{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:53:00Z","timestamp":1789771980425,"version":"4.0.1"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Rond\u00f4nia de Amparo ao Desenvolvimento das A\u00e7\u00f5es Cient\u00edficas e Tecnol\u00f3gicas e \u00e0 Pesquisa do Estado de Rond\u00f4nia"},{"name":"National Institute of Science and Technology of Epidemiology of the Western Amazon","award":["#465657\/2014-1"],"award-info":[{"award-number":["#465657\/2014-1"]}]},{"name":"Fiocruz Rond\u00f4nia Research Excellence Program\u2014PROEP","award":["VPG-DI-008-FIO-21-2-21"],"award-info":[{"award-number":["VPG-DI-008-FIO-21-2-21"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["13\/2020"],"award-info":[{"award-number":["13\/2020"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programa de Apoio \u00e0 P\u00f3s-gradua\u00e7\u00e3o da Amaz\u00f4nia Legal","award":["002\/2021"],"award-info":[{"award-number":["002\/2021"]}]},{"name":"CNPq productivity fellows","award":["process number: 304830\/2022-4"],"award-info":[{"award-number":["process number: 304830\/2022-4"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Financial code 001."],"award-info":[{"award-number":["Financial code 001."]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Entomological investigations were conducted for the first time in urban forest remnants of Porto Velho, state of Rond\u00f4nia, Brazil, to explore the transmission dynamics of <jats:italic>Leishmania<\/jats:italic>. Sand fly collections were carried out at ten sites, encompassing both canopy and ground strata, from October to December 2021. A total of 1,671 sand flies were collected, representing 42 species within 12 genera. <jats:italic>Nyssomyia<\/jats:italic> Antunesi (n = 384) and <jats:italic>Psychodopygus davisi<\/jats:italic> (n = 111) were the most abundant species. Molecular analyses targeting the V7V8 region (18S gene) unveiled the presence of sequences 100% identical to <jats:italic>Leishmania infantum<\/jats:italic> in females of <jats:italic>Bichromomyia flaviscutellata<\/jats:italic> (1), <jats:italic>Nyssomyia<\/jats:italic> Antunesi complex (6), <jats:italic>Nyssomyia umbratilis<\/jats:italic> (1), <jats:italic>Nyssomyia<\/jats:italic> sp. (1), <jats:italic>Psychodopygus ayrozai<\/jats:italic> (1), <jats:italic>Ps<\/jats:italic>. <jats:italic>davisi<\/jats:italic> (3), <jats:italic>Psychodopygus paraensis<\/jats:italic> (1), and <jats:italic>Sciopemyia sordellii<\/jats:italic> (1). Sequences 100% similar to <jats:italic>Trypanosoma minasense<\/jats:italic> were found in two samples of the <jats:italic>Nyssomyia<\/jats:italic> Antunesi complex, and two samples of <jats:italic>Sc<\/jats:italic>. <jats:italic>sordellii<\/jats:italic> presented 100% identity to a <jats:italic>Trypanosoma<\/jats:italic> sp. strain, previously identified in this same sand fly in Rond\u00f4nia. Sequencing of <jats:italic>Cytb<\/jats:italic> fragment suggested <jats:italic>Homo sapiens<\/jats:italic>, <jats:italic>Dasypus novemcinctus<\/jats:italic> and <jats:italic>Tamandua tetradactyla<\/jats:italic> as the blood source for distinct sand flies. The identification of sequences similar to <jats:italic>L<\/jats:italic>. <jats:italic>infantum<\/jats:italic> in sand flies collected in urban forest fragments is noteworthy, correlating with the recent local and regional occurrence of autochthonous cases of human visceral leishmaniasis. However, further studies are imperative to ascertain the presence of hosts\/reservoirs and evaluate the risk of <jats:italic>L<\/jats:italic>. <jats:italic>infantum<\/jats:italic> transmission to humans.<\/jats:p>","DOI":"10.1371\/journal.pone.0309168","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T17:31:06Z","timestamp":1723829466000},"page":"e0309168","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Entomological inferences highlight the risk of Leishmania transmission in the urban area of Porto Velho, Rond\u00f4nia, Brazil"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5126-380X","authenticated-orcid":true,"given":"Michelli Santos","family":"da Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7543-168X","authenticated-orcid":true,"given":"Amanda Maria","family":"Picelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila","family":"Pereira de Fran\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunice Aparecida Bianchi","family":"Galati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Dilermando","family":"Andrade Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genimar Rebou\u00e7as","family":"Juli\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2799-8267","authenticated-orcid":true,"given":"Felipe","family":"Dutra-R\u00eago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jansen Fernandes de","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"pone.0309168.ref001","article-title":"A Historical Overview of the Classification, Evolution, and Dispersion of Leishmania Parasites and Sandflies.","author":"M Akhoundi","year":"2016","journal-title":"PLoS Neglected Tropical Diseases. 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