836"],"award-info":[{"award-number":["INV-2019-85-1836"]}],"id":[{"id":"10.13039\/501100006071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006071","name":"Facultad de Ciencias, Universidad de los Andes","doi-asserted-by":"publisher","award":["INV-2017-2"],"award-info":[{"award-number":["INV-2017-2"]}],"id":[{"id":"10.13039\/501100006071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["852 CD 1204-852-70312 (Young Researcher)"],"award-info":[{"award-number":["852 CD 1204-852-70312 (Young Researcher)"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006071","name":"Facultad de Ciencias, Universidad de los Andes","doi-asserted-by":"publisher","award":["INV-2021-128-2322"],"award-info":[{"award-number":["INV-2021-128-2322"]}],"id":[{"id":"10.13039\/501100006071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["852 CD 1204-852-70312"],"award-info":[{"award-number":["852 CD 1204-852-70312"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["852 CD 1204-852-70312"],"award-info":[{"award-number":["852 CD 1204-852-70312"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Worldwide incidence of kidney diseases has been rising. Thus, recent research has focused on zebrafish, whose fast development and innate regeneration capacity allow identifying factors influencing renal processes. Among these poorly studied factors are extracellular matrix (ECM) proteins like Fibronectin (Fn) essential in various tissues but not yet evaluated in a renal context. We utilized early\n                    <jats:italic>nat<\/jats:italic>\n                    and\n                    <jats:italic>han<\/jats:italic>\n                    zebrafish mutant embryos and carrier adults to investigate Fn\u2019s role during kidney development and regeneration. The locus natter (\n                    <jats:italic>nat<\/jats:italic>\n                    ) encodes Fn and the locus\n                    <jats:italic>han<\/jats:italic>\n                    encodes Hand2, which results in increased Fn deposition. Our results show that Fn impacts identity maintenance and morphogenesis during development and influences conditions for neonephrogenic cluster formation during regeneration. Histological analysis revealed disrupted pronephric structures and increased blood cell accumulation in Fn mutants. Despite normal expression of specification markers\n                    <jats:italic>(pax2<\/jats:italic>\n                    ,\n                    <jats:italic>ATP\u03b11a<\/jats:italic>\n                    .\n                    <jats:italic>1<\/jats:italic>\n                    ), structural abnormalities were evident. Differences between wild-type and mutation-carriers suggest a haploinsufficiency scenario. These findings reveal a novel function for ECM in renal development and regeneration, with potential implications for understanding and treating kidney diseases.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0307390","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T13:39:51Z","timestamp":1725629991000},"page":"e0307390","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fibronectin and Hand2 influence tubulogenesis during pronephros development and mesonephros regeneration in zebrafish (Danio rerio)"],"prefix":"10.1371","volume":"19","author":[{"given":"Lucia Carolina","family":"Uribe-Montes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilo Alfonso","family":"Sanabria-Camargo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian Camilo","family":"Pi\u00f1eros-Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n","family":"Ot\u00e1lora-Tarazona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2678-8011","authenticated-orcid":true,"given":"Estefan\u00eda","family":"\u00c1vila-Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin","family":"Acosta-Virg\u00fcez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5671-7017","authenticated-orcid":true,"given":"Zayra Viviana","family":"Garavito-Aguilar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,9,6]]},"reference":[{"issue":"5","key":"pone.0307390.ref001","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nrg2091","article-title":"Animals models of human disease: zebrafish swim into view","volume":"8","author":"G.J Lieschke","year":"2007","journal-title":"Nature reviews, genetics"},{"issue":"6","key":"pone.0307390.ref002","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/j.kint.2016.01.031","article-title":"Little fish, big catch: Zebrafish as a model for kidney disease","volume":"89","author":"S. 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Drummond","year":"2000","journal-title":"The zebrafish pronephros: A genetic system for studies of kidney development"},{"key":"pone.0307390.ref005","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4252\/wjsc.v8.i2.22","article-title":"Insights into kidney stem cell development and regeneration using zebrafish","volume":"8","author":"BE Drummond","year":"2016","journal-title":"World J Stem Cells"},{"issue":"7332","key":"pone.0307390.ref006","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/nature09669","article-title":"Identification of adult nephron progenitors capable of kidney regeneration in zebrafish","volume":"470","author":"C. Q. Diep","year":"2011","journal-title":"Nature"},{"issue":"7","key":"pone.0307390.ref007","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1242\/dmm.015545","article-title":"Zebrafish models in translational research: Tipping the scales toward advancements in human health","volume":"7","author":"J. B. Phillips","year":"2014","journal-title":"Disease Models & Mechanisms"},{"issue":"11","key":"pone.0307390.ref008","doi-asserted-by":"crossref","first-page":"367","DOI":"10.4252\/wjsc.v8.i11.367","article-title":"Renal progenitors: Roles in kidney disease and regeneration","volume":"8","author":"B. E. Chambers","year":"2016","journal-title":"World Journal of Stem Cells"},{"issue":"3","key":"pone.0307390.ref009","doi-asserted-by":"crossref","first-page":"483","DOI":"10.3390\/cells4030483","article-title":"Nephron patterning: Lessons from xenopus, zebrafish, and mouse studies","volume":"4","author":"A Desgrange","year":"2015","journal-title":"Cells"},{"key":"pone.0307390.ref010","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.biocel.2014.10.011","article-title":"Extracellular matrix considerations for scar-free repair and regeneration: Insights from regenerative diversity among vertebrates","volume":"56","author":"J. 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