{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:23:36Z","timestamp":1747196616549,"version":"3.40.5"},"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is a valuable therapeutic alternative for patients with peritoneal metastases. PIPAC uses a hyaluronic acid-based gel to reduce surgically induced adhesions. The aim of this study was to evaluate the effects of the hyaluronic acid-based gel on tumor dissemination. First, we explored whether the survival of CT26 luciferase-expressing murine colonic tumor cells was correlated with the dose of HyaRegen\u00ae Gel, and we determined the half-maximal inhibitory concentration (the IC50) of the gel. Next, we performed an in vitro study of cell survival rates after gel application on day 0 (D0) and day 1 (D1). Finally, we intraperitoneally administered the gel to mice with immunocompetent BALB\/c colonic peritoneal metastases (on D0, D5, D10, D14, and D18). Tumor growth was regularly monitored using a bioluminescence assay (on D11, D17, and D21). After all mice had been sacrificed on D21, the body weights and the volumes of intraperitoneal ascites were measured; the Peritoneal Carcinosis Index (PCI) and Ki-antigen 67 scores were calculated. The IC50 value was 70 \u03bcL of gel in a total volume of 100 \u03bcL. The cell survival rates on D4 were identical in the control group and the two groups that had been treated with gel on D0 and D1. The bioluminescence levels over time were similar in the gel and control groups. The PCI scores were 35.5 \u00b1 2.89 for the control group and 36 \u00b1 2.45 for the gel group (p = 0.8005). The mean Ki-67 index percentages were 37.28 \u00b11 1.75 for the control group and 34.03 \u00b1 8.62 for the gel group (p = 0.1971). This in vitro and in vivo study using a mouse model of immunocompetent metastatic peritoneal cancer did not reveal any pro- or anti-tumoral effect of HyaRegen\u00ae Gel. These findings indicate that the gel can be used to treat PIPACs with minimal apprehension.<\/jats:p>","DOI":"10.1371\/journal.pone.0307965","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T17:26:31Z","timestamp":1725989191000},"page":"e0307965","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of HyaRegen gel on tumour proliferation of colorectal peritoneal metastases"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4883-468X","authenticated-orcid":true,"given":"Marie-Laure","family":"Perrin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e9ment","family":"Bassetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvaine","family":"Durand Fontanier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Yardin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0217-6910","authenticated-orcid":true,"given":"Sylvia M.","family":"Bardet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1036-6474","authenticated-orcid":true,"given":"Abdelkader","family":"Taibi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"issue":"10207","key":"pone.0307965.ref001","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1016\/S0140-6736(19)32319-0","article-title":"Colorectal cancer.","volume":"394","author":"E Dekker","year":"2019","journal-title":"Lancet"},{"issue":"11","key":"pone.0307965.ref002","doi-asserted-by":"crossref","first-page":"3504","DOI":"10.1245\/s10434-013-3039-x","article-title":"Pressurized intraperitoneal aerosol chemotherapy (PIPAC): occupational health and safety aspects.","volume":"20","author":"W Solass","year":"2013","journal-title":"Ann Surg Oncol"},{"issue":"4","key":"pone.0307965.ref003","doi-asserted-by":"crossref","first-page":"931","DOI":"10.3390\/jcm11040931","article-title":"Chemically Modified Hyaluronic Acid for Prevention of Post-Surgical Adhesions: New Aspects of Gel Barriers Physical Profiles.","volume":"11","author":"LA Torres-de la Roche","year":"2022","journal-title":"J Clin Med. 11 f\u00e9vr"},{"key":"pone.0307965.ref004","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/978-1-4613-1247-5_23","article-title":"Clinical research methodologies in diagnosis and staging of patients with peritoneal carcinomatosis","volume":"82","author":"P Jacquet","year":"1996","journal-title":"Cancer Treat Res"},{"key":"pone.0307965.ref005","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/1471-2407-7-104","article-title":"Reduction of peritoneal carcinomatosis by intraperitoneal administration of phospholipids in rats","volume":"7","author":"J Otto","year":"2007","journal-title":"BMC Cancer"},{"issue":"6","key":"pone.0307965.ref006","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1007\/s12094-020-02525-1","article-title":"10 ns PEFs induce a histological response linked to cell death and cytotoxic T-lymphocytes in an immunocompetent mouse model of peritoneal metastasis","volume":"23","author":"A Taibi","year":"2021","journal-title":"Clin Transl Oncol. juin"},{"issue":"2","key":"pone.0307965.ref007","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1515\/pp-2016-0011","article-title":"Peritoneal sampling and histological assessment of therapeutic response in peritoneal metastasis: proposal of the Peritoneal Regression Grading Score (PRGS).","volume":"1","author":"W Solass","year":"2016","journal-title":"Pleura Peritoneum."