{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:34:06Z","timestamp":1791689646041,"version":"4.3.4"},"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100013987","name":"Government of Kenya","doi-asserted-by":"crossref","award":["KEMRI\/IRG\/EC0012"],"award-info":[{"award-number":["KEMRI\/IRG\/EC0012"]}],"id":[{"id":"10.13039\/100013987","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Carbapenem-resistant bacteria (CRB) present a significant global public health concern. Sub-Saharan Africa has borne a heavy burden of CRB with a reported prevalence of up to 60% in some patient populations. es in Africa focus on clinical CRB isolates, with limited data on their spread in the natural environment. Therefore, the purpose of this study was to report the recovery of CRB from Nairobi River surface waters and nearby anthropogenic and zoonotic sources in Nairobi County, Kenya. A total of 336 CRB were recovered from 336 (250 mL) samples, with 230 of the samples (68.5%) producing one or more CRB isolates. CRB were recovered most commonly from untreated sewage influent (100% of 36 samples; 79 total isolates), treated effluent (93% of 118 samples; 116 total isolates), Nairobi River surface waters upstream (100% of 36 samples; 57 total isolates), downstream (100% of 36 samples; 45 total isolates), and way downstream from the wastewater treatment plant (73% of 11 samples; 19 total isolates), slaughterhouse effluent discharges 1.5%, (5\/336), animal contact areas 0.9%, (3\/336), a manhole sewer from the affluent neighborhood of Karen at 2.7%, (9\/336) respectively. The CRB included\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (158, 47%),\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (74, 22%), and\n                    <jats:italic>Enterobacter spp<\/jats:italic>\n                    (43, 13%).\n                    <jats:italic>Aeromonas spp<\/jats:italic>\n                    (29, 9%)\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    (12, 3.6%),\n                    <jats:italic>Citrobacter freundii<\/jats:italic>\n                    (7, 2.1%),\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    (5, 1.5%) and other species (8, 2.4%). CRB genotypes included\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM<\/jats:sub>\n                    (246, 73.2%),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    (40, 12%),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>VIM<\/jats:sub>\n                    (51, 15.2%),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-48-like<\/jats:sub>\n                    (65, 19.3%),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>IMP<\/jats:sub>\n                    (15, 4.5%), and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>GES<\/jats:sub>\n                    (7, 2.1%). Sixty-nine of the CRB isolates (20.5%) harbored multiple carbapenemase-encoding genes. Our results indicate that clinically important CRB are commonly present in Nairobi River surface water and from nearby wastewater and livestock sources. These pose an important public health threat that requires urgent intervention strategies and additional investigation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0310026","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T13:44:33Z","timestamp":1731591873000},"page":"e0310026","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Carbapenemase-producing bacteria recovered from Nairobi River, Kenya surface water and from nearby anthropogenic and zoonotic sources"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6100-9090","authenticated-orcid":true,"given":"Rael J.","family":"Too","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel M.","family":"Kariuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George C.","family":"Gitao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilly C.","family":"Bebora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9216-1308","authenticated-orcid":true,"given":"Dixie F.","family":"Mollenkopf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Wittum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"issue":"1","key":"pone.0310026.ref001","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3343\/alm.2022.42.1.36","article-title":"Genotypic Distribution and Antimicrobial Susceptibilities of Carbapenemase-Producing Enterobacteriaceae Isolated From Rectal and Clinical Samples in Korean University Hospitals Between 2016 and 2019.","volume":"42","author":"S Jeong","year":"2022","journal-title":"Ann Lab Med [Internet]."