{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:57:24Z","timestamp":1774324644123,"version":"3.50.1"},"reference-count":17,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T00:00:00Z","timestamp":1724112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012669","name":"Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission","doi-asserted-by":"publisher","award":["cstc2021jcyj-msxmX0763"],"award-info":[{"award-number":["cstc2021jcyj-msxmX0763"]}],"id":[{"id":"10.13039\/501100012669","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012232","name":"National Key Clinical Specialty Discipline Construction Program of China","doi-asserted-by":"publisher","award":["Obstetrics and Gynecology"],"award-info":[{"award-number":["Obstetrics and Gynecology"]}],"id":[{"id":"10.13039\/501100012232","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Postoperative nausea and vomiting are common complications after surgery, and female patients are more likely to experience these adverse events. The goal of this study was to explore the relationship between the <jats:italic>CHRM3 rs2165870<\/jats:italic> polymorphism and postoperative vomiting incidence in female patients who underwent laparoscopic surgery.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Two hundred female patients who underwent elective laparoscopic surgery with subsequent patient-controlled intravenous analgesia using dexmedetomidine and sufentanil were prospectively enrolled. The <jats:italic>CHRM3 rs2165870<\/jats:italic> and <jats:italic>KCNB2 rs349358<\/jats:italic> polymorphisms were genotyped using MassARRAY SNP typing technology. Demographic data and preoperative laboratory results of all patients were recorded. Postoperative analgesia-related information, incidence of postoperative nausea and vomiting, and other adverse events were followed up and recorded for analysis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>No significant differences were observed in any of the demographic characteristics of these patients among the different genotype carriers (<jats:italic>P<\/jats:italic>&gt;0.05). The percentages of patients with each genotype of <jats:italic>CHRM3<\/jats:italic> were 67% (GG), 28.5% (GA) and 4.5% (AA). We found that the AA or A allele of the <jats:italic>CHRM3 rs2165870<\/jats:italic> polymorphism elevated the risk of postoperative vomiting (AA versus GG; OR, 6.94; 95% CI, 1.49\u201332.46; <jats:italic>P<\/jats:italic> = 0.014; A versus G; OR, 2.52; 95% CI, 1.22\u20135.19; <jats:italic>P<\/jats:italic> = 0.012). The percentages of patients with each genotype of <jats:italic>KCNB2<\/jats:italic> were 84.5% (TT), 15.5% (CT) and 0% (CC). There were no significant differences in the postoperative nausea or vomiting rate across the <jats:italic>KCNB2 rs349358<\/jats:italic> polymorphisms (<jats:italic>P<\/jats:italic>&gt;0.05).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The <jats:italic>CHRM3 rs2165870<\/jats:italic> polymorphism is associated with the occurrence of postoperative vomiting in female patients who have undergone laparoscopic surgery. The AA genotype or A allele of the <jats:italic>CHRM3 rs2165870<\/jats:italic> polymorphism elevates the risk of postoperative vomiting.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Trial registration<\/jats:title>\n<jats:p><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.chictr.org.cn\/\" xlink:type=\"simple\">www.chictr.org.cn<\/jats:ext-link>, registration number: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/pesquisa.bvsalud.org\/global-literature-on-novel-coronavirus-2019-ncov\/resource\/en\/ictrp-ChiCTR2200062425\" xlink:type=\"simple\">ChiCTR2200062425<\/jats:ext-link>.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0309136","type":"journal-article","created":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T17:30:19Z","timestamp":1724175019000},"page":"e0309136","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["CHRM3 (rs2165870) gene polymorphism is related to postoperative vomiting in female patients undergoing laparoscopic surgery. Prospective observational study"],"prefix":"10.1371","volume":"19","author":[{"given":"Meng","family":"Cai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Gan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofeng","family":"Lei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8568-9166","authenticated-orcid":true,"given":"Jin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,8,20]]},"reference":[{"key":"pone.0309136.ref001","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1213\/00000539-199401000-00004","article-title":"The postoperative interview: assessing risk factors for nausea and vomiting","volume":"78","author":"M.M. Cohen","year":"1994","journal-title":"Anesthesia and analgesia"},{"key":"pone.0309136.ref002","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1001\/jama.287.10.1233","article-title":"Postoperative nausea and vomiting\u2014can it be eliminated?.","volume":"287","author":"T.J. Gan","year":"2002","journal-title":"JAMA"},{"key":"pone.0309136.ref003","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1093\/bja\/88.2.234","article-title":"Comparison of predictive models for postoperative nausea and