{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T23:50:15Z","timestamp":1782172215765,"version":"3.54.5"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Future Medicine 2030 Project of the Samsung Medical Center","award":["SMX1240031"],"award-info":[{"award-number":["SMX1240031"]}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["RS-2024-00332244"],"award-info":[{"award-number":["RS-2024-00332244"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The genus <jats:italic>Enterococcus<\/jats:italic> is increasingly recognized for its involvement in various human infections, with several species known to be pathogenic. This study characterized <jats:italic>Enterococcus<\/jats:italic> sp. SMC-9, isolated from bile of a patient with cholangitis, and compared its characteristics with those of <jats:italic>Enterococcus montenegrensis<\/jats:italic> CoE-012-22<jats:sup>T<\/jats:sup>, recently isolated from dried beef sausage. A comprehensive analysis, encompassing phylogenetic, genomic, and phenotypic studies, confirmed that strain SMC-9 belongs to the same species as <jats:italic>E<\/jats:italic>. <jats:italic>montenegrensis<\/jats:italic> CoE-012-22<jats:sup>T<\/jats:sup>. However, comparative genomic analysis revealed key differences in virulence and antibiotic resistance gene profiles between the two strains. Notably, genes related to exopolysaccharide biosynthesis and the L-rhamnose biosynthesis pathway were found exclusively in strain SMC-9, suggesting their role in the strain\u2019s colonization of the biliary tract and its involvement in cholangitis. Additionally, the tetracycline resistance gene <jats:italic>tet(M)<\/jats:italic>, which was absent in <jats:italic>E<\/jats:italic>. <jats:italic>montenegrensis<\/jats:italic> CoE-012-22<jats:sup>T<\/jats:sup>, was identified in strain SMC-9, explaining its high tetracycline minimum inhibitory concentration (&gt;16 \u03bcg\/mL). These findings highlight the unique pathogenic traits of strain SMC-9 compared to <jats:italic>E<\/jats:italic>. <jats:italic>montenegrensis<\/jats:italic> CoE-012-22<jats:sup>T<\/jats:sup>. Our study underscores the significant genetic and phenotypic variations that can exist among strains within the same species, highlighting the critical need for strain typing to assess their potential impact on patient outcomes and public health.<\/jats:p>","DOI":"10.1371\/journal.pone.0312953","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T18:33:56Z","timestamp":1733164436000},"page":"e0312953","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of an Enterococcus sp. 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