{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:42:52Z","timestamp":1783518172693,"version":"3.55.0"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T00:00:00Z","timestamp":1735603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82003473"],"award-info":[{"award-number":["82003473"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Recently researches have reported that miRNA and its target genes are associated with osteoporosis. MiRNA and mRNA might be potential diagnostic markers for osteoporosis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Purposes<\/jats:title>\n<jats:p>The aim of this study is to explore the potential miRNA and mRNA markers by bioinformatics method and clinical analysis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Patients and methods<\/jats:title>\n<jats:p>The miRNA expression profiles were obtained from GSE74209, GSE64433 and GSE115773 in Gene expression Omnibus (GEO). The mRNA expression profiles were obtained from GSE100609. Wayne intersection were used to explore the different expressed miRNAs (DE-miRs). Select the miRNA with the highest Fold Change for subsequent research. Screening of miRNA target genes using TargetScan and miRDB tools. GO and KEGG analyses of target genes (TGs) function were performed. Validate the selected TGs in the GSE100609. We collected female patients with femural intertrochanteric fractures from July 1, 2023 to October 31, 2023. Patient\u2019s bone marrow and clinical data were collected. MiRNA and the target mRNA differentially expressed in bone marrow were verified through RT-qPCR. All data were subjected to Shapiro-Wilk test. Using Pearson or Spearman test to detect the correlation between various indicators, and then incorporating indicators related to bone density into multiple linear regression equations. Partial correlation analysis was used to analyze the correlation between the final indicators and bone density.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Results<\/jats:title>\n<jats:p>A total of 140 DE-miRs were identified. Set the fold change to \u201c&gt;1\u201d and ultimately include 5 miRNAs. Using miR-595 (highest |log<jats:sub>2<\/jats:sub> FC|) as the subject of subsequent research. 3542 targeted mRNAs were predicted from TargetScan and 362 were from miRDB. 337 TGs were intersected, which were mainly enriched in nucleus. Only Cldnd1 were identified using the GSE100609 dataset. We found that miR-595 was highly expressed in patients with high bone mass, while Cldnd1 was downregulated. There was a strong collinearity between miR-595 and Cldnd1. Further multiple linear regression analysis showed a high correlation between miR-595 and bone density.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>These data suggest that miR-595 and Cldnd1 might be related factors for decreased bone mass.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0313106","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T18:39:06Z","timestamp":1735670346000},"page":"e0313106","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["MiR-595 and Cldnd1: Potential related factors for bone loss in postmenopausal women with hip osteoporotic fracture"],"prefix":"10.1371","volume":"19","author":[{"given":"Sun","family":"Jingyue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Peixin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6249-6895","authenticated-orcid":true,"given":"Wang","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,31]]},"reference":[{"issue":"11","key":"pone.0313106.ref001","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.1002\/jbmr.1692","article-title":"Iron overload accelerates bone loss in healthy postmenopausal women and middle-aged men: a 3-year retrospective longitudinal study","volume":"27","author":"BJ Kim","year":"2012","journal-title":"J Bone Miner Res"},{"issue":"10","key":"pone.0313106.ref002","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1080\/03630242.2023.2278211","article-title":"Estrogen hormone therapy and postmenopausal osteoporosis: does it really take two to tango?","volume":"63","author":"BC Silva","year":"2023","journal-title":"Women Health"},{"issue":"11","key":"pone.0313106.ref003","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1007\/s40618-023-02082-8","article-title":"A systematic review on the performance of fracture risk assessment tools: FRAX, DeFRA, FRA-HS","volume":"46","author":"G Adami","year":"2023","journal-title":"J Endocrinol Invest"},{"issue":"1","key":"pone.0313106.ref004","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s11657-023-01347-2","article-title":"Targeted optimum care approach for osteoporotic fragility fractures: tailored strategy based on risk stratification to reduce incidents of falls-an initiative by the Egyptian Academy of bone health based on the FLS national register","volume":"18","author":"Y El Miedany","year":"2023","journal-title":"Arch Osteoporos"},{"key":"pone.0313106.ref005","article-title":"Presence of subchondral fracture in cases diagnosed as transient osteoporosis of the hip: a retrospective independent reader-based study.","volume":"7","author":"A Gulati","year":"2023","journal-title":"Skeletal Radiol."},{"key":"pone.0313106.ref006","first-page":"17","article-title":"Identification of Conserved and Novel MicroRNAs with their Targets in Garden Pea (Pisum Sativum L.) 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