},{"key":"pone.0310026.ref002","unstructured":"Centers for Disease Control and Prevention (U.S.). Antibiotic resistance threats in the United States, 2019 [Internet]. Centers for Disease Control and Prevention (U.S.); 2019 Nov [cited 2024 Jun 23]. Available from: https:\/\/stacks.cdc.gov\/view\/cdc\/82532"},{"key":"pone.0310026.ref003","volume-title":"WHO report on surveillance of antibiotic consumption: 2016\u20132018 early implementation","author":"World Health Organization","year":"2018"},{"issue":"1","key":"pone.0310026.ref004","doi-asserted-by":"crossref","first-page":"32","DOI":"10.4103\/2229-516X.198520","article-title":"Carbapenemase-producing Enterobacteriaceae: Risk factors for infection and impact of resistance on outcomes.","volume":"7","author":"S Mariappan","year":"2017","journal-title":"Int J App Basic Med Res [Internet]."},{"issue":"2","key":"pone.0310026.ref005","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3390\/antibiotics11020281","article-title":"Quantitative Risk Assessment for the Introduction of Carbapenem-Resistant Enterobacteriaceae (CPE) into Dutch Livestock Farms.","volume":"11","author":"N Dankittipong","year":"2022","journal-title":"Antibiotics [Internet]."},{"issue":"2","key":"pone.0310026.ref006","doi-asserted-by":"crossref","first-page":"O132","DOI":"10.1111\/1469-0691.12336","article-title":"KPC-2 and OXA-48 carbapenemase-harbouring Enterobacteriaceae detected in an Austrian wastewater treatment plant","volume":"20","author":"H Galler","year":"2014","journal-title":"Clinical Microbiology and Infection"},{"issue":"1","key":"pone.0310026.ref007","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s13756-017-0219-7","article-title":"Fecal carriage of extended-spectrum \u03b2-lactamase- and carbapenemase-producing Enterobacteriaceae in Egyptian patients with community-onset gastrointestinal complaints: a hospital -based cross-sectional study.","volume":"6","author":"HM Abdallah","year":"2017","journal-title":"Antimicrob Resist Infect Control"},{"key":"pone.0310026.ref008","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.2147\/IDR.S211746","article-title":"Emergence of two Escherichia coli strains co-harboring mcr-1 and blaNDM in fresh vegetables from China.","volume":"12","author":"BT Liu","year":"2019","journal-title":"IDR"},{"issue":"6","key":"pone.0310026.ref009","first-page":"e02338","article-title":"Novel Carbapenemases FLC-1 and IMI-2 Encoded by an Enterobacter cloacae Complex Isolated from Food Products","volume":"63","author":"MSM Brouwer","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"pone.0310026.ref010","doi-asserted-by":"crossref","first-page":"e01298","DOI":"10.1128\/AAC.01298-16","article-title":"Carbapenemase-Producing Enterobacteriaceae Recovered from the Environment of a Swine Farrow-to-Finish Operation in the United States","volume":"61","author":"DF Mollenkopf","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0310026.ref011","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.vetmic.2017.06.010","article-title":"Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows","volume":"207","author":"T He","year":"2017","journal-title":"Veterinary Microbiology"},{"issue":"1","key":"pone.0310026.ref012","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/jac\/dkx335","article-title":"Emergence of Escherichia coli co-producing NDM-1 and KPC-2 carbapenemases from a retail vegetable, China","volume":"73","author":"J Wang","year":"2018","journal-title":"Journal of Antimicrobial Chemotherapy"},{"issue":"12","key":"pone.0310026.ref013","doi-asserted-by":"crossref","first-page":"e01161","DOI":"10.1128\/AAC.01161-18","article-title":"Enterobacter cloacae Complex Sequence Type 171 Isolates Expressing KPC-4 Carbapenemase Recovered from Canine Patients in