{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:47:28Z","timestamp":1788587248100,"version":"build-2803163510"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Phytochemicals derived from plants have gained significant attention in recent years due to their diverse therapeutic properties.\n                    <jats:italic>Typha elephantina<\/jats:italic>\n                    is an aquatic plant having ameliorative characteristics like antioxidant, anti-inflammatory and analgesic etc. This research aims to conduct a comprehensive phytochemical investigation by Tandem mass spectrometry on the aerial parts and roots of\n                    <jats:italic>Typha elephantina<\/jats:italic>\n                    with a focus on identifying and characterizing the bioactive compounds present in it. Maceration in methanol, preliminary, MS\/MS analyses and DPPH antioxidant assay were carried out on this plant. This study led to the elucidation of 62 chemical constituents for the first time in\n                    <jats:italic>Typha elephantina<\/jats:italic>\n                    . 36 phytochemical compounds from aerial parts and 26 from roots\n                    <jats:italic>i<\/jats:italic>\n                    .\n                    <jats:italic>e<\/jats:italic>\n                    .,\n                    <jats:italic>p<\/jats:italic>\n                    -coumaric acid, caffeic acid, dihydrocaffeic acid, ferulic acid derivative, dehydroascorbic acid derivative, 1-\n                    <jats:italic>O<\/jats:italic>\n                    -coumaroyl glycerol, glucaroyl-4-hydroxy benzoate, apigenin derivative, 3-\n                    <jats:italic>O<\/jats:italic>\n                    -glucopyranosyl isorhamnetin, isovitexin derivative, rutin, isorhamnetin diglycosides, verbascoside, forsythoside A, pinocembrin, dihydro quercetin, prunetin, ampelopsin, daidzein, genistein, catechin and procyanidin B1 were detected within this plant specimen. The DPPH assay results showed that aerial parts TE(1), TE(2) showed more antioxidant activity than roots TER\/MeOH. These might be responsible for the understanding of the therapeutic potential of\n                    <jats:italic>Typha elephantina<\/jats:italic>\n                    and provide a foundation for future pharmacological studies.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0311549","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T13:33:23Z","timestamp":1733405603000},"page":"e0311549","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unraveling the bioactive constituents of Typha elephantina: A comprehensive phytochemical analysis by tandem mass spectrometry"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5361-0064","authenticated-orcid":true,"given":"Bibi","family":"Rohida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Farman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7538-8844","authenticated-orcid":true,"given":"Alina","family":"George","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"pone.0311549.ref001","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.indcrop.2018.11.051","article-title":"Phytochemical profiles and screening of \u03b1-glucosidase inhibitors of four Acer species leaves with ultra-filtration combined with UPLC-QTOF-MS\/MS","volume":"129","author":"L Zhang","year":"2019","journal-title":"Industrial crops and products"},{"key":"pone.0311549.ref002","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.indcrop.2018.04.037","article-title":"Phytochemical analysis and biological evaluation of three selected Cordia species from Panama","volume":"120","author":"G Marini","year":"2018","journal-title":"Industrial crops and products"},{"key":"pone.0311549.ref003","doi-asserted-by":"crossref","first-page":"100794","DOI":"10.1016\/j.rechem.2023.100794","article-title":"HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (Lf) Druce plant and molecular docking approach","volume":"5","author":"NB Krishnamurthy","year":"2023","journal-title":"Results in Chemistry"},{"issue":"6","key":"pone.0311549.ref004","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1021\/np50072a005","article-title":"Stigmasterols from Typha latifolia","volume":"53","author":"M Della Greca","year":"1990","journal-title":"Journal