{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:33:47Z","timestamp":1792024427371,"version":"4.4.0"},"reference-count":68,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T00:00:00Z","timestamp":1726790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Research Initiation Funding of Zhejiang Sci-Tech University","award":["No. 19042112-Y"],"award-info":[{"award-number":["No. 19042112-Y"]}]},{"name":"National Support Funding for Reform and Development of Local Universities","award":["No. 303013-2021-0007"],"award-info":[{"award-number":["No. 303013-2021-0007"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Fritillariae thunbergii<\/jats:italic> Bulbus (FTB) is a traditional Chinese medicine that has been widely cultivated for its expectorant, antitussive, antiasthmatic, antiviral, and anticancer properties. The yield and quality of <jats:italic>F<\/jats:italic>. <jats:italic>thunbergii<\/jats:italic> are influenced by cultivation conditions, such as the use of fertilizers. However, the optimal type of fertilizers for maximum quality and yield and underlying mechanisms are not clear. We collected <jats:italic>F<\/jats:italic>. <jats:italic>thunbergii<\/jats:italic> using raw chicken manure (RC), organic fertilizer (OF), and plant ash (PA) as the base fertilizer in Pan\u2019an County, Jinhua City, Zhejiang Province as experimental materials. The combined results of HPLC-ELSD detection and yield statistics showed that the <jats:italic>F<\/jats:italic>. <jats:italic>thunbergii<\/jats:italic> with OF application was the best, with the content of peimine and peiminine reaching 0.0603% and 0.0502%, respectively. In addition, the yield was 2.70 kg\/m<jats:sup>2<\/jats:sup>. Transcriptome analysis indicated that up-regulation of the ABA signaling pathway might promote bulb yield. Furthermore, putative key genes responsible for steroidal alkaloid accumulation were identified. These results provided guiding significance for the rational fertilization conditions of <jats:italic>F<\/jats:italic>. <jats:italic>thunbergii<\/jats:italic> as well as the basis for the exploration of functional genes related to the alkaloid biosynthesis pathway.<\/jats:p>","DOI":"10.1371\/journal.pone.0309978","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T17:22:08Z","timestamp":1726852928000},"page":"e0309978","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomic analysis reveals effects of fertilization towards growth and quality of Fritillariae thunbergii bulbus"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3455-787X","authenticated-orcid":true,"given":"Luman","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7194-124X","authenticated-orcid":true,"given":"Hongwei","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6952-1096","authenticated-orcid":true,"given":"Zhuoheng","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,9,20]]},"reference":[{"key":"pone.0309978.ref001","article-title":"Fritillaria thunbergii Miq. (Zhe Beimu): A review on its traditional uses, phytochemical profile and pharmacological properties","volume":"153","author":"SH Nile","year":"2021","journal-title":"Food and Chemical Toxicology"},{"issue":"4","key":"pone.0309978.ref002","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/S1875-5364(13)60050-3","article-title":"Phytochemical and biological research of Fritillaria medicine resources","volume":"11","author":"DC Hao","year":"2013","journal-title":"Chinese Journal of Natural Medicines"},{"key":"pone.0309978.ref003","first-page":"126","volume-title":"Pharmacopoeia of People\u2019s Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2020"},{"key":"pone.0309978.ref004","doi-asserted-by":"crossref","first-page":"985935","DOI":"10.3389\/fphar.2022.985935","article-title":"Efficacy, chemical composition, and pharmacological effects of herbal drugs derived from Fritillaria cirrhosa D. Don and Fritillaria thunbergii Miq","volume":"13","author":"F Wu","year":"2022","journal-title":"Front Pharmacol"},{"issue":"1\u20133","key":"pone.0309978.ref005","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ejphar.2006.08.066","article-title":"Selective antagonism activity of alkaloids from bulbs Fritillariae at muscarinic receptors: functional studies","volume":"551","author":"BQ Lin","year":"2006","journal-title":"European journal of pharmacology"},{"issue":"03","key":"pone.0309978.ref006","first-page":"315","article-title":"Multidrug resistance reversal activity of total alkaloid from Fritillaria thunbergii on cisplatin-resistant human lung adenocarcinoma A549\/DDP cells","volume":"27","author":"ZH Li","year":"2013","journal-title":"Chinese Journal of Pharmacology and