{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:25:14Z","timestamp":1790767514658,"version":"4.1.0"},"reference-count":93,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund (ERDF) PO","award":["20202640-0022937"],"award-info":[{"award-number":["20202640-0022937"]}]},{"DOI":"10.13039\/501100010081","name":"Conseil R\u00e9gional de La R\u00e9union","doi-asserted-by":"publisher","award":["DIRED\/20181182"],"award-info":[{"award-number":["DIRED\/20181182"]}],"id":[{"id":"10.13039\/501100010081","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010081","name":"Conseil R\u00e9gional de La R\u00e9union","doi-asserted-by":"publisher","award":["DIRED\/20181182"],"award-info":[{"award-number":["DIRED\/20181182"]}],"id":[{"id":"10.13039\/501100010081","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Dengue virus (DENV) is the most prevalent mosquito-borne <jats:italic>Flavivirus<\/jats:italic> that affects humans worldwide. <jats:italic>Aedes albopictus<\/jats:italic>, which is naturally infected with the bacteria <jats:italic>Wolbachia<\/jats:italic>, is considered to be a secondary vector of DENV. However, it was responsible for a recent DENV outbreak of unprecedented magnitude in Reunion Island, a French island in the South West Indian Ocean. Moreover, the distribution of the cases during this epidemic showed a spatially heterogeneous pattern across the island, leading to questions about the differential vector competence of mosquito populations from different geographic areas. The aim of this study was to gain a better understanding of the vector competence of the <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> populations from Reunion Island for local DENV epidemic strains, while considering their infection by <jats:italic>Wolbachia<\/jats:italic>. Experimental infections were conducted using ten populations of <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> sampled across Reunion Island and exposed to three DENV strains: one strain of DENV serotype 1 (DENV-1) and two strains of DENV serotype 2 (DENV-2). We analyzed three vector competence parameters including infection rate, dissemination efficiency and transmission efficiency, at different days post-exposition (dpe). We also assessed whether there was a correlation between the density of <jats:italic>Wolbachia<\/jats:italic> and viral load\/vector competence parameters. Our results show that the <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> populations tested were not able to transmit the two DENV-2 strains, while transmission efficiencies up to 40.79% were observed for the DENV-1 strain, probably due to difference in viral titres. Statistical analyses showed that the parameters mosquito population, generation, dpe and area of sampling significantly affect the transmission efficiencies of DENV-1. Although the density of <jats:italic>Wolbachia<\/jats:italic> varied according to mosquito population, no significant correlation was found between <jats:italic>Wolbachia<\/jats:italic> density and either viral load or vector competence parameters for DENV-1. Our results highlight the importance of using natural mosquito populations for a better understanding of transmission patterns of dengue.<\/jats:p>","DOI":"10.1371\/journal.pone.0310635","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T17:39:36Z","timestamp":1726767576000},"page":"e0310635","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vector competence of Aedes albopictus field populations from Reunion Island exposed to local epidemic dengue viruses"],"prefix":"10.1371","volume":"19","author":[{"given":"Sarah","family":"Hafsia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Barbar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoues","family":"Alout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Baudino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrille","family":"Lebon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Gomard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7806-4205","authenticated-orcid":true,"given":"Toscane","family":"Fouri\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Mavingui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0233-2239","authenticated-orcid":true,"given":"C\u00e9lestine","family":"Atyame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,9,19]]},"reference":[{"issue":"8","key":"pone.0310635.ref001","doi-asserted-by":"crossref","first-page":"e1760","DOI":"10.1371\/journal.pntd.0001760","article-title":"Refining