of Natural Products"},{"issue":"3","key":"pone.0311549.ref005","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0031-9422(01)00449-6","article-title":"Essential fatty acids and phenolic acids from extracts and leachates of southern cattail (Typha domingensis P.)","volume":"59","author":"MT Gallardo-Williams","year":"2002","journal-title":"Phytochemistry"},{"issue":"12","key":"pone.0311549.ref006","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1007\/s12272-010-1208-z","article-title":"A potential inhibitor of rat aortic vascular smooth muscle cell proliferation from the pollen of Typha angustata","volume":"33","author":"NX Nhiem","year":"2010","journal-title":"Archives of pharmacal research"},{"issue":"5","key":"pone.0311549.ref007","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/pca.1302","article-title":"Simultaneous determination of eleven major flavonoids in the pollen of Typha angustifolia by HPLC-PDA-MS","volume":"22","author":"W Tao","year":"2011","journal-title":"Phytochemical analysis: PCA"},{"issue":"7","key":"pone.0311549.ref008","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1080\/14786419.2018.1539979","article-title":"Identification of anti-nociceptive constituents from the pollen of Typha angustifolia L. using effect-directed fractionation","volume":"34","author":"G Zeng","year":"2020","journal-title":"Natural product research"},{"key":"pone.0311549.ref009","doi-asserted-by":"crossref","first-page":"104460","DOI":"10.1016\/j.bse.2022.104460","article-title":"Chemical constituents of the pollen of Typha angustifolia l","volume":"104","author":"JH Ke","year":"2022","journal-title":"Biochemical Systematics and Ecology"},{"key":"pone.0311549.ref010","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.phytol.2015.05.010","article-title":"Gas chromatography mass spectrometry based profiling of alkyl coumarates and ferulates in two species of cattail (Typha domingensis P., and Typha latifolia L.)","volume":"13","author":"D He","year":"2015","journal-title":"Phytochemistry Letters"},{"issue":"3","key":"pone.0311549.ref011","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.fitote.2009.08.026","article-title":"Two new cerebrosides from the pollen of Typha angustifolia","volume":"81","author":"WW Tao","year":"2010","journal-title":"Fitoterapia"},{"issue":"6","key":"pone.0311549.ref012","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/0049-3848(90)90162-6","article-title":"The antiatherogenic effects of components isolated from pollen Typhae","volume":"57","author":"J Zhao","year":"1990","journal-title":"Thrombosis research"},{"issue":"1","key":"pone.0311549.ref013","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.jep.2011.05.042","article-title":"Typha latifolia L. fruit polysaccharides induce the differentiation and stimulate the proliferation of human keratinocytes in 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NE Br.(bulrush) extract scavenges free radicals, inhibits collagenase activity and affects human sperm motility and mitochondrial membrane potential in vitro: a pilot study","volume":"44","author":"R Henkel","year":"2012","journal-title":"Andrologia"},{"issue":"5","key":"pone.0311549.ref016","first-page":"81","article-title":"Evaluation of in vitro anti-thrombolytic activity and cytotoxicity potential of Typha angustifolia L leaves extracts","volume":"6","author":"MK Umesh","year":"2014","journal-title":"Int J Pharm Pharm Sci"},{"key":"pone.0311549.ref017","first-page":"57","article-title":"Constituents of Typha elephantina","volume":"13","author":"NIJSIRI Ruangrungsi","year":"1987","journal-title":"J Sci Society"},{"key":"pone.0311549.ref018","volume-title":"The useful plants of india: with notices of their chief value in commerce, medicine, and the arts","author":"H. Drury","year":"1873","edition":"2"},{"key":"pone.0311549.ref019","first-page":"617","volume-title":"Flora of egypt: monocotyledons (Alismataceae-Orchidaceae)","author":"L. Boulos","year":"2005"},{"issue":"11","key":"pone.0311549.ref020","first-page":"5546","article-title":"Typha elephantina Roxb.: a review on ethanomedicinal, morphological, phytochemical and pharmacological perspectives","volume":"13","author":"G Singh","year":"2020","journal-title":"Research