Toxicology"},{"issue":"7","key":"pone.0309978.ref007","article-title":"Fritillariae Thunbergii Bulbus: Traditional Uses, Phytochemistry, Pharmacodynamics, Pharmacokinetics and Toxicity","volume":"20","author":"H Li","year":"2019","journal-title":"International Journal of Molecular Sciences"},{"issue":"5","key":"pone.0309978.ref008","first-page":"925","article-title":"Two new steroidal alkaloids from the bulbs of Fritillaria thunbergii","volume":"96","author":"WS Suh","year":"2018","journal-title":"Heterocycles"},{"issue":"12","key":"pone.0309978.ref009","first-page":"1755","article-title":"Study on the alkaloid content and synthesis related gene expression of Fritillaria thunbergii from different regions","volume":"40","author":"QL Wu","year":"2020","journal-title":"Guangxi Botanical Journal"},{"issue":"05","key":"pone.0309978.ref010","first-page":"576","article-title":"The effects of different fertilization and coverage measures on the yield and effective components of Fritillaria thunbergii","volume":"20","author":"X Lu","year":"2018","journal-title":"Modern Chinese Medicine"},{"issue":"6","key":"pone.0309978.ref011","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1007\/s00438-021-01821-x","article-title":"Transcriptome repository of North-Western Himalayan endangered medicinal herbs: a paramount approach illuminating molecular perspective of phytoactive molecules and secondary metabolism","volume":"296","author":"B Kapoor","year":"2021","journal-title":"Molecular Genetics And Genomics"},{"issue":"6","key":"pone.0309978.ref012","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.plaphy.2004.05.012","article-title":"New aspects of sterol biosynthesis in growth and development of higher plants","volume":"42","author":"H. Schaller","year":"2004","journal-title":"Plant Physiology and Biochemistry"},{"key":"pone.0309978.ref013","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.pep.2016.10.002","article-title":"cDNA isolation and functional characterization of squalene synthase gene from Ornithogalum caudatum","volume":"130","author":"M Liu","year":"2017","journal-title":"Protein Expression and Purification"},{"issue":"1","key":"pone.0309978.ref014","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1186\/s12870-020-02411-6","article-title":"Identification and analysis of CYP450 and UGT supergene family members from the transcriptome of Aralia elata (Miq.) seem reveal candidate genes for triterpenoid saponin biosynthesis","volume":"20","author":"Y Cheng","year":"2020","journal-title":"BMC Plant Biology"},{"key":"pone.0309978.ref015","volume-title":"Genetics and Genomics of High-Altitude Crops","author":"G Vijay","year":"2024","edition":"1"},{"issue":"12","key":"pone.0309978.ref016","doi-asserted-by":"crossref","first-page":"7315","DOI":"10.1007\/s00344-023-11018-8","article-title":"Transcriptomic and metabolomic reprogramming to explore the High-Altitude adaptation of medicinal plants: A Review","volume":"42","author":"V Kumar","year":"2023","journal-title":"Journal of Plant Growth Regulation"},{"key":"pone.0309978.ref017","doi-asserted-by":"crossref","first-page":"117541","DOI":"10.1016\/j.indcrop.2023.117541","article-title":"In vitro propagation and omics breakthroughs for understanding specialized metabolite production in high-value Himalayan Fritillaria species","volume":"205","author":"S Sharma","year":"2023","journal-title":"Industrial Crops and Products"},{"issue":"15","key":"pone.0309978.ref018","first-page":"2946","article-title":"Effect of different fertilization treatments on yield and secondary metabolites of Codonopsis pilosula","volume":"42","author":"JD Hu","year":"2017","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"issue":"10","key":"pone.0309978.ref019","first-page":"2015","article-title":"Effects of Chemical Fertilizers and Organic Fertilizer on Yield of Ligusticum chuanxiong Rhizome","volume":"38","author":"Q Liang","year":"2015","journal-title":"Zhong Yao Cai"},{"issue":"4","key":"pone.0309978.ref020","doi-asserted-by":"crossref","first-page":"e0266190","DOI":"10.1371\/journal.pone.0266190","article-title":"Effect of chicken manure and chemical fertilizer on the yield and qualities of white mugwort at dissimilar harvesting times","volume":"17","author":"O Thepsilvisut","year":"2022","journal-title":"PLoS One"},{"issue":"7","key":"pone.0309978.ref021","first-page":"410","article-title":"Corset: enabling differential gene expression analysis for de novo assembled transcriptomes","volume":"15","author":"NM Davidson","year":"2014","journal-title":"Genome Biology"},{"issue":"3","key":"pone.0309978.ref022","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1093\/molbev\/msx319","article-title":"BUSCO