{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:10:59Z","timestamp":1793329859427,"version":"4.5.0"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Mushrooms are considered one of the safe and effective medications because they have great economic importance due to countless biological properties. <jats:italic>Cordyceps militaris<\/jats:italic> contains bioactive compounds with antioxidant, antimicrobial and anti-cancerous properties. This study was projected to analyze the potentials of biometabolites and to extract antimicrobial peptides and protein from the <jats:italic>C<\/jats:italic>. <jats:italic>militaris<\/jats:italic>. An <jats:italic>in-vitro<\/jats:italic> analysis of biometabolites and antimicrobial peptides was performed to investigate their pharmacological potentials followed by quantification and characterization of extracted protein. Computational analysis on non-small cell lung cancer genes (NSCLC) was performed on quantified compounds to interpret the biometabolites from <jats:italic>C<\/jats:italic>. <jats:italic>militaris<\/jats:italic> that could be potential drug candidate molecules with high specificity and potency. A total of 34 compounds representing 100% of total detected constituents identified were identified using GCMS analysis and 20 compounds using LC-MS which showed strong biological activities. FT-IR spectroscopy manifest powerful instant peaks to have different bioactive components including carboxylic acid, phenols, amines and alkanes present in methanolic extract of <jats:italic>C<\/jats:italic>. <jats:italic>militaris<\/jats:italic>. In <jats:italic>C<\/jats:italic>. <jats:italic>militaris<\/jats:italic>, higher protein concentration was observed in 70% concentration of protein extract (500 \u03bcg\/ml \u00b1 0.025). The best antioxidant activity (% Radical scavenging activity) of methanolic extracts was 80a \u00b1 0.03, antidiabetic activity was 37 \u00b1 0.057 and anti-inflammatory activity was 40 \u00b1 0.021 at 12 mg\/ml. Antibacterial activity for different concentrations of Cordyceps protein and methanolic extracts was significantly (p &lt; 0.05). Indolizine, 2-(4-methylphenyl) has most binding affinity (micromolar) and optimized properties to be selected as the lead inhibitor. It interacts favorably with the active site of RET gene of NSCLC and is neuroprotective and hepatoprotective.<\/jats:p>","DOI":"10.1371\/journal.pone.0310103","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T18:34:36Z","timestamp":1737657276000},"page":"e0310103","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Investigation of biometabolites and novel antimicrobial peptides derived from promising source Cordyceps militaris and effect of non-small cell lung cancer genes computationally"],"prefix":"10.1371","volume":"20","author":[{"given":"Muhammad","family":"Afzal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai Abdel Haleem A.","family":"Abusalah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelum","family":"Shehzadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Absar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1504-1705","authenticated-orcid":true,"given":"Naveed","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarmir","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalnaz","family":"Naseem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noshaba","family":"Mehmood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirnpal Kaur Banga","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,1,23]]},"reference":[{"issue":"1","key":"pone.0310103.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11274-017-2385-4","article-title":"Comparative nutritional and mycochemical contents, biological activities and LC\/MS screening of tuber from new recipe cultivation technique with wild type tuber of tiger\u2019s milk mushroom of species Lignosus rhinocerus","volume":"34","author":"NAM Jamil","year":"2018","journal-title":"World Journal of Microbiology and Biotechnology"},{"issue":"1","key":"pone.0310103.ref002","doi-asserted-by":"crossref","first-page":"43","DOI":"10.21608\/eajbsg.2023.285126","article-title":"Silver Nanoparticles Synthesized from Polyporus plorans, A wild Mushroom with Detecting Its Physio-Chemical Characterization and Antimicrobial Activities","volume":"15","author":"SM Marie","year":"2023","journal-title":"Egyptian Academic Journal of Biological Sciences, G Microbiology."},{"issue":"7","key":"pone.0310103.ref003","doi-asserted-by":"crossref","first-page":"503","DOI":"10.3390\/jof7070503","article-title":"Mycopharmaceuticals and nutraceuticals: promising agents to improve human well-being and life quality","volume":"7","author":"JR Al-Obaidi","year":"2021","journal-title":"Journal of Fungi"},{"issue":"5","key":"pone.0310103.ref004","first-page":"178","article-title":"Medicinal mushroom of potential pharmaceutical toxic importance: contribution in phytotherapy.","volume":"21","author":"ET Fokunang","year":"2022","journal-title":"Functional Food"},{"key":"pone.0310103.ref005","doi-asserted-by":"crossref","first-page":"102230","DOI":"10.1016\/j.jddst.2020.102230","article-title":"An in vitro cytotoxicity of a novel pH-Sensitive lectin loaded-cockle shell-derived calcium carbonate nanoparticles against MCF-7 breast tumour cell","volume":"61","author":"RI Mahmood","year":"2021","journal-title":"Journal of Drug Delivery Science and Technology"},{"issue":"4","key":"pone.0310103.ref006","first-page":"59","article-title":"Cordyceps: a highly coveted medicinal mushroom.","volume":"36","author":"J. 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