Journal of Pharmacy and Technology"},{"issue":"4","key":"pone.0311549.ref021","first-page":"10468","article-title":"In silico Antidiabetic Characterization and ADME Studies of Nymphaea Alba and Typha elephantina Phytoconstituents","volume":"29","author":"S Agnihotri","year":"2020","journal-title":"Int. J. Adv. Sci"},{"issue":"4","key":"pone.0311549.ref022","doi-asserted-by":"crossref","first-page":"350","DOI":"10.26574\/maedica.2019.14.4.350","article-title":"Preliminary analysis of phytoconstituents and evaluation of anthelminthic property of Cayratia auriculata (in vitro)","volume":"14","author":"N Kancherla","year":"2019","journal-title":"Maedica"},{"issue":"11","key":"pone.0311549.ref023","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.3390\/antiox10111770","article-title":"LC-MS\/MS-QTOF screening and identification of phenolic compounds from australian grown herbs and their antioxidant potential.","volume":"10","author":"A Ali","year":"2021","journal-title":"Antioxidants"},{"issue":"12","key":"pone.0311549.ref024","doi-asserted-by":"crossref","first-page":"6219","DOI":"10.3390\/ijms22126219","article-title":"Flavonoid and phenolic acids content and in vitro study of the potential anti-aging properties of Eutrema japonicum (Miq.) Koidz cultivated in Wasabi Farm Poland","volume":"22","author":"K Szewczyk","year":"2021","journal-title":"International Journal of Molecular Sciences"},{"issue":"2","key":"pone.0311549.ref025","first-page":"402","article-title":"Structural study of phenolic acids by triple quadrupole mass spectrometry with electrospray ionization in negative mode and H\/D isotopic exchange","volume":"31","author":"N Sinosaki","year":"2020","journal-title":"Journal of the Brazilian Chemical Society"},{"issue":"8","key":"pone.0311549.ref026","doi-asserted-by":"crossref","first-page":"11760","DOI":"10.3390\/molecules190811760","article-title":"Comprehensive phenolic profiling of Cyclopia genistoides (L.) Vent. by LC-DAD-MS and-MS\/MS reveals novel xanthone and benzophenone constituents","volume":"19","author":"T Beelders","year":"2014","journal-title":"Molecules"},{"issue":"3","key":"pone.0311549.ref027","doi-asserted-by":"crossref","first-page":"512","DOI":"10.3390\/ijms18030512","article-title":"Tentative characterization of polyphenolic compounds in the male flowers of Phoenix dactylifera by liquid chromatography coupled with mass spectrometry and DFT","volume":"18","author":"R Ben Said","year":"2017","journal-title":"International journal of molecular sciences"},{"key":"pone.0311549.ref028","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.foodchem.2016.05.052","article-title":"Identification and characterization of phenolic compounds in hydromethanolic extracts of sorghum wholegrains by LC-ESI-MSn","volume":"211","author":"J Kang","year":"2016","journal-title":"Food chemistry"},{"issue":"12","key":"pone.0311549.ref029","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1039\/b007299k","article-title":"An electrospray ionization ion trap mass spectrometric (ESI-MS-MSn) study of dehydroascorbic acid hydrolysis at neutral pH","volume":"125","author":"N Cioffi","year":"2000","journal-title":"Analyst"},{"issue":"22","key":"pone.0311549.ref030","doi-asserted-by":"crossref","first-page":"4171","DOI":"10.3390\/molecules24224171","article-title":"Phenolic characterization, antioxidant activity, and enzyme inhibitory properties of Berberis thunbergii DC. leaves: A valuable source of phenolic acids","volume":"24","author":"MD Fern\u00e1ndez-Poyatos","year":"2019","journal-title":"Molecules"},{"issue":"2","key":"pone.0311549.ref031","doi-asserted-by":"crossref","first-page":"325","DOI":"10.3390\/molecules22020325","article-title":"Analysis of the total biflavonoids extract from Selaginella doederleinii by HPLC-QTOF-MS and its in vitro and in vivo anticancer effects","volume":"22","author":"H Yao","year":"2017","journal-title":"Molecules"},{"issue":"7","key":"pone.0311549.ref032","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1016\/j.phytochem.2008.01.026","article-title":"Fingerprint profile of Ginkgo biloba nutritional supplements by LC\/ESI-MS\/MS","volume":"69","author":"S