Applications from Quality Assessments to Gene Prediction and Phylogenomics","volume":"35","author":"RM Waterhouse","year":"2018","journal-title":"Molecular Biology and Evolution"},{"key":"pone.0309978.ref023","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/1471-2105-12-323","article-title":"RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome","volume":"12","author":"B Li","year":"2011","journal-title":"BMC Bioinformatics"},{"issue":"7","key":"pone.0309978.ref024","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/nmeth.1226","article-title":"Mapping and quantifying mammalian transcriptomes by RNA-Seq","volume":"5","author":"A Mortazavi","year":"2008","journal-title":"Nature Methods"},{"issue":"1","key":"pone.0309978.ref025","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes","volume":"28","author":"M Kanehisa","year":"2000","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"pone.0309978.ref026","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/bioinformatics\/btp612","article-title":"DEGseq: an R package for identifying differentially expressed genes from RNA-seq data","volume":"26","author":"L Wang","year":"2010","journal-title":"Bioinformatics"},{"issue":"4","key":"pone.0309978.ref027","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"5","key":"pone.0309978.ref028","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.devcel.2010.10.010","article-title":"Integration of brassinosteroid signal transduction with the transcription network for plant growth regulation in Arabidopsis","volume":"19","author":"Y Sun","year":"2010","journal-title":"Developmental Cell"},{"key":"pone.0309978.ref029","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s12870-016-0771-y","article-title":"Plant hormone-mediated regulation of stress responses","volume":"16","author":"V Verma","year":"2016","journal-title":"BMC Plant Biology"},{"issue":"2","key":"pone.0309978.ref030","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/s12038-013-9302-2","article-title":"Plant innate immunity: An updated insight into defense mechanism","volume":"38","author":"M Muthamilarasan","year":"2013","journal-title":"Journal of Biosciences"},{"issue":"Pt 15","key":"pone.0309978.ref031","doi-asserted-by":"crossref","first-page":"3033","DOI":"10.1242\/jcs.115.15.3033","article-title":"mRNA surveillance: the perfect persist","volume":"115","author":"E Wagner","year":"2002","journal-title":"Journal of cell science"},{"issue":"6","key":"pone.0309978.ref032","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.cytogfr.2013.07.002","article-title":"Dynamic regulation of innate immunity by ubiquitin and ubiquitin-like proteins","volume":"24","author":"X Liu","year":"2013","journal-title":"Cytokine & Growth Factor Reviews"},{"issue":"1","key":"pone.0309978.ref033","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/cdd.2012.72","article-title":"Ubiquitination and selective autophagy","volume":"20","author":"S Shaid","year":"2013","journal-title":"Cell Death and Differentiation"},{"key":"pone.0309978.ref034","doi-asserted-by":"crossref","first-page":"16205","DOI":"10.1038\/nplants.2016.205","article-title":"Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism","volume":"3","author":"PD Sonawane","year":"2016","journal-title":"Nature Plants"},{"issue":"4","key":"pone.0309978.ref035","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.cell.2009.01.019","article-title":"The many pathways of RNA degradation","volume":"136","author":"J Houseley","year":"2009","journal-title":"Cell"},{"issue":"3","key":"pone.0309978.ref036","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1074\/jbc.275.3.1723","article-title":"ABFs, a family of ABA-responsive element binding factors","volume":"275","author":"H Choi","year":"2000","journal-title":"Journal of Biological Chemistry"},{"issue":"9","key":"pone.0309978.ref037","doi-asserted-by":"crossref","first-page":"3763","DOI":"10.1105\/tpc.114.130096","article-title":"Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato","volume":"26","author":"S Sawai","year":"2014","journal-title":"Plant Cell"},{"issue":"6","key":"pone.0309978.ref038","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1111\/tpj.12871","article-title":"Elucidating steroid alkaloid biosynthesis in Veratrum californicum: production of verazine in Sf9 cells","volume":"82","author":"MM Augustin","year":"2015","journal-title":"The Plant Journal"},{"issue":"20","key":"pone.0309978.ref039","doi-asserted-by":"crossref","first-page":"3418","DOI":"10.1002\/anie.200906967","article-title":"Cyclopamine and hedgehog signaling: chemistry, biology, medical perspectives","volume":"49","author":"P