{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:22:43Z","timestamp":1787242963185,"version":"build-2736575974"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["PAPIIT IN100322"],"award-info":[{"award-number":["PAPIIT IN100322"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005739","name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["3\/2023"],"award-info":[{"award-number":["3\/2023"]}],"id":[{"id":"10.13039\/501100005739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Aggregation pathway of amyloid-\n                    <jats:italic>\u03b2<\/jats:italic>\n                    (25-35) in water affects the oxidative stress in the brain observed after administration of aggregated peptide in animals\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Our studies on peptide aggregation\n                    <jats:italic>ex situ<\/jats:italic>\n                    prior to injection suggest that from the onset of peptide incubation in aqueous media, all samples exhibit the formation of fibril-like aggregates, characterized by a significant amount of\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -sheets. This induces significant oxidative stress\n                    <jats:italic>in vivo<\/jats:italic>\n                    as observed for up to 60 min of peptide aggregation time. As the aggregation advances, the fibril-like aggregates become longer and intertwined, while the amount of\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -sheets does not change significantly. An injection of such large, thick, and entangled aggregates in the animal brain results in a drastic increase in oxidative stress. This may be related to the number of activated microglia that initiate a sequence of inflammatory responses in the presence of large, highly interconnected fibrils.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0310258","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T13:27:54Z","timestamp":1730208474000},"page":"e0310258","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of the aggregation state of amyloid-beta (25-35) on the brain oxidative stress in vivo"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4287-2953","authenticated-orcid":true,"given":"Anna","family":"Kozina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goethe","family":"Herbert-Alonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"D\u00edaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Guevara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,10,29]]},"reference":[{"key":"pone.0310258.ref001","first-page":"31","article-title":"A\u03b2 plaques","volume":"1","author":"L.C Walker","year":"2020","journal-title":"Free Neuropathology"},{"key":"pone.0310258.ref002","doi-asserted-by":"crossref","first-page":"a006270","DOI":"10.1101\/cshperspect.a006270","article-title":"Trafficking and proteolytic processing of APP","volume":"2","author":"C. 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