Ohio","volume":"62","author":"JB Daniels","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"pone.0310026.ref014","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1177\/10406387211065501","article-title":"Characterization of 2 Klebsiella pneumoniae carbapenemase\u2013producing Enterobacterales isolated from canine rectal swabs","volume":"34","author":"SD Cole","year":"2022","journal-title":"J VET Diagn Invest"},{"issue":"12","key":"pone.0310026.ref015","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/j.cmi.2018.04.004","article-title":"Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review","volume":"24","author":"R K\u00f6ck","year":"2018","journal-title":"Clinical Microbiology and Infection"},{"key":"pone.0310026.ref016","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.envpol.2017.05.095","article-title":"Abundance of carbapenemase genes (blaKPC, blaNDM and blaOXA-48) in wastewater effluents from Tunisian hospitals.","volume":"229","author":"E Nasri","year":"2017","journal-title":"Environmental Pollution"},{"issue":"6","key":"pone.0310026.ref017","doi-asserted-by":"crossref","first-page":"e0218650","DOI":"10.1371\/journal.pone.0218650","article-title":"Carbapenemase-producing Enterobacteriaceae and Aeromonas spp. present in wastewater treatment plant effluent and nearby surface waters in the US","volume":"14","author":"DA Mathys","year":"2019","journal-title":"PLoS ONE [Internet]."},{"issue":"13","key":"pone.0310026.ref018","doi-asserted-by":"crossref","first-page":"e00465","DOI":"10.1128\/aem.00465-22","article-title":"from Urban and Suburban Environments with Cephalosporinase- and Carbapenemase-Producing Enterobacterales. Elkins CA, editor","volume":"88","author":"GA Ballash","year":"2022","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"pone.0310026.ref019","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s13756-020-00736-3","article-title":"Emergence of \u03b2-lactamase- and carbapenemase- producing Enterobacteriaceae at integrated fish farms.","volume":"9","author":"D Hamza","year":"2020","journal-title":"Antimicrob Resist Infect Control"},{"issue":"9","key":"pone.0310026.ref020","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1128\/AEM.00054-13","article-title":"Characteristics of Extended-Spectrum \u03b2-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolates from Rivers and Lakes in Switzerland","volume":"79","author":"K Zurfluh","year":"2013","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"pone.0310026.ref021","doi-asserted-by":"crossref","first-page":"174","DOI":"10.3390\/pathogens8040174","article-title":"Environmental Prevalence of Carbapenem Resistance Enterobacteriaceae (CRE) in a Tropical Ecosystem in India: Human Health Perspectives and Future Directives.","volume":"8","author":"Pot\u00e9 Sivalingam","year":"2019","journal-title":"Pathogens]"},{"issue":"1","key":"pone.0310026.ref022","doi-asserted-by":"crossref","first-page":"3938","DOI":"10.1038\/s41598-019-40420-0","article-title":"Multi-state study of Enterobacteriaceae harboring extended-spectrum beta-lactamase and carbapenemase genes in U.S. drinking water.","volume":"9","author":"WD Tanner","year":"2019","journal-title":"Sci Rep"},{"issue":"4","key":"pone.0310026.ref023","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ijantimicag.2006.01.004","article-title":"Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp","volume":"27","author":"N Woodford","year":"2006","journal-title":"International Journal of Antimicrobial Agents"},{"key":"pone.0310026.ref024","doi-asserted-by":"crossref","first-page":"145925","DOI":"10.1016\/j.scitotenv.2021.145925","article-title":"Nationwide surveillance reveals frequent detection of carbapenemase-producing Enterobacterales in Dutch municipal wastewater","volume":"776","author":"H Blaak","year":"2021","journal-title":"Science of The Total Environment"},{"key":"pone.0310026.ref025","doi-asserted-by":"crossref","first-page":"122455","DOI":"10.1016\/j.envpol.2023.122455","article-title":"Transmission of clones of carbapenem-resistant Escherichia coli between a hospital and an urban wastewater treatment plant","volume":"336","author":"D