Heretsch","year":"2010","journal-title":"Angewandte Chemie International Edition"},{"issue":"4","key":"pone.0309978.ref040","doi-asserted-by":"crossref","first-page":"e13755","DOI":"10.1111\/ppl.13755","article-title":"Transcriptome analysis reveals candidate genes related to steroid alkaloid biosynthesis in Fritillaria anhuiensis","volume":"174","author":"T Shan","year":"2022","journal-title":"Physiologia Plantarum"},{"key":"pone.0309978.ref041","doi-asserted-by":"crossref","first-page":"113409","DOI":"10.1016\/j.indcrop.2021.113409","article-title":"Estimating Fritillaria thunbergii Miq. yield, quality, and potassium use efficiency in response to potassium application rate","volume":"164","author":"N Sui","year":"2021","journal-title":"Industrial Crops and Products"},{"issue":"1","key":"pone.0309978.ref042","doi-asserted-by":"crossref","first-page":"100003","DOI":"10.1016\/j.ccmp.2021.100003","article-title":"Screening of the optimal potassium fertilization rate for maximizing bulb yield and potassium nutrient evaluation in Fritillaria thunbergii miq","volume":"1","author":"L Wang","year":"2021","journal-title":"Clinical Complementary Medicine and Pharmacology"},{"issue":"6","key":"pone.0309978.ref043","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1007\/s11103-016-0503-6","article-title":"Physiological impacts of ABA-JA interactions under water-limitation","volume":"91","author":"C de Ollas","year":"2016","journal-title":"Plant Molecular Biology"},{"issue":"4","key":"pone.0309978.ref044","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1111\/j.1365-313X.2009.04092.x","article-title":"AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation","volume":"61","author":"T Yoshida","year":"2010","journal-title":"The Plant Journal"},{"issue":"01","key":"pone.0309978.ref045","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3724\/SP.J.1006.2017.00141","article-title":"Regulation on contents of endogenous hormones and asr1 gene expression of Maize seedling by exogenous ABA under low-temperature stress","volume":"43","author":"XY Li","year":"2017","journal-title":"Acta Agronomica Sinica"},{"issue":"1","key":"pone.0309978.ref046","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/tpj.13460","article-title":"Analysis of plant hormone profiles in response to moderate dehydration stress","volume":"90","author":"K Urano","year":"2017","journal-title":"The Plant Journal"},{"issue":"12","key":"pone.0309978.ref047","doi-asserted-by":"crossref","first-page":"3460","DOI":"10.1105\/tpc.104.025833","article-title":"Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis","volume":"16","author":"JP Anderson","year":"2004","journal-title":"Plant Cell"},{"issue":"1","key":"pone.0309978.ref048","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/jipb.13028","article-title":"Ethylene signaling in rice and Arabidopsis: New regulators and mechanisms","volume":"63","author":"H Zhao","year":"2021","journal-title":"Journal of Integrative Plant Biology"},{"issue":"7","key":"pone.0309978.ref049","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.molp.2018.05.001","article-title":"The antagonistic action of abscisic acid and cytokinin signaling mediates drought stress response in Arabidopsis","volume":"11","author":"X Huang","year":"2018","journal-title":"Molecular Plant"},{"issue":"1","key":"pone.0309978.ref050","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1186\/s12864-022-08724-0","article-title":"Integrative analysis of the steroidal alkaloids distribution and biosynthesis of bulbs Fritillariae Cirrhosae through metabolome and transcriptome analyses","volume":"23","author":"Q Lu","year":"2022","journal-title":"BMC Genomics"},{"issue":"1","key":"pone.0309978.ref051","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1104\/pp.124.1.95","article-title":"Analysis of the expression of CLA1, a gene that encodes the 1-deoxyxylulose 5-phosphate synthase of the 2-C-methyl-D-erythritol-4-phosphate pathway in Arabidopsis","volume":"124","author":"JM Est\u00e9vez","year":"2000","journal-title":"Plant Physiology"},{"issue":"4","key":"pone.0309978.ref052","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1104\/pp.003798","article-title":"Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway","volume":"129","author":"L Carretero-Paulet","year":"2002","journal-title":"Plant Physiology"},{"key":"pone.0309978.ref053","doi-asserted-by":"crossref","first-page":"112631","DOI":"10.1016\/j.phytochem.2020.112631","article-title":"Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.)