the global spatial limits of Dengue virus transmission by evidence-based consensus.","volume":"6","author":"OJ Brady","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"issue":"9","key":"pone.0310635.ref002","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1038\/s41564-019-0476-8","article-title":"The current and future global distribution and population at risk of Dengue.","volume":"4","author":"JP Messina","year":"2019","journal-title":"Nat Microbiol"},{"issue":"7446","key":"pone.0310635.ref003","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of Dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"key":"pone.0310635.ref004","unstructured":"Centers for Disease Control and Prevention. About Dengue [Internet]. 2023 [cited 2023 Oct 2]. Available from: https:\/\/www.cdc.gov\/dengue\/about\/index.html"},{"key":"pone.0310635.ref005","unstructured":"World Health Organization. Dengue and severe dengue [Internet]. 2023 [cited 2023 Oct 2]. Available from: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/dengue-and-severe-dengue"},{"issue":"9","key":"pone.0310635.ref006","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.3390\/v3091562","article-title":"Dengue\u2014Quo tu et quo vadis?","volume":"3","author":"R Chen","year":"2011","journal-title":"Viruses"},{"key":"pone.0310635.ref007","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-3527(08)00401-6","article-title":"The history and evolution of human Dengue emergence","volume":"72","author":"N Vasilakis","year":"2008","journal-title":"Adv Virus Res"},{"issue":"4","key":"pone.0310635.ref008","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.meegid.2009.02.003","article-title":"Molecular evolution of Dengue viruses: contributions of phylogenetics to understanding the history and epidemiology of the preeminent arboviral disease","volume":"9","author":"SC Weaver","year":"2009","journal-title":"Infect Genet Evol"},{"issue":"1","key":"pone.0310635.ref009","doi-asserted-by":"crossref","first-page":"30","DOI":"10.4269\/ajtmh.1952.1.30","article-title":"Research on Dengue during World War II","volume":"1","author":"AB Sabin","year":"1952","journal-title":"Am J Trop Med Hyg"},{"issue":"7","key":"pone.0310635.ref010","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1007\/s00705-013-1645-3","article-title":"Secondary infection as a risk factor for Dengue hemorrhagic fever\/Dengue shock syndrome: an historical perspective and role of antibody-dependent enhancement of infection","volume":"158","author":"MG Guzman","year":"2013","journal-title":"Arch Virol"},{"issue":"6365","key":"pone.0310635.ref011","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1126\/science.aan6836","article-title":"Antibody-dependent enhancement of severe Dengue disease in humans","volume":"358","author":"LC Katzelnick","year":"2017","journal-title":"Science"},{"issue":"5","key":"pone.0310635.ref012","doi-asserted-by":"crossref","first-page":"e646","DOI":"10.1371\/journal.pntd.0000646","article-title":"Consequences of the expanding global distribution of Aedes albopictus for Dengue virus transmission.","volume":"4","author":"L Lambrechts","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"issue":"14\u201315","key":"pone.0310635.ref013","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/j.micinf.2009.05.005","article-title":"Aedes albopictus, an arbovirus vector: from the darkness to the light","volume":"11","author":"C Paupy","year":"2009","journal-title":"Microbes Infect"},{"issue":"1","key":"pone.0310635.ref014","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/1471-2458-12-72","article-title":"Aedes albopictus and the reemergence of Dengue.","volume":"12","author":"G. Rezza","year":"2012","journal-title":"BMC