{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T03:52:45Z","timestamp":1794369165590,"version":"4.9.0"},"reference-count":87,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T00:00:00Z","timestamp":1729209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science, Research and Innovation Fund","award":["B16F640175"],"award-info":[{"award-number":["B16F640175"]}]},{"name":"Rachadapisek Sompote Matching Fund","award":["RA-MF-22\/65 and RA-MF-13\/66"],"award-info":[{"award-number":["RA-MF-22\/65 and RA-MF-13\/66"]}]},{"name":"Thailand Science Research and Innovation Fund, Chulalongkorn University","award":["HEA663000017"],"award-info":[{"award-number":["HEA663000017"]}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"crossref","award":["811\/2563"],"award-info":[{"award-number":["811\/2563"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Scholarship from the Graduate School, Chulalongkorn University to commemorate the 72nd anniversary of His Majesty King Bhumibol Aduladej"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Introduction<\/jats:title>\n<jats:p>Despite the well-established effects of aging on brain function and gut dysbiosis (an imbalance in gut microbiota), the influence of aging on sepsis-associated encephalopathy (SAE) and the role of probiotics in this context remain less understood.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>C57BL\/6J mice (8-week-old) were subcutaneously administered with 8 weeks of D-galactose (D-gal) or phosphate buffer solution (PBS) for aging and non-aging models, respectively, with or without 8 weeks of oral <jats:italic>Lacticaseibacillus rhamnosus<\/jats:italic> GG (LGG). Additionally, the impact of the condition media from LGG (LCM) was tested in macrophages (RAW 264.7 cells), microglia (BV-2 cells), and hippocampal cells (HT-22 cells).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Result<\/jats:title>\n<jats:p>Fecal microbiome analysis demonstrated D-gal-induced dysbiosis (reduced Firmicutes and Desulfobacterota with increased Bacteroidota and Verrucomicrobiota), which LGG partially neutralized the dysbiosis. D-gal also worsens cecal ligation and puncture (CLP) sepsis severity when compared with PBS-CLP mice, as indicated by serum creatinine (Scr) and alanine transaminase (ALT), but not mortality, neurological characteristics (SHIRPA score), and serum cytokines (TNF-\u03b1 and IL-6). Additionally, D-gal-induced aging was supported by fibrosis in the liver, kidney, and lung; however, CLP sepsis did not worsen fibrosis. Interestingly, LGG attenuated all parameters (mortality, Scr, ALT, SHIRPA, and cytokines) in non-aging sepsis (PBS-CLP) while improving all these parameters, except for mortality and serum IL-6, in aging sepsis (D-gal CLP). For the in vitro test using lipopolysaccharide (LPS) stimulation, LCM attenuated inflammation in some parameters on RAW264.7 cells but not BV-2 and HT-22 cells, implying a direct anti-inflammatory effect of LGG on macrophages, but not in cells from the brain.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>D-gal induced fecal dysbiosis and worsened sepsis severity as determined by Scr and ALT, and LGG could alleviate most of the selected parameters of sepsis, including SAE. However, the impact of LGG on SAE was not a direct delivery of beneficial molecules from the gut to the brain but partly due to the attenuation of systemic inflammation through the modulation of macrophages.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0311774","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T17:27:37Z","timestamp":1729272457000},"page":"e0311774","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Aging-induced dysbiosis worsens sepsis severity but is attenuated by probiotics in D-galactose-administered mice with cecal ligation and puncture 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Moslem","year":"2016","journal-title":"Journal of Medical Bacteriology"},{"issue":"3","key":"pone.0311774.ref023","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3390\/microorganisms4030035","article-title":"Biofilm Forming Lactobacillus: New Challenges for the Development of Probiotics.","volume":"4","author":"MJ Salas-Jara","year":"2016","journal-title":"Microorganisms"},{"key":"pone.0311774.ref024","doi-asserted-by":"crossref","first-page":"110863","DOI":"10.1016\/j.nut.2020.110863","article-title":"Probiotic Lactobacillus rhamnosus GG reduces mortality of septic mice by modulating gut microbiota composition and metabolic profiles","volume":"78","author":"L Chen","year":"2020","journal-title":"Nutrition"},{"key":"pone.0311774.ref025","doi-asserted-by":"crossref","first-page":"e17427","DOI":"10.7717\/peerj.17427","article-title":"Lactobacillus rhamnosus GG improves cognitive impairments in mice with sepsis.","volume":"12","author":"L Wang","year":"2024","journal-title":"PeerJ."},{"issue":"12","key":"pone.0311774.ref026","doi-asserted-by":"crossref","first-page":"6786","DOI":"10.1002\/fsn3.2630","article-title":"Lactobacillus rhamnosus GG as dietary supplement improved survival from lipopolysaccharides-induced sepsis in mice.","volume":"9","author":"KC Tsui","year":"2021","journal-title":"Food Sci Nutr"},{"issue":"4","key":"pone.0311774.ref027","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.jmii.2020.03.029","article-title":"Lactobacillus rhamnosus sepsis associated with probiotic therapy in an extremely preterm infant: Pathogenesis and a review for clinicians","volume":"54","author":"MC Chiang","year":"2021","journal-title":"J Microbiol Immunol Infect"},{"issue":"1","key":"pone.0311774.ref028","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s13613-023-01233-7","article-title":"The critically ill older patient with sepsis: a narrative review.","volume":"14","author":"M Ibarz","year":"2024","journal-title":"Ann Intensive Care"},{"key":"pone.0311774.ref029","doi-asserted-by":"crossref","first-page":"561652","DOI":"10.3389\/fimmu.2020.561652","article-title":"Candida Administration Worsens Cecal Ligation and Puncture-Induced Sepsis in Obese Mice Through Gut Dysbiosis Enhanced Systemic Inflammation, Impact of Pathogen-Associated Molecules From Gut Translocation and Saturated Fatty Acid.","volume":"11","author":"W Panpetch","year":"2020","journal-title":"Front Immunol."},{"issue":"21","key":"pone.0311774.ref030","doi-asserted-by":"crossref","DOI":"10.3390\/ijms222111450","article-title":"Interference on Cytosolic DNA Activation Attenuates Sepsis Severity: Experiments on Cyclic GMP-AMP Synthase (cGAS) Deficient Mice.","volume":"22","author":"P Visitchanakun","year":"2021","journal-title":"Int J Mol Sci."},{"issue":"5","key":"pone.0311774.ref031","doi-asserted-by":"crossref","first-page":"e135","DOI":"10.1002\/cpz1.135","article-title":"SHIRPA as a 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mice.","volume":"13","author":"L Wu","year":"2022","journal-title":"Food & Function."},{"issue":"3","key":"pone.0311774.ref040","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1038\/s41396-021-01105-7","article-title":"Verrucomicrobiota are specialist consumers of sulfated methyl pentoses during diatom blooms","volume":"16","author":"LH Orellana","year":"2022","journal-title":"The ISME Journal"},{"issue":"8","key":"pone.0311774.ref041","doi-asserted-by":"crossref","first-page":"4326","DOI":"10.1111\/1462-2920.15614","article-title":"Genomic characterization of three novel Desulfobacterota classes expand the metabolic and phylogenetic diversity of the phylum","volume":"23","author":"CL Murphy","year":"2021","journal-title":"Environ Microbiol"},{"issue":"22","key":"pone.0311774.ref042","doi-asserted-by":"crossref","first-page":"4803","DOI":"10.1016\/j.cell.2023.08.017","article-title":"Genetic manipulation of Patescibacteria provides mechanistic insights into microbial dark matter and the epibiotic lifestyle","volume":"186","author":"Y Wang","year":"2023","journal-title":"Cell"},{"issue":"6","key":"pone.0311774.ref043","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1159\/000516348","article-title":"BAM15, a Mitochondrial Uncoupling Agent, Attenuates Inflammation in the LPS Injection Mouse Model: An Adjunctive Anti-Inflammation on Macrophages and Hepatocytes.","volume":"13","author":"CP Dang","year":"2021","journal-title":"J Innate Immun"},{"key":"pone.0311774.ref044","doi-asserted-by":"crossref","first-page":"114948","DOI":"10.1016\/j.bcp.2022.114948","article-title":"The