\u2014High value Himalayan medicinal herb","volume":"183","author":"P Kumar","year":"2021","journal-title":"Phytochemistry"},{"issue":"2","key":"pone.0309978.ref054","first-page":"231","article-title":"The DWF4 gene of Arabidopsis encodes a cytochromecpha-hydroxylation steps in brassinosteroid biosynthesis","volume":"10","author":"S Choe","year":"1998","journal-title":"Plant Cell"},{"issue":"5","key":"pone.0309978.ref055","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1111\/j.1365-313X.2005.02639.x","article-title":"Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols","volume":"45","author":"S Fujita","year":"2006","journal-title":"The Plant Journal"},{"issue":"1","key":"pone.0309978.ref056","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nbt1173","article-title":"Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice","volume":"24","author":"T Sakamoto","year":"2006","journal-title":"Nature Biotechnology"},{"issue":"9","key":"pone.0309978.ref057","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1271\/bbb.60034","article-title":"CYP724B2 and CYP90B3 function in the early C-22 hydroxylation steps of brassinosteroid biosynthetic pathway in tomato","volume":"70","author":"T Ohnishi","year":"2006","journal-title":"Bioscience, Biotechnology and Biochemistry"},{"issue":"4","key":"pone.0309978.ref058","doi-asserted-by":"crossref","first-page":"2458","DOI":"10.1104\/pp.16.00137","article-title":"Two cytochrome P450 monooxygenases catalyze early hydroxylation steps in the potato steroid glycoalkaloid biosynthetic pathway","volume":"171","author":"N Umemoto","year":"2016","journal-title":"Plant Physiology"},{"issue":"1\u20132","key":"pone.0309978.ref059","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s11103-010-9663-y","article-title":"Arabidopsis CYP72C1 is an atypical cytochrome P450 that inactivates brassinosteroids","volume":"74","author":"LE Thornton","year":"2010","journal-title":"Plant Molecular Biology"},{"issue":"17","key":"pone.0309978.ref060","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1016\/j.phytochem.2006.05.042","article-title":"Tomato cytochrome P450 CYP734A7 functions in brassinosteroid catabolism","volume":"67","author":"T Ohnishi","year":"2006","journal-title":"Phytochemistry"},{"issue":"1","key":"pone.0309978.ref061","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-313X.2011.04567.x","article-title":"Rice CYP734As function as multisubstrate and multifunctional enzymes in brassinosteroid catabolism","volume":"67","author":"T Sakamoto","year":"2011","journal-title":"The Plant Journal"},{"issue":"6142","key":"pone.0309978.ref062","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1126\/science.1240230","article-title":"Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes","volume":"341","author":"M Itkin","year":"2013","journal-title":"Science"},{"key":"pone.0309978.ref063","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1016\/S0031-9422(00)85769-6","article-title":"11-Deoxojervine as a precursor for jervine biosynthesis in Veratrum grandiflorum","volume":"9","author":"K Kaneko","year":"1970","journal-title":"Phytochemistry"},{"issue":"6","key":"pone.0309978.ref064","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1093\/pcp\/pcx067","article-title":"Cytochrome P450 monooxygenase CYP716A141 is a unique b-Amyrin C-16b oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus (vol 57, pg 874, 2017)","volume":"58","author":"K Tamura","year":"2017","journal-title":"Plant and Cell Physiology"},{"key":"pone.0309978.ref065","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1073\/pnas.1323369111","article-title":"Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16\u03b1 hydroxylase from Bupleurum falcatum","volume":"111","author":"T Moses","year":"2014","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"3","key":"pone.0309978.ref066","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1104\/pp.104.055715","article-title":"Differential expression and evolution of the Arabidopsis CYP86A subfamily","volume":"137","author":"H Duan","year":"2005","journal-title":"Plant Physiology"},{"issue":"1","key":"pone.0309978.ref067","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1104\/pp.17.00501","article-title":"A dioxygenase catalyzes steroid 16\u03b1-hydroxylation in steroidal glycoalkaloid biosynthesis","volume":"175","author":"M Nakayasu","year":"2017","journal-title":"Plant Physiology"},{"issue":"12","key":"pone.0309978.ref068","doi-asserted-by":"crossref","first-page":"2062","DOI":"10.1093\/pcp\/pcr150","article-title":"The Cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng","volume":"52","author":"JY Han","year":"2011","journal-title":"Plant and Cell Physiology"}],"container-title":["PLOS 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