Public Health"},{"issue":"4","key":"pone.0310635.ref015","doi-asserted-by":"crossref","first-page":"267","DOI":"10.7601\/mez.49.267","article-title":"Dengue vector mosquitoes in Japan: the role of Aedes albopictus and Aedes aegypti in the 1942\u20131944 Dengue epidemics of Japanese main islands.","volume":"49","author":"S. Hotta","journal-title":"Med Entomol Zool. 1999"},{"issue":"5","key":"pone.0310635.ref016","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.4269\/ajtmh.17-0954","article-title":"Dengue virus infection in Aedes albopictus during the 2014 autochthonous dengue outbreak in Tokyo Metropolis, Japan","volume":"98","author":"D Kobayashi","year":"2018","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"pone.0310635.ref017","doi-asserted-by":"crossref","first-page":"742","DOI":"10.3201\/eid1105.041063","article-title":"Dengue fever, Hawaii, 2001\u20132002.","volume":"11","author":"PV Effler","year":"2005","journal-title":"Emerg Infect Dis"},{"issue":"7","key":"pone.0310635.ref018","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.3201\/eid1407.071521","article-title":"Outbreak of Dengue and Chikungunya fevers, Toamasina, Madagascar, 2006","volume":"14","author":"M Ratsitorahina","year":"2008","journal-title":"Emerg Infect Dis"},{"issue":"4","key":"pone.0310635.ref019","doi-asserted-by":"crossref","first-page":"591","DOI":"10.3201\/eid1504.080664","article-title":"Concurrent Chikungunya and Dengue virus infections during simultaneous outbreaks, Gabon, 2007","volume":"15","author":"EM Leroy","year":"2009","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"pone.0310635.ref020","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/1471-2458-12-83","article-title":"A local outbreak of Dengue caused by an imported case in Dongguan China.","volume":"12","author":"HJ Peng","year":"2012","journal-title":"BMC Public Health"},{"issue":"6","key":"pone.0310635.ref021","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.4269\/ajtmh.2007.76.1182","article-title":"An outbreak of Dengue virus serotype 1 infection in Cixi, Ningbo, People\u2019s Republic of China, 2004, associated with a traveler from Thailand and high density of Aedes albopictus","volume":"76","author":"G Xu","year":"2007","journal-title":"Am J Trop Med Hyg"},{"issue":"7","key":"pone.0310635.ref022","doi-asserted-by":"crossref","first-page":"e0010547","DOI":"10.1371\/journal.pntd.0010547","article-title":"Overview of Dengue outbreaks in the southwestern Indian Ocean and analysis of factors involved in the shift toward endemicity in Reunion Island: a systematic review.","volume":"16","author":"S Hafsia","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"pone.0310635.ref023","first-page":"205","article-title":"Dengue on R\u00e9union. Isolation of a strain at the Pasteur Institute of Madagascar.","volume":"72","author":"P Coulanges","year":"1979","journal-title":"Bull Soc Pathol Exot Filiales."},{"issue":"2","key":"pone.0310635.ref024","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s13149-013-0288-7","article-title":"Inventaire actualis\u00e9 des moustiques (Diptera: Culicidae) de l\u2019\u00eele de La R\u00e9union, Oc\u00e9an Indien.","volume":"106","author":"P Bouss\u00e8s","year":"2013","journal-title":"Bull Soci\u00e9t\u00e9 Pathol Exot"},{"issue":"2","key":"pone.0310635.ref025","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1080\/00379271.2018.1429951","article-title":"Inventaire des moustiques (Diptera: Culicidae) des \u00eeles du sud-ouest de l\u2019oc\u00e9an Indien, Madagascar except\u00e9\u2014Une revue critique.","volume":"54","author":"P Bouss\u00e8s","year":"2018","journal-title":"Ann Soci\u00e9t\u00e9 Entomol Fr NS"},{"key":"pone.0310635.ref026","first-page":"323","article-title":"Aedes albopictus et Aedes aegypti \u00e0 l\u2019\u00eele de La R\u00e9union.","volume":"74","author":"M Salvan","year":"1994","journal-title":"Ann Soc Belg Med Trop"},{"issue":"3","key":"pone.0310635.ref027","doi-asserted-by":"crossref","first-page":"e2111","DOI":"10.1371\/journal.pntd.0002111","article-title":"Evidence of habitat structuring Aedes albopictus populations