{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T05:44:22Z","timestamp":1741153462795,"version":"3.38.0"},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["NoZR2020QH208"],"award-info":[{"award-number":["NoZR2020QH208"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Shandong Medical and Health Technology Development Plan","award":["No202101041037"],"award-info":[{"award-number":["No202101041037"]}]},{"name":"The Shandong First Medical University College Student Innovation and Entrepreneurship Training Program","award":["2022104391726"],"award-info":[{"award-number":["2022104391726"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>\nObjective\n<\/jats:title>\n<jats:p>To investigate the changes of <jats:bold>Cold-Inducible RNA-Binding Protein<\/jats:bold> (CIRBP) expression in mouse spinal cord injury model.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>\nMethods\n<\/jats:title>\n<jats:p>Seventy-five female C57BL\/6 mice were randomly divided into five groups, 15 mice per group. According to different degrees of spinal cord injury, they were divided into Mild spinal cord injury, Moderate spinal cord injury, Severe spinal cord injury, Spinal cord amputation group, and Sham surgery group, all constructed with spinal cord percussion. All groups were dissected 1, 3, 5, 14, and 21 days after modeling. HE staining was used to observe the pathological changes in the spinal cord, The Basso mouse scale (BMS) was used for motor function scoring, and immunofluorescence was used to detect the expression of NeuN, IBA-1, and CIRBP in spinal cord tissues.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>\nResults\n<\/jats:title>\n<jats:p>HE results showed that inflammation was more pronounced in moderate, severe, and amputation injuries compared to the Sham surgery group. Moderate injury group and Severe injury group inflammation increased consistently over time. The severe injury group had severe tissue structure destruction and increased astrocytes significantly. Combined with the mouse BMS motor function score, the mouse severe injury group model was more stable. Compared with the Sham surgery group, there was a significant decrease in NeuN over time (P &lt; 0.01) and a significant increase in IBA-1 and CIRBP (P &lt; 0.01) in the severe injury group. Moreover, IBA-1 has significant co-localization with CIRBP.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>\nConclusion\n<\/jats:title>\n<jats:p>CIRBP expression is significantly elevated in a mouse spinal cord injury model, Which may be related to the proliferation of microglia during spinal cord injury.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0311803","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T18:34:32Z","timestamp":1741113272000},"page":"e0311803","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression of cold-inducible RNA-binding protein in mouse spinal cord injury model"],"prefix":"10.1371","volume":"20","author":[{"given":"Xinhui","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingkun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wentao","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengli","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shutong","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5006-2483","authenticated-orcid":true,"given":"Yueying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"issue":"3S","key":"pone.0311803.ref001","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1093\/neuros\/nyw080","article-title":"Traumatic spinal cord injury-repair and regeneration","volume":"80","author":"CS Ahuja","year":"2017","journal-title":"Neurosurgery"},{"issue":"1","key":"pone.0311803.ref002","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1038\/s41392-022-00885-4","article-title":"Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury","volume":"7","author":"C Li","year":"2022","journal-title":"Signal Transduct Target Ther"},{"issue":"5660","key":"pone.0311803.ref003","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1126\/science.1093133","article-title":"Nanotubular highways for intercellular organelle transport","volume":"303","author":"A Rustom","year":"2004","journal-title":"Science"},{"issue":"4","key":"pone.0311803.ref004","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/s12015-017-9730-8","article-title":"Characterization of tunneling nanotubes in Wharton\u2019s jelly mesenchymal stem cells. an intercellular exchange of components between neighboring cells","volume":"13","author":"V Sanchez","year":"2017","journal-title":"Stem Cell Rev Rep"},{"issue":"1","key":"pone.0311803.ref005","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s12987-020-00213-4","article-title":"Chronic extradural compression of spinal cord leads to syringomyelia in rat model","volume":"17","author":"L Ma","year":"2020","journal-title":"Fluids Barriers CNS"},{"issue":"1","key":"pone.0311803.ref006","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/JLB.3MIR1118-443R","article-title":"Extracellular CIRP (eCIRP) and inflammation","volume":"106","author":"M Aziz","year":"2019","journal-title":"J Leukoc Biol"},{"issue":"1","key":"pone.0311803.ref007","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00441-018-2871-5","article-title":"Mesenchymal stromal cell-derived extracellular vesicles: regenerative and immunomodulatory effects and potential applications in sepsis","volume":"374","author":"G