Wang","year":"2023","journal-title":"Environmental Pollution"},{"issue":"2","key":"pone.0310026.ref026","doi-asserted-by":"crossref","first-page":"e0281909","DOI":"10.1371\/journal.pone.0281909","article-title":"Frequency and diversity of carbapenemase-producing Enterobacterales recovered from untreated wastewater impacted by selective media containing cefotaxime and meropenem in Ohio, USA.","volume":"18","author":"RJ Too","year":"2023","journal-title":"PLoS ONE"},{"issue":"23","key":"pone.0310026.ref027","doi-asserted-by":"crossref","first-page":"13906","DOI":"10.1021\/acs.est.7b03380","article-title":"Hospital Wastewater Releases of Carbapenem-Resistance Pathogens and Genes in Urban India","volume":"51","author":"M Lamba","year":"2017","journal-title":"Environ Sci Technol"},{"key":"pone.0310026.ref028","doi-asserted-by":"crossref","first-page":"156232","DOI":"10.1016\/j.scitotenv.2022.156232","article-title":"Distribution and characteristics of carbapenem-resistant and extended-spectrum \u03b2-lactamase (ESBL) producing Escherichia coli in hospital effluents, sewage treatment plants, and river water in an urban area of Japan.","volume":"839","author":"T Azuma","year":"2022","journal-title":"Science of The Total Environment"},{"issue":"5","key":"pone.0310026.ref029","doi-asserted-by":"crossref","first-page":"e1001644","DOI":"10.1371\/journal.pmed.1001644","article-title":"Fecal Contamination of Drinking-Water in Low- and Middle-Income Countries: A Systematic Review and Meta-Analysis.","volume":"11","author":"R Bain","year":"2014","journal-title":"PLoS Med"},{"issue":"4","key":"pone.0310026.ref030","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3390\/antibiotics13040295","article-title":"The Importance of Carbapenemase-Producing Enterobacterales in African Countries: Evolution and Current Burden.","volume":"13","author":"EC Chelaru","year":"2024","journal-title":"Antibiotics"},{"issue":"12","key":"pone.0310026.ref031","doi-asserted-by":"crossref","first-page":"e1255","DOI":"10.1016\/j.ijid.2013.07.016","article-title":"First report of NDM-1-producing Acinetobacter baumannii in East Africa.","volume":"17","author":"G Revathi","year":"2013","journal-title":"International Journal of Infectious Diseases"},{"issue":"7","key":"pone.0310026.ref032","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.ijmm.2017.07.006","article-title":"Molecular epidemiology of Klebsiella pneumoniae invasive infections over a decade at Kilifi County Hospital in Kenya.","volume":"307","author":"SP Henson","year":"2017","journal-title":"International Journal of Medical Microbiology"},{"issue":"2","key":"pone.0310026.ref033","doi-asserted-by":"crossref","first-page":"e0246937","DOI":"10.1371\/journal.pone.0246937","article-title":"Detection of diverse carbapenem and multidrug resistance genes and high-risk strain types among carbapenem non-susceptible clinical isolates of target gram-negative bacteria in Kenya.","volume":"16","author":"L Musila","year":"2021","journal-title":"PLoS ONE"},{"issue":"3","key":"pone.0310026.ref034","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1080\/14634980301484","article-title":"Characterization of environmental gradients using physico-chemical measurements and diatom densities in Nairobi River, Kenya.","volume":"6","author":"GG Ndiritu","year":"2003","journal-title":"Aquatic Ecosystem Health and Management"},{"issue":"4","key":"pone.0310026.ref035","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.watres.2004.11.011","article-title":"Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis","volume":"39","author":"RA Haugland","year":"2005","journal-title":"Water Research"},{"key":"pone.0310026.ref036","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.watres.2019.03.034","article-title":"Evaluation of rapid qPCR method for quantification of E. coli at non-point source impacted Lake Michigan beaches","volume":"156","author":"A Shrestha","year":"2019","journal-title":"Water Research"},{"issue":"9","key":"pone.0310026.ref037","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.3201\/eid1809.120355","article-title":"Rapid Detection of Carbapenemase-producing