in R\u00e9union Island.","volume":"7","author":"H Delatte","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"pone.0310635.ref028","doi-asserted-by":"crossref","first-page":"727","DOI":"10.4269\/ajtmh.2007.77.727","article-title":"A major epidemic of Chikungunya virus infection on R\u00e9union Island, France, 2005\u20132006.","volume":"77","author":"P Renault","year":"2007","journal-title":"Am J Trop Med Hyg"},{"issue":"9","key":"pone.0310635.ref029","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.medmal.2010.11.021","article-title":"Dengue \u00e0 La R\u00e9union et dans les \u00eeles du sud-ouest de l\u2019oc\u00e9an Indien.","volume":"41","author":"E D\u2019Ortenzio","year":"2011","journal-title":"M\u00e9decine Mal Infect."},{"key":"pone.0310635.ref030","article-title":"Epid\u00e9mie de Dengue 1 \u00e0 La R\u00e9union en","author":"V Pierre","year":"2004"},{"key":"pone.0310635.ref031","unstructured":"Sant\u00e9 Publique France. Points Epid\u00e9miologiques\u2014Dengue\u2014Oc\u00e9an Indien [Internet]. 2021 [cited 2021 Sep 8]. Available from: https:\/\/www.santepubliquefrance.fr\/recherche\/#search=dengue&themes=dengue&regions=Oc%C3%A9an%20Indien"},{"key":"pone.0310635.ref032","doi-asserted-by":"crossref","first-page":"104687","DOI":"10.1016\/j.rinp.2021.104687","article-title":"Dynamic mapping of Dengue basic reproduction number","volume":"29","author":"S Benkimoun","year":"2021","journal-title":"Results Phys"},{"issue":"1","key":"pone.0310635.ref033","doi-asserted-by":"crossref","first-page":"e0227407","DOI":"10.1371\/journal.pone.0227407","article-title":"Complementarity of empirical and process-based approaches to modelling mosquito population dynamics with Aedes albopictus as an example\u2014Application to the development of an operational mapping tool of vector populations","volume":"15","author":"A Tran","year":"2020","journal-title":"PLOS ONE"},{"issue":"6","key":"pone.0310635.ref034","doi-asserted-by":"crossref","first-page":"e706","DOI":"10.1371\/journal.pntd.0000706","article-title":"Orally co-infected Aedes albopictus from La Reunion Island, Indian Ocean, can deliver both Dengue and Chikungunya infectious viral particles in their saliva.","volume":"4","author":"M Vazeille","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"issue":"12","key":"pone.0310635.ref035","doi-asserted-by":"crossref","first-page":"e1989","DOI":"10.1371\/journal.pntd.0001989","article-title":"The native Wolbachia symbionts limit transmission of Dengue virus in Aedes albopictus.","volume":"6","author":"L Mousson","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0310635.ref036","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1046\/j.1365-2540.2001.00866.x","article-title":"Population structure of Aedes albopictus from La Reunion Island (Indian Ocean) with respect to susceptibility to a Dengue virus.","volume":"87","author":"C Paupy","year":"2001","journal-title":"Heredity"},{"issue":"1","key":"pone.0310635.ref037","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1186\/s13071-019-3840-x","article-title":"High genetic diversity but no geographical structure of Aedes albopictus populations in R\u00e9union Island.","volume":"12","author":"AC Latreille","year":"2019","journal-title":"Parasit Vectors"},{"key":"pone.0310635.ref038","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.meegid.2017.12.018","article-title":"Genetic diversity and distribution differ between long-established and recently introduced populations in the invasive mosquito Aedes albopictus","volume":"58","author":"S Sherpa","year":"2018","journal-title":"Infect Genet Evol"},{"issue":"1360","key":"pone.0310635.ref039","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1098\/rspb.1995.0117","article-title":"Evolution and phylogeny of Wolbachia: reproductive parasites of arthropods","volume":"261","author":"JH Werren","year":"1995","journal-title":"Proc R Soc Lond B Biol Sci"},{"issue":"1","key":"pone.0310635.ref040","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1186\/s13071-017-2493-x","article-title":"The impact of temperature and Wolbachia infection on vector