new mitochondrial uncoupler BAM15 induces ROS production for treatment of acute myeloid leukemia","volume":"198","author":"ZX Gao","year":"2022","journal-title":"Biochem Pharmacol"},{"issue":"4\u20135","key":"pone.0311774.ref045","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/S0171-2985(96)80029-5","article-title":"The role of macrophages in septic shock","volume":"195","author":"TJ Evans","year":"1996","journal-title":"Immunobiology"},{"key":"pone.0311774.ref046","doi-asserted-by":"crossref","first-page":"3218452","DOI":"10.1155\/2022\/3218452","article-title":"Metformin Improves the Prognosis of Adult Mice with Sepsis-Associated Encephalopathy Better than That of Aged Mice","volume":"2022","author":"G Song","year":"2022","journal-title":"J Immunol Res"},{"issue":"15","key":"pone.0311774.ref047","article-title":"Aquilariae Lignum Methylene Chloride Fraction Attenuates IL-1\u03b2-Driven Neuroinflammation in BV2 Microglial Cells.","volume":"21","author":"JS Lee","year":"2020","journal-title":"Int J Mol Sci."},{"issue":"7673","key":"pone.0311774.ref048","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/nature24016","article-title":"ApoE4 markedly exacerbates tau-mediated neurodegeneration in a mouse model of tauopathy","volume":"549","author":"Y Shi","year":"2017","journal-title":"Nature"},{"issue":"6","key":"pone.0311774.ref049","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1164\/rccm.201708-1559OC","article-title":"Bacterial Dissemination to the Brain in Sepsis","volume":"197","author":"BH Singer","year":"2018","journal-title":"Am J Respir Crit Care Med"},{"issue":"2","key":"pone.0311774.ref050","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s12028-019-00759-8","article-title":"Brain Volume Changes in Patients with Acute Brain Dysfunction Due to Sepsis.","volume":"32","author":"G Orhun","year":"2020","journal-title":"Neurocrit Care"},{"issue":"1","key":"pone.0311774.ref051","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s12035-014-8868-5","article-title":"Activated Microglia-Induced Deficits in Excitatory Synapses Through IL-1\u03b2: Implications for Cognitive Impairment in Sepsis","volume":"52","author":"CA Moraes","year":"2015","journal-title":"Mol 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disruption by a PKC- and MAP kinase-dependent mechanism","volume":"294","author":"A Seth","year":"2008","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"issue":"4","key":"pone.0311774.ref073","doi-asserted-by":"crossref","first-page":"C1018","DOI":"10.1152\/ajpcell.00131.2005","article-title":"Soluble factors from Lactobacillus GG activate MAPKs and induce cytoprotective heat shock proteins in intestinal epithelial cells","volume":"290","author":"Y Tao","year":"2006","journal-title":"Am J Physiol Cell Physiol"},{"issue":"4","key":"pone.0311774.ref074","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1046\/j.1462-5822.2003.t01-1-00275.x","article-title":"Lactobacillus rhamnosus GG decreases TNF-alpha production in lipopolysaccharide-activated murine macrophages by a contact-independent mechanism","volume":"5","author":"JA Pe\u00f1a","year":"2003","journal-title":"Cell 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Chen","year":"2023","journal-title":"Med Rev"},{"issue":"3","key":"pone.0311774.ref085","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1128\/JCM.03216-14","article-title":"Validation of a new Aspergillus real-time PCR assay for direct detection of Aspergillus and azole resistance of Aspergillus fumigatus on bronchoalveolar lavage fluid","volume":"53","author":"GL Chong","year":"2015","journal-title":"J Clin Microbiol"},{"key":"pone.0311774.ref086","doi-asserted-by":"crossref","first-page":"1244321","DOI":"10.3389\/fcell.2023.1244321","article-title":"Lessons in aging from Myc knockout mouse models","volume":"11","author":"EV Prochownik","year":"2023","journal-title":"Front Cell Dev Biol"},{"issue":"1","key":"pone.0311774.ref087","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/s42003-024-06386-0","article-title":"Apoptosis signal-regulating kinase 1 promotes inflammation in senescence and aging.","volume":"7","author":"T 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