Zheng","year":"2018","journal-title":"Cell Tissue Res"},{"key":"pone.0311803.ref008","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.wneu.2021.07.148","article-title":"Inflammatory response to spinal cord injury and its treatment","volume":"155","author":"X Liu","year":"2021","journal-title":"World Neurosurg"},{"issue":"23","key":"pone.0311803.ref009","doi-asserted-by":"crossref","first-page":"e2201319","DOI":"10.1002\/adhm.202201319","article-title":"Inflammatory microenvironment-responsive nanomaterials promote spinal cord injury repair by targeting IRF5","volume":"11","author":"D Ma","year":"2022","journal-title":"Adv Healthc Mater"},{"key":"pone.0311803.ref010","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.brainres.2013.01.041","article-title":"Effects of moderate and deep hypothermia on RNA-binding proteins RBM3 and CIRP expressions in murine hippocampal brain slices","author":"G Tong","year":"2013","journal-title":"Brain Res"},{"issue":"2","key":"pone.0311803.ref011","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S2215-0366(21)00395-3","article-title":"Global, regional, and national burden of 12 mental disorders in 204 countries and territories, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019","volume":"9","author":"GBD 2019 Mental Disorders Collaborators","year":"2022","journal-title":"Lancet Psychiatry"},{"key":"pone.0311803.ref012","doi-asserted-by":"crossref","first-page":"106728","DOI":"10.1016\/j.intimp.2020.106728","article-title":"Cold-inducible RNA-binding protein (CIRBP) promotes porcine reproductive and respiratory syndrome virus (PRRSV)-induced inflammatory response","volume":"86","author":"X Xiao","year":"2020","journal-title":"Int Immunopharmacol"},{"issue":"2","key":"pone.0311803.ref013","doi-asserted-by":"crossref","DOI":"10.1002\/wrna.1462","article-title":"Cold\u2010inducible RNA binding protein in cancer and inflammation","volume":"9","author":"DA Lujan","year":"2018","journal-title":"WIREs RNA"},{"key":"pone.0311803.ref014","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.intimp.2016.07.007","article-title":"Cold-inducible RNA-binding protein mediates cold air inducible airway mucin production through TLR4\/NF-\u03baB signaling pathway","volume":"39","author":"L Chen","year":"2016","journal-title":"Int Immunopharmacol"},{"key":"pone.0311803.ref015","doi-asserted-by":"crossref","first-page":"41363","DOI":"10.1038\/srep41363","article-title":"Cold-inducible RNA-binding protein (CIRP) causes sepsis-associated acute lung injury via induction of endoplasmic reticulum stress","volume":"7","author":"MM Khan","year":"2017","journal-title":"Sci Rep"},{"issue":"4","key":"pone.0311803.ref016","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.surg.2017.06.004","article-title":"Deficiency in cold-inducible RNA-binding protein attenuates acute respiratory distress syndrome induced by intestinal ischemia-reperfusion","volume":"162","author":"C Cen","year":"2017","journal-title":"Surgery"},{"issue":"203","key":"pone.0311803.ref017","article-title":"Automated impactor for contusive spinal cord injury model in mice","author":"M Wu","year":"2024","journal-title":"J Vis Exp"},{"issue":"11\u201312","key":"pone.0311803.ref018","first-page":"1238","article-title":"Effects of ischemia and H2O2 on the cold stress protein CIRP expression in rat neuronal cells","volume":"27","author":"JH Xue","year":"1999","journal-title":"Free Radic Biol Med"},{"key":"pone.0311803.ref019","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.brainres.2014.11.061","article-title":"Cloning, expression, and purification of cold inducible RNA-binding protein and its neuroprotective mechanism of action","author":"J Liu","year":"2015","journal-title":"Brain Res"},{"issue":"7","key":"pone.0311803.ref020","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1016\/j.bbagen.2014.02.027","article-title":"Cold-inducible RNA-binding protein mediates neuroinflammation in cerebral ischemia","author":"M Zhou","year":"2014","journal-title":"Biochim Biophys Acta"},{"issue":"11","key":"pone.0311803.ref021","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1002\/ijc.30833","article-title":"The role of cold-inducible RNA binding protein in cell stress response","volume":"141","author":"Y Liao","year":"2017","journal-title":"Int J Cancer"},{"issue":"1","key":"pone.0311803.ref022","doi-asserted-by":"crossref","first-page":"e12949","DOI":"10.1111\/sji.12949","article-title":"Cold-inducible RNA-binding protein (CIRP) in inflammatory diseases: molecular insights of its associated signalling pathways","volume":"93","author":"P Zhong","year":"2021","journal-title":"Scand J Immunol"},{"issue":"4","key":"pone.0311803.ref023","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1111\/jcmm.17142","article-title":"The role of Cold-Inducible RNA-binding protein in respiratory diseases","volume":"26","author":"P Zhong","year":"2022","journal-title":"J Cell Mol Med"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0311803","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T18:34:46Z","timestamp":1741113286000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0311803"}},"subtitle":[],"editor":[{"given":"Jian","family":"Hao","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,4]]},"references-count":23,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0311803","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,4]]}}}