competence of potential Dengue vectors Aedes aegypti and Aedes albopictus in the transmission of Dengue virus serotype 1 in southern Taiwan.","volume":"10","author":"CH Tsai","year":"2017","journal-title":"Parasit Vectors"},{"key":"pone.0310635.ref041","doi-asserted-by":"crossref","first-page":"649975","DOI":"10.3389\/fcimb.2021.649975","article-title":"Vector competence for DENV-2 among Aedes albopictus (Diptera: Culicidae) populations in China.","volume":"11","author":"Y Wei","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0310635.ref042","unstructured":"NASA. Snow Peak, R\u00e9union Island; 2023 [cited 2024 Jun 2]. Database: NASA\u2019s Earth Observatory [Internet]. Available from: https:\/\/earthobservatory.nasa.gov\/images\/152265\/snow-peak-reunion-island"},{"issue":"1","key":"pone.0310635.ref043","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1186\/s12879-023-08125-y","article-title":"Genetic characterization of Dengue virus serotype 1 circulating in Reunion Island, 2019\u20132021, and the Seychelles, 2015\u20132016.","volume":"23","author":"S Hafsia","year":"2023","journal-title":"BMC Infect Dis."},{"issue":"4","key":"pone.0310635.ref044","doi-asserted-by":"crossref","first-page":"e01443","DOI":"10.1128\/MRA.01443-19","article-title":"Complete genome sequences of Dengue virus type 2 epidemic strains from Reunion Island and the Seychelles.","volume":"9","author":"H Pascalis","year":"2020","journal-title":"Microbiol Resour Announc."},{"issue":"6","key":"pone.0310635.ref045","doi-asserted-by":"crossref","first-page":"e5895","DOI":"10.1371\/journal.pone.0005895","article-title":"Chikungunya virus and Aedes mosquitoes: saliva is infectious as soon as two days after oral infection.","volume":"4","author":"M Dubrulle","year":"2009","journal-title":"PLOS ONE"},{"issue":"2","key":"pone.0310635.ref046","first-page":"65","article-title":"A simple sequentially rejective multiple test procedure.","volume":"6","author":"S. Holm","year":"1979","journal-title":"Scand J Stat"},{"key":"pone.0310635.ref047","volume-title":"An R Companion to Applied Regression [Internet].","author":"J Fox","year":"2019","edition":"3"},{"key":"pone.0310635.ref048","article-title":"Emmeans: Estimated Marginal Means, aka Least-Squares Means.","author":"V. Russell","year":"2021"},{"key":"pone.0310635.ref049","author":"R. Scherer","year":"2013","journal-title":"PropCIs: Various Confidence Interval Methods for Proportions [Internet]."},{"key":"pone.0310635.ref050","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: Elegant Graphics for Data Analysis [Internet].","author":"H Wickham","year":"2016"},{"key":"pone.0310635.ref051","author":"A. Kassambara","year":"2023","journal-title":"rstatix: Pipe-Friendly Framework for Basic Statistical Tests [Internet]."},{"key":"pone.0310635.ref052","doi-asserted-by":"crossref","first-page":"774846","DOI":"10.3389\/fgene.2021.774846","article-title":"Use ggbreak to effectively utilize plotting space to deal with large datasets and outliers.","volume":"12","author":"S Xu","year":"2021","journal-title":"Front Genet"},{"key":"pone.0310635.ref053","doi-asserted-by":"crossref","first-page":"12","DOI":"10.32614\/RJ-2011-012","article-title":"glm2: Fitting generalized linear models with convergence problems.","volume":"2","author":"I. Marschner","year":"2011","journal-title":"R J."},{"issue":"3","key":"pone.0310635.ref054","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1093\/jmedent\/30.3.524","article-title":"Vector competence of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) to Dengue 1 virus on Taiwan: development of the virus in orally and parenterally infected mosquitoes.","volume":"30","author":"WJ Chen","year":"1993","journal-title":"J Med Entomol"},{"issue":"3","key":"pone.0310635.ref055","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.0269-283X.2004.00513.x","article-title":"Critical review of the vector status of Aedes albopictus","volume":"18","author":"NG Gratz","year":"2004","journal-title":"Med Vet Entomol"},{"issue":"12","key":"pone.0310635.ref056","doi-asserted-by":"crossref","first-page":"e0007985","DOI":"10.1371\/journal.pntd.0007985","article-title":"Risk of Dengue in Central Africa: vector competence studies with Aedes aegypti and Aedes albopictus (Diptera: Culicidae) populations and Dengue 2 virus.","volume":"13","author":"B Kamgang","year":"2019","journal-title":"PLoS Negl Trop Dis."