{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T12:33:10Z","timestamp":1766579590151,"version":"3.30.2"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Graduate Studies and Scientific Research at Qassim University","award":["QU-APC-2024-9\/1"],"award-info":[{"award-number":["QU-APC-2024-9\/1"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>A Partial pulpotomy technique is an alternative choice of treatment for immature permanent teeth with exposed vital pulps. This study aimed to compare the histopathological pulpal response of the primary teeth of puppies after partial pulpotomy and full pulpotomy using mineral trioxide aggregate (MTA).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Materials and methods<\/jats:title>\n<jats:p>72 primary premolars from experimental puppies aged 6\u20138 weeks were equally divided into test and control groups, as MTA partial pulpotomy (MTA-PP) and MTA full pulpotomy (MTA-FP). After 60 days, the teeth were extracted and examined histologically. The specimens were assessing the extent and intensity of inflammatory response (acute or chronic), necrosis, fibrosis, calcific bridge (presence or absence), pulp calcification, and pathological root resorption.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>The Inflammation intensity was lower (2.8%) in MTA-PP than in MTA-FP (16.7%) (P = 0.004). MTA-FP also had a significantly higher percentage of localized (27.8%) and diffuse (11.1%) necrosis (P = 0.014), whereas both groups (P = 0.290) had an equal fibrosis degree. A calcific bridge was observed in 72.2% of cases. Meanwhile, pathological internal root resorption was more evident in MTA-FP (63.9%) than in MTA-PP (19.4%) (P &lt;0.0001).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Partial MTA pulpotomy procedure demonstrated promising histological findings that can be applied to vital-pulp therapy in primary teeth.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0312076","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T18:41:12Z","timestamp":1734028872000},"page":"e0312076","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pulpal response to partial pulpotomy versus full pulpotomy procedures in puppies: An experimental study"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1493-9492","authenticated-orcid":true,"given":"Thiyezen Abdullah","family":"AlDhelai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8681-3429","authenticated-orcid":true,"given":"Zeyad","family":"Alsughier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faraj","family":"Alotaiby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa Hussein","family":"Alattas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7586-4319","authenticated-orcid":true,"given":"Muhammad Qasim","family":"Javed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madeh","family":"Sadan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rania M.","family":"Salem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mona A.","family":"Elkateb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"key":"pone.0312076.ref001","first-page":"457","volume-title":"Pulp Therapy for Primary and Immature Permanent Teeth. The Reference Manual of Pediatric Dentistry","author":"AAPD","year":"2023"},{"issue":"5","key":"pone.0312076.ref002","first-page":"359","article-title":"Clinical and Radiographic Success of Low-Level Laser Therapy Compared with Formocresol Pulpotomy Treatment in Primary Molars","volume":"42","author":"N Alamoudi","year":"2020","journal-title":"Pediatric dentistry"},{"issue":"1","key":"pone.0312076.ref003","first-page":"16","article-title":"Primary Tooth Vital Pulp Therapy: A Systematic Review and Meta-analysis","volume":"39","author":"JA Coll","year":"2017","journal-title":"Pediatric dentistry"},{"issue":"2","key":"pone.0312076.ref004","first-page":"139","article-title":"A retrospective study of a modified 1-minute formocresol pulpotomy technique part 2: effect on exfoliation times and successors","volume":"33","author":"ZA Kurji","year":"2011","journal-title":"Pediatric dentistry"},{"issue":"2","key":"pone.0312076.ref005","first-page":"60","article-title":"A histopathological comparison of pulpotomy with sodium hypochlorite and