{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T05:54:42Z","timestamp":1793166882855,"version":"4.5.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Rosenstadt Fund, University of Toronto"},{"name":"GoodHope Ehlers-Danlos Syndrome Foundation"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Ehlers-Danlos syndromes (EDS) represent a group of rare genetic disorders affecting connective tissues. Globally, approximately 1.5 million individuals suffer from EDS, with 10,000 reported cases in Canada alone. Understanding the histological properties of collagen in EDS has been challenging, but advanced techniques like atomic force microscopy (AFM) have opened up new possibilities for label-free skin imaging. This approach, which explores Type I collagen fibrils at the nanoscale, could potentially enhance EDS diagnosis and our knowledge of collagen type I-related connective tissue disorders. In the current study, we have employed AFM to examine ex-vivo skin biopsies from four individuals: one with classical EDS (cEDS), one with hypermobile EDS (hEDS), one with hEDS and Scleroderma (hEDS-Scleroderma), and one healthy control. Picrosirius red (PS) staining was used to highlight collagen differences in the samples. For each case, 14 images and 1400 force curves were obtained, with seven images and 700 force curves representing healthy collagen (PS-induced red staining) and the rest showcasing disrupted collagen (yellow staining). The results showed that PS staining was uniform throughout the control section, while cEDS and hEDS displayed localized areas of yellow staining. In the case of hEDS-Scleroderma, the yellow staining was widespread throughout the section. AFM images revealed irregular collagen fibrils in the disrupted, yellow-stained areas, contrasting with aligned and well-registered collagen fibrils in healthy, red-stained regions. Additionally, the study assessed the ability of non-AFM specialists to differentiate between healthy and disrupted collagen in AFM images, yielding substantial agreement among raters according to Fleiss\u2019s and Cohen\u2019s kappa scores (0.96 and 0.79\u00b10.1, respectively). Biomechanical analysis revealed that normal healthy collagen exhibited a predominant population at 2.5 GPa. In contrast, EDS-affected collagen displayed subpopulations with lower compressive elastic modulus, indicating weaker collagen fibrils in EDS patients. Although these findings pertain to a limited number of cases, they offer valuable insights into the nanoscale collagen structure and biomechanics in individuals with EDS. Over time, these insights could be developed into specific biomarkers for the condition, improving diagnosis and treatment for EDS and related connective tissue disorders.<\/jats:p>","DOI":"10.1371\/journal.pone.0307442","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T19:04:14Z","timestamp":1724353454000},"page":"e0307442","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Investigation of dermal collagen nanostructures in Ehlers-Danlos Syndrome (EDS) patients"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7951-559X","authenticated-orcid":true,"given":"Mehrnoosh","family":"Neshatian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nimish","family":"Mittal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiman","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Khattignavong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6322-7817","authenticated-orcid":true,"given":"Laurent","family":"Bozec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,8,22]]},"reference":[{"key":"pone.0307442.ref001","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s00403-018-1835-7","article-title":"Ex vivo nonlinear microscopy imaging of Ehlers\u2013Danlos syndrome-affected skin","volume":"310","author":"N Kiss","year":"2018","journal-title":"Arch Dermatol Res"},{"key":"pone.0307442.ref002","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1097\/01.JAA.0000657160.48246.91","article-title":"A review of Ehlers-Danlos syndrome","volume":"33","author":"E Miller","year":"2020","journal-title":"JAAPA"},{"key":"pone.0307442.ref003","doi-asserted-by":"crossref","DOI":"10.1097\/MD.0000000000013115","article-title":"Management of chronic pain in Ehlers\u2013Danlos syndrome: Two case reports and a review of literature","volume":"97","author":"Z Zhou","year":"2018","journal-title":"Medicine"},{"key":"pone.0307442.ref004","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1001\/jamafacial.2017.0793","article-title":"Characteristics, diagnosis, and management of Ehlers-Danlos syndromes: a review","volume":"20","author":"AW Joseph","year":"2018","journal-title":"JAMA Facial Plastic Surgery"},{"key":"pone.0307442.ref005","doi-asserted-by":"crossref","first-page":"e031365","DOI":"10.1136\/bmjopen-2019-031365","article-title":"Diagnosed prevalence of Ehlers-Danlos syndrome and hypermobility spectrum disorder in Wales, UK: a national electronic cohort study and case\u2013control comparison","volume":"9","author":"JC Demmler","year":"2019","journal-title":"BMJ open"},{"key":"pone.0307442.ref006","first-page":"165","article-title":"Bert Callewaert MD","volume":"22","author":"A. 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