{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T22:49:07Z","timestamp":1791586147451,"version":"4.3.4"},"reference-count":25,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Compared with machine staining, traditional manual staining faces various problems, such as a low preparation success rate, low efficiency, and harm to the human body due to corrosive gases. Therefore, a stainer that is of low cost, has strong corrosion resistance, and is suitable for small-batch preparation should be developed. In this study, by choosing a rotary scheme as the structural basis, a reusable container cover and a master-slave manipulator cooperation scheme are developed, which greatly improve the space utilization rate. Through material selection and structural design, in the designed stainer, effective protection against strong acids with high volatility and permeability is realized, thereby eliminating the corrosion issue. Using the designed splitting-running algorithm for the dyeing procedure, simultaneous multistaining is realized, which significantly improves the staining efficiency. Compared with large-sized stainers, the cost of the proposed stainer is very low, which will help popularize early screening for cervical cancer in low- and middle-income countries.<\/jats:p>","DOI":"10.1371\/journal.pone.0309334","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T17:31:23Z","timestamp":1728581483000},"page":"e0309334","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design and implementation of corrosion-resistant multitasking cell stainers"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7859-8072","authenticated-orcid":true,"given":"Chengsheng","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Run","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libo","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"pone.0309334.ref001","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.soncn.2019.02.003","article-title":"Cervical cancer: an overview of pathophysiology and management","volume":"35","author":"CA Johnson","year":"2019","journal-title":"Seminars in oncology nursing"},{"key":"pone.0309334.ref002","doi-asserted-by":"crossref","first-page":"e197","DOI":"10.1016\/S2214-109X(22)00501-0","article-title":"Global estimates of incidence and mortality of cervical cancer in 2020: a baseline analysis of the WHO Global Cervical Cancer Elimination Initiative","volume":"11","author":"D Singh","year":"2023","journal-title":"Lancet Glob Health"},{"issue":"1","key":"pone.0309334.ref003","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.sxmr.2019.09.005","article-title":"Cervical cancer screening: past, present, and future","volume":"8","author":"SL Bedell","year":"2020","journal-title":"Sexual medicine reviews"},{"key":"pone.0309334.ref004","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ejca.2019.12.013","article-title":"Effect of organised cervical cancer screening on cervical cancer mortality in Europe: a systematic review","volume":"127","author":"EE Jansen","year":"2020","journal-title":"European Journal of Cancer"},{"issue":"5","key":"pone.0309334.ref005","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1053\/j.gastro.2018.07.017","article-title":"Effects of organized colorectal cancer screening on cancer incidence and mortality in a large community-based population","volume":"155","author":"TR Levin","year":"2018","journal-title":"Gastroenterology"},{"issue":"7","key":"pone.0309334.ref006","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1093\/annonc\/mdz117","article-title":"Prolonged lung cancer screening reduced 10-year mortality in the MILD trial: new confirmation of lung cancer screening efficacy","volume":"30","author":"U Pastorino","year":"2019","journal-title":"Annals of Oncology"},{"key":"pone.0309334.ref007","first-page":"153","article-title":"Cost-effectiveness and cost-benefit of cervical cancer screening with liquid based cytology compared with conventional cytology in Germany","author":"SF Armstrong","year":"2020","journal-title":"Annals of Oncology"},{"issue":"1","key":"pone.0309334.ref008","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12889-020-08688-4","article-title":"Cervical cancer screening program based on primary DNA-HPV testing in a Brazilian city: a cost-effectiveness study protocol","volume":"20","author":"JC Teixeira","year":"2020","journal-title":"BMC Public Health"},{"key":"pone.0309334.ref009","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jcv.2019.03.005","article-title":"Liquid biopsy of HPV DNA in cervical cancer","volume":"114","author":"TH Cheung","year":"2019","journal-title":"Journal of Clinical Virology"},{"key":"pone.0309334.ref010","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.3389\/fmicb.2019.03116","article-title":"Human papillomavirus E6 and E7: the cervical cancer hallmarks and targets for therapy","author":"A Pal","year":"2020","journal-title":"Frontiers in microbiology"},{"issue":"2","key":"pone.0309334.ref011","first-page":"1351","article-title":"Ki-67 and P16 proteins in cervical cancer and precancerous lesions of young women and the diagnostic