{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:41:37Z","timestamp":1791873697480,"version":"4.3.4"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Foundation of Guizhou Provincial Health Commission","award":["gzwkj2022-159"],"award-info":[{"award-number":["gzwkj2022-159"]}]},{"name":"Science and Technology Plan Project of Zunyi City Science and Technology and Big Data Bureau","award":["HZ2022-58"],"award-info":[{"award-number":["HZ2022-58"]}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["82160200"],"award-info":[{"award-number":["82160200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Technology Plan Project of Guizhou Provincial Science and Technology Department","award":["ZK2024-086"],"award-info":[{"award-number":["ZK2024-086"]}]},{"DOI":"10.13039\/501100014965","name":"Do\u011fa Koruma Ve Milli Parklar Genel M\u00fcd\u00fcrl\u00fc\u011f\u00fc Te\u015fkilat Yapisi","doi-asserted-by":"publisher","award":["82460212"],"award-info":[{"award-number":["82460212"]}],"id":[{"id":"10.13039\/501100014965","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Diabetic retinopathy (DR) is a leading cause of blindness. We hypothesised that the long non-coding RNA <jats:italic>RP11-502I4.3<\/jats:italic> may be involved in angiogenesis associated with DR. We investigated the role of <jats:italic>RP11-502I4.3<\/jats:italic> in DR by examining its regulation of vascular endothelial growth factor (VEGF). We assessed differences in <jats:italic>RP11-502I4.3<\/jats:italic> expression between the control group and streptozotocin-induced diabetic rats or high glucose (HG)-stimulated human retinal microvascular endothelial cells (HRMECs). VEGF expression was measured with and without lentiviral vectors overexpressing <jats:italic>RP11-502I4.3<\/jats:italic>. We analysed the structural alterations related to DR after overexpressing <jats:italic>RP11-502I4.3<\/jats:italic>. Our analysis revealed that <jats:italic>RP11-502I4.3<\/jats:italic> expression was lower in the retinas of diabetic rats and HG-stimulated HRMECs compared with normal glucose conditions. Overexpressing of <jats:italic>RP11-502I4.3<\/jats:italic> resulted in decreased VEGF levels. Diabetic rats exhibited retinopathy characterised by thinning of the retinal layer thickness, structural changes in the inner and outer nuclear layers, a reduced count of retinal ganglion cells, and the presence of acellular capillaries. The proliferative activity, migration count, and tube formation ability of HG-treated HRMECs were significantly higher than those of the control group. However, these changes were inhibited by <jats:italic>RP11-502I4.3<\/jats:italic> overexpression. Overexpression <jats:italic>RP11-502I4.3<\/jats:italic> might inhibit retinopathy of diabetic rats and HG-induced angiogenesis by downregulating VEGF expression.<\/jats:p>","DOI":"10.1371\/journal.pone.0312791","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T17:32:48Z","timestamp":1747243968000},"page":"e0312791","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Targeting long non-coding RNA RP11-502I4.3 inhibits the trend of angiogenesis in diabetic 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