Enterobacteriaceae","volume":"18","author":"P Nordmann","year":"2012","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"pone.0310026.ref038","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.ijantimicag.2015.08.011","article-title":"A resurgence of \u03b2-lactamase inhibitor combinations effective against multidrug-resistant Gram-negative pathogens","volume":"46","author":"K. Bush","year":"2015","journal-title":"International Journal of Antimicrobial Agents"},{"issue":"12","key":"pone.0310026.ref039","doi-asserted-by":"crossref","first-page":"7553","DOI":"10.1128\/AAC.02761-13","article-title":"Evaluation of the Carba NP Test for Carbapenemase Detection","volume":"58","author":"M \u00d6sterblad","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"issue":"12","key":"pone.0310026.ref040","doi-asserted-by":"crossref","first-page":"6437","DOI":"10.1128\/AAC.01395-12","article-title":"Rapid Identification of Carbapenemase Types in Enterobacteriaceae and Pseudomonas spp. by Using a Biochemical Test","volume":"56","author":"L Dortet","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"issue":"12","key":"pone.0310026.ref041","doi-asserted-by":"crossref","first-page":"3908","DOI":"10.1128\/JCM.02032-15","article-title":"Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures.","volume":"53","author":"F Pasteran","year":"2015","journal-title":"J Clin Microbiol"},{"issue":"2","key":"pone.0310026.ref042","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.clinmicnews.2019.01.001","article-title":"Phenotypic Methods for Detection of Carbapenemase Production in Carbapenem-Resistant Organisms: What Method Should Your Laboratory Choose?","volume":"41","author":"M Workneh","year":"2019","journal-title":"Clinical Microbiology Newsletter"},{"issue":"4","key":"pone.0310026.ref043","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1128\/AAC.45.4.1151-1161.2001","article-title":"Novel Carbapenem-Hydrolyzing \u03b2-Lactamase, KPC-1, from a Carbapenem-Resistant Strain of Klebsiella pneumoniae","volume":"45","author":"H Yigit","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"pone.0310026.ref044","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1099\/jmm.0.005850-0","article-title":"Emergence of carbapenem-non-susceptible extended-spectrum \u03b2-lactamase-producing Klebsiella pneumoniae isolates at the university hospital of T\u00fcbingen, Germany.","volume":"58","author":"S Gr\u00f6bner","year":"2009","journal-title":"Journal of Medical Microbiology"},{"issue":"2","key":"pone.0310026.ref045","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.diagmicrobio.2011.06.013","article-title":"The characteristics of NDM-producing Klebsiella pneumoniae from Canada","volume":"71","author":"G Peirano","year":"2011","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"issue":"6","key":"pone.0310026.ref046","first-page":"372","article-title":"Maintenance of Carbapenemase-Producing Enterobacteriaceae in a Farrow-to-Finish Swine Production System. Foodborne Pathogens and Disease","volume":"15","author":"DF Mollenkopf","year":"2018","journal-title":"Internet]"},{"issue":"6","key":"pone.0310026.ref047","first-page":"e02209","article-title":"In Vitro Activity of Imipenem-Relebactam against Gram-Negative ESKAPE Pathogens Isolated by Clinical Laboratories in the United States in 2015 (Results from the SMART Global Surveillance Program).","volume":"61","author":"SH Lob","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"pone.0310026.ref048","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1128\/JCM.02368-08","article-title":"Evaluation of PCR-Based Testing for Surveillance of KPC-Producing Carbapenem-Resistant Members of the Enterobacteriaceae Family","volume":"47","author":"V Schechner","year":"2009","journal-title":"J Clin Microbiol"},{"issue":"2","key":"pone.0310026.ref049","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/jac\/dkl481","article-title":"Multiplex PCR for rapid detection of genes encoding acquired metallo- -lactamases","volume":"59","author":"MJ Ellington","year":"2006","journal-title":"Journal of Antimicrobial