},{"key":"pone.0310635.ref057","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.meegid.2018.11.009","article-title":"Aedes aegypti vector competence studies: A review","volume":"67","author":"JA Souza-Neto","year":"2019","journal-title":"Infect Genet Evol"},{"issue":"8","key":"pone.0310635.ref058","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1093\/infdis\/jiv173","article-title":"Comparative susceptibility of Aedes albopictus and Aedes aegypti to Dengue virus infection after feeding on blood of viremic humans: implications for public health","volume":"212","author":"J Whitehorn","year":"2015","journal-title":"J Infect Dis"},{"issue":"4","key":"pone.0310635.ref059","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1603\/ME11293","article-title":"Vector competence of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) for Dengue virus in the Florida Keys.","volume":"49","author":"SL Richards","year":"2012","journal-title":"J Med Entomol"},{"key":"pone.0310635.ref060","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.actatropica.2016.05.018","article-title":"Vector competence of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) for the DEN2-FJ10 and DEN2-FJ11 strains of the Dengue 2 virus in Fujian, China.","volume":"161","author":"XX Guo","year":"2016","journal-title":"Acta Trop"},{"issue":"4","key":"pone.0310635.ref061","doi-asserted-by":"crossref","first-page":"266","DOI":"10.3390\/pathogens9040266","article-title":"A systematic review: is Aedes albopictus an efficient bridge vector for zoonotic arboviruses?","volume":"9","author":"T Pereira-dos-Santos","year":"2020","journal-title":"Pathogens"},{"issue":"2","key":"pone.0310635.ref062","doi-asserted-by":"crossref","first-page":"233","DOI":"10.56808\/2985-1130.2827","article-title":"Effects of Chikungunya virus titers in blood meals on virus infection, dissemination, and transmission in Asian tiger mosquito: Aedes albopictus (Diptera: Culicidae).","volume":"47","author":"R Tuanudom","year":"2017","journal-title":"Thai J Vet Med"},{"issue":"9","key":"pone.0310635.ref063","doi-asserted-by":"crossref","first-page":"867","DOI":"10.3390\/v11090867","article-title":"Vector competence: what Has Zika virus taught us?","volume":"11","author":"SR Azar","year":"2019","journal-title":"Viruses"},{"issue":"2","key":"pone.0310635.ref064","doi-asserted-by":"crossref","first-page":"e0007116","DOI":"10.1371\/journal.pntd.0007116","article-title":"Feasibility of feeding Aedes aegypti mosquitoes on Dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru.","volume":"13","author":"KC Long","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"pone.0310635.ref065","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.actatropica.2013.08.006","article-title":"Vector competence of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) for DEN2-43 and New Guinea C virus strains of Dengue 2 virus.","volume":"128","author":"XX Guo","year":"2013","journal-title":"Acta Trop"},{"issue":"9","key":"pone.0310635.ref066","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1111\/tmi.12918","article-title":"Vector competence of Malaysian Aedes albopictus with and without Wolbachia to four Dengue virus serotypes","volume":"22","author":"S Joanne","year":"2017","journal-title":"Trop Med Int Health"},{"issue":"2","key":"pone.0310635.ref067","doi-asserted-by":"crossref","first-page":"140","DOI":"10.7883\/yoken.JJID.2021.376","article-title":"Aedes albopictus strain and Dengue virus serotype in the Dengue fever outbreaks in Japan: implications of Wolbachia Infection","volume":"75","author":"T Sasaki","year":"2022","journal-title":"Jpn J Infect Dis"},{"issue":"11","key":"pone.0310635.ref068","doi-asserted-by":"crossref","first-page":"e1168","DOI":"10.1371\/journal.pone.0001168","article-title":"Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.","volume":"2","author":"M Vazeille","year":"2007","journal-title":"PLOS ONE."