formocresol","volume":"7","author":"R Haghgoo","year":"2012","journal-title":"Iranian endodontic journal"},{"issue":"3","key":"pone.0312076.ref006","doi-asserted-by":"crossref","first-page":"385","DOI":"10.4103\/0970-9290.87058","article-title":"Comparative evaluation of formocresol and mineral trioxide aggregate as pulpotomy agents in deciduous teeth","volume":"22","author":"D Srinivasan","year":"2011","journal-title":"Indian journal of dental research"},{"issue":"5","key":"pone.0312076.ref007","doi-asserted-by":"crossref","DOI":"10.1002\/14651858.CD003220.pub3","article-title":"Pulp treatment for extensive decay in primary teeth","volume":"5","author":"V Sma\u00efl-Faugeron","year":"2018","journal-title":"The Cochrane database of systematic reviews"},{"issue":"10","key":"pone.0312076.ref008","article-title":"Vital Pulp Therapy in Primary Dentition: Pulpotomy-A 100-Year Challenge","volume":"8","author":"A. Igna","year":"2021","journal-title":"Children (Basel, Switzerland)"},{"issue":"8","key":"pone.0312076.ref009","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/S0099-2399(78)80153-8","article-title":"A clinical report on partial pulpotomy and capping with calcium hydroxide in permanent incisors with complicated crown fracture","volume":"4","author":"M. Cvek","year":"1978","journal-title":"Journal of endodontics"},{"issue":"1","key":"pone.0312076.ref010","first-page":"29","article-title":"Partial pulpotomy for immature permanent teeth, its present and future","volume":"24","author":"CD Fong","year":"2002","journal-title":"Pediatric dentistry"},{"issue":"11","key":"pone.0312076.ref011","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1016\/j.joen.2019.07.005","article-title":"Outcome of Partial Pulpotomy in Cariously Exposed Posterior Permanent Teeth: A Systematic Review and Meta-analysis","volume":"45","author":"F Elmsmari","year":"2019","journal-title":"Journal of endodontics"},{"issue":"6","key":"pone.0312076.ref012","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/j.1600-9657.1987.tb00639.x","article-title":"A one-year follow-up of partial pulpotomy and calcium hydroxide capping in primary molars","volume":"3","author":"U Schr\u00f6der","year":"1987","journal-title":"Endodontics & dental traumatology"},{"issue":"11","key":"pone.0312076.ref013","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1016\/j.joen.2017.06.025","article-title":"Human Pulp Responses to Partial Pulpotomy Treatment with TheraCal as Compared with Biodentine and ProRoot MTA: A Clinical Trial","volume":"43","author":"H Bakhtiar","year":"2017","journal-title":"Journal of endodontics"},{"issue":"3","key":"pone.0312076.ref014","first-page":"184","article-title":"Mineral Trioxide Aggregate Partial Pulpotomy Versus Formocresol Pulpotomy: A Randomized, Split-Mouth, Controlled Clinical Trial with 24 Months Follow-Up","volume":"40","author":"H Nematollahi","year":"2018","journal-title":"Pediatric dentistry"},{"key":"pone.0312076.ref015","first-page":"47","article-title":"Partial Versus Complete Pulpotomy Using Three Different Materials in Primary Molars: A Clinical Study","author":"A Abdelhafez","year":"2022"},{"issue":"2","key":"pone.0312076.ref016","first-page":"83","article-title":"Histopathologic Responses of Dog\u2019s Dental Pulp to Mineral Trioxide Aggregate, Bio Active Glass, Formocresol, Hydroxyapatite","volume":"4","author":"E Jabbarifar","year":"2008","journal-title":"Dental Research Journal"},{"issue":"4","key":"pone.0312076.ref017","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1177\/089875649100800402","article-title":"The modified Triadan system: nomenclature for veterinary dentistry","volume":"8","author":"MR Floyd","year":"1991","journal-title":"Journal of veterinary dentistry"},{"key":"pone.0312076.ref018","volume-title":"Histology, Staining. StatPearls","author":"TS Gurina","year":"2023"},{"key":"pone.0312076.ref019","first-page":"858","volume-title":"Veterinary Anatomy of Domestic Mammals: Textbook and Colour Atlas.","author":"HE K\u00f6nig","year":"2020","edition":"7"},{"issue":"3","key":"pone.0312076.ref020","doi-asserted-by":"crossref","first-page":"222","DOI":"10.5005\/jp-journals-10005-1368","article-title":"Success Rate of MTA Pulpotomy on Vital Pulp of Primary