Chemotherapy"},{"issue":"5","key":"pone.0310026.ref050","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3343\/alm.2012.32.5.359","article-title":"Multiplex PCR for rapid detection of genes encoding class A carbapenemases.","volume":"32","author":"SS Hong","year":"2012","journal-title":"Ann Lab Med"},{"issue":"3","key":"pone.0310026.ref051","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1093\/jac\/dkp498","article-title":"Development of a set of multiplex PCR assays for the detection of genes encoding important \u03b2-lactamases in Enterobacteriaceae","volume":"65","author":"C Dallenne","year":"2010","journal-title":"Journal of Antimicrobial Chemotherapy"},{"issue":"1","key":"pone.0310026.ref052","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1093\/jac\/dkv283","article-title":"Detection of carbapenemase activity in Enterobacteriaceae: comparison of the carbapenem inactivation method versus the Carba NP test: Table 1","volume":"71","author":"N Tijet","year":"2016","journal-title":"J Antimicrob Chemother"},{"issue":"5","key":"pone.0310026.ref053","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1089\/mdr.2015.0072","article-title":"Low Prevalence of Carbapenem-Resistant Bacteria in River Water: Resistance Is Mostly Related to Intrinsic Mechanisms","volume":"21","author":"M Tac\u00e3o","year":"2015","journal-title":"Microbial Drug Resistance"},{"issue":"7","key":"pone.0310026.ref054","first-page":"425","article-title":"Detection of Carbapenem-Resistance Genes in Klebsiella Species Recovered from Selected Environmental Niches in the Eastern Cape Province, South Africa. Antibiotics","volume":"9","author":"KE Ebomah","year":"2020","journal-title":"Internet]"},{"issue":"9","key":"pone.0310026.ref055","doi-asserted-by":"crossref","first-page":"e00230","DOI":"10.1128\/jb.00230-22","article-title":"How Escherichia coli Became the Flagship Bacterium of Molecular Biology. O\u2019Toole G, editor","volume":"204","author":"N Ruiz","year":"2022","journal-title":"J Bacteriol"},{"issue":"9","key":"pone.0310026.ref056","doi-asserted-by":"crossref","first-page":"e01021","DOI":"10.1128\/AAC.01021-18","article-title":"Screening and Characterization of Multidrug-Resistant Gram-Negative Bacteria from a Remote African Area, S\u00e3o Tom\u00e9 and Pr\u00edncipe","volume":"62","author":"L Poirel","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"pone.0310026.ref057","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s12866-017-1012-8","article-title":"Structure, Genetics and Worldwide Spread of New Delhi Metallo-\u03b2-lactamase (NDM): a threat to public health.","volume":"17","author":"AU Khan","year":"2017","journal-title":"BMC Microbiol"},{"issue":"1","key":"pone.0310026.ref058","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","article-title":"Multiplex PCR for detection of acquired carbapenemase genes","volume":"70","author":"L Poirel","year":"2011","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"key":"pone.0310026.ref059","doi-asserted-by":"crossref","first-page":"892126","DOI":"10.3389\/fcimb.2022.892126","article-title":"Genomic transmission analysis of multidrug-resistant Gram-negative bacteria within a newborn unit of a Kenyan tertiary hospital: A four-month prospective colonization study.","volume":"12","author":"D Villinger","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"pone.0310026.ref060","first-page":"14","article-title":"Molecular surveillance reveals widespread colonisation by carbapenemase and extended spectrum beta-lactamase producing organisms in neonatal units in Kenya and Nigeria. Antimicrob Resist Infect Control","volume":"12","author":"T Edwards","year":"2023","journal-title":"Internet]"},{"issue":"6","key":"pone.0310026.ref061","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.nurpra.2019.02.013","article-title":"Surveillance Screening to Reduce Carbapenem Resistance.","volume":"15","author":"H Arnett","year":"2019","journal-title":"The Journal for Nurse Practitioners"},{"issue":"06","key":"pone.0310026.ref062","first-page":"422","article-title":"Dissemination of ST101 blaOXA-48 producing Klebsiella pneumoniae at tertiary care setting. 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