},{"issue":"12","key":"pone.0310635.ref069","doi-asserted-by":"crossref","first-page":"e201","DOI":"10.1371\/journal.ppat.0030201","article-title":"A single mutation in Chikungunya virus affects vector specificity and epidemic potential.","volume":"3","author":"KA Tsetsarkin","year":"2007","journal-title":"PLOS Pathog"},{"key":"pone.0310635.ref070","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.bjid.2019.11.003","article-title":"Differential replicative fitness of the four Dengue virus serotypes circulating in Colombia in human liver Huh7 cells","volume":"24","author":"H Guti\u00e9rrez-Barbosa","year":"2020","journal-title":"Braz J Infect Dis"},{"key":"pone.0310635.ref071","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.coviro.2015.10.003","article-title":"Dissecting vectorial capacity for mosquito-borne viruses.","volume":"15","author":"LD Kramer","year":"2015","journal-title":"Curr Opin Virol"},{"issue":"10","key":"pone.0310635.ref072","doi-asserted-by":"crossref","first-page":"e08259","DOI":"10.1016\/j.heliyon.2021.e08259","article-title":"Diversity of midgut microbiota in laboratory-colonized and field-collected Aedes albopictus (Diptera: Culicidae): a preliminary study.","volume":"7","author":"R Tuanudom","year":"2021","journal-title":"Heliyon"},{"issue":"1","key":"pone.0310635.ref073","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/1471-2180-9-96","article-title":"Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector","volume":"9","author":"A Rani","year":"2009","journal-title":"BMC Microbiol"},{"key":"pone.0310635.ref074","first-page":"9","article-title":"Microbiome interaction networks and community structure from laboratory-reared and field-collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquito vectors.","volume":"10","author":"S Hegde","year":"2018","journal-title":"Front Microbiol."},{"key":"pone.0310635.ref075","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.3389\/fmicb.2019.02036","article-title":"Aedes spp.and their microbiota: a review.","volume":"10","author":"F Scolari","year":"2019","journal-title":"Front Microbiol."},{"issue":"4","key":"pone.0310635.ref076","first-page":"257","article-title":"Study of the effect of the midgut bacterial flora of Culex quinquefasciatus on the susceptibility of mosquitoes to Japanese encephalitis virus","volume":"46","author":"DT Mourya","year":"2002","journal-title":"Acta Virol"},{"issue":"5","key":"pone.0310635.ref077","doi-asserted-by":"crossref","first-page":"e1000423","DOI":"10.1371\/journal.ppat.1000423","article-title":"Implication of the mosquito midgut microbiota in the defense against malaria parasites.","volume":"5","author":"Y Dong","year":"2009","journal-title":"PLOS Pathog."},{"issue":"3","key":"pone.0310635.ref078","doi-asserted-by":"crossref","first-page":"e1561","DOI":"10.1371\/journal.pntd.0001561","article-title":"Reciprocal tripartite interactions between the Aedes aegypti midgut microbiota, innate immune system and Dengue virus influences vector competence.","volume":"6","author":"JL Ramirez","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"pone.0310635.ref079","doi-asserted-by":"crossref","first-page":"4294","DOI":"10.3390\/v6114294","article-title":"The insect microbiome modulates vector competence for arboviruses.","volume":"6","author":"N Jupatanakul","year":"2014","journal-title":"Viruses"},{"issue":"1","key":"pone.0310635.ref080","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s40168-018-0435-2","article-title":"The mosquito holobiont: fresh insight into mosquito-microbiota interactions.","volume":"6","author":"M Gu\u00e9gan","year":"2018","journal-title":"Microbiome"},{"issue":"2","key":"pone.0310635.ref081","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.pt.2019.12.001","article-title":"Mosquito microbiota and implications for disease control","volume":"36","author":"H Gao","year":"2020","journal-title":"Trends Parasitol"},{"issue":"2","key":"pone.0310635.ref082","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1603\/ME09268","article-title":"Interactions