Ding","year":"2008","journal-title":"Phytochemistry"},{"issue":"3","key":"pone.0311549.ref033","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1093\/chromsci\/bmw176","article-title":"The metabolic profiling of isorhamnetin-3-O-neohesperidoside produced by human intestinal flora employing UPLC-Q-TOF\/MS","volume":"55","author":"LY Du","year":"2017","journal-title":"Journal of chromatographic science"},{"issue":"12","key":"pone.0311549.ref034","doi-asserted-by":"crossref","first-page":"3222","DOI":"10.3390\/molecules23123222","article-title":"Rapid characterization and identification of chemical constituents in gentiana radix before and after wine-processed by UHPLC-LTQ-orbitrap MSn","volume":"23","author":"X Lv","year":"2018","journal-title":"Molecules"},{"issue":"12","key":"pone.0311549.ref035","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1002\/jssc.201901169","article-title":"Metabolic profiling comparison of isovitexin in normal and kidney stone model rats by ultra\u2010high\u2010performance liquid chromatography coupled to quadrupole time\u2010of\u2010flight mass spectrometry","volume":"43","author":"C Meng","year":"2020","journal-title":"Journal of Separation Science"},{"key":"pone.0311549.ref036","first-page":"639","article-title":"Phytochemical characterization of phenolics by LC-MS\/MS and biological evaluation of Ajuga orientalis from Turkey Bangladesh","volume":"10","author":"F G\u00f6\u011fer","year":"2015","journal-title":"J Pharmaol"},{"issue":"6","key":"pone.0311549.ref037","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00425-022-03901-7","article-title":"Screening, cloning and functional characterization of key methyltransferase genes involved in the methylation step of 1-deoxynojirimycin alkaloids biosynthesis in mulberry leaves","volume":"255","author":"J Wan","year":"2022","journal-title":"Planta"},{"issue":"8","key":"pone.0311549.ref038","first-page":"1403","article-title":"Analysis of the secondary metabolite products of Ammi majus and evaluation anti-insect activity","volume":"8","author":"HM Hussein","year":"2016","journal-title":"International journal of pharmacognosy and phytochemical research"},{"issue":"11","key":"pone.0311549.ref039","doi-asserted-by":"crossref","first-page":"20409","DOI":"10.3390\/molecules201119702","article-title":"Sensitive and rapid UHPLC-MS\/MS for the analysis of tomato phenolics in human biological samples","volume":"20","author":"M Mart\u00ednez-Hu\u00e9lamo","year":"2015","journal-title":"Molecules"},{"key":"pone.0311549.ref040","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.indcrop.2014.10.068","article-title":"Analysis of phenolic compounds in leaves from endemic trees from Madeira Island. A contribution to the chemotaxonomy of Laurisilva forest species","volume":"64","author":"EJ Llorent-Mart\u00ednez","year":"2015","journal-title":"Industrial Crops and Products"},{"issue":"1","key":"pone.0311549.ref041","first-page":"143","article-title":"Phenolic compounds as marker compounds for botanical origin determination of German propolis samples based on TLC and TLC-MS","volume":"86","author":"J Bertrams","year":"2013","journal-title":"Journal of Applied Botany and Food Quality"},{"issue":"2","key":"pone.0311549.ref042","doi-asserted-by":"crossref","first-page":"389","DOI":"10.3390\/molecules25020389","article-title":"Analysis of flavonoids in dalbergia odorifera by ultra-performance liquid chromatography with tandem mass spectrometry","volume":"25","author":"X Zhao","year":"2020","journal-title":"Molecules"},{"key":"pone.0311549.ref043","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.indcrop.2016.06.007","article-title":"Ulex europaeus: from noxious weed to source of valuable isoflavones and flavanones","volume":"90","author":"V Sp\u00ednola","year":"2016","journal-title":"Industrial Crops and Products"},{"key":"pone.0311549.ref044","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jpba.2017.08.033","article-title":"Development of a sensitive LC\u2013MS\/MS method for quantification of coniferyl ferulate and its metabolite coniferyl alcohol in rat plasma: Application to a pharmacokinetic study","volume":"146","author":"X Dai","year":"2017","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"issue":"2","key":"pone.0311549.ref045","first-page":"793","article-title":"UPLC-ESI-MS\/MS profile