{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T19:26:24Z","timestamp":1772220384705,"version":"3.50.1"},"reference-count":25,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T00:00:00Z","timestamp":1724716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NeoVentures Biotechnology Europe SAS"},{"DOI":"10.13039\/100002565","name":"Alzheimer\u2019s Drug Discovery Foundation","doi-asserted-by":"publisher","award":["\"Blood diagnostic test for AD: aptamer deep biomarker fingerprinting\" - GDAPB-201808-2016228"],"award-info":[{"award-number":["\"Blood diagnostic test for AD: aptamer deep biomarker fingerprinting\" - GDAPB-201808-2016228"]}],"id":[{"id":"10.13039\/100002565","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NeoVentures Biotechnology Inc."}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>An approach for the agnostic identification and validation of aptamers for the prediction of a medical state from plasma analysis is presented in application to a key risk factor for Alzheimer\u2019s disease. brain amyloid deposition. This method involved the use of a newly designed aptamer library with sixteen random nucleotides interspersed with fixed sequences called a Neomer library. The Neomer library approach enables the direct application of the same starting library on multiple plasma samples, without the requirement for pre-enrichment associated with the traditional approach. Eight aptamers were identified as a result of the selection process and screened across 390 plasma samples by qPCR assay. Results were analysed using multiple machine learning algorithms from the Scikit-learn package along with clinical variables including cognitive status, age and sex to create predictive models. An Extra Trees Classifier model provided the highest predictive power. The Neomer approach resulted in a sensitivity of 0.88. specificity of 0.76. and AUC of 0.79. The only clinical variables that were included in the model were age and sex. We conclude that the Neomer approach represents a clear improvement for the agnostic identification of aptamers (Aptamarkers) that bind to unknown biomarkers of a medical state.<\/jats:p>","DOI":"10.1371\/journal.pone.0307678","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T13:36:01Z","timestamp":1724765761000},"page":"e0307678","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["A reproducible approach for the use of aptamer libraries for the identification of Aptamarkers for brain amyloid deposition based on plasma analysis"],"prefix":"10.1371","volume":"19","author":[{"given":"Cathal","family":"Meehan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soizic","family":"Lecocq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7153-0426","authenticated-orcid":true,"given":"Gregory","family":"Penner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,8,27]]},"reference":[{"key":"pone.0307678.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2164-12-293","article-title":"RNA-seq: technical variability and sampling","volume":"12","author":"LM McIntyre","year":"2011","journal-title":"BMC Genomics"},{"issue":"7","key":"pone.0307678.ref002","doi-asserted-by":"crossref","first-page":"286","DOI":"10.3390\/metabo10070286","article-title":"Find the needle in the haystack, then find it again: replication and validation in the \u2018omics era","volume":"10","author":"W Perng","year":"2020","journal-title":"Metabolites"},{"issue":"1","key":"pone.0307678.ref003","doi-asserted-by":"crossref","first-page":"e0190212","DOI":"10.1371\/journal.pone.0190212","article-title":"Aptamers as biomarkers for neurological disorders. 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