between La Crosse virus and bacteria isolated from the digestive tract of Aedes albopictus (Diptera: Culicidae).","volume":"48","author":"JD Joyce","year":"2011","journal-title":"J Med Entomol"},{"issue":"7","key":"pone.0310635.ref083","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.cell.2009.11.042","article-title":"A Wolbachia symbiont in Aedes aegypti limits infection with Dengue, Chikungunya, and Plasmodium","volume":"139","author":"LA Moreira","year":"2009","journal-title":"Cell"},{"issue":"10","key":"pone.0310635.ref084","doi-asserted-by":"crossref","first-page":"e0011674","DOI":"10.1371\/journal.pntd.0011674","article-title":"Wolbachia-mediated resistance to Zika virus infection in Aedes aegypti is dominated by diverse transcriptional regulation and weak evolutionary pressures.","volume":"17","author":"EC Boehm","year":"2023","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"pone.0310635.ref085","doi-asserted-by":"crossref","first-page":"5705","DOI":"10.3390\/v7112903","article-title":"The impact of Wolbachia on virus infection in mosquitoes.","volume":"7","author":"K. Johnson","year":"2015","journal-title":"Viruses"},{"issue":"3","key":"pone.0310635.ref086","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pt.2015.10.011","article-title":"Exploiting intimate relationships: controlling mosquito-transmitted disease with Wolbachia","volume":"32","author":"EP Caragata","year":"2016","journal-title":"Trends Parasitol"},{"issue":"1","key":"pone.0310635.ref087","doi-asserted-by":"crossref","first-page":"32","DOI":"10.3390\/vaccines9010032","article-title":"A review: Aedes-borne arboviral infections, controls and Wolbachia-based strategies.","volume":"9","author":"ST Ogunlade","year":"2021","journal-title":"Vaccines"},{"key":"pone.0310635.ref088","doi-asserted-by":"crossref","first-page":"626329","DOI":"10.3389\/fimmu.2020.626329","article-title":"The antiviral effects of the symbiont bacteria Wolbachia in insects.","volume":"11","author":"AC Pimentel","year":"2021","journal-title":"Front Immunol"},{"key":"pone.0310635.ref089","doi-asserted-by":"crossref","first-page":"690087","DOI":"10.3389\/fcimb.2021.690087","article-title":"Intracellular interactions between arboviruses and Wolbachia in Aedes aegypti","volume":"11","author":"JIL Reyes","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"pone.0310635.ref090","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3376\/1081-1710(2008)33[173:RWDOFA]2.0.CO;2","article-title":"Relative Wolbachia density of field-collected Aedes albopictus mosquitoes in Thailand.","volume":"33","author":"A Ahantarig","year":"2008","journal-title":"J Vector Ecol"},{"issue":"3","key":"pone.0310635.ref091","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1574-6941.2010.01012.x","article-title":"Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes aegypti, from different geographic regions of Madagascar","volume":"75","author":"K Zouache","year":"2011","journal-title":"FEMS Microbiol Ecol"},{"issue":"1","key":"pone.0310635.ref092","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s13071-020-3899-4","article-title":"Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China.","volume":"13","author":"Y Hu","year":"2020","journal-title":"Parasit Vectors"},{"issue":"3","key":"pone.0310635.ref093","doi-asserted-by":"crossref","first-page":"e9700","DOI":"10.1371\/journal.pone.0009700","article-title":"Wolbachia age-sex-specific density in Aedes albopictus: a host evolutionary response to cytoplasmic incompatibility?","volume":"5","author":"P Tortosa","year":"2010","journal-title":"PLOS ONE."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0310635","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T17:40:02Z","timestamp":1726767602000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0310635"}},"subtitle":[],"editor":[{"given":"Humberto","family":"Lanz-Mendoza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,9,19]]},"references-count":93,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0310635","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,19]]}}}