of the ethyl acetate fraction of aerial parts of Bougainvillea\u2019Scarlett O\u2019Hara\u2019cultivated in Egypt","volume":"64","author":"M El-sayed","year":"2021","journal-title":"Egyptian Journal of Chemistry"},{"issue":"5","key":"pone.0311549.ref046","doi-asserted-by":"crossref","first-page":"986","DOI":"10.3390\/molecules23050986","article-title":"Antioxidative, antiproliferative and antimicrobial activities of phenolic compounds from three Myrcia species","volume":"23","author":"C Dos Santos","year":"2018","journal-title":"Molecules"},{"issue":"2","key":"pone.0311549.ref047","doi-asserted-by":"crossref","DOI":"10.1177\/1934578X1601100227","article-title":"Profiling of gallic and ellagic acid derivatives in different plant parts of Terminalia arjuna by HPLC-ESI-QTOF-MS\/MS","volume":"11","author":"A Singh","year":"2016","journal-title":"Natural product communications"},{"issue":"1","key":"pone.0311549.ref048","doi-asserted-by":"crossref","first-page":"174","DOI":"10.3390\/molecules22010174","article-title":"A method for LC-MS\/MS profiling of coumarins in Zanthoxylum zanthoxyloides (Lam.) B. Zepernich and Timler extracts and essential oils.","volume":"22","author":"Y Tine","year":"2017","journal-title":"Molecules"},{"key":"pone.0311549.ref049","first-page":"1","article-title":"Recent advances in the one-pot synthesis of coumarin derivatives from different starting materials using nanoparticles: a review","author":"VM Adimule","year":"2022","journal-title":"Topics in Catalysis"},{"issue":"6","key":"pone.0311549.ref050","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1002\/rcm.2897","article-title":"A fragmentation study of isoflavones in negative electrospray ionization by MSn ion trap mass spectrometry and triple quadrupole mass spectrometry","volume":"21","author":"J Kang","year":"2007","journal-title":"Rapid Communications in Mass Spectrometry: An International Journal Devoted to the Rapid Dissemination of Up\u2010to\u2010the\u2010Minute Research in Mass Spectrometry"},{"issue":"1","key":"pone.0311549.ref051","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3390\/molecules23010151","article-title":"Rapid screening and identification of daidzein metabolites in rats based on UHPLC-LTQ-orbitrap mass spectrometry coupled with data-mining technologies","volume":"23","author":"W Zhao","year":"2018","journal-title":"Molecules"},{"issue":"1","key":"pone.0311549.ref052","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jpha.2011.09.009","article-title":"Characterization of flavonoids in Milletti a nitida var. hirsutissima by HPLC\/DAD\/ESI-MSn","volume":"2","author":"M Ye","year":"2012","journal-title":"Journal of pharmaceutical analysis"},{"issue":"11","key":"pone.0311549.ref053","doi-asserted-by":"crossref","first-page":"329","DOI":"10.3390\/separations9110329","article-title":"Standards-based UPLC-Q-exactive orbitrap MS systematically identifies 36 bioactive compounds in ampelopsis grossedentata (vine tea)","volume":"9","author":"R Cai","year":"2022","journal-title":"Separations"},{"issue":"11","key":"pone.0311549.ref054","first-page":"1652","article-title":"UPLC-QTOF-MS E-Based Metabolic Profile to Screening Candidates of Biomarkers of Dwarf-Cashew Clones Resistant and Susceptible to Anthracnose (Colletotrichum gloeosporioides (Penz) Penz. & Sacc.)","volume":"34","author":"PC Alves","year":"2023","journal-title":"Journal of the Brazilian Chemical Society"},{"issue":"1","key":"pone.0311549.ref055","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3390\/molecules25010103","article-title":"Distribution of flavan-3-ol species in ripe strawberry fruit revealed by matrix-assisted laser desorption\/ionization-mass spectrometry imaging","volume":"25","author":"H Enomoto","year":"2019","journal-title":"Molecules"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0311549","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T13:33:41Z","timestamp":1733405621000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0311549"}},"subtitle":[],"editor":[{"given":"Andrea","family":"Mastinu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,5]]},"references-count":55,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0311549","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,5]]}}}