vomiting","volume":"88","author":"C.C. Apfel","year":"2002","journal-title":"British journal of anaesthesia"},{"key":"pone.0309136.ref004","doi-asserted-by":"crossref","first-page":"110437","DOI":"10.1016\/j.jclinane.2021.110437","article-title":"Impact of opioid-free anaesthesia on postoperative nausea, vomiting and pain after gynaecological laparoscopy\u2014A randomised controlled trial","volume":"75","author":"C. Massoth","year":"2021","journal-title":"J Clin Anesth"},{"key":"pone.0309136.ref005","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1097\/ALN.0b013e31821810c7","article-title":"Genome-wide Association study using pooled DNA to identify candidate markers mediating susceptibility to postoperative nausea and vomiting","volume":"115","author":"P.K. Janicki","year":"2011","journal-title":"Anesthesiology"},{"key":"pone.0309136.ref006","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.bja.2018.02.025","article-title":"CHRM3 rs2165870 polymorphism is independently associated with postoperative nausea and vomiting, but combined prophylaxis is effective","volume":"121","author":"S. Klenke","year":"2018","journal-title":"British journal of anaesthesia"},{"key":"pone.0309136.ref007","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.accpm.2019.04.012","article-title":"Genetic contribution to PONV risk","volume":"39","author":"S. Klenke","year":"2020","journal-title":"Anaesth Crit Care Pain Med"},{"key":"pone.0309136.ref008","first-page":"319","article-title":"CHRM3 rs2165870 Polymorphism Correlates with Postoperative Nausea and Vomiting Incidence and the Efficacy of Ondansetron in a Chinese Han Population","volume":"13","author":"J. Wang","year":"2020","journal-title":"Pharmgenomics Pers Med"},{"key":"pone.0309136.ref009","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1097\/EJA.0000000000001224","article-title":"Genetic variability in postoperative nausea and vomiting: A systematic review","volume":"37","author":"S. Klenke","year":"2020","journal-title":"European journal of anaesthesiology"},{"key":"pone.0309136.ref010","doi-asserted-by":"crossref","first-page":"e210","DOI":"10.1016\/j.bja.2018.12.005","article-title":"Opioids in the postoperative period: a call for consensus","volume":"122","author":"M.P. Sebastian","year":"2019","journal-title":"British journal of anaesthesia"},{"key":"pone.0309136.ref011","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1213\/ane.0b013e31817b5ebb","article-title":"04\u201306 Study, \"A randomized, double-blind study to evaluate the efficacy and safety of three different doses of palonosetron versus placebo for preventing postoperative nausea and vomiting","volume":"107","author":"K.A. Candiotti","year":"2008","journal-title":"Anesthesia and analgesia"},{"key":"pone.0309136.ref012","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4097\/kja.d.18.00011","article-title":"Aprepitant in combination with palonosetron for the prevention of postoperative nausea and vomiting in female patients using intravenous patient-controlled analgesia","volume":"71","author":"J.H. Yoo","year":"2018","journal-title":"Korean J Anesthesiol"},{"key":"pone.0309136.ref013","doi-asserted-by":"crossref","first-page":"786105","DOI":"10.3389\/fphar.2021.786105","article-title":"\"Potential Role for Combined Subtype-Selective Targeting of M(1) and M(3) Muscarinic Receptors in Gastrointestinal and Liver Diseases.\"","volume":"12","author":"M. Tolaymat","year":"2021","journal-title":"Front Pharmacol"},{"key":"pone.0309136.ref014","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1067\/mai.2003.71","article-title":"Sequence variation in the promoter region of the cholinergic receptor muscarinic 3 gene and asthma and atopy","volume":"111","author":"J. Donfack","year":"2003","journal-title":"J Allergy Clin Immunol"},{"key":"pone.0309136.ref015","doi-asserted-by":"crossref","first-page":"3625","DOI":"10.2337\/db06-0379","article-title":"CHRM3 gene variation is associated with decreased acute insulin secretion and increased risk for early-onset type 2 diabetes in Pima Indians","volume":"55","author":"Y. Guo","year":"2006","journal-title":"Diabetes"},{"key":"pone.0309136.ref016","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/sj.bjp.0706780","article-title":"Muscarinic receptors: their distribution and function in body systems, and the implications for treating overactive bladder","volume":"148","author":"P. Abrams","year":"2006","journal-title":"British journal of pharmacology"},{"key":"pone.0309136.ref017","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1111\/j.1365-2982.2008.01089.x","article-title":"Effect of tolterodine on gastrointestinal transit and bowel habits in healthy subjects","volume":"20","author":"A.E. Bharucha","year":"2008","journal-title":"Neurogastroenterol Motil"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0309136","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T17:30:36Z","timestamp":1724175036000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0309136"}},"subtitle":[],"editor":[{"given":"Guilherme Antonio Moreira","family":"de Barros","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,8,20]]},"references-count":17,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0309136","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,20]]}}}