{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T02:28:48Z","timestamp":1785292128686,"version":"3.55.0"},"reference-count":102,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["PE2147\/3-1"],"award-info":[{"award-number":["PE2147\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The archaeal isolate J.3.6.1-F.2.7.3<jats:sup>T<\/jats:sup> was obtained from an anaerobic enrichment culture, where it may play an important role in methane production during pyrite formation. The new isolate formed a species-level clade with <jats:italic>Methanospirillum hungatei<\/jats:italic> strains GP1 and SK, which is separate from the type strain JF-1<jats:sup>T<\/jats:sup>. Cultivation-independent surveys indicate the occurrence of this phylogenetic group in sediments and anaerobic digesters. The abundance of this clade appears to be negatively affected by high nitrogen loads, indicating a sensitivity to certain nitrogen compounds that is not known in <jats:italic>M<\/jats:italic>. <jats:italic>hungatei<\/jats:italic> JF-1<jats:sup>T<\/jats:sup>. The relatively large core genome of this <jats:italic>Methanospirillum<\/jats:italic> clade is indicative of niche specialization and efficient control of horizontal gene transfer. Genes for nitrogenase and F<jats:sub>420<\/jats:sub>-dependent secondary alcohol dehydrogenase contribute to the metabolic versatility of this lineage. Characteristics of the new isolate such as the ability to utilize 2-propanol as an electron donor or the requirement for acetate as a carbon source are found also in the strains GP1 and SK, but not in the type strain <jats:italic>M<\/jats:italic>. <jats:italic>hungatei<\/jats:italic> JF-1<jats:sup>T<\/jats:sup>. Based on the genomic differences to related species, a new species within the genus <jats:italic>Methanospirillum<\/jats:italic> is proposed with the name <jats:italic>M<\/jats:italic>. <jats:italic>purgamenti<\/jats:italic> sp. nov. The determined phenotypic characteristics support this proposal and indicate a metabolic adaptation to a separate ecological niche.<\/jats:p>","DOI":"10.1371\/journal.pone.0308405","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T20:34:18Z","timestamp":1724704458000},"page":"e0308405","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Description and genome analysis of a novel archaeon isolated from a syntrophic pyrite-forming enrichment culture and reclassification of Methanospirillum hungatei strains GP1 and SK as Methanospirillum purgamenti sp. nov."],"prefix":"10.1371","volume":"19","author":[{"given":"Nathalie","family":"Pradel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"Bartoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Koenen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Bale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1641-019X","authenticated-orcid":true,"given":"Meina","family":"Neumann-Schaal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathrin","family":"Spr\u00f6er","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyke","family":"Bunk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Rohde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Pester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6247-0938","authenticated-orcid":true,"given":"Stefan","family":"Spring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"key":"pone.0308405.ref001","doi-asserted-by":"crossref","first-page":"6897","DOI":"10.1073\/pnas.1814412116","article-title":"Pyrite 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