},{"issue":"7","key":"pone.0307965.ref008","doi-asserted-by":"crossref","first-page":"e0220360","DOI":"10.1371\/journal.pone.0220360","article-title":"Comparison of implantation sites for the development of peritoneal metastasis in a colorectal cancer mouse model using non-invasive bioluminescence imaging","volume":"14","author":"A Taibi","year":"2019","journal-title":"PLoS One"},{"issue":"2","key":"pone.0307965.ref009","first-page":"166","article-title":"Ki67 assessment in breast cancer: an update","volume":"49","author":"F Penault-Llorca","year":"2017","journal-title":"Pathology. f\u00e9vr"},{"key":"pone.0307965.ref010","doi-asserted-by":"crossref","first-page":"606458","DOI":"10.1155\/2014\/606458","article-title":"Collagen VI and hyaluronan: the common role in breast cancer","volume":"2014","author":"E Karousou","year":"2014","journal-title":"Biomed Res Int"},{"key":"pone.0307965.ref011","first-page":"75","article-title":"Proteoglycans remodeling in cancer: Underlying molecular mechanisms","author":"AD Theocharis","year":"2019","journal-title":"Matrix Biol. janv"},{"issue":"3","key":"pone.0307965.ref012","doi-asserted-by":"crossref","first-page":"R84","DOI":"10.1186\/bcr3199","article-title":"CD44 enhances invasion of basal-like breast cancer cells by upregulating serine protease and collagen-degrading enzymatic expression and activity","volume":"14","author":"N Montgomery","year":"2012","journal-title":"Breast Cancer Res"},{"issue":"1","key":"pone.0307965.ref013","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1742-4658.2010.07919.x","article-title":"Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting","volume":"278","author":"C Gialeli","year":"2011","journal-title":"FEBS J. janv"},{"issue":"7\u20118","key":"pone.0307965.ref014","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1038\/sj.bjc.6690180","article-title":"Hyaluronan expression in gastric cancer cells is associated with local and nodal spread and reduced survival rate","volume":"79","author":"LP Set\u00e4l\u00e4","year":"1999","journal-title":"Br J Cancer"},{"issue":"1","key":"pone.0307965.ref015","first-page":"150","article-title":"High levels of stromal hyaluronan predict poor disease outcome in epithelial ovarian cancer","volume":"60","author":"MA Anttila","year":"2000","journal-title":"Cancer Res"},{"issue":"12","key":"pone.0307965.ref016","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1046\/j.1365-2168.2000.01586.x","article-title":"Hyaluronic acid as prognostic marker in resectable colorectal cancer","volume":"87","author":"A Llaneza","year":"2000","journal-title":"Br J Surg. d\u00e9c"},{"issue":"1","key":"pone.0307965.ref017","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s13048-018-0394-z","article-title":"Cross-linked hyaluronan gel inhibits the growth and metastasis of ovarian carcinoma.","volume":"11","author":"J Pang","year":"2018","journal-title":"J Ovarian Res. 6 mars"},{"issue":"40","key":"pone.0307965.ref018","doi-asserted-by":"crossref","first-page":"65418","DOI":"10.18632\/oncotarget.11739","article-title":"Cross-linked hyaluronic acid gel inhibits metastasis and growth of gastric and hepatic cancer cells: in vitro and in vivo studies.","volume":"7","author":"T Lan","year":"2016","journal-title":"Oncotarget"},{"issue":"7","key":"pone.0307965.ref019","doi-asserted-by":"crossref","first-page":"4589","DOI":"10.1074\/jbc.R100038200","article-title":"Signaling properties of hyaluronan receptors","volume":"277","author":"EA Turley","year":"2002","journal-title":"J Biol Chem"},{"issue":"4","key":"pone.0307965.ref020","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.yexcr.2010.11.017","article-title":"Hyaluronan in human malignancies","volume":"317","author":"RK Sironen","year":"2011","journal-title":"Exp Cell Res"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0307965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T17:27:23Z","timestamp":1725989243000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0307965"}},"subtitle":[],"editor":[{"given":"Fabrizio","family":"D\u2019Acapito","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,9,10]]},"references-count":20,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0307965","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2024,9,10]]}}}