value for cervical cancer and precancerous lesions","volume":"18","author":"Q Shi","year":"2019","journal-title":"Oncology letters"},{"issue":"2","key":"pone.0309334.ref012","first-page":"1351","article-title":"Ki-67 and P16 proteins in cervical cancer and precancerous lesions of young women and the diagnostic value for cervical cancer and precancerous lesions","volume":"18","author":"Y Zhu","year":"2019","journal-title":"Oncology letters"},{"key":"pone.0309334.ref013","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00138-020-01063-8","article-title":"ColpoNet for automated cervical cancer screening using colposcopy images","volume":"31","author":"SK Saini","year":"2020","journal-title":"Machine Vision and Applications"},{"key":"pone.0309334.ref014","article-title":"Investigation of the Clinical Application Value of HR-HPV DNA Combined with Liquid Based Cytology in Colposcopy of Cervical Cancer","author":"Y Zhou","year":"2022","journal-title":"Contrast Media & Molecular Imaging"},{"key":"pone.0309334.ref015","doi-asserted-by":"crossref","DOI":"10.1590\/1414-431x20187517","article-title":"Analysis of the application values of different combination schemes of liquid-based cytology and high-risk human papilloma virus test in the screening of high-grade cervical lesions","volume":"52","author":"J Wang","year":"2019","journal-title":"Brazilian Journal of Medical and Biological Research"},{"issue":"2","key":"pone.0309334.ref016","doi-asserted-by":"crossref","first-page":"92","DOI":"10.14715\/cmb\/2017.63.2.14","article-title":"The clinical research of Thinprep Cytology Test (TCT) combined with HPV-DNA detection in screening cervical cancer","volume":"63","author":"Y Liu","year":"2017","journal-title":"Cellular and Molecular Biology"},{"key":"pone.0309334.ref017","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/B978-081551500-5.50029-X","volume-title":"Handbook of Environmental Degradation of Materials","author":"WS Tait","year":"2005"},{"key":"pone.0309334.ref018","doi-asserted-by":"crossref","unstructured":"Huang Z, Feng C, Wang J, Liu W, Xie Y. Research on metal corrosion mechanism and inhibition measures of transmission and distribution equipment in Hunan. IOP Conference Series: Earth and Environmental Science.2021;012035.","DOI":"10.1088\/1755-1315\/621\/1\/012035"},{"issue":"8","key":"pone.0309334.ref019","doi-asserted-by":"crossref","first-page":"085207","DOI":"10.1088\/2053-1591\/ab2222","article-title":"The effect of PTFE addition on mechanical and anti-corrosion properties of coating of heat exchangers","volume":"6","author":"C Yanhai","year":"2019","journal-title":"Materials Research Express"},{"key":"pone.0309334.ref020","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.porgcoat.2017.02.009","article-title":"Polyaniline\/polyvinyl chloride blended coatings for the corrosion protection of carbon steel","volume":"106","author":"RM Bandeira","year":"2017","journal-title":"Progress in Organic Coatings"},{"issue":"8","key":"pone.0309334.ref021","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1177\/0892705719889095","article-title":"Review of natural fiber-reinforced engineering plastic composites, their applications in the transportation sector and processing techniques","volume":"35","author":"V Chauhan","year":"2022","journal-title":"Journal of Thermoplastic Composite Materials"},{"key":"pone.0309334.ref022","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1007\/s11665-020-04759-0","article-title":"Friction and wear behaviors of kevlar\/polytetrafluoroethylene braided composite in oscillatory contacts","volume":"29","author":"J Liu","year":"2020","journal-title":"Journal of Materials Engineering and Performance"},{"issue":"2","key":"pone.0309334.ref023","first-page":"58","article-title":"Investigation Of The Use Of Teflon (Ptfe), Lead, Brass And Carbon Fiber Plates As Friction Pads In Rotational Friction Dampers","volume":"21","author":"E Suci","year":"2022","journal-title":"Selcuk University Journal of Engineering Sciences"},{"key":"pone.0309334.ref024","doi-asserted-by":"crossref","first-page":"105032","DOI":"10.1016\/j.mtcomm.2022.105032","article-title":"Effect of molybdenum content on corrosion resistance and corrosion behavior of Ti-Mo titanium alloy in hydrochloric acid","volume":"34","author":"H Zhao","year":"2023","journal-title":"Materials Today Communications"},{"key":"pone.0309334.ref025","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1515\/corrrev-2019-0029","article-title":"Corrosion behavior of titanium alloys in acidic and saline media: Role of alloy design","volume":"38","author":"MO Bodunrin","year":"2020","journal-title":"Corrosion Reviews"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0309334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T17:31:38Z","timestamp":1728581498000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0309334"}},"subtitle":[],"editor":[{"given":"Shih-Chao","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,10,10]]},"references-count":25,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0309334","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,10]]}}}