{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T01:12:43Z","timestamp":1789434763717,"version":"build-2803163510"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Provincial Undergraduate Universities in Heilongjiang Province and Cooperative Innovation and Extension System for Heilongjiang Modern Agricultural Industry Technology of Pig","award":["YQTH2023182"],"award-info":[{"award-number":["YQTH2023182"]}]},{"name":"Study on the Biological Response Mechanism of Pigs and Chickens to Music Amplitude","award":["32172788"],"award-info":[{"award-number":["32172788"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The purpose of this study was to explore whether music can reduce stress in animals by regulating the activity of the hypothalamus pituitary adrenal cortex (HPA) axis and reducing the concentration of cortisol. The control group was not played with any music or mechanical noise, the music group played music before and during transport, and the noise group played noise before and during transport as a positive control. The results showed that after two-hours of transportation, the concentrations of adrenocorticotropic hormone and cortisol in the music group were lower than that in the control and the noise groups, while the cortisol level in the noise group was higher than that in the music and the control groups. Plasma concentrations of D-lactic acid and diamine oxidase in the music group were lower than those in the control group and the noise group, and the noise group was higher than the control group. In addition, the concentrations of jejunal inflammatory factors interleukin-6 and interleukin-8 in the music group were lower than those in the control group and the noise group, but there was no difference of interleukin-12 in the three groups. However, there was no significant difference in Illinois-6, Illinois-12 and INF-\u03b3 between the noise group and the control group. The contents of reactive oxygen species, malondialdehyde and glutathione peroxidase in the music group had no changes compared with those in the control group, while the contents of reactive oxygen species and malondialdehyde in the noise group were higher than the control and the music groups, and the content of glutathione peroxidase was decreased. Compared with the music group, differently expressed genes analysis also showed that the mRNA expression level of inflammatory genes in the jejunum of the music group\u2019 piglets decreased. In addition, compared with the music group, some Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to inflammation were highly expressed in the control group. In conclusion, our results showed that musical stimulation can reduce the inflammatory response of piglets caused by transportation by reducing the activity of HPA axis. Noise increased the activity of HPA axis, which aggravated the intestinal damage of piglets and caused intestinal oxidative damage<\/jats:p>","DOI":"10.1371\/journal.pone.0313354","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T18:29:32Z","timestamp":1740508172000},"page":"e0313354","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["A new therapy to alleviate the inflammatory injury of piglet intestine caused by short-distance 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exposure on rat immunity","volume":"63","author":"X She","year":"2021","journal-title":"J Occup Health"},{"issue":"8","key":"pone.0313354.ref036","doi-asserted-by":"crossref","first-page":"2581","DOI":"10.2527\/jas.2011-4444","article-title":"Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs","volume":"90","author":"LH Zhu","year":"2012","journal-title":"J Anim Sci"},{"issue":"3","key":"pone.0313354.ref037","doi-asserted-by":"crossref","first-page":"e0120485","DOI":"10.1371\/journal.pone.0120485","article-title":"Trolox and ascorbic acid reduce direct and indirect oxidative stress in the IPEC-J2 cells, an in vitro model for the porcine gastrointestinal tract","volume":"10","author":"H Vergauwen","year":"2015","journal-title":"PLoS One"},{"key":"pone.0313354.ref038","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jad.2016.03.021","article-title":"Thyroid peroxidase autoantibodies and perinatal depression risk: a 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noise-induced oxidative stress in rat serum?","volume":"17","author":"ER Koc","year":"2015","journal-title":"Noise Health"},{"issue":"38","key":"pone.0313354.ref042","doi-asserted-by":"crossref","first-page":"3528","DOI":"10.1093\/eurheartj\/ehy333","article-title":"Crucial role for Nox2 and sleep deprivation in aircraft noise-induced vascular and cerebral oxidative stress, inflammation, and gene regulation","volume":"39","author":"S Kr\u00f6ller-Sch\u00f6n","year":"2018","journal-title":"Eur Heart J"},{"issue":"11","key":"pone.0313354.ref043","doi-asserted-by":"crossref","first-page":"3634","DOI":"10.2527\/jas.2010-3751","article-title":"Intestinal growth and morphology is associated with the increase in heat shock protein 70 expression in weaning piglets through supplementation with glutamine","volume":"89","author":"X Zhong","year":"2011","journal-title":"J Anim 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cells[J]","volume":"23","author":"Z Chen","year":"2018","journal-title":"Molecules"},{"issue":"5","key":"pone.0313354.ref047","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s10863-020-09850-8","article-title":"CADM1 enhances intestinal barrier function in a rat model of mild inflammatory bowel disease by inhibiting the STAT3 signaling pathway[J]","volume":"52","author":"S Sun","year":"2020","journal-title":"J Bioenerg Biomembr"},{"key":"pone.0313354.ref048","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cbpa.2016.06.012","article-title":"Examining the relationships between egg cortisol and oxidative stress in developing wild sockeye salmon (Oncorhynchus nerka)","volume":"200","author":"JJ Taylor","year":"2016","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"issue":"1","key":"pone.0313354.ref049","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1080\/10253890.2022.2132142","article-title":"Protective effects of nanocurcumin 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