Molars: A 3-Year Observational Study","volume":"9","author":"B Godhi","year":"2016","journal-title":"International journal of clinical pediatric dentistry"},{"issue":"6","key":"pone.0312076.ref021","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.5005\/jp-journals-10024-1443","article-title":"MM-MTA for direct pulp capping: a histologic comparison with ProRoot MTA in rat molars","volume":"14","author":"IT Khalil","year":"2013","journal-title":"The journal of contemporary dental practice"},{"issue":"8","key":"pone.0312076.ref022","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.joen.2015.04.007","article-title":"Comparative Study of Pulpal Responses to Pulpotomy with ProRoot MTA, RetroMTA, and TheraCal in Dogs\u2019 Teeth","volume":"41","author":"H Lee","year":"2015","journal-title":"Journal of endodontics"},{"issue":"6","key":"pone.0312076.ref023","article-title":"Effects of Three Calcium Silicate Cements on Inflammatory Response and Mineralization-Inducing Potentials in a Dog Pulpotomy Model","volume":"11","author":"CM Kang","year":"2018","journal-title":"Materials (Basel, Switzerland)"},{"key":"pone.0312076.ref024","doi-asserted-by":"crossref","first-page":"9582165","DOI":"10.1155\/2020\/9582165","article-title":"The Response of the Pulp-Dentine Complex, PDL, and Bone to Three Calcium Silicate-Based Cements: A Histological Study in an Animal Rat Model","volume":"2020","author":"R Mahmood Talabani","year":"2020","journal-title":"Bioinorganic chemistry and applications"},{"issue":"4","key":"pone.0312076.ref025","first-page":"302","article-title":"Comparison of mineral trioxide aggregate and formocresol as pulp-capping agents in pulpotomized primary teeth","volume":"26","author":"HA Agamy","year":"2004","journal-title":"Pediatric dentistry"},{"key":"pone.0312076.ref026","first-page":"226","volume-title":"McDonald and Avery\u2019s Dentistry for the Child and Adolescent.","author":"JA Dean","year":"2022","edition":"11"},{"issue":"3","key":"pone.0312076.ref027","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/j.1600-9657.2005.00311.x","article-title":"A comparative study of white and grey mineral trioxide aggregate as pulp capping agents in dog\u2019s teeth.","volume":"21","author":"M Parirokh","year":"2005","journal-title":"Dental traumatology: official publication of International Association for Dental Traumatology"},{"issue":"22","key":"pone.0312076.ref028","article-title":"Macrophages and Wnts in Tissue Injury and Repair","volume":"11","author":"MH Park","year":"2022","journal-title":"Cells"},{"key":"pone.0312076.ref029","first-page":"329","volume-title":"Pediatric Dentistry\u2014Infancy Through Adolescence","author":"AB Fuks","year":"2019","edition":"6"},{"key":"pone.0312076.ref030","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jdent.2018.04.007","article-title":"Changes in the radicular pulp-dentine complex in healthy intact teeth and in response to deep caries or restorations: A histological and histobacteriological study","volume":"73","author":"D Ricucci","year":"2018","journal-title":"Journal of dentistry"},{"issue":"6","key":"pone.0312076.ref031","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1046\/j.1600-9657.2003.00204.x","article-title":"Comparison of bioactive glass, mineral trioxide aggregate, ferric sulfate, and formocresol as pulpotomy agents in rat molar","volume":"19","author":"N Salako","year":"2003","journal-title":"Dental traumatology: official publication of International Association for Dental Traumatology"},{"issue":"7","key":"pone.0312076.ref032","first-page":"291","article-title":"Reaction of the rat molar pulp to various materials","volume":"122","author":"AH Rowe","year":"1967","journal-title":"British dental journal"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0312076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T18:41:29Z","timestamp":1734028889000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0312076"}},"subtitle":[],"editor":[{"given":"Carlos Alberto Antunes","family":"Viegas","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,12]]},"references-count":32,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0312076","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,12]]}}}